Antibodies to Epstein-Barr Virus in Nasopharyngeal Carcinoma, Other Head and Neck Neoplasms, and Control Groups<xref ref-type="fn" rid="FN2">2</xref>

Because antibody titers against Epstein-Barr virus (EBV) antigens often are elevated in patients with active undifferentiated and nonkeratinizing nasopharyngeal carcinoma, anti-EBV serologic tests have been applied as a diagnostic aid in patients who have metastatic lymphadenopathy in the neck without an obvious source. In this study of 44 patients, the serologic testing procedure proved useful in identifying six patients with nasopharyngeal carcinoma that was initially occult but was eventually confirmed by biopsy.

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Possibility of the use of serological method for the determination of immunoglobuline a antibody against early antigene of Epstein-Barr virus as a marker in the diagnosis of nasopharyngeal tumors

Vojnosanitetski pregled ◽

10.2298/vsp0510739s ◽

2005 ◽

Vol 62 (10) ◽

pp. 739-744 ◽

Cited By ~ 3

Author(s):

Svetlana Stosic-Divjak ◽

Vojko Djukic ◽

Zeljko Petrovic ◽

Vladimir Nesic ◽

Alek Racic ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Laryngeal Carcinoma ◽

Epstein Barr Virus ◽

Blood Donors ◽

Close Association ◽

Undifferentiated Carcinoma ◽

Control Groups ◽

Serological Method ◽

Barr Virus ◽

Epstein Barr

Background/Aim. According to the data from immunological, biological and molecular researches, there is a close association between the undifferentiated carcinoma of nasopharyngeal type (UCNT) and Epstein-Barr virus (EBV). To use IgA EA antibody as a serological marker in our patients with nasopharyngeal carcinoma from a clinical viewpoint. Methods. 91 patients were followed in the period from 1989?1998. In 11 of the patients the antibody titre serum for the early antigen of EBV virus were determinated before the treatment, and in 24 of the patients 3 years after the treatment. There were three control groups of patients: 20 voluntary blood donors, 26 patients with squamocellular laryngeal carcinoma, and 10 patients with squamocellular nasopharyngeal carcinoma. Results. In the group of 11 patients with UCNT before the treatment, the value of anti-EA IgA titre was 31.09, and in the patients after the treatment anti-EA IgA antiody titre was 14.56. In the control groups of patients the results were: in the blood donors 5.00; in the group with the diagnosis of squamocellular laryngeal carcinoma, the titre was 5.00; in the patients with squamocellular nosopharyngeal carcinoma, the titre anti-EA IgA was 5.36. Conclusion. These results were statistically highly significant (p < 0.01). Our research clearly showed that anti-EA IgA EBV marker could be useful in diagnosing, differential diagnosing and prognosing as well.

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Association of Epstein–Barr Virus and Cytomegalovirus Infections with Esophageal Carcinoma

International Journal of Cancer Management ◽

10.5812/ijcm.114566 ◽

2021 ◽

Vol 14 (10) ◽

Author(s):

Mahin Ahangar Oskouee ◽

Jamal Sarvari ◽

Afagh Moattari ◽

Ahmad Habibi ◽

Amir Taher Eftehkar Sadat

Keyword(s):

Esophageal Carcinoma ◽

Epstein Barr Virus ◽

Viral Infections ◽

Control Group ◽

Control Groups ◽

Barr Virus ◽

Potential Association ◽

Epstein Barr ◽

And Control ◽

Cytomegalovirus Infections

Background: Given the fact that viral infections play an important role, either directly or indirectly, in around 20 percent of human cancers, this study aimed at investigating the potential association of Epstein–Barr virus (EBV) and cytomegalovirus (CMV) infections in esophageal cancer that is the sixth most common cause of cancer-related deaths. Methods: In this case-control study, a total of 200 paraffin-embedded biopsies of cancerous and benign esophageal tissues were gathered from the biopsy bank of Imam Reza Hospital, Tabriz, Iran in 2017. All samples were first deparaffinized, and then subjected to commercial DNA extraction. The quality of extracted DNA was evaluated by amplification of the beta globulin gene. Identification of EBV and CMV DNA was performed using primers designed for the EBER region of EBV and the immediate early (IE) region of the CMV genome, respectively. Results: The mean age of the subjects in the test and control groups was 52.2 (17.1) and 59.9 (18.9), respectively. The distribution of gender (male/female) in patient and control groups was 54/46 and 53/47, respectively. Our results showed that the frequency of EBV (P < 0.001) and CMV (P < 0.001) in cancerous samples was statistically higher than control group. Moreover, in the cancerous group the rate of EBV was significantly higher in the esophageal adenocarcinomas (EAC) sample (12 out of 70) than esophageal squamous cell carcinomas (ESCC) (0 out of 30) (P = 0.016) but, in the ESCC group, 17 out of 30 subjects were positive for CMV which was significantly higher in comparison with EAC patients (1 out of 70) (P < 0.001). Conclusions: Findings indicated that EBV and CMV might be contributed to the pathogenesis of EAC and ESCC types of esophageal carcinoma, respectively, although further studies are warranted.

