Effects of extremely low-frequency electromagnetic field exposure on the skeletal muscle functions in rats

2020 ◽  
Vol 36 (2) ◽  
pp. 119-131
Author(s):  
Sevgi Gunes ◽  
Belgin Buyukakilli ◽  
Selma Yaman ◽  
Cagatay Han Turkseven ◽  
Ebru Ballı ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Muscle Tissue ◽  
Chronic Exposure ◽  
Low Frequency ◽  
Diaphragm Muscle ◽  
Mechanical Activity ◽  
Control Group ◽  
Female Group ◽  
Bioelectrical Activity ◽  
Extremely Low Frequency

The aim of the present study was to systematically investigate the effects of chronic exposure to extremely low-frequency electromagnetic field (ELF-EMF) on electrophysiological, histological and biochemical properties of the diaphragm muscle in rats. Twenty-nine newly weaned (24 days old, 23–80 g) female ( n = 15) and male ( n = 14) Wistar Albino rats were used in this study. The animals were randomly divided into two groups: the control group and the electromagnetic field (EMF) group. The control group was also randomly divided into two groups: the control female group and the control male group. The EMF exposure group was also randomly divided into two groups: the ELF-EMF female group and the ELF-EMF male group. The rats in the ELF-EMF groups were exposed for 4 h daily for up to 7 months to 50 Hz frequency, 1.5 mT magnetic flux density. Under these experimental conditions, electrophysiological parameters (muscle bioelectrical activity parameters: intracellular action potential and resting membrane potential and muscle mechanical activity parameter: force–frequency relationship), biochemical parameters (Na+, K+, Cl− and Ca+2 levels in the blood serum of rats; Na+-K+ ATPase enzyme-specific activities in muscle tissue; and free radical metabolism in both muscle tissue and serum) and transmission electron microscopic morphometric parameters of the diaphragm muscle were determined. We found that chronic exposure to ELF-EMF had no significant effect on the histological structure and mechanical activity of the muscle and on the majority of muscle bioelectrical activity parameters, with the exception of some parameters of muscle bioelectrical activity. However, the changes in some bioelectrical activity parameters were relatively small and unlikely to be clinically relevant.

DETOXICATION PROPERTIES OF COMBINED FOOD ORGANIC FIBERS OBTAINED FROM SECONDARY RAW MATERIALS OF SUGAR BEET PRODUCTION

2019 ◽  
Author(s):  
М.Ю. ТАМОВА ◽  
Е.В. БАРАШКИНА ◽  
Р.А. ЖУРАВЛЕВ ◽  
Н.Р. ТРЕТЬЯКОВА ◽  
А.Х. ШАНТЫЗ ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Citric Acid ◽  
Dietary Fiber ◽  
Low Frequency ◽  
Lead Content ◽  
Control Group ◽  
Dietary Fibers ◽  
Extremely Low Frequency ◽  
White Rats ◽  
Beet Pulp

Проведена медикобиологическая оценка детоксикационных свойств комбинированных пищевых волокон (КПВ) растительного происхождения, полученных по разработанной авторами технологии. В качестве объекта исследования были КПВ из свекловичного жома: после экстракции лимонной кислотой образец 1 и после обработки ЭМП КНЧ образец 2. Для определения детоксикационной активности КПВ сформировано 3 группы беспородных белых крыс по 20 голов в каждой, которые вместе с основным кормом получали свинец в виде свинцовой соли уксусной кислоты из расчета 25,0 мг свинца/кг массы тела. 1я и 2я опытные группы получали к корму добавку КПВ в количестве 0,6 мг/кг массы тела животного. Исследования проводили в течение 62 сут. По завершении эксперимента установлено, что у крыс 1й и 2й опытных групп, получавших КПВ из свекловичного жома, прирост живой массы тела превышал аналогичный показатель контрольной группы на 32,7 и 35,8 соответственно содержание свинца в крови снизилось относительно контрольной группы на 36,3 и 43,8 соответственно содержание свинца в печени соответственно в 1,8 и 2 раза ниже по сравнению с контрольной группой животных. Установлено, что детоксикационная активность КПВ после обработки ЭМП КНЧ выше, чем после экстракции лимонной кислотой. Biomedical assessment of detoxification properties of combined dietary fiber of plant origin, obtained by the technology developed by the authors, was carried out. Combined dietary fiber from beet pulp: after extraction with citric acid sample 1 and after treatment with an extremely low frequency electromagnetic field sample 2 were the object of study. To determine the detoxification activity of combined dietary fibers 3 groups of mongrel white rats with 20 heads in each were formed. All animals in the main feed added lead in the form of lead of salt acetic acid from calculation 25,0 mg lead/kg masses body rats. Combined dietary fibers were added to the animal feed of the 1st and 2nd experimental groups in the amount of 0,6 mg/kg of animal body weight. The studies were carried out for 62 days. At the end of the experiment, it was found that in rats of the 1st and 2nd experimental groups, where combined beet pulp fibers were added to the main food, the live weight gain exceeded the same indicator of the control group by 32,7 and 35,8, respectively the lead content in the blood decreased relative to the control group by 36,3 and 43,8, respectively the lead content in the liver, respectively, was 1,8 and 2 times lower compared to the control group of animals. It was found that the detoxification activity of combined dietary fibers after treatment with an electromagnetic field of extremely low frequency is higher than after extraction with citric acid.

