IgA Antibodies Against Gliadin and Tissue Transglutaminase in Dogs With Chronic Enteritis and Intestinal T-Cell Lymphoma

Molecular clonality analysis of T-cell receptor (TCR) genes for diagnosing T-cell lymphoma is widely used in veterinary medicine. However, differentiating chronic enteritis (CE) from intestinal lymphoma is challenging because of the incompatibility between histopathologic and clonality analysis results. On the basis of findings that canine intestinal T-cell lymphoma and celiac disease share some common features, we conducted serologic examinations in combination with histopathologic and T-cell receptor clonality analyses in 48 dogs diagnosed with either CE or intestinal lymphoma. Immunoglobulin A (IgA) and immunoglobulin G (IgG) antibodies against gliadin and tissue transglutaminase (tTG) were quantitatively measured using ELISA. The conditions were classified according to the histopathologic diagnosis, clonality analysis, and combined histopathologic/clonality analysis. Histopathologic analysis showed that dogs with intestinal lymphoma were likely to have high levels of serum IgA antibodies against gliadin and tTG, and serum IgG antibodies against tTG. No correlation between the diagnosed groups and control group was observed in the results of the clonality analysis and histopathologic/clonality analysis. It is interesting that dogs with intestinal lymphoma had a higher serum IgA titer against gliadin and tTG than did dogs with CE. These results suggest an association between repetitive inflammatory stimulation by gliadin peptides and subsequent intestinal lymphoma in dogs.

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Characterization of the humoral immune response to the EBV proteome in extranodal NK/T-cell lymphoma

Scientific Reports ◽

10.1038/s41598-021-02788-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Zhiwei Liu ◽

Yomani D. Sarathkumara ◽

John K. C. Chan ◽

Yok-Lam Kwong ◽

Tai Hing Lam ◽

...

Keyword(s):

Immune Response ◽

T Cell ◽

Antibody Response ◽

Cell Lymphoma ◽

T Cell Lymphoma ◽

Igg Antibodies ◽

Humoral Immune ◽

Epithelial Origin ◽

Iga Antibodies ◽

Associated Tumors

AbstractExtranodal natural killer/T-cell lymphoma (NKTCL) is an aggressive malignancy that has been etiologically linked to Epstein-Barr virus (EBV) infection, with EBV gene transcripts identified in almost all cases. However, the humoral immune response to EBV in NKTCL patients has not been well characterized. We examined the antibody response to EBV in plasma samples from 51 NKTCL cases and 154 controls from Hong Kong and Taiwan who were part of the multi-center, hospital-based AsiaLymph case–control study. The EBV-directed serological response was characterized using a protein microarray that measured IgG and IgA antibodies against 202 protein sequences representing the entire EBV proteome. We analyzed 157 IgG antibodies and 127 IgA antibodies that fulfilled quality control requirements. Associations between EBV serology and NKTCL status were disproportionately observed for IgG rather than IgA antibodies. Nine anti-EBV IgG responses were significantly elevated in NKTCL cases compared with controls and had ORshighest vs. lowest tertile > 6.0 (Bonferroni-corrected P-values < 0.05). Among these nine elevated IgG responses in NKTCL patients, three IgG antibodies (all targeting EBNA3A) are novel and have not been observed for other EBV-associated tumors of B-cell or epithelial origin. IgG antibodies against EBNA1, which have consistently been elevated in other EBV-associated tumors, were not elevated in NKTCL cases. We characterize the antibody response against EBV for patients with NKTCL and identify IgG antibody responses against six distinct EBV proteins. Our findings suggest distinct serologic patterns of this NK/T-cell lymphoma compared with other EBV-associated tumors of B-cell or epithelial origin.

