scholarly journals Circulating exosomal long non-coding RNA H19 as a potential novel diagnostic and prognostic biomarker for gastric cancer

2020 ◽  
Vol 48 (7) ◽  
pp. 030006052093429 ◽  
Author(s):  
Hui Zhou ◽  
Weifeng Shen ◽  
Hongxing Zou ◽  
Qingshan Lv ◽  
Pingyang Shao
Keyword(s):  
Gastric Cancer ◽  
Roc Curves ◽  
Prospective Clinical Study ◽  
Operating Characteristics ◽  
Clinical Role ◽  
Non Coding Rna ◽  
Potential Biomarker ◽  
Before And After ◽  
Auc Value ◽  
Long Non Coding Rna

Objective Long non-coding RNAs (lncRNAs) are involved in carcinogenesis and could be used as diagnostic biomarkers. Our study aimed to elucidate the clinical role of serum exosomal lncRNA H19 in gastric cancer (GC). Methods In this prospective clinical study, we determined serum exosomal lncRNA H19 levels in 81 patients with GC and analysed the correlations between serum lncRNA H19 levels and clinical characteristics. Receiver operating characteristics (ROC) curves were constructed to determine the diagnostic performance of exosomal lncRNA H19 in GC. Results Serum exosomal lncRNA H19 levels were significantly upregulated in patients with GC both before and after surgery compared with healthy controls. Furthermore, serum exosomal lncRNA H19 levels were significantly decreased after compared with before surgery in patients with GC. Preoperative lncRNA H19 levels were significantly correlated with TNM stage. The area under the ROC curve (AUC) value for exosomal lncRNA H19 was 0.849, which was significantly higher than the AUC values for cancer antigens 19-9 and 72-4 and carcinoembryonic antigen, either alone or combined. Conclusions These results suggest that circulating exosomal lncRNA H19 may be a potential biomarker with diagnostic and prognostic value in GC.

scholarly journals LncRNA-ANRIL promotes gastric cancer progression by enhancing NF-kB signaling

2019 ◽  
Vol 244 (12) ◽  
pp. 953-959 ◽  
Author(s):  
Wei Deng ◽  
Yulong Zhang ◽  
Jun Cai ◽  
Jun Zhang ◽  
Xiaoye Liu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Progression ◽  
Cancer Progression ◽  
Additional Treatment ◽  
Cancer Prognosis ◽  
Mechanistic Study ◽  
Major Purpose ◽  
Non Coding Rna ◽  
Potential Biomarker ◽  
Long Non Coding Rna

Metastasis is the most challenging issue for gastric cancer, and identification of the molecular mechanism and suitable targets for treatment is the major purpose of recent research. In this study, we found the long non-coding RNA ANRIL was critical for the progression of gastric cancer. Knockdown of ANRIL (also known as CDKN2B-AS) with shRNA increased apoptosis, inhibited tumor growth, and suppressed migration of cancer cells. TET2 (Tet Methylcytosine Dioxygenase 2), a methylcytosine dioxygenase suppressed ANRIL function and prevented cancer progression. Patients with higher TET2 expression survived better, while with higher ANRIL survived worse. Furthermore, expressions of TET2 and ANRIL were negatively correlated in the patient samples. The mechanistic study suggested that ANRIL promoted tumor progression mainly by enhancing NF-kB signaling. Impact statement Gastric cancer is one of the leading causes of cancer-related death. The lack of curative therapeutic options ascribes to the complex genetic background and heterogeneity of gastric cancer. Understanding the molecular details of the disease and identifying the therapeutic targets would offer additional treatment options. Long non-coding RNA ANRIL was involved in the progression of many cancers, including gastric cancer, but the mechanism was unknown. The current study indicated that ANRIL supported tumor cell survival by inhibiting apoptosis and promoted metastasis by enhancing NF-kB signaling. NF-kB signaling was critical in tumor progression, and this study proved another long non-coding RNA that could regulate NF-kB signaling. ANRIL would be a potential biomarker and therapeutic target for gastric cancer prognosis and treatment.

Evaluating the diagnostic and prognostic value of serum long non-coding RNA CTC-497E21.4 in gastric cancer

2019 ◽  
Vol 57 (7) ◽  
pp. 1063-1072 ◽  
Author(s):  
Wei Zong ◽  
Wei Feng ◽  
Yun Jiang ◽  
Shaoqing Ju ◽  
Ming Cui ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Prognostic Value ◽  
Quantitative Polymerase Chain Reaction ◽  
High Sensitivity ◽  
Expression Levels ◽  
Non Coding Rna ◽  
Potential Biomarker ◽  
Polymerase Chain ◽  
Long Non Coding Rna

