Chemopreventive effect of galangin against benzo(a)pyrene-induced stomach tumorigenesis through modulating aryl hydrocarbon receptor in Swiss albino mice

The present study was aimed to evaluate the chemopreventive potential of galangin against benzo(a)pyrene (BaP)-induced stomach carcinogenesis in Swiss albino mice. Stomach cancer was induced in experimental mice using BaP oral administration. The mice were treated with galangin (10 mg/kg b.wt.) before and during BaP administration. Oral administration of galangin at a dose of 10 mg/kg b.wt. significantly (p < 0.05) prevented the tumor incidence, tumor volume in the experimental animals. Further, galangin pretreatment prevents BaP-induced lipid peroxidation and restores BaP-mediated loss of cellular antioxidants status. It has also been found that galangin prevents BaP-induced activation of phase I detoxification enzymes. Furthermore, galangin pretreatment prevented the BaP-induced overexpression of cytochrome P450s isoform genes (CYP1A1, CYP1B1), aryl hydrocarbon receptor system (AhR, ARNT), transcriptional activators (CBP/p300, NF-kB), tumor growth factors, proto-oncogenes, invasion markers (TGFB, SRC-1, MYC, iNOS, MMP2, MMP9) and Phase II metabolic isoenzyme genes (GST) in the stomach tissue homogenate when compared to the control groups. The western blot results confirm that galangin (10 mg/kg. b.wt.) treatment significantly prevented the BaP-mediated expression of ArR, ARNT, and CYP1A1 proteins in the mouse stomach tissue. Therefore, the present results confirm that galangin prevents BaP-induced stomach carcinogenesis probably through modulating ArR and ARNT expression in the experimental mice.

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Changes in Serum Physiological and Biochemical Parameters of Male Swiss Albino Mice After Oral Administration of Metal Oxide Nanoparticles (ZnO, CuO, and ZnO+CuO)

Biological Trace Element Research ◽

10.1007/s12011-020-02560-7 ◽

2021 ◽

Author(s):

Dicle Kargin

Keyword(s):

Metal Oxide ◽

Oral Administration ◽

Biochemical Parameters ◽

Metal Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Swiss Albino Mice ◽

Albino Mice ◽

Physiological And Biochemical

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In vivo immunomodulatory effects of the methanolic leaf extract of Gymnema sylvestre in Swiss albino mice

Archives of Biological Sciences ◽

10.2298/abs141027018a ◽

2015 ◽

Vol 67 (2) ◽

pp. 561-570 ◽

Cited By ~ 4

Author(s):

Laxmi Ahirwal ◽

Siddhartha Singh ◽

Manish Dubey ◽

Vandana Bharti ◽

Archana Mehta ◽

...

Keyword(s):

Oral Administration ◽

Leaf Extract ◽

Delayed Type Hypersensitivity ◽

Phytochemical Analysis ◽

Gymnema Sylvestre ◽

Secondary Antibody ◽

Swiss Albino Mice ◽

Flow Cytometric ◽

Albino Mice

In the present study we performed a comparative phytochemical analysis of the immunomodulating activities of the methanol leaf extract of Gymnema sylvestre (MLEGS) in Swiss albino mice. The phytochemical screening conducted on MLEGS revealed the presence of several phytoconstituents, including saponins, alkaloids, glycosides, phenols, tannins, and flavonoids. Immunomodulatory activities were determined by hemagglutination antibody (HA) titer and delayed-type hypersensitivity (DTH) tests for determining specific and non-specific immune responses. Flow cytometric techniques were performed for the estimation of B lymphocytes (CD3 and CD19) and Th2 cytokines (IL-2, IFN-? and IL-4). The response produced by oral administration of MLEGS elicited a significant reduction in a dose-related manner in the primary and secondary antibody response and DTH response. The response produced by oral administration of MLEGS elicited significant reduction in a dose-related manner in the primary and secondary antibody and DTH responses, with maximum reduction observed at 200 mg/kg-body wt. The maximal reductions in the production of CD3, CD19, IL-2, IFN-? and IL-4 were 31.59, 32.12, 29.51, 32.45 and 33.53%, respectively, at 200 mg/kg body weight. This study demonstrates that G. sylvestre exerts immunosuppressive effects on the components of the immune system of mice, and points to its significant immunomodulatory potential.

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