scholarly journals Histological insight into the hepatic tissue of the Nile monitor (Varanus niloticus)

2018 ◽  
Vol 2 (3) ◽  
pp. 240-250
Author(s):  
Yasser A. Ahmed ◽  
Mohammed Abdelsabour Khalaf ◽  
Elsaysed Mohammed
Keyword(s):  
Light And Electron Microscopy ◽  
Lymphatic Vessels ◽  
Left Lobe ◽  
Hepatic Tissue ◽  
Histological Structure ◽  
Central Vein ◽  
Bile Canaliculi ◽  
Portal Area ◽  
Space Of Disse ◽  
Liver Fragments

The liver of reptiles is considered an important study model for the interaction between environment and hepatic tissue. Little is known about the histology of the liver of reptiles. The aim of the current study was to elucidate the histological architecture of the liver of the Nile monitor (Varanus niloticus). Liver fragments from the Nile monitor were collected in the summer season and processed for the light and electron microscopy. The liver of the Nile monitor was bi-lobed and the right lobe was found to be larger than the left lobe. Histological examination revealed indistinct lobulation of the liver, and the central vein, sinusoids and portal area were haphazardly organized. The hepatic parenchyma consisted of hepatocytes arranged in glandular-like alveoli or tubules separated by a network of twisted capillary sinusoids. The hepatocytes were polyhedral in shape with vacuolated cytoplasm and the nucleus was single rounded, eccentric, large and vesicular with a distinct nucleolus. The hepatocytes contained numerous lipid droplets, abundant glycogen granules and well-developed RER and mitochondria. The hepatocytes appeared to secrete into the bile canaliculi through the disintegration of their dark cytoplasm into the bile canaliculi. The space of Disse separating between the hepatocytes and sinusoids contained many recesses. The portal area contained branches of the portal vein, hepatic artery, bile duct and lymphatic vessels embedded in a connective tissue. Some non-parenchymal cells were described such as Kupffer cells, heterophils, melano-macrophages, intercalated cells, myofibroblasts in addition to the endothelium of the sinusoids. This is the first report about the histological structure of the liver of the Egyptian Nile monitor. The result presented here should be considered a baseline knowledge to compare with the pathological affections of the liver in this species.

scholarly journals Morpho-histological comparative study of the liver in White-eared bulbul (Pycnonotus leucotis), Mallard duck (Anas platyrhynchos), and Gull (Larus canus)

2018 ◽  
Vol 41 (2) ◽  
pp. 94-99
Author(s):  
Khalid Ibrahim Abd Al-Khazraji
Keyword(s):  
Food Environment ◽  
Left Lobe ◽  
Histological Structure ◽  
Central Vein ◽  
Mallard Duck ◽  
Hepatic Parenchyma ◽  
Anatomical Features ◽  
Right Lobe ◽  
Hepatic Portal ◽  
The Right

     This study was conducted to compare some of anatomical features and histological structure of the liver in three species of birds that varied in their size, taxonomy, and food environment. The study revealed that the liver in the mallard, gull and white-eared bulbul was bilobed big gland, and the left lobe was smaller than the right one. It was observed that the two lobes in mallard were undivided, while the right lobe in gull has two parts, whereas the left lobe in white-eared bulbul is subdivided into two parts. Histologically, the liver capsule in gull appeared thicker than other two species. The hepatic parenchyma was not shown to be clearly defined lobules in three species. The hepatocytes were organized radially around the central vein as plates or cords of one-two cell thick in mallard and gull, and of two-several cell thick in white-eared bulbul, and those plates were separated by blood sinusoids. It turned out that the hepatic portal triads were less numerous in white-eared bulbul compared with other two species. It was found that there are differences in measurements of some histological structures of the liver among the three species.

