scholarly journals Intrathecal Morphine Suppresses Immune Function in Rats with Inflammatory-induced Pain

2007 ◽  
Vol 35 (5) ◽  
pp. 626-636 ◽  
Author(s):  
W Zou ◽  
Q Guo ◽  
E Wang ◽  
J Cai ◽  
Z Cheng
Keyword(s):  
Immune Function ◽  
T Lymphocyte ◽  
Intrathecal Morphine ◽  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Cell Activity ◽  
Lymphocyte Function ◽  
Dependent Manner ◽  
T Lymphocyte Subsets ◽  
Dose Dependent

Acute and chronic systemic administration of morphine is known to suppress immune function; however, the effect of chronic intrathecal (IT) morphine on immune function in inflammatory-induced pain is still unclear. This study examined the effects on the immune system of IT morphine in rats with formalin-induced pain. Lumbar IT catheters were implanted in rats and saline or 2.5, 5.0 or 10.0 μg/h morphine were administered for 7 days. On the last day, formalin-induced inflammatory pain was induced in rat hind paws and pain intensity was assessed. Rat spleens were then harvested for immune function assay. The IT morphine induced a dose-dependent analgesic effect and lactic acid dehydrogenase release assay showed dose-dependent suppression of natural killer cell activity. Concanavalin-A-induced splenocyte proliferation assay showed IT morphine to suppress T lymphocyte function in a dose-dependent manner. Flow cytometry showed IT morphine significantly to decrease T lymphocyte function and the percentages of T lymphocyte subsets in a dose-dependent manner. Hence, in inflammatory-induced pain IT morphine was found to suppress immune function. Chronic IT morphine should be used cautiously to treat chronic pain in immunocompromised cases.

Effects of glutamine on the immune system: influence of muscular exercise and HIV infection

1995 ◽  
Vol 79 (1) ◽  
pp. 146-150 ◽  
Author(s):  
T. Rohde ◽  
H. Ullum ◽  
J. P. Rasmussen ◽  
J. H. Kristensen ◽  
E. Newsholme ◽  
...  
Keyword(s):  
Proliferative Response ◽  
Mononuclear Cells ◽  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Cell Activity ◽  
Control Group ◽  
Dependent Manner ◽  
Killer Cell Activity ◽  
Hiv Seropositive

Glutamine increased the proliferative response and the lymphokine-activated killer cell activity of blood mononuclear cells isolated from normal healthy subjects (n = 6) in a dose-dependent manner, with optimum at 0.3–1.0 mM. The relative fraction of CD3+, CD4+, CD8+, CD14+, CD16+, and CD19+ cells was not changed by glutamine at a concentration of 0.6 mM, except in the phytohemagglutinin-stimulated proliferation experiment where the fraction of CD4+, and therefore CD3+ cells, increased. The natural killer cell activity was not influenced by glutamine. Human immunodeficiency virus (HIV)-seropositive subjects (n = 8) who performed concentric bicycle exercise for 1 h at 75% of maximal O2 consumption had an overall lower phytohemagglutinin-stimulated proliferative response, compared with the HIV-seronegative control group (n = 7). The proliferation during exercise was lower in both the HIV-seropositive and the HIV-seronegative group. Addition of glutamine in vitro did not normalize the lower proliferation in the HIV-seropositive group or the attenuated proliferation seen during exercise in both groups.

scholarly journals IL-17 constrains natural killer cell activity by restraining IL-15–driven cell maturation via SOCS3

2019 ◽  
Vol 116 (35) ◽  
pp. 17409-17418 ◽  
Author(s):  
Xuefu Wang ◽  
Rui Sun ◽  
Xiaolei Hao ◽  
Zhe-Xiong Lian ◽  
Haiming Wei ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Nk Cells ◽  
Natural Killer ◽  
Nk Cell ◽  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Cell Activity ◽  
Cell Maturation ◽  
Dependent Manner ◽  
Deficient Mice