scholarly journals The Parietal Eye of Lizards (Pogona vitticeps) Needs Light at a Wavelength Lower than 580 nm to Activate Light-Dependent Magnetoreception

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 489
Author(s):  
Tsutomu Nishimura
Keyword(s):  
Electromagnetic Field ◽  
Electric Fields ◽  
Low Frequency ◽  
Control Group ◽  
Average Increase ◽  
Extremely Low Frequency ◽  
The Third ◽  
Average Ratio ◽  
Pogona Vitticeps ◽  
Parietal Eye

In a previous study, the agamid lizard Pogona vitticeps was discovered to respond to an electromagnetic field (EMF) of extremely low frequency (6 and 8 Hz; peak magnetic and electric fields of 2.6 µT and 10 V/m, respectively). Furthermore, when the third eye of a lizard was covered, using a small round aluminum cap, the reaction to the EMF disappeared. These results suggested that the parietal eye has a role in light-dependent magnetoreception. However, the wavelength of light needed to activate light-dependent magnetoreception has not been identified and was thus explored in the present study. Lizards were randomly divided into control and EMF groups. In both groups, a small round light-absorbing filter was positioned on the back of the head of each lizard and blocked light of wavelengths lower than 580 nm. The EMF group was subjected to EMF exposure for half of the day, whereas the control group was not. No significant intergroup differences were discovered in the average ratio of the number of tail lifts on test days to the baseline value or average increase in the number of test-day tail lifts minus the baseline value (p = 0.41 and p = 0.67, respectively). Lizards with light-absorption filters that cut out light with wavelengths lower than 380 nm were found to respond to the EMF. Therefore, the lizards appeared to respond to light of certain wavelengths rather than the filters themselves. The results of these experiments suggest that light of wavelengths lower than 580 nm is required to activate light-dependent magnetoreception in the parietal eye of P. vitticeps.

Extremely low-frequency electromagnetic field (EMF) generates alterations in the synthesis and secretion of oestradiol-17 β (E 2 ) in uterine tissues: An in vitro study

2018 ◽  
Vol 110 ◽  
pp. 86-95 ◽  
Author(s):  
Anna Koziorowska ◽  
Ewa Monika Waszkiewicz ◽  
Maria Romerowicz-Misielak ◽  
Kamila Zglejc-Waszak ◽  
Anita Franczak
Keyword(s):  
Electromagnetic Field ◽  
Low Frequency ◽  
In Vitro Study ◽  
Vitro Study ◽  
Extremely Low Frequency

Extremely low-frequency electromagnetic field induces neural differentiation of hBM-MSCs through regulation of (Zn)-metallothionein-3

2017 ◽  
Vol 38 (5) ◽  
pp. 364-373 ◽  
Author(s):  
Anastasia Rosebud Aikins ◽  
Sung-Won Hong ◽  
Hyun-Jung Kim ◽  
Cheol-Ho Yoon ◽  
Joo-Hee Chung ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Neural Differentiation ◽  
Low Frequency ◽  
Extremely Low Frequency

scholarly journals Investigation of the Effect of Sodium Selenate on Acetylcholinesterase Activity under Extremely Low Frequency Electromagnetic Field