scholarly journals Review on the Lymphatic Vessels in the Dental Pulp

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1257
Author(s):  
Kamila Wiśniewska ◽  
Zbigniew Rybak ◽  
Maria Szymonowicz ◽  
Piotr Kuropka ◽  
Maciej Dobrzyński
Keyword(s):  
Dental Pulp ◽  
Lymphatic System ◽  
Light And Electron Microscopy ◽  
Fluid Pressure ◽  
Lymphatic Vessels ◽  
Main Role ◽  
Reaction Products ◽  
Dental Tissues ◽  
Optical Electron ◽  
Antigen Antibody

Despite many studies, opinions on the lymphatic system of the teeth are still incompatible. Studies using light and electron microscopy and directly using methods such as a radioisotope (radionuclide) scan and interstitial fluid pressure measurement reported incomplete results. Immunohistochemistry (IHC) plays the main role in investigating presence of the lymphatic system in dental tissues. This method uses labeled antibodies against antigens typical of lymphatic vessels. The use of appropriate staining enables the detection of antigen-antibody reaction products using a light (optical), electron or fluorescence microscope. However, these studies do not show the system of vessels, their histologic structure under physiological conditions and inflammation as well as the lymphangiogenesis process in the dental pulp. Unfortunately, there is a lack of studies associating the presence of lymphatic vessels in the dental pulp with local lymphatic nodes or large vessels outside the tooth. In the scientific and research environment, the evaluation of the lymphatic system of the teeth is problematic because it is quite difficult to clearly distinguish lymphatic vessels from small blood vessels. Despite many indications of the presence of lymphatic vessels in the pulp chamber, this problem remains open and needs further research.

scholarly journals Effect of Aspirin drug in Liver tissue of swiss white mice Mus musculus

2014 ◽  
Vol 11 (3) ◽  
pp. 1089-1093
Author(s):  
Baghdad Science Journal
Keyword(s):  
Acetylsalicylic Acid ◽  
Mus Musculus ◽  
Liver Tissue ◽  
Control Group ◽  
Histological Structure ◽  
Central Vein ◽  
Liver Necrosis ◽  
Hepatic Cells ◽  
Negative Effect ◽  
Radial Arrangement

This study was designed to identify the extent of the effect of drug Acetylsalicylic acid (aspirin) on histological structure of liver in Swiss albino mice Mus musculus.(16) mice were used and divided into two groups. The first groupwas orally treated with )0.5ml(of aspirinat concentration of (50 mcg / kg) and the second group was considered as control group Treated with distiled water lasted for 30 days. Treatment with Aspirin led to the occurrence of histopathological cases included liver necrosis ,cellular infilitration, congestion in central vein and a case of Hemorrhage. The results also showed the absence of radial arrangement of the constituents of hepatic cells from tissue due to these changes with the control group. From the above results, it coulde be concluded that the drug aspirin had a negative effect on liver of Swiss albino

Demonstration of Noradrenaline-Immunoreactive Nerve Fibres in the Liver

1996 ◽  
Vol 24 (6) ◽  
pp. 466-472 ◽  
Author(s):  
Y Fukuda ◽  
M Imoto ◽  
Y Koyama ◽  
Y Miyazawa ◽  
T Hayakawa
Keyword(s):  
Human Liver ◽  
Central Vein ◽  
Nerve Fibres ◽  
Portal Area ◽  
Cacodylate Buffer ◽  
Antibody Method ◽  
Tissue Specimens ◽  
Cryostat Sections ◽  
Distribution Of Noradrenaline ◽  
Liver Tissues

To demonstrate noradrenaline-immunoreactive nerve fibres in liver tissues, we used an antibody to noradrenaline in the immunostaining of liver tissues from rats, guinea-pigs and humans. The tissue specimens were fixed by perfusion or immersion with cacodylate buffer containing sodium metabisulphate and glutaraldehyde, and cryostat sections were prepared. An indirect peroxidase-labelled antibody method was used for staining noradrenaline. Noradrenaline-immunoreactive nerve fibres were localized around blood vessels in the portal area and around the central vein. There were differences between the species in the intralobular distribution of noradrenaline-immunoreactive fibres. Normal guinea-pig and human liver showed intralobular noradrenaline-immunoreactive fibres while rat liver did not. Noradrenaline-immunoreactive fibres were absent from regenerating nodules in a human cirrhotic liver. This method of demonstrating noradrenaline directly using perfusion- or immersion-fixation is appropriate for studying innervation in normal and damaged livers of various species including humans.