Increasing evidence demonstrates that IL-17A promotes tumorigenesis, metastasis, and viral infection. Natural killer (NK) cells are critical for defending against tumors and infections. However, the roles and mechanisms of IL-17A in regulating NK cell activity remain elusive. Herein, our study demonstrated that IL-17A constrained NK cell antitumor and antiviral activity by restraining NK cell maturation. It was observed that the development and metastasis of tumors were suppressed in IL-17A–deficient mice in the NK cell-dependent manner. In addition, the antiviral activity of NK cells was also improved in IL-17A–deficient mice. Mechanistically, ablation of IL-17A signaling promoted generation of terminally mature CD27−CD11b+ NK cells, whereas constitutive IL-17A signaling reduced terminally mature NK cells. Parabiosis or mixed bone marrow chimeras from Il17a−/−and wild-type (WT) mice could inhibit excessive generation of terminally mature NK cells induced by IL-17A deficiency. Furthermore, IL-17A desensitized NK cell responses to IL-15 and suppressed IL-15–induced phosphorylation of signal transducer and activator of transcription 5 (STAT5) via up-regulation of SOCS3, leading to down-regulation of Blimp-1. Therefore, IL-17A acts as the checkpoint during NK cell terminal maturation, which highlights potential interventions to defend against tumors and viral infections.

Immunotoxicological assessment of cyclosporin A by conventional pathological techniques and immune function testing in the rat

1997 ◽  
Vol 16 (2) ◽  
pp. 79-88 ◽  
Author(s):  
RWR Crevel ◽  
P. Buckley ◽  
JA Robinson ◽  
IJ Sanders
Keyword(s):  
Cyclosporin A ◽  
Immune Function ◽  
Histopathological Examination ◽  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Cell Activity ◽  
Male Rats ◽  
Lymphoid Tissues ◽  
Lymphoproliferative Response ◽  
Function Tests

1 Groups of male rats were given different doses of cyclosporin A, ranging from the maximum tolerated dose (20 mg/kg/day) downwards, 7 days a week for 28 days using a protocol derived from OECD test guide line 407. 2 At the end of the test, one set of animals underwent a detailed necropsy and histopathological examination of lymphoid tissues. Immune function was assessed using the lymphoproliferative response and natural killer cell activity of their spleen cells. Another set of animals was immunised with sheep erythrocytes on day 25 and used to evaluate the ability to produce specific anti-sheep red blood cell antibody. 3 Cyclosporin A produced detectable effects on the immune system at all doses and at doses lower than other toxic effects. Both histopathological techniques and one of the immune function tests were able to identify changes at the lowest dose, 1.25 mg/kg/day. 4 The results of this investigation suggest that conven tional histopathological techniques, if applied to a range of lymphoid organs, are sufficient to identify potential immunotoxicants without recourse to im mune function tests.

scholarly journals Activation Effects of Polysaccharides ofFlammulina velutipesMycorrhizae on the T Lymphocyte Immune Function

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Zheng-Fei Yan ◽  
Nai-Xu Liu ◽  
Xin-Xin Mao ◽  
Yu Li ◽  
Chang-Tian Li
Keyword(s):  
Immune Function ◽  
T Lymphocyte ◽  
Interleukin 2 ◽  
Potential Effect ◽  
Dependent Manner ◽  
Health Products ◽  
Immune Health ◽  
Spleen Lymphocytes ◽  
Dose Dependent

Flammulina velutipesmycorrhizae have increasingly been produced with increasing ofF. velutipesproduction. A mouse model was thus used to examine potential effect ofF. velutipesmycorrhizae on the immune function. Fifty female Wistar mice (5-weeks-old) weighed 15–20 g were randomly allocated into five groups. Polysaccharide ofF. velutipesmycorrhizae were treated with mice and mice spleen lymphocytes. The levels of CD3+, CD4+, and CD8+T lymphocyte, interleukin-2 (IL-2), and tumor necrosis factor-a (TNF-α) were determined. The results showed that the proportions of CD3+, and CD4+T lymphocyte, the ratio of CD4+/CD8+, and the levels of IL-2 and TNF-a were significantly increased in polysaccharide ofF. velutipesmycorrhizae, while the proportion of CD8+T lymphocyte was decreased in polysaccharide ofF. velutipesmycorrhizae-dose dependent manner. Our findings indicated that a long term exposure of polysaccharide ofF. velutipesmycorrhizae could activate the T lymphocyte immune function. Polysaccharide ofF. velutipesmycorrhizae was expected to develop into the immune health products.

