Demonstration of Noradrenaline-Immunoreactive Nerve Fibres in the Liver

1996 ◽  
Vol 24 (6) ◽  
pp. 466-472 ◽  
Author(s):  
Y Fukuda ◽  
M Imoto ◽  
Y Koyama ◽  
Y Miyazawa ◽  
T Hayakawa
Keyword(s):  
Human Liver ◽  
Central Vein ◽  
Nerve Fibres ◽  
Portal Area ◽  
Cacodylate Buffer ◽  
Antibody Method ◽  
Tissue Specimens ◽  
Cryostat Sections ◽  
Distribution Of Noradrenaline ◽  
Liver Tissues

To demonstrate noradrenaline-immunoreactive nerve fibres in liver tissues, we used an antibody to noradrenaline in the immunostaining of liver tissues from rats, guinea-pigs and humans. The tissue specimens were fixed by perfusion or immersion with cacodylate buffer containing sodium metabisulphate and glutaraldehyde, and cryostat sections were prepared. An indirect peroxidase-labelled antibody method was used for staining noradrenaline. Noradrenaline-immunoreactive nerve fibres were localized around blood vessels in the portal area and around the central vein. There were differences between the species in the intralobular distribution of noradrenaline-immunoreactive fibres. Normal guinea-pig and human liver showed intralobular noradrenaline-immunoreactive fibres while rat liver did not. Noradrenaline-immunoreactive fibres were absent from regenerating nodules in a human cirrhotic liver. This method of demonstrating noradrenaline directly using perfusion- or immersion-fixation is appropriate for studying innervation in normal and damaged livers of various species including humans.

scholarly journals Histological insight into the hepatic tissue of the Nile monitor (Varanus niloticus)

2018 ◽  
Vol 2 (3) ◽  
pp. 240-250
Author(s):  
Yasser A. Ahmed ◽  
Mohammed Abdelsabour Khalaf ◽  
Elsaysed Mohammed
Keyword(s):  
Light And Electron Microscopy ◽  
Lymphatic Vessels ◽  
Left Lobe ◽  
Hepatic Tissue ◽  
Histological Structure ◽  
Central Vein ◽  
Bile Canaliculi ◽  
Portal Area ◽  
Space Of Disse ◽  
Liver Fragments

The liver of reptiles is considered an important study model for the interaction between environment and hepatic tissue. Little is known about the histology of the liver of reptiles. The aim of the current study was to elucidate the histological architecture of the liver of the Nile monitor (Varanus niloticus). Liver fragments from the Nile monitor were collected in the summer season and processed for the light and electron microscopy. The liver of the Nile monitor was bi-lobed and the right lobe was found to be larger than the left lobe. Histological examination revealed indistinct lobulation of the liver, and the central vein, sinusoids and portal area were haphazardly organized. The hepatic parenchyma consisted of hepatocytes arranged in glandular-like alveoli or tubules separated by a network of twisted capillary sinusoids. The hepatocytes were polyhedral in shape with vacuolated cytoplasm and the nucleus was single rounded, eccentric, large and vesicular with a distinct nucleolus. The hepatocytes contained numerous lipid droplets, abundant glycogen granules and well-developed RER and mitochondria. The hepatocytes appeared to secrete into the bile canaliculi through the disintegration of their dark cytoplasm into the bile canaliculi. The space of Disse separating between the hepatocytes and sinusoids contained many recesses. The portal area contained branches of the portal vein, hepatic artery, bile duct and lymphatic vessels embedded in a connective tissue. Some non-parenchymal cells were described such as Kupffer cells, heterophils, melano-macrophages, intercalated cells, myofibroblasts in addition to the endothelium of the sinusoids. This is the first report about the histological structure of the liver of the Egyptian Nile monitor. The result presented here should be considered a baseline knowledge to compare with the pathological affections of the liver in this species.