scholarly journals Morphological and histological study of the liver in migratory starling bird (Sturnus vulgaris)

2017 ◽  
Vol 27 (5) ◽  
pp. 11
Author(s):  
Saddama Saed Faraj ◽  
Genan Adnan Al-Bairuty
Keyword(s):  
Abdominal Cavity ◽  
Liver Parenchyma ◽  
Sturnus Vulgaris ◽  
Histological Structure ◽  
Central Vein ◽  
Connective Tissue Capsule ◽  
Histochemical Observation ◽  
Pas Staining ◽  
Right Lobe ◽  
Tissue Capsule

The present work was aimed to form the baseline data of normal morphological and histological structure features of liver in migratory starling (Sturnus vulgaris). Anatomically, the starling liver bird was dark red -brown in colour and located in the cranial third of the abdominal cavity and consisted of undivided lobes (left and right). The liver right lobe was larger than the left. Histological examination revealed that the liver parenchyma was covered by a connective tissue capsule which appears to be thicker in the rim of liver lobes than other area in the liver lobe. Liver parenchyma was arranged in an unlimited hepatic lobules, which composed of polygonal hepatocytes organized as irregular, radial interconnecting cords or laminae of one or two cells thickness around a central vein and separated by blood sinusoids. In the boundary of each lobule showed a portal area which consists of a branch of hepatic artery; one or more branches of hepatic vein and one to four branches of the bile duct which lining by cuboidal cells that characterized by their empty non-staining cytoplasm. The histochemical observation by using PAS staining in the current study revealed that the glycogen granules arranged close to the central vein and in the rim of liver lobules.The present work was aimed to form the baseline data of normal morphological and histological structure features of liver in migratory starling (Sturnus vulgaris). Anatomically, the starling liver bird was dark red -brown in colour and located in the cranial third of the abdominal cavity and consisted of undivided lobes (left and right). The liver right lobe was larger than the left. Histological examination revealed that the liver parenchyma was covered by a connective tissue capsule which appears to be thicker in the rim of liver lobes than other area in the liver lobe. Liver parenchyma was arranged in an unlimited hepatic lobules, which composed of polygonal hepatocytes organized as irregular, radial interconnecting cords or laminae of one or two cells thickness around a central vein and separated by blood sinusoids. In the boundary of each lobule showed a portal area which consists of a branch of hepatic artery; one or more branches of hepatic vein and one to four branches of the bile duct which lining by cuboidal cells that characterized by their empty non-staining cytoplasm. The histochemical observation by using PAS staining in the current study revealed that the glycogen granules arranged close to the central vein and in the rim of liver lobules. Keywords: Birds, Liver morphology, liver histology.

scholarly journals Expression of a homologously recombined erythopoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells

Blood ◽  
1994 ◽  
Vol 84 (6) ◽  
pp. 1823-1830 ◽  
Author(s):  
PH Maxwell ◽  
DJ Ferguson ◽  
MK Osmond ◽  
CW Pugh ◽  
A Heryet ◽  
...  
Keyword(s):  
Fusion Gene ◽  
Light And Electron Microscopy ◽  
T Antigen ◽  
Cell Populations ◽  
Cell Type ◽  
Sv40 T Antigen ◽  
Ito Cells ◽  
Ultrastructural Characterization ◽  
Space Of Disse

We have obtained transgenic mice in which an erythropoietin-SV40 virus T antigen fusion gene is homologously recombined into the native Epo locus. This gene is expressed in a tissue-specific manner closely resembling that of the native Epo gene. Immunohistochemical detection of SV40 T antigen has been used to characterize the hepatic cell populations expressing the transgene. In mice stimulated by anaemia or hypobaric hypoxia, SV40 T antigen was demonstrated in two liver cell populations: a subset of hepatocytes and a nonparenchymal cell type. Immunohistochemical and ultrastructural characterization of these cells by light and electron microscopy showed the nonparenchymal cell type to be the Ito cells, which lie in a persinusoidal position within the space of Disse. We therefore conclude that Ito cells are the nonhepatocytic source of liver Epo production. These cells show many similarities to the Epo-producing fibroblastoid interstitial cells of the kidney.

