A laboratory study to test the responses of human dental pulp stem cells to extracts from three dental pulp capping biomaterials

The purpose of this study was to analyze in vitro the biological effects on human dental pulp stem cells triggered in response to substances leached or dissolved from two experimental cements for dental pulp capping. The experimental materials, based on extracts from Copaifera reticulata Ducke (COP), were compared to calcium hydroxide [Ca(OH)2] and mineral trioxide aggregate (MTA), materials commonly used for direct dental pulp capping in restorative dentistry. For this, human dental pulp stem cells were exposed to COP associated or not with Ca(OH)2 or MTA. Cell cytocompatibility, migration, and differentiation (mineralized nodule formation (Alizarin red assay) and gene expression (RT-qPCR) of OCN, DSPP, and HSP-27 (genes regulated in biomineralization events)) were evaluated. The results showed that the association of COP reduced the cytotoxicity of Ca(OH)2. Upregulations of the OCN, DSPP, and HSP-27 genes were observed in response to the association of COP to MTA, and the DSPP and HSP-27 genes were upregulated in the Ca(OH)2 + COP group. In up to 24 h, cell migration was significantly enhanced in the MTA + COP and Ca(OH)2 + COP groups. In conclusion, the combination of COP with the currently used materials for dental pulp capping [Ca(OH)2 and MTA] improved the cell activities related to pulp repair (i.e., cytocompatibility, differentiation, mineralization, and migration) including a protective effect against the cytotoxicity of Ca(OH)2.

Download Full-text

Effect of an Experimental Direct Pulp-capping Material on the Properties and Osteogenic Differentiation of Human Dental Pulp Stem Cells

Scientific Reports ◽

10.1038/srep34713 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 15

Author(s):

Fan Yu ◽

Yan Dong ◽

Yan-wei Yang ◽

Ping-ting Lin ◽

Hao-han Yu ◽

...

Keyword(s):

Stem Cells ◽

Osteogenic Differentiation ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Direct Pulp Capping ◽

Pulp Capping ◽

Human Dental Pulp

Download Full-text

Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells

Odontology ◽

10.1007/s10266-020-00571-3 ◽

2020 ◽

Author(s):

Sinem Kuru ◽

Elif Sepet ◽

Tülay İrez ◽

Esin Aktaş ◽

Yusufhan Yazır ◽

...

Keyword(s):

Stem Cells ◽

Cell Death ◽

Signaling Pathways ◽

Dental Pulp ◽

Lipoteichoic Acid ◽

Dental Pulp Stem Cells ◽

Pulp Capping ◽

Human Dental Pulp ◽

Cell Death Signaling

Download Full-text

Effects of two fast-setting pulp-capping materials on cell viability and osteogenic differentiation in human dental pulp stem cells: An in vitro study

Archives of Oral Biology ◽

10.1016/j.archoralbio.2019.02.014 ◽

2019 ◽

Vol 100 ◽

pp. 100-105 ◽

Cited By ~ 1

Author(s):

Yan Sun ◽

Jun Liu ◽

Tao Luo ◽

Ya Shen ◽

Ling Zou

Keyword(s):

Stem Cells ◽

Cell Viability ◽

Osteogenic Differentiation ◽

Dental Pulp ◽

In Vitro Study ◽

Dental Pulp Stem Cells ◽

Vitro Study ◽

Pulp Capping ◽

Human Dental Pulp

Download Full-text

The Biomineralization of a Bioactive Glass-Incorporated Light-Curable Pulp Capping Material Using Human Dental Pulp Stem Cells

BioMed Research International ◽

10.1155/2017/2495282 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 24

Author(s):

Soo-Kyung Jun ◽

Jung-Hwan Lee ◽

Hae-Hyoung Lee

Keyword(s):

Stem Cells ◽

Bioactive Glass ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Similar Amount ◽

Alizarin Red ◽

Ion Concentrations ◽

Pulp Capping ◽

Alp Activity ◽

Human Dental Pulp

The aim of this study was to investigate the biomineralization of a newly introduced bioactive glass-incorporated light-curable pulp capping material using human dental pulp stem cells (hDPSCs). The product (Bioactive® [BA]) was compared with a conventional calcium hydroxide-incorporated (Dycal [DC]) and a light-curable (Theracal® [TC]) counterpart. Eluates from set specimens were used for investigating the cytotoxicity and biomineralization ability, determined by alkaline phosphatase (ALP) activity and alizarin red staining (ARS). Cations and hydroxide ions in the extracts were measured. An hDPSC viability of less than 70% was observed with 50% diluted extract in all groups and with 25% diluted extract in the DC. Culturing with 12.5% diluted BA extract statistically lowered ALP activity and biomineralization compared to DC (p<0.05), but TC did not (p>0.05). Ca (~110 ppm) and hydroxide ions (pH 11) were only detected in DC and TC. Ionic supplement-added BA, which contained similar ion concentrations as TC, showed similar ARS mineralization compared to TC. In conclusion, the BA was similar to, yet more cytotoxic to hDPSCs than, its DC and TC. The BA was considered to stimulate biomineralization similar to DC and TC only when it released a similar amount of Ca and hydroxide ions.

Download Full-text

Biocompatibility and Bioactivity of Set Direct Pulp Capping Materials on Human Dental Pulp Stem Cells

Materials ◽

10.3390/ma13183925 ◽

2020 ◽

Vol 13 (18) ◽

pp. 3925

Author(s):

Yemi Kim ◽

Donghee Lee ◽

Dani Song ◽

Hye-Min Kim ◽

Sin-Young Kim

Keyword(s):

Stem Cells ◽

Cell Migration ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Nodule Formation ◽

Alizarin Red ◽

Pulp Capping ◽

Alp Activity ◽

Human Dental Pulp ◽

And Control

In this study, we assessed the biocompatibility and bioactivity of various pulp capping materials—ProRoot MTA (Dentsply Tulsa Dental Specialties), Biodentine (Septodont), TheraCal LC (Bisco), and Dycal (Dentsply Caulk)—on human dental pulp stem cells (hDPSCs). Experimental disks (diameter, 7 mm; height, 4 mm) were stored in a humified incubator at 37 °C for 48 h. Then, the pulp capping materials were tested for cytotoxic effects by methyl-thiazoldiphenyl-tetrazolium and scratch wound healing assays, and for mineralization potential by Alizarin red S (ARS) staining assay and alkaline phosphatase enzyme (ALP) activity. Cell viability and cell migration did not significantly differ between ProRoot MTA, Biodentine, and control (p > 0.05). TheraCal LC exhibited slower cell migration on days 2–4 compared to control (p < 0.05), and Dycal showed no cell migration. ALP activity was highest with Biodentine on days 10 and 14, and was lowered with TheraCal LC and Dycal (p < 0.05). In the ARS assay, hDPSCs grown in ProRoot MTA and TheraCal LC eluates showed significantly increased mineralized nodule formation on day 21 compared to Biodentine, Dycal, and control (p < 0.05). These findings indicate that ProRoot MTA, Biodentine, and TheraCal LC exhibit better biocompatibility and bioactivity than Dycal.

Download Full-text