scholarly journals Defective Granule Exocytosis in Rab27a-Deficient Lymphocytes from Ashen Mice

2001 ◽  
Vol 152 (4) ◽  
pp. 835-842 ◽  
Author(s):  
Elias K. Haddad ◽  
Xufeng Wu ◽  
John A. Hammer ◽  
Pierre A. Henkart
Keyword(s):  
T Cells ◽  
Protein Function ◽  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Cell Activity ◽  
Target Cells ◽  
Lymphocyte Function ◽  
Cytotoxic Lymphocyte ◽  
Granule Exocytosis ◽  
Rab Protein

Because mutations in Rab27a have been linked to immune defects in humans, we have examined cytotoxic lymphocyte function in ashen mice, which contain a splicing mutation in Rab27a. Ashen cytotoxic T lymphocytes (CTLs) showed a >90% reduction in lytic activity on Fas-negative target cells compared with control C3H CTLs, and ashen natural killer cell activity was likewise diminished. Although their granule-mediated cytotoxicity pathway is profoundly defective, ashen CTLs displayed a normal FasL–Fas cytotoxicity pathway. The CD4/8 phenotype of ashen T cells and their proliferative responses were similar to controls. Ashen CTLs had normal levels of perforin and granzymes A and B and normal-appearing perforin-positive granules, which polarized upon interaction of the CTLs with anti–CD3-coated beads. However, rapid anti–CD3-induced granule secretion was drastically defective in both CD8+ and CD4+ T cells from ashen mice. This defect in exocytosis was not observed in the constitutive pathway, as T cell receptor–stimulated interferon-γ secretion was normal. Based on these results and our demonstration that Rab27a colocalizes with granzyme B-positive granules and is undetectable in ashen CTLs, we conclude that Rab27a is required for a late step in granule exocytosis, compatible with current models of Rab protein function in vesicle docking and fusion.

scholarly journals Influence of galacto-oligosaccharide mixture (B-GOS) on gut microbiota, immune parameters and metabonomics in elderly persons

2015 ◽  
Vol 114 (4) ◽  
pp. 586-595 ◽  
Author(s):  
Jelena Vulevic ◽  
Aleksandra Juric ◽  
Gemma E. Walton ◽  
Sandrine P. Claus ◽  
George Tzortzis ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Immune Function ◽  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Cell Activity ◽  
Elderly Persons ◽  
Elderly Age ◽  
Double Blind ◽  
Bacterial Populations ◽  
Age Related

It is recognised that ageing induces various changes to the human colonic microbiota. Most relevant is a reduction in bifidobacteria, which is a health-positive genus. Prebiotics, such as galacto-oligosaccharides (GOS), are dietary ingredients that selectively fortify beneficial gut microbial groups. Therefore, they have the potential to reverse the age-related decline in bifidobacteria and modulate associated health parameters. We assessed the effect of GOS mixture (Bimuno (B-GOS)) on gut microbiota, markers of immune function and metabolites in forty elderly (age 65–80 years) volunteers in a randomised, double-blind, placebo (maltodextrin)-controlled, cross-over study. The intervention periods consisted of 10 weeks with daily doses of 5·5 g/d with a 4-week washout period in between. Blood and faecal samples were collected for the analyses of faecal bacterial populations and immune and metabolic biomarkers. B-GOS consumption led to significant increases in bacteroides and bifidobacteria, the latter correlating with increased lactic acid in faecal waters. Higher IL-10, IL-8, natural killer cell activity and C-reactive protein and lower IL-1β were also observed. Administration of B-GOS to elderly volunteers may be useful in positively affecting the microbiota and some markers of immune function associated with ageing.

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