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Anaplastic Large T-Cell Lymphoma in the Intestine of Dogs

Veterinary Pathology ◽

10.1177/0300985819852132 ◽

2019 ◽

Vol 56 (6) ◽

pp. 878-884 ◽

Cited By ~ 1

Author(s):

Lauren W. Stranahan ◽

Derick Whitley ◽

Tuddow Thaiwong ◽

Matti Kiupel ◽

Fabiano Oliveira

Keyword(s):

Gastrointestinal Tract ◽

T Cell ◽

Intestinal Perforation ◽

Cell Lymphoma ◽

Cell Receptor ◽

T Cell Lymphoma ◽

Intestinal Lymphoma ◽

Treatment Protocols ◽

Selection Of

Anaplastic large T-cell lymphoma (ALTCL) is a rare subtype of non-Hodgkin T-cell lymphoma that occasionally occurs in the gastrointestinal tract of humans. Enteropathy-associated T-cell lymphoma (EATL) type 1 is the most common type of intestinal lymphoma in dogs, and ALTCL has not previously been reported in the intestinal tract of dogs. Thirteen dogs with intestinal masses diagnosed as intestinal lymphoma with anaplastic morphology were reviewed. Clinical data, including treatment protocols, were available for 11 cases. Immunohistochemistry for CD3, CD20, and CD30 was performed for all cases in addition to PCR for Antigen Receptor Rearrangements (PARR) for assessment of clonality. Eight (62%) of the cases presented with intestinal perforation, and all cases had 1 or more masses arising from the small intestine. Histologically, all cases were characterized by transmural infiltrates of large, CD3-positive and frequently CD30-positive cells. Neoplastic T cells had marked anisocytosis and anisokaryosis, prominent nucleoli, and occasionally indented to reniform nuclei. There was abundant necrosis and inflammation with occasional vascular invasion within neoplastic masses. All cases had a monoclonal T-cell receptor γ gene rearrangement. The median survival time was 5 days, with 1 dog surviving 2 years after the initial diagnosis. ALTCL can occur as an aggressive transmural lymphoma in the gastrointestinal tract of dogs and commonly causes intestinal perforation. ALTCL can be differentiated from EATL type 1 and might have implications for accurate prognostication and selection of therapeutic options in the future.

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Clonality Analysis by T-Cell Receptor γ PCR and High-Resolution Electrophoresis in the Diagnosis of Cutaneous T-Cell Lymphoma (CTCL)

Cancer Cell Signaling ◽

10.1385/1-59259-356-9:303 ◽

2003 ◽

pp. 303-320

Author(s):

Ansgar Lukowsky

Keyword(s):

T Cell ◽

High Resolution ◽

T Cell Receptor ◽

Cell Lymphoma ◽

Cell Receptor ◽

T Cell Lymphoma ◽

Cutaneous T Cell Lymphoma ◽

Clonality Analysis

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Fusion transcripts FYN-TRAF3IP2 and KHDRBS1-LCK hijack T cell receptor signaling in peripheral T-cell lymphoma, not otherwise specified

Nature Communications ◽

10.1038/s41467-021-24037-4 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Koen Debackere ◽

Lukas Marcelis ◽

Sofie Demeyer ◽

Marlies Vanden Bempt ◽

Nicole Mentens ◽

...

Keyword(s):

T Cell ◽

T Cell Receptor ◽

Cell Lymphoma ◽

Ex Vivo ◽

Cell Receptor ◽

T Cell Lymphoma ◽

Peripheral T Cell Lymphoma ◽

Fusion Transcripts ◽

Not Otherwise Specified

AbstractPeripheral T-cell lymphoma (PTCL) is a heterogeneous group of non-Hodgkin lymphomas with poor prognosis. Up to 30% of PTCL lack distinctive features and are classified as PTCL, not otherwise specified (PTCL-NOS). To further improve our understanding of the genetic landscape and biology of PTCL-NOS, we perform RNA-sequencing of 18 cases and validate results in an independent cohort of 37 PTCL cases. We identify FYN-TRAF3IP2, KHDRBS1-LCK and SIN3A-FOXO1 as new in-frame fusion transcripts, with FYN-TRAF3IP2 as a recurrent fusion detected in 8 of 55 cases. Using ex vivo and in vivo experiments, we demonstrate that FYN-TRAF3IP2 and KHDRBS1-LCK activate signaling pathways downstream of the T cell receptor (TCR) complex and confer therapeutic vulnerability to clinically available drugs.