Effects of an Antioxidant Extract on Adenosine Deaminase Activities in Cancerous Human Liver Tissues

2004 ◽  
Vol 1 (1) ◽  
pp. 53-56 ◽  
Author(s):  
A. Avci . ◽  
M. Kacmaz . ◽  
M. Kavutcu . ◽  
E. Gocmen . ◽  
I. Durak .
Keyword(s):  
Adenosine Deaminase ◽  
Human Liver ◽  
Liver Tissues

scholarly journals Immunohistochemical characterisation of neurons in the mandibular ganglion and nerve fibres supplying the porcine mandibular gland

2017 ◽  
Vol 61 (No. 7) ◽  
pp. 361-373 ◽  
Author(s):  
M. Klimczuk ◽  
P. Podlasz ◽  
W. Sienkiewicz ◽  
A. Franke-Radowiecka ◽  
A. Dudek ◽  
...  
Keyword(s):  
Mandibular Gland ◽  
Double Labelling ◽  
Nerve Terminals ◽  
Rt Pcr ◽  
Nerve Fibres ◽  
Chemical Coding ◽  
Pcr Product ◽  
Calcitonin Gene ◽  
Oxide Synthase ◽  
Cryostat Sections

The present study was designed to investigate the chemical coding of neurons in the mandibular ganglion (MGn) and nerve fibres supplying the porcine mandibular gland (MGl) with the use of immunofluorescence and RT-PCR. The cryostat sections from MGn and MGl were processed for double-labelling immunohistochemistry using antisera against vesicular acetylcholine transporter (VAChT), choline acetyltransferase (ChAT), dopamine β-hydroxylase (DβH), neuronal nitric oxide synthase (nNOS), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), galanin (GAL), substance P (SP) and calcitonin gene-related peptide (CGRP). The MGl was found to be richly supplied by VAChT-positive nerve fibres that surrounded intra- and interlobular salivary ducts. A large number of VAChT-immunoreactive (VAChT-IR) nerve terminals were also observed around acini. Many periductal and periacinar nerve fibres stained positive for DβH. Immunoreactivity to GAL, NPY or VIP was observed in an intermediate number of nerve terminals which were associated with both salivary ducts and acini. Double-immunostaining revealed that in MGn nearly all neurons stained positive for VAChT/ChAT (98.45 ± 0.59%, mean ± SEM) and nNOS (99.71 ± 0.18%). An intermediate number of the nerve cell bodies displayed immunoreactivity to NPY or VIP (18.67 ± 0.52% and 8.11 ± 0.36%, respectively). Single GAL-IR and CGRP-positive neurons were also observed. RT-PCR revealed the presence of transcripts of ChAT, VAChT, nNOS, NPY, VIP and GAL. For SP and DβH very weak signals were observed. RT-PCR with primers targeting CGRP did not generate any PCR product.

scholarly journals Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages

2018 ◽  
Vol 28 (5) ◽  
pp. 510-521 ◽  
Author(s):  
Jing Li ◽  
Feiyue Xing ◽  
Feng Chen ◽  
Liumin He ◽  
Kwok-Fai So ◽  
...  
Keyword(s):  
Human Liver ◽  
Early Stage ◽  
Three Dimensional ◽  
Alternative Methods ◽  
Liver Sinusoidal Endothelial Cells ◽  
Medicine Research ◽  
Cell Lineages ◽  
Self Assembled ◽  
Liver Tissues

The severe shortage of donor liver organs requires the development of alternative methods to provide transplantable liver tissues such as stem cell-derived organoids. Despite several studies describing the generation of vascularized and functional liver tissues, none have succeeded in assembling human liver buds containing hepatic stellate cells (HSCs) and liver sinusoidal endothelial cells (LSECs). Here, we report a reproducible, easy-to-follow, and comprehensive self-assembly protocol to generate three-dimensional (3D) human liver buds from naïve mesenchymal stem cells (MSCs), MSC-derived hepatocytes, and HSC- and LSEC-like cells. By optimizing the ratio between these different cell lineages, the cell mixture self-assembled into 3D human liver buds within 72 h in vitro, and exhibited similar characteristics with early-stage murine liver buds. In a murine model of acute liver failure, the mesenteric transplantation of self-assembled human liver buds effectively rescued animal death, and triggered hepatic ameliorative effects that were better than the ones observed after splenic transplantation of human hepatocytes or naïve MSCs. In addition, transplanted human liver buds underwent maturation during injury alleviation, after which they exhibited a gene expression profile signature similar to the one of adult human livers. Collectively, our protocol provides a promising new approach for the in vitro construction of functional 3D human liver buds from multiple human MSC-derived hepatic cell lineages; this new technique would be useful for clinical transplantation and regenerative medicine research.

