NOS Evaluations in Human Dental Pulp-Capping with MTA and Calcium-Hydroxide

2007 ◽  
Vol 20 (1_suppl) ◽  
pp. 27-32 ◽  
Author(s):  
C. D'arcangelo ◽  
F. Di Nardo-Di Maio ◽  
C. Patrono ◽  
S. Caputi
Keyword(s):  
Calcium Hydroxide ◽  
Dental Pulp ◽  
Pulp Capping ◽  
Dental Pulp Capping ◽  
Human Dental Pulp

scholarly journals Materiais Utilizados para Capeamento Pulpar Direto: uma Revisão da Literatura

2018 ◽  
Vol 19 (5) ◽  
pp. 209
Author(s):  
C M A Silva ◽  
L S Paiva ◽  
D V Oliveira ◽  
B O Nobre ◽  
T A D Mendes ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Dental Pulp ◽  
Biocompatible Materials ◽  
Pulp Capping ◽  
Dental Pulp Capping

O objetivo deste estudo é avaliar as propriedades físico-químicas e biológicas dos principais materiais indicados para capeamento pulpar direto. Revisou-se a literatura, nos bancos de dados Pubmed, Scopus e Scielo, entre 2007 e 2017, buscando artigos em português e em inglês, utilizando os descritores combinados: “Dental Pulp Capping”, “Biocompatible Materials” e “Calcium Hydroxide” como estratégia de busca. Foram obtidos 55 artigos em que 21 estavam em repetição e foram excluídos, dos demais 6 eram revisão de literatura, 3 eram ensaios clínicos e 25 estudos laboratoriais. Através de uma leitura crítica dos títulos e dos resumos, foram selecionados 10 artigos segundo sua relevância para o estudo. Os materiais mais relatados foram o hidróxido de cálcio, o agregado trióxido mineral (MTA) e o silicato tricálcio (biodentine®). O primeiro contém propriedades antibacterianas e biocompatibilidade, entretanto possui a sua alta solubilidade como desvantagem. O segundo apresenta resultados mais previsíveis que o primeiro na formação da barreira dentinária, melhor biocompatibilidade e boa capacidade seladora. O terceiro mostra maior formação de dentina terciária, promovendo um bom selamento marginal e mostra a menor solubilidade entre eles. Assim, os materiais MTA e biodentine mostram-se materiais viáveis ao tradicional hidróxido de cálcio.Palavras-chave: Dental Pulp Capping. Biocompatible Materials. Calcium Hydroxide. 

scholarly journals Evaluation of Resin-Based Material Containing Copaiba Oleoresin (Copaifera Reticulata Ducke): Biological Effects on the Human Dental Pulp Stem Cells

Biomolecules ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 972
Author(s):  
Roberta Souza D’Almeida Couto ◽  
Maria Fernanda Setubal Destro Rodrigues ◽  
Leila Soares Ferreira ◽  
Ivana Márcia Alves Diniz ◽  
Fernando de Sá Silva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Biological Effects ◽  
Dental Pulp Stem Cells ◽  
Nodule Formation ◽  
Alizarin Red ◽  
Pulp Capping ◽  
Hsp 27 ◽  
Dental Pulp Capping ◽  
Human Dental Pulp

The purpose of this study was to analyze in vitro the biological effects on human dental pulp stem cells triggered in response to substances leached or dissolved from two experimental cements for dental pulp capping. The experimental materials, based on extracts from Copaifera reticulata Ducke (COP), were compared to calcium hydroxide [Ca(OH)2] and mineral trioxide aggregate (MTA), materials commonly used for direct dental pulp capping in restorative dentistry. For this, human dental pulp stem cells were exposed to COP associated or not with Ca(OH)2 or MTA. Cell cytocompatibility, migration, and differentiation (mineralized nodule formation (Alizarin red assay) and gene expression (RT-qPCR) of OCN, DSPP, and HSP-27 (genes regulated in biomineralization events)) were evaluated. The results showed that the association of COP reduced the cytotoxicity of Ca(OH)2. Upregulations of the OCN, DSPP, and HSP-27 genes were observed in response to the association of COP to MTA, and the DSPP and HSP-27 genes were upregulated in the Ca(OH)2 + COP group. In up to 24 h, cell migration was significantly enhanced in the MTA + COP and Ca(OH)2 + COP groups. In conclusion, the combination of COP with the currently used materials for dental pulp capping [Ca(OH)2 and MTA] improved the cell activities related to pulp repair (i.e., cytocompatibility, differentiation, mineralization, and migration) including a protective effect against the cytotoxicity of Ca(OH)2.

