scholarly journals Nanog Expression and Proliferation Indices in Canine Cutaneous Mast Cell Tumors

2018 ◽  
Vol 55 (6) ◽  
pp. 849-852
Author(s):  
Julia A. Joselevitch ◽  
Camila N. Barra ◽  
Thiago Henrique M. Vargas ◽  
Lidia H. Pulz ◽  
Adriana T. Nishiya ◽  
...  
Keyword(s):  
Mast Cell ◽  
Histological Grade ◽  
Clinical Information ◽  
Ki67 Index ◽  
Mast Cell Tumors ◽  
Proliferation Indices ◽  
Nanog Expression ◽  
Mast Cell Tumor ◽  
Wide Range ◽  
Cutaneous Mast Cell

Mast cell tumors are one of the most frequent skin tumors in dogs. Treatment decisions often depend on a wide range of clinical information and the main criteria for prognostic formulation are histological grade, mitotic count, Ki67 index, and KIT immunostaining pattern. NANOG is a pluripotency factor expressed by normal and cancer stem cells, which is a prognostic marker and a potential therapeutic target for several human tumors. In the present study, mast cell tumor samples from 41 dogs were evaluated for NANOG and Ki67 by immunohistochemistry. All samples were positive for NANOG but its expression was not correlated with Ki67 index and no significant differences were found with respect to histopathological grades, disease-related mortality, or survival. Our results suggest that, although related to pluripotency, NANOG expression does not correlate with proliferative activity, and is not a reliable prognostic factor for canine cutaneous mast cell tumors.

scholarly journals Stereology in Grading and Prognosis of Canine Cutaneous Mast Cell Tumors

2021 ◽  
pp. 030098582098513
Author(s):  
Mafalda Casanova ◽  
Sandra Branco ◽  
Inês Berenguer Veiga ◽  
André Barros ◽  
Pedro Faísca
Keyword(s):  
Mast Cell ◽  
Clinical Outcome ◽  
Prognostic Value ◽  
Histological Grade ◽  
Intraobserver Variability ◽  
Low Grade ◽  
High Grade ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell ◽  
Grade Iii

Canine cutaneous mast cell tumors (ccMCTs) are currently graded according to Patnaik and Kiupel grading schemes. The qualitative and semiquantitative parameters applied in these schemes may lead to inter- and intraobserver variability. This study investigates the prognostic value of volume-weighted mean nuclear volume ([Formula: see text]), a stereological estimation that provides information about nuclear size and its variability. [Formula: see text] of 55 ccMCTs was estimated using the “point-sampled intercept” method and compared with histological grade and clinical outcome. The clinical history of dogs treated with surgical excision alone was available for 30 ccMCTs. Statistical differences in [Formula: see text] were found between grade II ([Formula: see text]= 115 ± 29 µm3) and grade III ccMCTs ([Formula: see text]= 197 ± 63 µm3), as well as between low-grade ([Formula: see text]= 113 ± 28 µm3) and high-grade ccMCTs ([Formula: see text]= 184 ± 63 µm3). An optimal cutoff value of [Formula: see text] ≥ 150 µm3 and [Formula: see text] ≥ 140 µm3 was determined for grade III and high-grade ccMCTs, respectively. In terms of prognosis, [Formula: see text] was not able to predict the clinical outcome in 42% of the cases; however, cases with [Formula: see text]<125 µm3 had a favorable outcome. These results indicate that, despite having limited prognostic value when used as a solitary parameter, [Formula: see text] is highly reproducible and is associated with histological grade as well as with benign behavior.

scholarly journals Morphometry of Canine Cutaneous Mast Cell Tumors

2003 ◽  
Vol 40 (3) ◽  
pp. 268-275 ◽  
Author(s):  
R. De F. Strefezzi ◽  
J. G. Xavier ◽  
J. L. Catão-Dias
Keyword(s):  
Mast Cell ◽  
Needle Aspiration ◽  
Nuclear Area ◽  
Mast Cell Tumors ◽  
Mast Cell Tumor ◽  
Mean Diameter ◽  
Poorly Differentiated ◽  
Cutaneous Mast Cell ◽  
Histopathologic Analysis ◽  
Well Differentiated

Twenty-four canine cutaneous nodules, diagnosed as mast cell tumors by fine-needle aspiration biopsy and confirmed by histopathologic analysis by staining with hematoxylin and eosin (HE) and toluidine blue, were analyzed by computerized nuclear morphometry on panoptic- and HE-stained cytopathology slides. Two hundred nuclei per lesion were examined. The morphometric parameters investigated were nuclear area, mean diameter, perimeter, regularity factor, and ellipticity factor. Lesions were graded as I (well differentiated), II (intermediate differentiation), or III (poorly differentiated) according to the following morphologic features: invasiveness, cellularity and cellular morphology, mitotic index, and stromal reaction. Nuclear morphometric results were then compared with histopathologic grades. Values of nuclear area, mean diameter, and perimeter increased with increase in histopathologic grade, but statistical analysis revealed significant differences only between grades II and III and between grades I and III when HE was used ( P, 0.01) and between grades I and III with panoptic stain ( P, 0.05). The ellipticity factor and regularity factor did not reveal significant differences between histopathologic grades. The results indicate that nuclear morphometric analysis, in combination with the rapid and inexpensive cytopathology technique, can help in mast cell tumor grading, thus contributing to the establishment of a more precise prognosis and treatment.

