Intravenous N-Acetylcysteine, Hepatotoxicity and Plasma Glutathione S-Transferase in Patients with Paracetamol Overdosage

1990 ◽  
Vol 9 (3) ◽  
pp. 183-186 ◽  
Author(s):  
G.J. Beckett ◽  
J.W. Donovan ◽  
A.J. Hussey ◽  
A.T. Proudfoot ◽  
L.F. Prescott
Keyword(s):  
Liver Damage ◽  
Plasma Samples ◽  
Paracetamol Poisoning ◽  
Glutathione S Transferase ◽  
Plasma Glutathione ◽  
Paracetamol Overdosage

The concentration of glutatione S-transferase B1 (GST B1) subunits was measured in sequential plasma samples taken at frequent intervals for 48 h from ten patients with severe paracetamol poisoning who were treated with intravenous N-acetylcysteine. No significant increase in plasma GST B1 concentration was observed over the study period and within 4 h of starting treatment with N-acetylcysteine there were significant decreases in plasma GST B1 concentrations. None of the patients subsequently developed significant liver damage. At the dose used for the treatment of paracetamol poisoning, N-acetylcysteine has no hepatotoxic effects.

Plasma glutathione S-transferase measurements in patients with alcoholic cirrhosis

1987 ◽  
Vol 169 (1) ◽  
pp. 85-89 ◽  
Author(s):  
Geoffrey J. Beckett ◽  
Peter C. Hayes ◽  
Amanda J. Hussey ◽  
Ian A.D. Bouchier ◽  
John D. Hayes
Keyword(s):  
Alcoholic Cirrhosis ◽  
Glutathione S Transferase ◽  
Plasma Glutathione

Management of serious paracetamol poisoning

1988 ◽  
Vol 26 (25) ◽  
pp. 97-99
Keyword(s):  
Renal Failure ◽  
Single Dose ◽  
Alcohol Abuse ◽  
Liver Damage ◽  
Severe Pain ◽  
Paracetamol Overdose ◽  
Chronic Alcohol ◽  
Paracetamol Poisoning ◽  
Chronic Alcohol Abuse ◽  
Acute Overdose

Several hundreds of people die from paracetamol overdose each year, many perhaps unnecessarily.1 Serious poisoning usually follows acute overdose but can also occur in patients with severe pain taking large doses (over 10g/day) on consecutive days. A single dose of as little as 10g can cause hepatic or renal failure. The outcome is determined by the amount taken and, more importantly, by whether the patient comes to hospital in time for treatment to be effective.2 Chronic alcohol abuse,3 malnutrition, or taking enzyme-inducing drugs increases the risk of liver damage.4,5

scholarly journals Variability of Glutathione S-Transferase α in Human Liver and Plasma

1999 ◽  
Vol 45 (3) ◽  
pp. 355-359 ◽  
Author(s):  
Theo PJ Mulder ◽  
Daniel A Court ◽  
Wilbert HM Peters
Keyword(s):  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Human Liver ◽  
Organ Donors ◽  
Healthy Controls ◽  
Plasma Samples ◽  
Glutathione S Transferase ◽  
Glutathione S Transferases ◽  
The Mean ◽  
Exogenous Compounds

Abstract Background: Glutathione S-transferases are a family of enzymes involved in the binding, transport, and detoxification of a wide variety of endogenous and exogenous compounds. Little information is available about the variability of class α glutathione S-transferases in human liver, where they are highly expressed, or in serum. Methods: Both total class α glutathione S-transferase (GST-α, composed of GSTA1-1, GSTA1-2, and GSTA2-2) as well as GSTA1-1 concentrations were measured by specific and sensitive ELISA in liver cytosols of 35 organ donors and in plasma samples of 350 healthy controls. Results: The mean total GST-α and GSTA1-1 in liver cytosols were 25.1 ± 9.4 and 10.7 ± 5.3 μg/mg protein, respectively, and did not correlate with activities of aspartate aminotransferase or alanine aminotransferase. The mean total GST-α in liver was significantly higher in females compared with males (28.8 ± 10.0 vs 22.0 ± 7.8 μg/mg protein; P <0.05). In contrast, the median total GST-α in plasma was lower in females compared with males (2.0 and 2.8 μg/L, respectively; P <0.0001). The median ratios for GSTA1-1/total GST-α in liver and plasma were 0.42 and 0.58, respectively. Conclusions: GSTA1-1 constitutes approximately one-half of the total amount of α class GSTs in human plasma and liver. Total GST-α values are higher in female liver but lower in plasma compared with the respective values in males.

scholarly journals Liver damage and impaired glucose tolerance after paracetamol overdosage.

BMJ ◽  
1966 ◽  
Vol 2 (5512) ◽  
pp. 506-507 ◽  
Author(s):  
J. S. Thomson ◽  
L. F. Prescott
Keyword(s):  
Glucose Tolerance ◽  
Impaired Glucose Tolerance ◽  
Liver Damage ◽  
Paracetamol Overdosage

scholarly journals THE EFFECTS OF DIFFERENT STORING TEMPERATURES ON THE ACTIVITY OF SHEEP BLOOD AND PLASMA GLUTATHIONE PEROXIDASE

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Dušan BOŠNJAKOVIĆ ◽  
Aleksandra PETROVIĆ ◽  
Olivera VALČIĆ ◽  
Ivan JOVANOVIĆ ◽  
Svetlana MILANOVIĆ
Keyword(s):  
Glutathione Peroxidase ◽  
Sheep Erythrocyte ◽  
Plasma Samples ◽  
Sheep Blood ◽  
Plasma Glutathione Peroxidase ◽  
Plasma Glutathione ◽  
The Stability

The aim of the study was to determine the stability of the activity ofglutathione peroxidases 1 and 3 during storage at +40C and -180C. Blood sampleswere taken from eight sheep and the activity of the enzyme was determined in theplasma (GPx3) and erythrocytes (GPx1) on the first, third, fifth and seventh day insamples stored at +40C and after one and three months in samples stored at -180C.GPx3 activity decreased significantly during storage at both temperatures, whileGPx1 remained steady even after three months of storage at -180C. Obtained resultsindicate that GPx3 activity has to be determined in fresh sheep plasma samples,while the activity of sheep erythrocyte GPx1 can be determined even after 3 monthsof storage at -180C.

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