scholarly journals Cytotoxicity and Bioactivity of Dental Pulp-Capping Agents towards Human Tooth-Pulp Cells: A Systematic Review of In-Vitro Studies and Meta-Analysis of Randomized and Controlled Clinical Trials

Materials ◽  
2020 ◽  
Vol 13 (12) ◽  
pp. 2670 ◽  
Author(s):  
Mariano S. Pedano ◽  
Xin Li ◽  
Kumiko Yoshihara ◽  
Kirsten Van Landuyt ◽  
Bart Van Meerbeek
Keyword(s):  
Calcium Hydroxide ◽  
Calcium Silicate ◽  
Dental Pulp ◽  
Meta Analysis ◽  
Capping Agents ◽  
Human Dental Pulp Cells ◽  
Pulp Capping ◽  
Human Dental Pulp ◽  
Pulp Cells

Background. In the era of biology-driven endodontics, vital pulp therapies are regaining popularity as a valid clinical option to postpone root-canal treatment. In this sense, many different materials are available in the market for pulp-capping purposes. Objectives. The main aim of this systematic review and meta-analysis was to examine literature regarding cytotoxicity and bioactivity of pulp-capping agents by exposure of human dental pulp cells of primary origin to these materials. A secondary objective was to evaluate the inflammatory reaction and reparative dentin-bridge formation induced by the different pulp-capping agents on human pulp tissue. Data sources. A literature search strategy was carried out on PubMed, EMBASE and the Web of Science databases. The last search was done on 1 May 2020. No filters or language restrictions were initially applied. Two researchers independently selected the studies and extracted the data. Study selection included eligibility criteria, participants and interventions, study appraisal and synthesis methods. In vitro studies were included when human dental pulp cells of primary origin were (in) directly exposed to pulp-capping agents. Parallel or split-mouth randomized or controlled clinical trials (RCT or CCT) were selected to investigate the effects of different pulp-capping agents on the inflammation and reparative bridge-formation capacity of human pulp tissue. Data were synthesized via odds ratios (95% confidence interval) with fixed or random effects models, depending on the homogeneity of the studies. The relative risks (95% confidence interval) were presented for the sake of interpretation. Results. In total, 26 in vitro and 30 in vivo studies were included in the systematic review and meta-analysis, respectively. The qualitative analysis of in vitro data suggested that resin-free hydraulic calcium-silicate cements promote cell viability and bioactivity towards human dental pulp cells better than resin-based calcium-silicate cements, glass ionomers and calcium-hydroxide cements. The meta-analysis of the in vivo studies indicated that calcium-hydroxide powder/saline promotes reparative bridge formation better than the popular commercial resin-free calcium-silicate cement Pro-Root MTA (Dentsply-Sirona), although the difference was borderline non-significant (p = 0.06), and better than calcium-hydroxide cements (p < 0.0001). Moreover, resin-free pulp-capping agents fostered the formation of a complete reparative bridge better than resin-based materials (p < 0.001). On the other hand, no difference was found among the different materials tested regarding the inflammatory effect provoked at human pulp tissue. Conclusions. Calcium-hydroxide (CH) powder and Pro-Root MTA (Dentsply-Sirona) have shown excellent biocompatibility in vitro and in vivo when tested on human cells and teeth. Their use after many years of research and clinical experience seems safe and proven for vital pulp therapy in healthy individuals, given that an aseptic environment (rubber dam isolation) is provided. Although in vitro evidence suggests that most modern hydraulic calcium-silicate cements promote bioactivity when exposed to human dental pulp cells, care should be taken when these new materials are clinically applied in patients, as small changes in their composition might have big consequences on their clinical efficacy. Key findings (clinical significance). Pure calcium-hydroxide powder/saline and the commercial resin-free hydraulic calcium-silicate cement Pro-Root MTA (Dentsply-Sirona) are the best options to provide a complete reparative bridge upon vital pulp therapy. Systematic review registration number. PROSPERO registration number: CRD42020164374.