scholarly journals Kit Receptor Expression in Canine Cutaneous Mast Cell Tumors (CMCTs) Without C-Kit Mutation

2016 ◽  
Vol 66 (2) ◽  
pp. 222-233
Author(s):  
Ivana Vučićević ◽  
Darko Marinković ◽  
Vladimir Kukolj ◽  
Miloš Vučićević ◽  
Milorad Mirilović ◽  
...  
Keyword(s):  
Mast Cell ◽  
Histopathological Examination ◽  
Polymerase Chain Reaction Amplification ◽  
Histological Grade ◽  
Receptor Expression ◽  
Tissue Samples ◽  
Kit Mutation ◽  
Mast Cell Tumors ◽  
Kit Receptor ◽  
Cutaneous Mast Cell

Abstract Histopathological examination, grading, immunohistochemical staining and molecular genetic examinations are the proposed criteria that should be used for cutaneous mast cell tumors (CMCTs) classification. The presence of aberrant CD117 expression and mutations of the c-kit proto-oncogene could be an indicative parameter for final histological grading. Determination of the connection between the localization of KIT receptor expression and the histological grade of CMCTs without c-kit proto-oncogene mutations was the main goal of this study. The study included twenty four CMCTs and six control skin samples from 30 dogs of different ages, breed and sex. Formalinfixed and paraffin-embedded tissue samples were stained with hematoxylin-eosin and toluidine blue and immunohistochemically tested for CD117 expression. DNA was extracted from the same paraffin blocks and subsequent polymerase chain reaction amplification was performed using PE1 and PE2 primers. Degree of malignancy was determined based on the presence of mitotic figures, multinucleated cells, bizarre nuclei and karyomegaly in 10 high power fields. Based on histological features, fourteen of 24 CMCTs were of a high histological grade, while ten were classified as a lowgrade malignancy. CD117 cytoplasmic expression was observed in nine of fourteen high-grade malignancy CMCTs, which confirms the link between the aberrant CD117 expression and increased cell proliferation.

Radiation Therapy for Incompletely Excised Grade II Canine Mast Cell Tumors

2006 ◽  
Vol 42 (6) ◽  
pp. 430-434 ◽  
Author(s):  
Valerie Jasmine Poirier ◽  
William M. Adams ◽  
Lisa J. Forrest ◽  
Eric M. Green ◽  
Richard R. Dubielzig ◽  
...  
Keyword(s):  
Mast Cell ◽  
Lymph Node ◽  
Regional Lymph Node ◽  
Overall Survival Rate ◽  
Mast Cell Tumors ◽  
Mast Cell Tumor ◽  
Grade Ii ◽  
Cutaneous Mast Cell ◽  
Lymph Node Irradiation ◽  
Prophylactic Irradiation

Forty-five dogs with incompletely excised grade II mast cell tumors were treated with radiation using a cobalt 60 teletherapy unit (15 fractions of 3.2 Gy for a total of 48 Gy). Twenty-four of the dogs underwent prophylactic regional lymph node irradiation. Three (6.7%) dogs had tumor recurrence, two (4.4%) dogs developed metastasis, and 14 (31%) dogs developed a second cutaneous mast cell tumor. No difference in overall survival rate was observed between the dogs receiving and not receiving prophylactic irradiation of the regional lymph node.

Extracutaneous Mast-Cell Tumor in the Dog

1982 ◽  
Vol 19 (6) ◽  
pp. 608-615 ◽  
Author(s):  
A. K. Patnaik ◽  
E. G. MacEwen ◽  
A. P. Black ◽  
S. Luckow
Keyword(s):  
Duodenal Ulcer ◽  
Mast Cell ◽  
Lymph Nodes ◽  
Cell Tumor ◽  
Regional Lymph Nodes ◽  
Duodenal Ulcers ◽  
Mast Cell Tumors ◽  
Mast Cell Tumor ◽  
Cutaneous Mast Cell ◽  
Limited Metastasis

Three neoplasms of extracutaneous mast-cell origin, arising from the nasopharynx, oral cavity, and hepatopancreatic lymph nodes respectively, were diagnosed in three dogs. The neoplasms had histologic features similar to those of cutaneous mast-cell tumors, but had limited metastasis mostly involving the regional lymph nodes. One dog had a perforating duodenal ulcer, suggesting that duodenal ulcers can occur with extracutaneous tumors as they do with some cutaneous mast-cell tumors in the dog.