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Expression of Epstein–Barr virus lytic gene BRLF1 in nasopharyngeal carcinoma: potential use in diagnosis

Journal of General Virology ◽

10.1099/0022-1317-81-10-2417 ◽

2000 ◽

Vol 81 (10) ◽

pp. 2417-2423 ◽

Cited By ~ 43

Author(s):

Ping Feng ◽

Ee Chee Ren ◽

Dingxiang Liu ◽

Soh Ha Chan ◽

Huaizhong Hu

Keyword(s):

Nasopharyngeal Carcinoma ◽

Epstein Barr Virus ◽

Tumour Cells ◽

Plasma Samples ◽

Igg Antibodies ◽

Barr Virus ◽

Epstein Barr ◽

And Control ◽

Lytic Genes

Tumour cells of undifferentiated nasopharyngeal carcinoma (NPC) consistently harbour Epstein–Barr virus (EBV) genes. Expression of mRNA transcripts associated with EBV latency has been demonstrated in such cells. However, expression of EBV lytic genes has not been well elucidated, although various lines of evidence have suggested that there is EBV replication in NPC tumour cells. We have studied mRNA expression of representative EBV lytic genes by RT–PCR in nasopharynx biopsies obtained from NPC and control individuals. In both NPC and control biopsies, EBV lytic genes BZLF1, BALF2 and BCLF1 were detected readily. However, BRLF1 was detected in NPC biopsies only. The BRLF1 gene was then cloned and expressed in vitro, and the protein product, Rta, was used as an antigen to detect specific antibodies by immunoprecipitation in plasma samples obtained from NPC patients and healthy controls. IgG antibodies directed against Rta were detected in 44 of 53 NPC plasma samples (83·0%), but only in 1 of 53 control samples (1·9%). Furthermore, the antibody binding regions were found in the C-terminal two-thirds of Rta. This serological result confirms indirectly that BRLF1 is specifically expressed in NPC tumour cells. Rta might play an important role in NPC pathogenesis, considering its multiple functions in EBV replication and cell cycles. Moreover, the detection of IgG antibodies directed against Rta could be developed into a diagnostic parameter for NPC.

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Epstein-Barr virus serology in nasopharyngeal carcinomas and other head and neck neoplasms in Italy

Journal of Cancer Research and Clinical Oncology ◽

10.1007/bf00402731 ◽

1985 ◽

Vol 110 (2) ◽

pp. 157-160 ◽

Cited By ~ 3

Author(s):

A. Faggioni ◽

C. Corradini ◽

G. Barile ◽

G. Cardi ◽

V. Ciarniello ◽

...

Keyword(s):

Head And Neck ◽

Head And Neck Neoplasms ◽

Epstein Barr Virus ◽

Barr Virus ◽

Epstein Barr

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Molecular Detection of Epstein–Barr Virus, Human Herpes Virus 6, Cytomegalovirus, and Hepatitis B Virus in Patients with Multiple Sclerosis

Middle East Journal of Digestive Diseases ◽

10.34172/mejdd.2020.179 ◽

2020 ◽

Vol 12 (3) ◽

pp. 171-177

Author(s):

Mohsen Asouri ◽

Mohammad Ali Sahraian ◽

Morteza Karimpoor ◽

Sadegh Fattahi ◽

Nima Motamed ◽

...

Keyword(s):

Epstein Barr Virus ◽

Control Group ◽

Case Group ◽

Control Groups ◽

Barr Virus ◽

Human Herpes Virus ◽

B Virus ◽

Human Herpes Virus 6 ◽

Epstein Barr ◽

And Control

BACKGROUND Multiple sclerosis (MS) is a chronic disease with significant morbidity. A wide spectrum of risk factors has been suggested that triggers the development of MS. Among them, several viral infections have been implicated to play a role in MS pathogenesis. We aimed to evaluate the relationship between viral diseases, including Epstein–Barr virus (EBV), human herpes virus 6 (HHV-6), cytomegalovirus (CMV), and hepatitis B virus (HBV) and MS in the present case-control study. METHODS About 100 patients with confirmed MS and age- and sex-matched individuals were selected as case and control groups, respectively. The patients were randomly selected from individuals diagnosed by neurologists based on the clinical signs and symptoms and imaging procedures. RESULTS More than 100 patients with MS and patients who were referred for other causes were analyzed for the presence of DNA of EBV, HHV6, CMV, and HBV separately. 9.37% of the control group had a positive test for the DNA of EBV in a real-time polymerase chain reaction (PCR), while the frequency of positive test result was zero in the case group (p = 0.0012). HBV DNA was not detected in both the case and control groups. The prevalence of CMV was 0.88 and zero in the control and case groups, respectively (p = 0.3410). For HHV6, 9.73 % of the control group had a positive result, while this test was positive in 5.88% of the patients with MS (p = 0.2959). CONCLUSION We detected a significantly higher number of individuals with DNA of EBV in their blood among the control group compared with the case group. In conclusion, the results suggest a surprisingly adverse association between MS and EBV, and no association was found between the presence of DNA of HBV, CMV, and HHV6 and MS.

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