Abstract Background Long non-coding RNAs (lncRNAs) have been reported to play a key role in gastric cancer (GC) tumorigenesis. However, the clinical application value of serum lncRNAs in GC has remained largely unknown. We investigated the role of a novel lncRNA named CTC-497E21.4 in the diagnosis and the prognosis of GC. Methods We focused on evaluation of lncRNA CTC-497E21.4 by real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR). The study involved following aspects: (1) confirmation of the higher lncRNA CTC-497E21.4 expression in different types of GC specimens than corresponding controls; (2) evaluation of monitoring tumor dynamics by the serum lncRNA CTC-497E21.4 assay; (3) evaluation of the prognostic value of lncRNA CTC-497E21.4 assay in GC. Results (1) The method of RTFQ-PCR detection of lncRNA CTC-497E21.4 was evaluated to have high sensitivity and specificity. (2) The expression levels of lncRNA CTC-497E21.4 were higher in GC patients compared with corresponding controls (p<0.001), and the combination of serum lncRNA CTC-497E21.4, CEA and CA19-9 could improve diagnostic sensitivity (96.36%). (3) The serum lncRNA CTC-497E21.4 expression levels were lower in postoperative samples than preoperative samples (p=0.0021) and survival curves downloaded from TCGA showed high lncRNA CTC-497E21.4 levels were associated with poor OS of GC (p=0.0351). Conclusions lncRNA CTC-497E21.4 may be a potential biomarker for the diagnosis and the prognosis of GC.

scholarly journals Novel long non-coding RNA RP11-119F7.4 as a potential biomarker for the development and progression of gastric cancer

2015 ◽  
Vol 10 (1) ◽  
pp. 115-120 ◽  
Author(s):  
JINGXU SUN ◽  
YONGXI SONG ◽  
XIAOWAN CHEN ◽  
JUNHUA ZHAO ◽  
PENG GAO ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Non Coding Rna ◽  
Potential Biomarker ◽  
Long Non Coding Rna

The Variant rs145204276 of GAS5 is Associated with the Development and Prognosis of Gastric Cancer

2018 ◽  
Vol 27 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Qianjun Li ◽  
Gang Ma ◽  
Huimin Guo ◽  
Suhua Sun ◽  
Ying Xu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Survival Rate ◽  
Cancer Progression ◽  
Regulatory Mechanism ◽  
Tumor Stage ◽  
Kaplan Meier ◽  
Non Coding Rna ◽  
Early Tumor ◽  
Long Non Coding Rna ◽  
Clinicopathological Variables

Background & Aims: Down-regulation of the growth arrest specific transcript 5 (GAS5) (long non-coding RNA) is associated with cell proliferation of gastric cancer (GC) and a poor prognosis. We aimed to investigate whether the variant rs145204276 of GAS5 is associated with the prognosis of GC in the Chinese population, and to unveil the regulatory mechanism underlying the GAS5 expression in GC tissues.Method: 1,253 GC patients and 1,354 healthy controls were included. The frequency of the genotype del/del and the allele del of rs145204276 were compared between the patients and the controls and between different subgroups of patients classified by clinicopathological variables. The overall survival rate was analyzed according to the Kaplan-Meier method using the log-rank test.Results: The frequency of genotype del/del was significantly lower in patients than in the controls (7.0% vs. 9.1%, p = 0.001). Kaplan-Meier analysis showed that genotype del/del was significantly associated with a higher survival rate (p = 0.01). Patients with late tumor stage were found to have a significantly lower rate of genotype del/del than those with an early tumor stage (4.9% vs. 8.8%, p = 0.01). Patients with UICC III and IV were found to have a significantly lower rate of genotype del/del than those with UICC I and II (5.3% vs. 8.1%, p = 0.02).Conclusion: The variant rs145204276 of GAS5 is associated with the development and prognosis of GC. The allele del of rs145204276 is associated with a remarkably lower incidence of cancer progression and metastasis.

scholarly journals Retraction: Long non-coding RNA XIST promotes proliferation, autophagy and inhibits apoptosis by regulating microRNA-30c/ATG5 axis in gastric cancer

RSC Advances ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 4233-4233
Author(s):  
Laura Fisher
Keyword(s):  
Gastric Cancer ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Retraction of ‘Long non-coding RNA XIST promotes proliferation, autophagy and inhibits apoptosis by regulating microRNA-30c/ATG5 axis in gastric cancer’ by Mingjian Liu et al., RSC Adv., 2018, 8, 37508–37517, DOI: 10.1039/C8RA07852A.

scholarly journals Retraction: Long non-coding RNA GACAT1 alleviates doxorubicin and vincristine resistance through a PTEN/AKT/mTOR/S6K1 regulatory pathway in gastric cancer

RSC Advances ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 5000-5000
Author(s):  
Laura Fisher
Keyword(s):  
Gastric Cancer ◽  
Regulatory Pathway ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Retraction of ‘Long non-coding RNA GACAT1 alleviates doxorubicin and vincristine resistance through a PTEN/AKT/mTOR/S6K1 regulatory pathway in gastric cancer’ by Hengxuan Ding et al., RSC Adv., 2019, 9, 8048–8055, DOI: 10.1039/C8RA10030F.

scholarly journals Long non‐coding RNA KCNQ1OT1 promotes the progression of gastric cancer via the miR‐145‐5p/ARF6 axis

2021 ◽  
Author(s):  
Xiongdong Zhong ◽  
Xiaoyan Wen ◽  
Lei Chen ◽  
Ni Gu ◽  
Xianchang Yu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Non Coding Rna ◽  
Long Non Coding Rna
Sign in / Sign up

Export Citation Format

Share Document