scholarly journals HSP32 and HSP90 Immunoexpression, in Relation to Kit Pattern, Grading, and Mitotic Count in Canine Cutaneous Mast Cell Tumors

2016 ◽  
Vol 54 (2) ◽  
pp. 222-225 ◽  
Author(s):  
M. Romanucci ◽  
M. Massimini ◽  
A. Ciccarelli ◽  
D. Malatesta ◽  
L. Bongiovanni ◽  
...  
Keyword(s):  
Mast Cell ◽  
Histological Grade ◽  
Tumor Grade ◽  
Mitotic Count ◽  
Immunohistochemical Expression ◽  
Mast Cell Tumors ◽  
Great Heterogeneity ◽  
Poorly Differentiated ◽  
Cutaneous Mast Cell

Literature data indicate heat shock protein (Hsp) 32 and 90 as potential molecular targets in canine neoplastic mast cells (MCs). However, their immunoexpression patterns in canine mast cell tumors (MCTs) have not been investigated. Thus, the aim of this study was to evaluate the immunohistochemical expression of Hsp32 and Hsp90 in 22 canine cutaneous MCTs, in relation to KIT immunolabeling pattern, histological grade, and mitotic count. All cases showed cytoplasmic labeling of Hsp90, variably associated with nuclear and/or membranous labeling. Relationships of Hsp90 or Hsp32 immunolabeling with KIT pattern, mitotic count, and tumor grade were not observed. However, the reduced Hsp32 immunoexpression observed in most grade III/high-grade MCTs suggests a tendency toward a loss of immunosignal in poorly differentiated MCs. The great heterogeneity in extent and distribution of Hsp90 immunoexpression among the different MCT cases may also partially explain the difficulties in predicting the in vivo biologic activity of Hsp90 inhibitors on canine MCTs.

scholarly journals RETRACTED ARTICLE: Cutaneous mast cell tumor (Mastocytoma): Cyto- histopathological and haematological investigations

2014 ◽  
Vol 9 (1) ◽  
Author(s):  
Ehsan Hosseini ◽  
Behnam Pedram ◽  
Ali Mohammad Bahrami ◽  
Mohammad Hossein Jaberi Moghaddam ◽  
Javad Javanbakht ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Tumor ◽  
Mast Cell Tumor ◽  
Retracted Article ◽  
Cutaneous Mast Cell

scholarly journals Ancillary techniques on the evaluation of canine cutaneous mast cell tumors from Brazil

2016 ◽  
Vol 46 (10) ◽  
pp. 1804-1810 ◽  
Author(s):  
Mariana Martins Flores ◽  
Renata Dalcol Mazaro ◽  
Ingeborg Maria Langohr ◽  
Alma Roy ◽  
Keith Strother ◽  
...  
Keyword(s):  
Mast Cell ◽  
Pcr Amplification ◽  
The United States ◽  
Growth Fraction ◽  
Grading System ◽  
Low Grade ◽  
Ki 67 ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell ◽  
Exon 11

ABSTRACT: The use of histologic classification by a 2-tier grading system only, immunohistochemistry (IHC) for KIT and Ki-67 and polymerase chain reaction (PCR) for internal tandem duplications (ITD) on exon 11 has improved the prognostication of canine cutaneous mast cell tumors (CCMTs) particularly in the United States. However, these techniques are not commonly used in most Brazilian laboratories. Likewise, no studies, to date, have investigated the occurrence of ITD in CCMTs from the country. Thus, this study tested the 2-tier grading system, the immunohistochemistry for KIT and Ki-67 and the PCR for exon 11 in a group of Brazilian CCMTs with the goal of investigating the applicability of these tests in a Brazilian laboratory. Of the 39 CCMTs, 69.2% (27/39) were identified as low-grade and 30.8% (12/39) as high-grade by a 2-tier grading system. All tumors had a KIT expression pattern II, and 30.6% (11/36) had a high growth fraction (Ki-67). PCR amplification was successful in four of the 11 tumors examined. Two of these (50%) were positive for ITD. This study highlights the importance of using auxiliary techniques in the CCMT evaluation, identifies limitations and confirms the applicability of these methods on a routine diagnostic basis in Brazil. Our results will help to improve the prognostication of CCMTs in Brazilian diagnostic laboratories, encouraging the use of supplementary methods.

scholarly journals Retraction note: Cutaneous mast cell tumor (Mastocytoma): cyto-histopathological and haematological investigations

2016 ◽  
Vol 11 (1) ◽  
Author(s):  
Ehsan Hosseini ◽  
Behnam Pedram ◽  
Ali Mohammad Bahrami ◽  
Mohammad Hossein Jaberi Moghaddam ◽  
Javad Javanbakht ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Tumor ◽  
Mast Cell Tumor ◽  
Cutaneous Mast Cell
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