scholarly journals The HNF1α-Regulated LncRNA HNF1α-AS1 Is Involved in the Regulation of Cytochrome P450 Expression in Human Liver Tissues and Huh7 Cells

2019 ◽  
Vol 368 (3) ◽  
pp. 353-362 ◽  
Author(s):  
Yiting Wang ◽  
Liang Yan ◽  
Jingyang Liu ◽  
Shitong Chen ◽  
Guangming Liu ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Human Liver ◽  
Huh7 Cells ◽  
P450 Expression ◽  
Liver Tissues

Researches on Liver Biopsy Assessment of Preliminary Data Obtained from the Study of Endogenous Respiration of Human Liver Tissues

2009 ◽  
Vol 161 (2) ◽  
pp. 117-124
Author(s):  
L. VILLA ◽  
L. NATALE ◽  
N. DIOGUARDI ◽  
L. CONTRO ◽  
G. C. SECCHI ◽  
...  
Keyword(s):  
Liver Biopsy ◽  
Preliminary Data ◽  
Human Liver ◽  
Endogenous Respiration ◽  
Liver Tissues

scholarly journals Hepatocarcinogenesis in FXR−/− Mice Mimics Human HCC Progression That Operates through HNF1α Regulation of FXR Expression

2012 ◽  
Vol 26 (5) ◽  
pp. 775-785 ◽  
Author(s):  
Nian Liu ◽  
Zhipeng Meng ◽  
Guiyu Lou ◽  
Weiping Zhou ◽  
Xiaoqiong Wang ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Human Liver ◽  
Gene Promoter ◽  
Normal Liver ◽  
Nuclear Hormone Receptor ◽  
Farnesoid X Receptor ◽  
Gene Profiling ◽  
Wild Type ◽  
Human Liver Cancer ◽  
Liver Tissues

Abstract Farnesoid X receptor (FXR) (nuclear receptor subfamily 1, group H, member 4) is a member of nuclear hormone receptor superfamily, which plays essential roles in metabolism of bile acids, lipid, and glucose. We previously showed spontaneously hepatocarcinogenesis in aged FXR−/− mice, but its relevance to human hepatocellular carcinoma (HCC) is unclear. Here, we report a systematical analysis of hepatocarcinogenesis in FXR−/− mice and FXR expression in human liver cancer. In this study, liver tissues obtained from FXR−/− and wild-type mice at different ages were compared by microarray gene profiling, histological staining, chemical analysis, and quantitative real-time PCR. Primary hepatic stellate cells and primary hepatocytes isolated from FXR−/− and wild-type mice were also analyzed and compared. The results showed that the altered genes in FXR−/− livers were mainly related to metabolism, inflammation, and fibrosis, which suggest that hepatocarcinogenesis in FXR−/− mice recapitulated the progression of human liver cancer. Indeed, FXR expression in human HCC was down-regulated compared with normal liver tissues. Furthermore, the proinflammatory cytokines, which were up-regulated in human HCC microenvironment, decreased FXR expression by inhibiting the transactivity of hepatic nuclear factor 1α on FXR gene promoter. Our study thereby demonstrates that the down-regulation of FXR has an important role in human hepatocarcinogenesis and FXR−/− mice provide a unique animal model for HCC study.

scholarly journals Editor’s Highlight: Modeling Compound-Induced FibrogenesisIn VitroUsing Three-Dimensional Bioprinted Human Liver Tissues

2016 ◽  
Vol 154 (2) ◽  
pp. 354-367 ◽  
Author(s):  
Leah M. Norona ◽  
Deborah G. Nguyen ◽  
David A. Gerber ◽  
Sharon C. Presnell ◽  
Edward L. LeCluyse
Keyword(s):  
Human Liver ◽  
Three Dimensional ◽  
Liver Tissues
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