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Extranodal NK/T-cell lymphoma, nasal type presenting as primary intestinal lymphoma vs intestinal T-cell lymphoma: A borderline diagnostic category in the revised WHO classification

Human Pathology Case Reports ◽

10.1016/j.ehpc.2021.200534 ◽

2021 ◽

Vol 25 ◽

pp. 200534

Author(s):

Brady E. Beltrán ◽

Mario L. Marques-Piubelli ◽

M. Pilar Quiñones ◽

Esther Cotrina ◽

Eugenio A. Palomino ◽

...

Keyword(s):

T Cell ◽

Who Classification ◽

Cell Lymphoma ◽

Diagnostic Category ◽

T Cell Lymphoma ◽

Intestinal Lymphoma ◽

Nasal Type ◽

Nk T Cell

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PS1308 TARGETING T-CELL RECEPTOR SIGNALING PATHWAY BY DASATINIB IN RELAPSED/REFRACTORY ANGIOIMMUNOBLASTIC T-CELL LYMPHOMA

HemaSphere ◽

10.1097/01.hs9.0000563512.46613.6f ◽

2019 ◽

Vol 3 (S1) ◽

pp. 597

Author(s):

Y. Kiyoki ◽

M. Sakata-Yanagimoto ◽

T.B. Nguyen ◽

M. Fujisawa ◽

Y. Nannya ◽

...

Keyword(s):

T Cell ◽

Signaling Pathway ◽

T Cell Receptor ◽

Cell Lymphoma ◽

Cell Receptor ◽

T Cell Lymphoma ◽

Angioimmunoblastic T Cell Lymphoma ◽

T Cell Receptor Signaling ◽

Receptor Signaling Pathway ◽

Cell Receptor Signaling

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Diagnosis of T-cell lymphoma using beta F1, anti-T-cell receptor beta chain antibody

Histopathology ◽

10.1111/j.1365-2559.1989.tb03074.x ◽

1989 ◽

Vol 15 (3) ◽

pp. 239-247 ◽

Cited By ~ 8

Author(s):

A. S. Krajewski ◽

M. W. Myskow ◽

D. M. Salter ◽

D. S. Cunningham ◽

E. F. Ramage

Keyword(s):

T Cell ◽

T Cell Receptor ◽

Cell Lymphoma ◽

Cell Receptor ◽

T Cell Lymphoma ◽

Beta Chain ◽

T Cell Receptor Beta ◽

Receptor Beta

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Angioimmunoblastic T-Cell Lymphoma With Cutaneous Involvement: A Case Report With Subtle Histologic Changes and Clonal T-Cell Proliferation

Archives of Pathology & Laboratory Medicine ◽

10.5858/2004-128-e122-atlwci ◽

2004 ◽

Vol 128 (10) ◽

pp. e122-e124

Author(s):

Chien-Tai Huang ◽

Shih-Sung Chuang

Keyword(s):

T Cell ◽

T Cell Receptor ◽

Gene Rearrangement ◽

Cell Lymphoma ◽

Paraffin Section ◽

Cell Receptor ◽

T Cell Lymphoma ◽

Chain Gene ◽

Angioimmunoblastic T Cell Lymphoma ◽

Lymphoid Infiltrate

Abstract Angioimmunoblastic T-cell lymphoma is a nodal peripheral T-cell lymphoma that rarely involves the skin. We describe a 62-year-old Taiwanese man who developed a second relapse of angioimmunoblastic T-cell lymphoma with generalized erythroderma and numerous plaquelike and nodular lesions. Biopsy of the erythematous skin lesion demonstrated mild infiltrate of atypical small lymphocytes, some with clear cytoplasm. The lymphoid infiltrate was located mainly around skin appendages and in the upper dermis without epidermotropism. Immunohistochemically, these atypical lymphocytes expressed CD3. Polymerase chain reaction analysis for T-cell receptor γ-chain gene rearrangement using paraffin section showed the same-sized monoclonal bands in the skin and 2 previous nodal biopsies. We conclude that the histologic features of angioimmunoblastic T-cell lymphoma involving skin may be very subtle, showing only mild lymphoid infiltrate. Awareness of the history of angioimmunoblastic T-cell lymphoma with ancillary studies, including clonality testing for T-cell receptor gene rearrangement, is crucial for reaching an accurate diagnosis.

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