The Fine Structure of Brunner’s Glands in the Human

1967 ◽  
Vol 25 ◽  
pp. 66-67
Author(s):  
T. S. Lesson ◽  
C. R. Leeson
Keyword(s):  
Light And Electron Microscopy ◽  
Histological Structure ◽  
Surgical Biopsy ◽  
Brunner's Glands ◽  
Biopsy Specimens ◽  
Brunner’S Glands ◽  
Submucosal Glands ◽  
History Of ◽  
The Common ◽  
Foamy Cytoplasm

Duodenal submucosal glands occur in all mammals but show species differences both in their extent and histological structure. Surgical biopsy specimens were obtained from five individuals aged from 37 to 56 with no history of duodenal abnormality. In all cases the patient was starved for four to eight hours prior to operation and the biopsies were taken from the second part of the duodenum immediately proximal to the opening of the common bile duct. The tissue was prepared both for light and electron microscopy.By light microscopy Brunner’s glands in the human appear as masses of coiled tubules in the submucosa with ducts opening into the bases of intestinal glands. Component cells are characteristically mucous in type with dark, irregular, basally-located nuclei and clear, foamy cytoplasm.

Changes in Expression of Bile Canalicular CD10 and Sinusoidal CD105 (Endoglin) in Peritumoral Hepatic Tissue

2009 ◽  
Vol 95 (4) ◽  
pp. 495-500 ◽  
Author(s):  
Toshitsugu Nakamura
Keyword(s):  
Hepatocellular Carcinoma ◽  
Chronic Hepatitis ◽  
Tumor Growth ◽  
Expression Patterns ◽  
Metastatic Carcinoma ◽  
Hepatic Tissue ◽  
Bile Canaliculi ◽  
Metastatic Tumors ◽  
Phenotypic Changes ◽  
Resected Liver

Aims and background Hepatic tissues, including bile canaliculi and sinusoids, around primary or metastatic tumors are destructed and regenerate associated with tumor growth, and may show some phenotypic changes. The present study was undertaken to examine the expression of CD10 in bile canaliculi [CD10(BC)] and CD105 (endoglin) along hepatic sinusoids [CD105(HS)] in peritumoral hepatic tissue (PTH). Methods Fifty samples of resected liver bearing hepatocellular carcinoma (HCC) or metastatic carcinoma were immunostained for CD10 and CD105. The immunoreactivity for CD10(BC) and CD105(HS) in the background hepatic tissue of tumors and PTH was scored separately. Results CD10(BC) was moderately or markedly expressed in the background hepatic tissue without chronic hepatitis or cirrhosis in most of the cases, and was significantly downregulated in chronic hepatitis and cirrhosis. CD105(HS) was negative or minimally positive in most of the cases of hepatic tissue bearing metastatic carcinoma, and showed a significant increase in chronic hepatitis and cirrhosis. Compared with the background, PTH revealed significantly decreased CD10(BC) staining irrespective of HCC or metastatic carcinoma, and showed belt-like CD105(HS) expression in 66.7% of the cases of metastatic carcinoma and in 88.6% of those with HCC. Conclusions These data indicate that the expression patterns of CD10(BC) and CD105(HS) in PTH are similar to those in chronic hepatitis and cirrhosis, which may be caused by persistent injury and resultant regeneration of hepatic tissue.

Sign in / Sign up

Export Citation Format

Share Document