scholarly journals Dental Stem Cell Therapy with Calcium Hydroxide in Dental Pulp Capping

2010 ◽  
Vol 16 (6) ◽  
pp. 1823-1833 ◽  
Author(s):  
Young-Min Ji ◽  
Soung Hoo Jeon ◽  
Joo-Young Park ◽  
Jong-Hoon Chung ◽  
Yun-Hoon Choung ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Therapy ◽  
Calcium Hydroxide ◽  
Stem Cell Therapy ◽  
Dental Pulp ◽  
Pulp Capping ◽  
Dental Pulp Capping ◽  
Dental Stem Cell

scholarly journals Comparison of Four Dental Pulp-Capping Agents by Cone-Beam Computed Tomography and Histological Techniques—A Split-Mouth Design Ex Vivo Study

2021 ◽  
Vol 11 (7) ◽  
pp. 3045
Author(s):  
Jayanandan Muruganandhan ◽  
Govindarajan Sujatha ◽  
Saravanan Poorni ◽  
Manali Ramakrishnan Srinivasan ◽  
Nezar Boreak ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Calcium Hydroxide ◽  
Cone Beam Computed Tomography ◽  
Dental Pulp ◽  
Cone Beam ◽  
Capping Agents ◽  
Bridge Formation ◽  
Pulp Capping ◽  
Dental Pulp Capping ◽  
Post Hoc

Dental pulp-capping is done to preserve vital teeth when the pulp is exposed due to caries, trauma or instrumentation. Various materials are used as pulp-capping agents. The introduction of newer materials requires scientific studies to assess their clinical efficacy. The study was designed as a split-mouth randomized analysis of four pulp-capping agents (calcium hydroxide, mineral trioxide aggregate (MTA), Biodentine and EndoSequence root repair material (ERRM)). Based on selection criteria, 15 orthodontic patients requiring the extraction of four premolars (60 teeth total) were included in the study. After pulp-capping, the teeth were extracted after 8 weeks. We analyzed the extracted teeth using cone-beam computed tomography (CBCT) and histological sections to determine the quality of the dentinal bridge and the pulpal response. Ordinal scores were given based on the completeness of the dentinal bridge, the type of bridge and the degree of pulpal inflammation. Results were analyzed using a Kruskal–Wallis test (p < 0.05) with post hoc Conover values being used when applicable. All four pulp-capping materials elicited dentinal bridge formation (60/60). MTA had the highest scores (10/15) in dentinal bridge formation followed by ERRM (8/15). Both materials showed more samples with complete dentinal bridges (9/15 each) and a favorable pulpal response (15/15). Teeth capped with calcium hydroxide showed more cases of incomplete bridge formation (9/15) and pulpal inflammation. These differences in dentinal bridge formation and pulpal inflammation were statistically significant (p 0.001 and p 0.00005, respectively), with post hoc tests revealing no significant differences between MTA and ERRM (p 0.49 and p 0.71, respectively). MTA and ERRM performed better than the other pulp-capping materials but did not differ significantly from each other. The individual preference for a pulp-capping material may be based on clinical efficacy and handling characteristics.

scholarly journals Characterization of Chlorhexidine-Loaded Calcium-Hydroxide Microparticles as a Potential Dental Pulp-Capping Material

2017 ◽  
Vol 4 (4) ◽  
pp. 59 ◽  
Author(s):  
Balasankar Priyadarshini ◽  
Subramanian Selvan ◽  
Karthikeyan Narayanan ◽  
Amr Fawzy
Keyword(s):  
Calcium Hydroxide ◽  
Dental Pulp ◽  
Pulp Capping ◽  
Dental Pulp Capping

scholarly journals Avaliação da Adesão, Sorção de Água e Solubilidade de Resinas Compostas Autoadesivas Experimentais para Capeamento Pulpar Direto

