Effects of Phenylephrine on Transplanted Submandibular Gland

2006 ◽  
Vol 85 (12) ◽  
pp. 1106-1111 ◽  
Author(s):  
B. Xiang ◽  
Y. Zhang ◽  
Y.M. Li ◽  
K. Zhang ◽  
Y.Y. Zhang ◽  
...  
Keyword(s):  
Submandibular Gland ◽  
Apical Membrane ◽  
Rabbit Model ◽  
Acinar Cells ◽  
Salivary Secretion ◽  
Secretory Function ◽  
Aquaporin 5 ◽  
Receptor Signal ◽  
Wharton’S Duct ◽  
Structural Injury

Autotransplantation of the submandibular gland is a potential treatment for severe kerato-conjunctivitis sicca. However, one of the major barriers to this procedure is that secretions from the transplanted gland decrease shortly after the operation, which may lead to obstruction of Wharton’s duct, or even to transplantation failure. Using a rabbit model, we investigated whether phenylephrine could improve the secretion from the transplanted gland. We found that phenylephrine treatment significantly reversed the decrease in salivary secretion after transplantation, enhanced the expressions of α1A-, α1B-, and α1D-adrenoceptor mRNA, and ameliorated atrophy of acinar cells. Furthermore, phenylephrine also induced translocation of aquaporin-5 from the cytoplasm to the apical membrane, and increased the levels of phospho-ERK1/2, ERK1/2, phospho-PKCζ, and PKCζ in the transplanted gland. These results indicate that phenylephrine treatment moderates structural injury and improves secretory function in the transplanted submandibular gland through promoting α1-adrenoceptor expression and post-receptor signal transduction.

Isoproterenol Improves Secretion of Transplanted Submandibular Glands

2009 ◽  
Vol 88 (5) ◽  
pp. 477-482 ◽  
Author(s):  
Y.M. Li ◽  
Y. Zhang ◽  
L. Shi ◽  
B. Xiang ◽  
X. Cong ◽  
...  
Keyword(s):  
Submandibular Gland ◽  
Binding Capacity ◽  
Keratoconjunctivitis Sicca ◽  
Acinar Cells ◽  
Salivary Flow ◽  
Secretory Function ◽  
Receptor Signal ◽  
Maximum Binding Capacity ◽  
Wharton’S Duct ◽  
Structural Injury

Autotransplantation of the submandibular gland is effective for severe keratoconjunctivitis sicca. However, most transplants show decreased secretion shortly after the operation, which leads to obstruction of Wharton’s duct. The hypothesis that decreased catecholamine release due to denervation contributes to hypofunction in the early phase was tested in transplanted glands in rabbits. We found that salivary flow, expression of β1- and β2-adrenoceptor, and the maximum binding capacity were markedly decreased in the transplanted glands. Isoproterenol significantly reversed the decreased secretion, enhanced the expressions of β1- and β2-adrenoceptor, and ameliorated the atrophy of acinar cells. The contents of cAMP and phospho-ERK 1/2 were increased after isoproterenol treatment. These results indicate that lack of β-adrenoceptor stimulation is involved in early dysfunction of the transplanted gland. Isoproterenol treatment moderates structural injury and improves secretory function in the transplanted submandibular gland through up-regulating β1- and β2-adrenoceptor expression and post-receptor signal transduction.

scholarly journals Asymptomatic Large Sialolith of Wharton's Duct

2015 ◽  
Vol 4 (2) ◽  
pp. 143-145
Author(s):  
Somayyeh Azimi ◽  
Jamileh Taheri Begom ◽  
Zahra Elmi Rankohi
Keyword(s):  
Salivary Gland ◽  
Antibiotic Therapy ◽  
Submandibular Gland ◽  
Salivary Secretion ◽  
Transoral Approach ◽  
Cross Sectional ◽  
Wharton’S Duct ◽  
Salivary Gland Stones

