The Human Leukemic HL-60 Cell Line: An in Vitro Model for Cell Death Endpoint Identification

The aim of this study was to investigate the endpoints related to cell death, either necrosis or apoptosis, induced by four chemicals in the promyelocytic leukemia cell line, HL-60. Cell morphology, DNA fragmentation, cytofluorimetric analysis and oxygen consumption were used to classify the type of cell death observed. In our analysis, we found that not all the selected parameters reproduced the differences observed in the cell death caused by the four chemicals tested. As cell death is a very complex phenomenon, several factors should be taken into account (cell type, exposure time and chemical concentration), if chemicals are to be classified according to differences in the mechanisms more directly involved in cell death.

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The HL-60 promyelocytic leukemia cell line: proliferation, differentiation, and cellular oncogene expression

Blood ◽

10.1182/blood.v70.5.1233.1233 ◽

1987 ◽

Vol 70 (5) ◽

pp. 1233-1244 ◽

Cited By ~ 791

Author(s):

SJ Collins

Keyword(s):

Cell Line ◽

Leukemia Cell ◽

Promyelocytic Leukemia ◽

Leukemic Cells ◽

Leukemia Cell Line ◽

Molecular Events ◽

Proliferation And Differentiation ◽

Vitro Model ◽

Promyelocytic Leukemia Cell Line

Abstract The HL-60 cell line, derived from a single patient with acute promyelocytic leukemia, provides a unique in vitro model system for studying the cellular and molecular events involved in the proliferation and differentiation of normal and leukemic cells of the granulocyte/monocyte/macrophage lineage.

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The HL-60 promyelocytic leukemia cell line: proliferation, differentiation, and cellular oncogene expression

Blood ◽

10.1182/blood.v70.5.1233.bloodjournal7051233 ◽

1987 ◽

Vol 70 (5) ◽

pp. 1233-1244 ◽

Cited By ~ 9

Author(s):

SJ Collins

Keyword(s):

Cell Line ◽

Leukemia Cell ◽

Promyelocytic Leukemia ◽

Leukemic Cells ◽

Leukemia Cell Line ◽

Molecular Events ◽

Proliferation And Differentiation ◽

Vitro Model ◽

Promyelocytic Leukemia Cell Line

The HL-60 cell line, derived from a single patient with acute promyelocytic leukemia, provides a unique in vitro model system for studying the cellular and molecular events involved in the proliferation and differentiation of normal and leukemic cells of the granulocyte/monocyte/macrophage lineage.

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Normal functional characteristics of cultured human promyelocytic leukemia cells (HL-60) after induction of differentiation by dimethylsulfoxide.

Journal of Experimental Medicine ◽

10.1084/jem.149.4.969 ◽

1979 ◽

Vol 149 (4) ◽

pp. 969-974 ◽

Cited By ~ 398

Author(s):

S J Collins ◽

F W Ruscetti ◽

R E Gallagher ◽

R C Gallo

Keyword(s):

Leukemia Cell ◽

Myeloid Cell ◽

Promyelocytic Leukemia ◽

Leukemia Cell Line ◽

Functional Characteristics ◽

Dye Reduction ◽

Normal Functional ◽

Vitro Model ◽

Promyelocytic Leukemia Cell Line

The HL-60 human promyelocytic leukemia cell line can be induced to terminally differentiate to mature myeloid cells sharing a number of functional characteristics with normal granulocytes including response to chemoattractants, development of complement receptors, phagocytosis, superoxide production, and nitroblue tetrazolium dye reduction. Hence the Me2SO-induced HL-60 cells provide a unique in vitro model for studying various important aspects of human myeloid cell differentiation.

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Ultrastructural localization of F-actin using phalloidin and quantum dots in HL-60 promyelocytic leukemia cell line after cell death induction by arsenic trioxide

Acta Histochemica ◽

10.1016/j.acthis.2012.11.005 ◽

2013 ◽

Vol 115 (5) ◽

pp. 487-495 ◽

Cited By ~ 13

Author(s):

Magdalena Izdebska ◽

Maciej Gagat ◽

Dariusz Grzanka ◽

Alina Grzanka

Keyword(s):

Quantum Dots ◽

Cell Death ◽

Cell Line ◽

Arsenic Trioxide ◽

Leukemia Cell ◽

Ultrastructural Localization ◽

Promyelocytic Leukemia ◽

Leukemia Cell Line ◽

Promyelocytic Leukemia Cell Line

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Characteristic expression of glycosphingolipid profiles in the bipotential cell differentiation of human promyelocytic leukemia cell line HL-60

Blood ◽

10.1182/blood.v64.2.534.534 ◽

1984 ◽

Vol 64 (2) ◽

pp. 534-541 ◽

Cited By ~ 56

Author(s):

H Nojiri ◽

F Takaku ◽

T Tetsuka ◽

K Motoyoshi ◽

Y Miura ◽

...

