scholarly journals Hypoxia Induces Expression and Activation of AMPK in Rat Dental Pulp Cells

2007 ◽  
Vol 86 (9) ◽  
pp. 903-907 ◽  
Author(s):  
Y. Fukuyama ◽  
K. Ohta ◽  
R. Okoshi ◽  
M. Suehara ◽  
H. Kizaki ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Dental Pulp ◽  
Hypoxia Inducible Factor ◽  
Ampk Activation ◽  
Dental Pulp Cells ◽  
Atp Production ◽  
Pulp Cells ◽  
Subunit Isoforms ◽  
Amp Activated Protein Kinase ◽  
The Relationship

AMP-activated protein kinase (AMPK) is a stress-responsive enzyme involved in cell adaptation to an energy crisis. We hypothesized that hypoxia suppresses oxidative phosphorylation and ATP production, resulting in AMPK activation to protect cells. We investigated the effects of hypoxia on cell proliferation, the expression of AMPK and hypoxia-inducible factor 1α (HIF-1α), the activation of AMPK, and the relationship between AMPK and HIF-1α expression in rat dental pulp RPC-C2A cells. AMPK in the cells was composed of catalytic α1, and regulatory β1 and γ1 subunit isoforms. Cell proliferation was initially suppressed under hypoxia, but it increased thereafter, together with an increase in the expression of AMPK and HIF-1α, and the activation of AMPK. Down-regulation of AMPKα1 by siRNA inhibited cell proliferation under both normoxia and hypoxia, revealing that AMPK induction and activation were required for cell proliferation, although HIF-1α expression under hypoxia was not affected.

Retracted: IL ‐1 β ‐induced matrix metalloproteinase‐3 regulates cell proliferation in rat dental pulp cells

Oral Diseases ◽  
2013 ◽  
Vol 21 (1) ◽  
pp. 97-105 ◽  
Author(s):  
N Ozeki ◽  
H Yamaguchi ◽  
T Hiyama ◽  
R Kawai ◽  
K Nakata ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Matrix Metalloproteinase ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Matrix Metalloproteinase 3 ◽  
Pulp Cells

scholarly journals Effect of Magnesium on Dentinogenesis of Human Dental Pulp Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Rania M. Salem ◽  
Chang Zhang ◽  
Laisheng Chou
Keyword(s):  
Cell Proliferation ◽  
Dental Pulp ◽  
Cell Attachment ◽  
Proliferation Rate ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Pulp Capping ◽  
Alp Activity ◽  
Human Dental Pulp ◽  
Pulp Cells

Introducing therapeutic ions into pulp capping materials has been considered a new approach for enhancing regeneration of dental tissues. However, no studies have been reported on its dentinogenic effects on human dental pulp cells (HDPCs). This study was designed to investigate the effects of magnesium (Mg2+) on cell attachment efficiency, proliferation, differentiation, and mineralization of HDPCs. HDPCs were cultured with 0.5 mM, 1 mM, 2 mM, 4 mM, and 8 mM concentrations of supplemental Mg2+ and 0 mM (control). Cell attachment was measured at 4, 8, 12, 16, and 20 hours. Cell proliferation rate was evaluated at 3, 7, 10, 14, and 21 days. Crystal violet staining was used to determine cell attachment and proliferation rate. Alkaline phosphatase (ALP) activity was assessed using the fluorometric assay at 7, 10, and 14 days. Mineralization of cultures was measured by Alizarin red staining. Statistical analysis was done using multiway analysis of variance (multiway ANOVA) with Wilks’ lambda test. Higher cell attachment was shown with 0.5 mM and 1 mM at 16 hours compared to control ( P < 0.0001 ). Cells with 0.5 mM and 1 mM supplemental Mg2+ showed significantly higher proliferation rates than control at 7, 10, 14, and 21 days ( P < 0.0001 ). However, cell proliferation rates decreased significantly with 4 mM and 8 mM supplemental Mg2+ at 14 and 21 days ( P < 0.0001 ). Significantly higher levels of ALP activity and mineralization were observed in 0.5 mM, 1 mM, and 2 mM supplemental Mg2+ at 10 and 14 days ( P < 0.0001 ). However, 8 mM supplemental Mg2+ showed lower ALP activity compared to control at 14 days ( P < 0.0001 ), while 4 mM and 8 mM supplemental Mg2+showed less mineralization compared to control ( P < 0.0001 ). The study indicated that the optimal (0.5–2 mM) supplemental Mg2+ concentrations significantly upregulated HDPCs by enhancing cell attachment, proliferation rate, ALP activity, and mineralization. Magnesium-containing biomaterials could be considered for a future novel dental pulp-capping additive in regenerative endodontics.

scholarly journals Effect of Luteolin and Apigenin on the Expression of Oct-4, Sox2, and c-Myc in Dental Pulp Cells withIn VitroCulture

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Lu Liu ◽  
Zhengjun Peng ◽  
Zezhen Xu ◽  
Xi Wei
Keyword(s):  
Cell Proliferation ◽  
Cell Fate ◽  
Dental Pulp ◽  
Cell Fate Determination ◽  
Dental Pulp Cells ◽  
Dental Tissue ◽  
Fate Determination ◽  
Specific Differentiation ◽  
Pulp Cells

Introduction. Dental pulp cells (DPCs) are promising cell source for dental tissue regeneration. Recently, small molecules which optimize microenvironment or activate the reprogramming network provide a new way to enhance the pluripotency. Two promising bioflavonoids luteolin and apigenin were reported to enhance reprogramming efficiency in mouse embryonic fibroblast (MEF). However, their effect and underlying mechanism in cell fate determination of human DPCs remain unclear.Methods. To elucidate the effect of luteolin and apigenin on the cell fate determination of DPCs, we explored the cell proliferation, cell cycle, senescence, apoptosis, expression of pluripotency markers Oct-4, Sox2, and c-Myc, and multilineage differentiation capability of DPCs with luteolin or apigenin treatment.Results. We demonstrated that luteolin and apigenin inhibited cell proliferation, arrested DPCs in G2/M and S phase, and upregulated PI value and apoptosis. Moreover, luteolin and apigenin increased telomerase activity, maintained DPCs in a presenescent state, and activated the expression of Oct-4, Sox2, and c-Myc at a dose- and time-dependent pattern in DPCs even at late passages, albeit repressed lineage-specific differentiation.Conclusions. Addition of luteolin and apigenin in the culture medium might provide an effective way to maintain DPCs in an undifferentiated stage and inhibit lineage-specific differentiation.

scholarly journals The Preparation and Characterization of Chitosan-Based Hydrogels Cross-Linked by Glyoxal

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2449
Author(s):  
Beata Kaczmarek-Szczepańska ◽  
Olha Mazur ◽  
Marta Michalska-Sionkowska ◽  
Krzysztof Łukowicz ◽  
Anna Maria Osyczka
Keyword(s):  
Thermal Stability ◽  
Tannic Acid ◽  
Dental Pulp ◽  
Blood Compatibility ◽  
Dental Pulp Cells ◽  
Preliminary Assessment ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.

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