ABSTRACT Aim Giant salivary gland stones (more than 15 mm) considered to be rare and a few of well-documented studies report it. In this literature, we report an asymptomatic, 15 mm sialolith in a 47-year-old man with whole procedure of treatment. Materials and methods A patient without symptom of Wharton's duct sialolith was detected and treated. Occlusal cross-sectional of mandibule film was used to guide of surgery, transoral approach was made to dissection of stone. Results Fifteen millimeters yellow sialolith was obtained, antibiotic therapy done for 1 week was after surgery and 2-month follow-up was done. Conclusion Sialoliths are responsible for the obstruction of salivary secretion. More than 80 to 90% of sialoliths, occurs in the submandibular glandular parenchyma or its duct. There is man predilection in parotid or submandibular sialolith. Giant salivary gland stones (more than 15 mm) considered to be rare and a few of well-documented studies report it. Removal of sialolith with intraloral approach is treatment of choice in the anterior duct submandibular gland. How to cite this article Begom JT, Azimi S, Rankohi ZE. Asymptomatic Large Sialolith of Wharton's Duct. Int J Experiment Dent Sci 2015;4(2):143-145.

scholarly journals Autoradiographic localization of dihydrotestosterone binding in the major salivary glands and other androgen-responsive organs of the mouse.

1987 ◽  
Vol 35 (10) ◽  
pp. 1053-1058 ◽  
Author(s):  
J I Morrell ◽  
E W Gresik ◽  
T Barka
Keyword(s):  
Growth Factor ◽  
Salivary Glands ◽  
Submandibular Gland ◽  
Cellular Localization ◽  
Incidental Finding ◽  
Acinar Cells ◽  
Unexpected Finding ◽  
Cervical Lymph Nodes ◽  
Major Salivary Glands ◽  
Duct Cells

Mouse submandibular glands show an androgen-dependent sexual dimorphism, reflected in higher concentrations in males than in females of bioactive peptides, such as epidermal growth factor (EGF), nerve growth factor, and renin in the cells of the granular convoluted tubules (GCT). Biochemical studies have demonstrated androgen receptors in submandibular gland and other androgen-responsive organs in mouse. We have determined the cellular localization of these receptors using steroid autoradiography. Fifteen adult gonadectomized male mice were injected intravenously with 0.13 microgram or 0.26 microgram [3H]-dihydrotestosterone (SA 135 Ci/mM); some animals were pre-treated with cyclocytidine to stimulate secretion by GCT cells. Animals were killed 15 min, 1, 2, or 3 hr after isotope injection. Steroid autoradiographs were prepared, and some were stained immunocytochemically for EGF. Of the different cell types of submandibular gland, the acinar cells most frequently and intensely concentrated [3H]-DHT; GCT cells also concentrated the hormone, as did a small number of striated duct cells. In the other major salivary glands, the only cells that concentrated the androgen were interlobular striated duct cells in sublingual gland. In prostate, anterior pituitary, and brain a large number of cells concentrated androgen, as has been previously reported. Androgen binding by the GCT cells was a predictable finding, since androgen-induced alterations in composition and form of these cells are well documented. The intense androgen concentration by the acinar cells was an unexpected finding and suggests a hitherto unknown androgen regulation of these cells. An incidental finding was intense concentration of [3H]-DHT in the nuclei of the endothelial cells of the post-capillary venules of the cervical lymph nodes.

Three-Dimensional Localization of DNA Synthesis in Secretory Elements of Adult Female Mouse Submandibular Gland

1990 ◽  
Vol 4 (1) ◽  
pp. 34-44 ◽  
Author(s):  
P.C. Denny ◽  
Y. Chai ◽  
D.K. Klauser ◽  
P.A. Denny
Keyword(s):  
Dna Synthesis ◽  
Submandibular Gland ◽  
Adult Female ◽  
Female Mouse ◽  
Three Dimensional ◽  
Acinar Cells ◽  
Single Pulse ◽  
Duct System ◽  
Duct Cells ◽  
Structural Relationships

A system based in part on three-dimensional structural relationships is described for precisely characterizing the location of cells within secretory complexes of the adult female mouse submandibular gland. The pattern of DNA synthesis during a 90-minute pulse with 3H-thymidine was characterized based upon the above system. Seventy-eight percent of all radiolabeled nuclei were found in the intercalated duct system. One-half of these were in second-order intercalated ducts. DNA synthesis was also observed in acinar cells, granular intercalated duct cells, striated granular duct cells, and granular duct cells. Some secretory complexes contained multiple radiolabeled nuclei, with some of these nuclei in a side-by-side configuration. Approximately one-half of all secretory complexes contained radiolabeled nuclei. A second survey of the frequency of complexes containing radiolabeled nuclei was conducted following four pulses at eight-hour intervals over a 26-hour period. Only about 30% of all complexes contained radiolabeled nuclei. This reduction in the frequency of radiolabeled nuclei when compared with the single pulse suggests the possibility of individual variation. However, a more prolonged period of daily injections for nine days with 3H-thymidine resulted in all but one of the secretory complexes containing radiolabeled nuclei. This latter observation suggests that cell addition in adult submandibular glands is widespread.