Keyword(s):

Cell Differentiation ◽

Cell Line ◽

Molecular Species ◽

Leukemia Cell ◽

Promyelocytic Leukemia ◽

Leukemia Cell Line ◽

Myeloid Differentiation ◽

Human Promyelocytic Leukemia Cell ◽

Promyelocytic Leukemia Cell Line

Abstract Changes of glycosphingolipids (GSLs) in the bipotential cell differentiation of human promyelocytic leukemia cell line HL-60 cells were investigated by high-performance thin-layer chromatography (HPTLC), with special reference to morphological and functional changes, such as phagocytosis and nitroblue tetrazolium (NBT) reduction. Nine molecular species of neutral GSLs and 13 or more species of sialo-GSLs, ie, gangliosides, were detected on the HPTLC chromatograms for untreated HL-60 cells. The major components were ceramide dihexoside (CDH), GM3, and sialo-paragloboside (SPG). When HL- 60 cells were induced to differentiate into both myeloid mature cells and macrophage-like cells in vitro, no new molecular species of GSLs specific for one of the cell differentiations was induced, but distinctive quantitative changes in the GSL composition were definitely observed between the two cell differentiations. During the myeloid differentiation induced by either dimethylsulfoxide (DMSO) or retinoic acid (RA), CDH, paragloboside (PG), and gangliosides having longer sugar moieties characteristically increased with a concomitant decrease of GSLs with shorter sugar chains, such as ceramide monohexoside (CMH) and GM3, and the GSL composition profile of myeloid differentiation- induced HL-60 cells became more similar to that of normal human granulocytes. However, some marked differences were noted between the induced HL-60 cells and the normal granulocytes, especially in the ganglioside compositions. These differences might reflect either some deficiency in the in vitro myeloid differentiation or some leukemic properties of HL-60 cells. In marked contrast to the change of GSL composition during myeloid differentiation, a remarkable increase of GM3, with a concurrent marked decrease of CDH, was observed in the process of cell differentiation into macrophage-like cells with 12-O- tetradecanoyl-phorbol-13-acetate (TPA), which suggested an increase in the biosynthesis of GM3. These results demonstrate that HL-60 cells express distinct GSL profiles, depending not only on maturation stages but also on differentiation directions.

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Metabolomics identifies the intersection of phosphoethanolamine with menaquinone-triggered apoptosis in an in vitro model of leukemia

Molecular BioSystems ◽

10.1039/c5mb00237k ◽

2015 ◽

Vol 11 (9) ◽

pp. 2406-2416 ◽

Cited By ~ 14

Author(s):

Suganthagunthalam Dhakshinamoorthy ◽

Nha-Truc Dinh ◽

Jeffrey Skolnick ◽

Mark P. Styczynski

Keyword(s):

Cell Line ◽

Proliferative Activity ◽

In Vitro Model ◽

Leukemia Cell ◽

Leukemia Cell Line ◽

Vitro Model

We characterize the anti-proliferative activity of menaquinone in a leukemia cell line and use metabolomics to link it to phosphoethanolamine.

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Characteristic expression of glycosphingolipid profiles in the bipotential cell differentiation of human promyelocytic leukemia cell line HL-60

Blood ◽

10.1182/blood.v64.2.534.bloodjournal642534 ◽

1984 ◽

Vol 64 (2) ◽

pp. 534-541 ◽

Cited By ~ 1

Author(s):

H Nojiri ◽

F Takaku ◽

T Tetsuka ◽

K Motoyoshi ◽

Y Miura ◽

...

Keyword(s):

Cell Differentiation ◽

Cell Line ◽

Molecular Species ◽

Leukemia Cell ◽

Promyelocytic Leukemia ◽

Leukemia Cell Line ◽

Myeloid Differentiation ◽

Human Promyelocytic Leukemia Cell ◽

Promyelocytic Leukemia Cell Line

Changes of glycosphingolipids (GSLs) in the bipotential cell differentiation of human promyelocytic leukemia cell line HL-60 cells were investigated by high-performance thin-layer chromatography (HPTLC), with special reference to morphological and functional changes, such as phagocytosis and nitroblue tetrazolium (NBT) reduction. Nine molecular species of neutral GSLs and 13 or more species of sialo-GSLs, ie, gangliosides, were detected on the HPTLC chromatograms for untreated HL-60 cells. The major components were ceramide dihexoside (CDH), GM3, and sialo-paragloboside (SPG). When HL- 60 cells were induced to differentiate into both myeloid mature cells and macrophage-like cells in vitro, no new molecular species of GSLs specific for one of the cell differentiations was induced, but distinctive quantitative changes in the GSL composition were definitely observed between the two cell differentiations. During the myeloid differentiation induced by either dimethylsulfoxide (DMSO) or retinoic acid (RA), CDH, paragloboside (PG), and gangliosides having longer sugar moieties characteristically increased with a concomitant decrease of GSLs with shorter sugar chains, such as ceramide monohexoside (CMH) and GM3, and the GSL composition profile of myeloid differentiation- induced HL-60 cells became more similar to that of normal human granulocytes. However, some marked differences were noted between the induced HL-60 cells and the normal granulocytes, especially in the ganglioside compositions. These differences might reflect either some deficiency in the in vitro myeloid differentiation or some leukemic properties of HL-60 cells. In marked contrast to the change of GSL composition during myeloid differentiation, a remarkable increase of GM3, with a concurrent marked decrease of CDH, was observed in the process of cell differentiation into macrophage-like cells with 12-O- tetradecanoyl-phorbol-13-acetate (TPA), which suggested an increase in the biosynthesis of GM3. These results demonstrate that HL-60 cells express distinct GSL profiles, depending not only on maturation stages but also on differentiation directions.

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