2018 ◽  
Vol 19 (5) ◽  
pp. 102
Author(s):  
J C Silva ◽  
A E C Ferreira-Júnior ◽  
P G B Silva ◽  
A P N Nunes Alves ◽  
M R L Mota ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Dental Pulp ◽  
Pulp Capping ◽  
Dental Pulp Capping ◽  
Push Out

O objetivo foi analisar resinas compostas experimentais para capeamento pulpar direto em propriedades físico-químicas selecionadas, comparando com hidróxido de cálcio fotopolimerizável e MTA. Duas resinas compostas capeadoras foram preparadas, uma sem adição de partículas bioativas (controle) e a outra com 30% em peso (experimental) de nanopartículas de hidroxiapatita adsorvidas com sinvastatina (10%), com glutationa (10%) e de beta fosfato tricálcio (10%). Foram utilizados 36 terceiros molares hígidos extraídos, onde foi realizado preparo cavitário na região coronária até atingir a câmara pulpar para posterior restauração com um dos materiais. Os espécimes foram cortados em fatias (1mm) e essas foram submetidas ao teste de resistência de união ao push-out. Espécimes em forma de disco (n8) foram preparados e avaliados (ISO4049) quanto a sorção e solubilidade. A análise estatística  foi feita com ANOVA e teste de Tukey (a5%). Os resultados de resistência de união mostraram que o MTA e o hidróxido de cálcio apresentaram pior adesão do que as resinas, e os maiores valores foram obtidos com a resina experimental (p<0,05). Maior sorção de água foi observada com o hidróxido de cálcio e os demais materiais não apresentaram diferenças estatísticas. O hidróxido de cálcio obteve maior solubilidade que a resina controle e a experimental (p<0,05). Em conclusão, a resina experimental com partículas bioativas promove maior adesão à dentina e menor sorção e solubilidade que hidróxido de cálcio e MTA, esses materiais que são tradicionalmente usados para capeamento pulpar.Palavras-chave: Dental Pulp Capping. Simvastatin Calcium Hydroxide.

scholarly journals Human dental pulp stem cell responses to different dental pulp capping materials

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Chawan Manaspon ◽  
Chavin Jongwannasiri ◽  
Sujin Chumprasert ◽  
Noppadol Sa-Ard-Iam ◽  
Rangsini Mahanonda ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Marker Gene ◽  
Serum Free Medium ◽  
Free Medium ◽  
Alizarin Red ◽  
Serum Free ◽  
Pulp Capping ◽  
Dental Pulp Capping ◽  
Human Dental Pulp

Abstract Background Direct pulp capping is a vital pulp therapy for a pin-point dental pulp exposure. Applying a pulp capping material leads to the formation of a dentin bridge and protects pulp vitality. The aim of this study was to compare the effects of four dental materials, DyCal®, ProRoot® MTA, Biodentine™, and TheraCal™ LC in vitro. Methods Human dental pulp stem cells (hDPs) were isolated and characterized. Extraction medium was prepared from the different pulp capping materials. The hDP cytotoxicity, proliferation, and migration were examined. The odonto/osteogenic differentiation was determined by alkaline phosphatase, Von Kossa, and alizarin red s staining. Osteogenic marker gene expression was evaluated using real-time polymerase chain reaction. Results ProRoot® MTA and Biodentine™ generated less cytotoxicity than DyCal® and TheraCal™ LC, which were highly toxic. The hDPs proliferated when cultured with the ProRoot® MTA and Biodentine™ extraction media. The ProRoot® MTA and Biodentine™ extraction medium induced greater cell attachment and spreading. Moreover, the hDPs cultured in the ProRoot® MTA or Biodentine™ extraction medium migrated in a similar manner to those in serum-free medium, while a marked reduction in cell migration was observed in the cells cultured in DyCal® and TheraCal™ LC extraction media. Improved mineralization was detected in hDPs maintained in ProRoot® MTA or Biodentine™ extraction medium compared with those in serum-free medium. Conclusion This study demonstrates the favorable in vitro biocompatibility and bioactive properties of ProRoot® MTA and Biodentine™ on hDPs, suggesting their superior regenerative potential compared with DyCal® and TheraCal™.

Sign in / Sign up

Export Citation Format

Share Document