Sanguinarine Induces Apoptosis of HT-29 Human Colon Cancer Cells via the Regulation of Bax/Bcl-2 Ratio and Caspase-9-Dependent Pathway

2012 ◽  
Vol 31 (1) ◽  
pp. 70-77 ◽  
Author(s):  
Jun Sik Lee ◽  
Won-Kyo Jung ◽  
Myung Ho Jeong ◽  
Taek Rim Yoon ◽  
Hyung Keun Kim
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
B Cell Lymphoma ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Beneficial Effects ◽  
Anticancer Properties ◽  
Human Colon Cancer Cells ◽  
Ht 29

Sanguinarine is an alkaloid obtained from the bloodroot plant Sanguinaria canadensis and has beneficial effects on oxidative stress and inflammatory disorders. Previous reports have demonstrated that sanguinarine also exhibit anticancer properties. In the current study, we investigated the effects of sanguinarine on HT-29 human colon cancer cells. It was observed that sanguinarine treatment induces a dose-dependent increase in apoptosis of human colon cancer cells. We also investigated the effects of sanguinarine on the expression of apoptosis-associated proteins, and the results revealed that there was an increase in Bax and a decrease in B-cell lymphoma 2 (Bcl-2) protein levels. Moreover, sanguinarine treatment significantly increases the activation of caspases 3 and 9 that are the key executioners in apoptosis. Our results suggest that sanguinarine induces apoptosis of HT-29 human colon cancer cells and may have a potential therapeutic use in the treatment of human colon cancer.

scholarly journals Olive Fruit Extracts Inhibit Proliferation and Induce Apoptosis in HT-29 Human Colon Cancer Cells

2006 ◽  
Vol 136 (10) ◽  
pp. 2553-2557 ◽  
Author(s):  
M. Emília Juan ◽  
Uwe Wenzel ◽  
Valentina Ruiz-Gutierrez ◽  
Hannelore Daniel ◽  
Joana M. Planas
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Olive Fruit ◽  
Fruit Extracts ◽  
Human Colon Cancer Cells ◽  
Ht 29

scholarly journals Arachidonic acid induces ER stress and apoptosis in HT-29 human colon cancer cells

2020 ◽  
Vol 24 (5) ◽  
pp. 260-266
Author(s):  
Sijeong Bae ◽  
Min-Kyoung Kim ◽  
Hong Seok Kim ◽  
Young-Ah Moon
Keyword(s):  
Colon Cancer ◽  
Arachidonic Acid ◽  
Er Stress ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells ◽  
Ht 29

scholarly journals Synergistic Combinations of Curcumin, Sulforaphane, and Dihydrocaffeic Acid against Human Colon Cancer Cells

2020 ◽  
Vol 21 (9) ◽  
pp. 3108 ◽  
Author(s):  
Jesús Santana-Gálvez ◽  
Javier Villela-Castrejón ◽  
Sergio O. Serna-Saldívar ◽  
Luis Cisneros-Zevallos ◽  
Daniel A. Jacobo-Velázquez
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Synergistic Effects ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Acid Metabolite ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells ◽  
Synergistic Combinations ◽  
Ht 29

Nutraceutical combinations that act synergistically could be a powerful solution against colon cancer, which is the second deadliest malignancy worldwide. In this study, curcumin (C), sulforaphane (S), and dihydrocaffeic acid (D, a chlorogenic acid metabolite) were evaluated, individually and in different combinations, over the viability of HT-29 and Caco-2 colon cancer cells, and compared against healthy fetal human colon (FHC) cells. The cytotoxic concentrations to kill 50%, 75%, and 90% of the cells (CC50, CC75, and CC90) were obtained, using the MTS assay. Synergistic, additive, and antagonistic effects were determined by using the combination index (CI) method. The 1:1 combination of S and D exerted synergistic effects against HT-29 at 90% cytotoxicity level (doses 90:90 µM), whereas CD(1:4) was synergistic at all cytotoxicity levels (9:36–34:136 µM) and CD(9:2) at 90% (108:24 µM) against Caco-2 cells. SD(1:1) was significantly more cytotoxic for cancer cells than healthy cells, while CD(1:4) and CD(9:2) were similarly or more cytotoxic for healthy cells. Therefore, the SD(1:1) combination was chosen as the best. A model explaining SD(1:1) synergy is proposed. SD(1:1) can be used as a basis to develop advanced food products for the prevention/co-treatment of colon cancer.

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