A neurokinin-1 receptor antagonist reduced hypersalivation and gastric contractility related to emesis in dogs

1998 ◽  
Vol 275 (5) ◽  
pp. G1193-G1201 ◽  
Author(s):  
Naohiro Furukawa ◽  
Hiroyuki Fukuda ◽  
Mizue Hatano ◽  
Tomoshige Koga ◽  
Yasuteru Shiroshita
Keyword(s):  
Receptor Antagonist ◽  
Submandibular Gland ◽  
Nerve Stimulation ◽  
Vagal Stimulation ◽  
Salivary Secretion ◽  
Lingual Nerve ◽  
Afferent Pathway ◽  
Nerve Activity ◽  
Gastric Corpus ◽  
Neurokinin 1

The roles of tachykinin neurokinin-1 (NK1) receptors in the induction of fictive retching, hypersalivation, and gastric responses associated with emesis induced by abdominal vagal stimulation were studied in paralyzed, decerebrated dogs. Vagal stimulation induced gradual increases in salivary secretion and activity of the parasympathetic postganglionic fibers to the submandibular gland, relaxation of the gastric corpus and antrum, and fictive retching. However, hypersalivation and increased nerve activity were suppressed and antral contractility was enhanced during fictive retching. An NK1 receptor antagonist, GR-205171, abolished the enhancement of antral contractility and fictive retching but had no effect on corpus and antral relaxation. Hypersalivation and increased nerve activity were inhibited by GR-205171 but were not completely abolished. Reflex salivation by lingual nerve stimulation was unaffected. These results suggest that GR-205171 acts on the afferent pathway in the bulb and diminishes hypersalivation and antral contraction related to emesis as well as fictive retching but does not affect gastric relaxation or hypersalivation induced by the vagovagal, vagosalivary, and linguosalivary reflexes.

scholarly journals Switching to normal diet reverses kwashiorkor-induced salivary impairments via increased nitric oxide level and expression of aquaporin 5 in the submandibular glands of male Wistar rats

2019 ◽  
Vol 44 (4) ◽  
pp. 365-372
Author(s):  
Taye Jemilat Lasisi ◽  
Shehu Tijani Shittu ◽  
Akinola Rasak Alada
Keyword(s):  
Nitric Oxide ◽  
Total Protein ◽  
Immunoglobulin A ◽  
Salivary Secretion ◽  
Normal Diet ◽  
Secretion Rate ◽  
Control Group ◽  
Aquaporin 5 ◽  
Submandibular Glands ◽  
Nitric Oxide Level

Kwashiorkor, a form of malnutrition, has been shown to cause impaired salivary secretion. However, there is dearth of information on the mechanism that underlies this complication. Also, whether returning to normal diet after kwashiorkor will reverse these complications or not is yet to be discerned. Thus, this study aimed at assessing the mechanisms that underlie kwashiorkor-induced salivary impairments and to evaluate the effects of switching back to normal-diet on kwashiorkor-induced salivary impairments. Weaning rats were randomly divided into 3 groups (control group, kwashiorkor group (KG), re-fed kwashiorkor group (RKG)) of 7 rats each. The control group had standard rat chow while the KG and RKG were fed 2% protein diet for 6 weeks to induce kwashiorkor. The RKG had their diet changed to standard rat-chow for another 6 weeks. Blood and stimulated saliva samples were collected for the analysis of total protein, electrolytes, amylase, immunoglobulin A (IgA) secretion rate, leptin, and ghrelin. Tissue total protein, nitric oxide level, expressions of Na+/K+-ATPase, muscarinic (M3) receptor, and aquaporin 5 in the submandibular glands were also determined. Data were presented as means ± SEM and compared using ANOVA with Tukey’s post hoc test. RKG showed improved salivary function evidenced by reduced salivary lag-time and potassium and increased flow rate, sodium, amylase, IgA secretion rate, leptin, submandibular nitric oxide level, and aquaporin 5 expression compared with KG. This study for the first time demonstrated that kwashiorkor caused significant reduction in salivary secretion through reduction of nitric oxide level and aquaporin 5 expression in submandibular salivary glands. Normal-diet re-feeding after kwashiorkor returned salivary secretion to normal.

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