2012 ◽  
Vol 4 (1) ◽  
Author(s):  
Ezzatollah Fathi ◽  
Raheleh Farahzadi
Keyword(s):  
Electromagnetic Field ◽  
Structural Changes ◽  
Tertiary Structure ◽  
Low Frequency ◽  
Acetylcholinesterase Activity ◽  
The Elderly ◽  
Skin Aging ◽  
Sodium Selenate ◽  
Spectroscopic Techniques ◽  
Extremely Low Frequency

Acetylcholinestrase (AChE EC 3.1.1.7) is one of the most important enzymes in nervous system, which plays a role in Alzheimer’s disease. Selenium is a vital micronutrient and many investigations have been performed about the physiological, biochemical and behavioral effects of this element, such as postponing the Alzheimer's symptoms in the elderly and delaying the initiation signs of skin aging. Recent studies have shown that this element protects various enzymes against the toxicity caused by heavy metals such as; Pb, Al, Cu and Cd. AChE activity is altered under the influence of extremely low frequency electromagnetic field (ELF-EMF). In this study, the effects of ELF-EMF, with 0.3 mT field intensity and 50, 100, 217 Hz frequencies, were investigated on the AChE, in the presence of different concentrations of sodium selenate, using UV-Visible, fluorescence and circular dichroism spectroscopic techniques. The results demonstrated that the enzyme activity declined by increasing the frequency and the amount of sodium selenate. Also, significant structural changes occurred in the secondary and tertiary structures of AChE. Our results showed that with increasing the concentration of sodium selenate transition from α-helix to β-structure was appeared in the presence of ELF-EMF. In conclusion, according to changes observed in the secondary and tertiary structure of enzyme, it is proposed that these fields are able to affect the structure and dynamics of the active site gorge of AChE.

scholarly journals The Effect of 12 Hz Extremely Low-Frequency Electromagnetic Fields on Visual Memory of Male Macaque Monkeys

2021 ◽  
Author(s):  
Masoomeh Kazemi ◽  
◽  
Hamed Aliyari ◽  
Elaheh Tekieh ◽  
Hassan Tavacoli ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Visual Memory ◽  
Rhesus Macaques ◽  
Low Frequency ◽  
Serum Levels ◽  
Control Group ◽  
Behavioral Tests ◽  
Extremely Low Frequency ◽  
Before And After ◽  
Male Rhesus

Introduction: Today, humans leave in a world surrounded by electromagnetic fields. Numerous studies have been carried out to discover the biological, physiological, and behavioral effects of electromagnetic fields on humans and animals. Given the biological similarities between monkeys and humans, the goal of the present research was to examine Visual Memory (VM), hormonal, genomic, and anatomic changes, and changes of the amygdala function in the male rhesus macaques who exposed to extremely low-frequency magnetic fields (ELF/MF). Materials and methods: Four male Rhesus Macaques (Macaca mulatta) were used. For the behavioral tests the animals were needed to be fasting for 17 hours, and for the behavioral tests such as visual memory, cooperation of the animal was necessary. Using the radiation protocol, two of the monkeys were exposed to 12-Hz electromagnetic fields with a magnitude of 0.7 microtesla (electromagnetic radiation) four hours a day for a month. Before and after the exposure, a visual memory test was conducted using a coated device (visible reward) on a movable stand. Ten mL of blood was obtained from the femoral artery of the monkeys and half of it was used to examine cortisol serum levels using MyBioSource kits (made in the USA). The other half of blood samples were used to extract lymphocytes for assaying expressions of Glucocorticoid Receptor (GR) genes before and after radiation using the PCR method. Anatomic studies of the amygdala were carried out based on pre- and post-radiation Magnetic Resonance Imaging (MRI). Findings: Research results indicated that visual memory in male primates increased significantly at the 12Hz frequency. Hormonal analysis at the 12Hz frequency showed a decrease in cortisol serum levels. However, visual memory and serum cortisol levels did not change considerably in male primates in the control group. There was no considerable amygdala volumetric difference at the 12 Hz frequency. The expression of the GR genes decreased at 12Hz compared to the control group. Conclusion: In short, these results indicated that ELF may have a beneficial value for memory enhancement as indicated by the fact that exposure to the 12 HZ ELF can enhance visual memory. This may be due to a decrease in plasma cortisol, and/or expression of GR genes. Moreover, direct involvement of the amygdala in this regard cannot be recommended.

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