scholarly journals Characterization of Szovitsia callicarpa Volatile Constituents Obtained by Micro- and Hydrodistillation

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Betül Demirci ◽  
Nurgün Küçükboyacı ◽  
Nezaket Adıgüzel ◽  
K. Hüsnü Can Başer ◽  
Fatih Demirci
Keyword(s):  
Essential Oil ◽  
Plant Material ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Major Constituent ◽  
Spectroscopic Techniques ◽  
Volatile Constituents ◽  
C Nmr

The volatile constituents of Szovitsia callicarpa Fisch. & C. A. Mey. were obtained from the fruits by microdistillation, due to the limited plant material availability. Initial GC-MS analyses of the obtained material showed an unidentified major constituent with a molecular ion peak at M+ 280. After hydrodistillation of the plant material, the resulting essential oil was also analyzed by GC and GC-MS, simultaneously. In total, twenty-two compounds, representing 98.6% of the microdistilled sample, were identified, whereas seventeen components were detected in the hydrodistilled oil, representing 98.5% of the total. The GC-MS analyses showed that the samples contained an unidentified major constituent, which was further purified from the hydrodistilled essential oil by micro-column chromatography. The structure was elucidated as α-kessyl acetate using 1H- and 13C-NMR spectroscopic and mass spectroscopic techniques. Other major constituents in the analytes were determined as longipinene, longicyclene and kessane. In addition, both samples, as well as the major compound α-kessyl acetate, were tested at 1 mg/mL concentration against the pathogen Candida tropicalis and for its free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH•) by TLC-bioauthographic techniques. Both preliminary assays at the tested concentration showed no activity.

Antioxidant Metabolites from Limonium brasiliense (Boiss.) Kuntze

2004 ◽  
Vol 59 (7-8) ◽  
pp. 477-480 ◽  
Author(s):  
Ana P. Murray ◽  
Silvana Rodriguez ◽  
María A. Frontera ◽  
María A. Tomas ◽  
María C. Mulet
Keyword(s):  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Spectroscopic Methods ◽  
H Nmr ◽  
Antioxidant Metabolites ◽  
First Time ◽  
C Nmr

Abstract A free radical scavenging activity guided fractionation of the polar extract from roots of Limonium brasiliense (Plumbaginaceae) led to the isolation of five active compounds including: myricetin 3-O-α-rhamnopyranoside (1), (-)-epigallocatechin 3-O-gallate (2), (-)-epigallocatechin (3), (+)-gallocatechin (4) and gallic acid (5). These and other chemical constituents are reported for the first time for this species. The characterization of these compounds was achieved by spectroscopic methods (1H NMR, 13C NMR and UV).

scholarly journals Composition and biological assays of the leaf essential oil of Asparagus flagellaris (Kunth) Bak

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Oluwakayode O. Odeja ◽  
Michael Gabriel Ibok ◽  
Ejike O. Okpala
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Klebsiella Pneumonia ◽  
Leaf Essential Oil

Abstract Background Asparagus flagellaris leaves are ethnomedicinally used to treat syphilis, gonorrhea and other sexually transmitted diseases (STDs), with no reports on the volatile constituents. This study was aimed to quantitatively and qualitatively characterise the composition of essential oil, evaluates the free radical scavenging and antimicrobial capacity of the essential oil. Methods The essential oil was isolated by hydrodistillation method using all-glass Clevenger-type apparatus, while the identification and quantification of constituents were performed by gas chromatography-mass spectrometry (GC-MS) technique. The antioxidant activity on 2, 2-diphenyl-1-picrylhydrazyl (DPPH) was evaluated and the oil was also tested against 10 strains of microorganisms consisting of 6 bacteria: Escherichia coli, Salmonella typhi, Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis and 4 fungi: Candida albicans, Penicillium notatum, Aspergillus niger and Rhizopus spp. using broth dilution and surface plate methods, respectively. Results A pale yellow essential oil with a characteristic scent was obtained, with a yield of 0.80% (w/w). A total of 28 compounds accounting for 97.41% of the total oil contents were identified. The oil was predominated by Thymol and its derivatives, accounting for 57.48%. The most abundance (% area) constituents of the essential oil were 5-Thymyl tiglate (18.49%), Thymyl-2-methyl butyrate (17.34%), Thymol hydroquinone dimethyl ether (10.52%), Thymol methyl ether (9.42%) and 5-Propyl-1, 3-benzodioxole (4.59%). The essential oil showed a significant free radical scavenging activity compared to the standard antioxidant drugs used in this study, with % inhibition varying from 88.06 ± 0.0001 to 93.05 ± 0.0006. The leaf essential oil exhibited antimicrobial activity on all the tested organisms at 500–125 μg/mL, with an 18–10 mm inhibitory zone. Conclusion The leaf essential oil of A. flagellaris contains notable chemical compounds responsible for its antioxidant and antimicrobial activities.

scholarly journals Isolation and Characterization of Antimicrobial Compound from Stem Bark of Traditional Medicinal Plant Sonneratia apetala: Evaluation of their antimicrobial, Cytotoxic and Antioxidant Properties

2021 ◽  
Vol 38 (1) ◽  
pp. 1-5
Author(s):  
Md Arifur Rahman ◽  
Ahmed Abu Rus’d ◽  
Md Enamul Haque
Keyword(s):  
Brine Shrimp ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Gram Negative Bacteria ◽  
Isolation And Characterization

Sonneratia apetala (S. apetala) (Lythraceae) has been investigated for the isolation and characterization of antimicrobial compounds and evaluation of their biological activities. The chloroform extract of the stem bark and different partitionate of the chloroform extracts i.e. Petroleum ether soluble fraction (PESF), Ethyl acetate soluble fraction(EASF), Methanol soluble fraction(MSF) and aqueous soluble fractions (ASF) were subjected to different chromatographic techniques to isolate secondary metabolites. Successive chromatographic separation and purification yielded a total of two compounds identified and characterized as Taraxerone(1) and 5,8-dihydroxy- 6-methoxy-4,9-dioxo-1,3,4,9-tetrahydronaphthol[2,3-c]furan-1-yl acetate (2) by extensive proton NMR spectrum (1H-NMRspectrum) analysis. The different partitionate like PESF, EASF, MESF and ASF were subjected to screen their antimicrobial properties against some selected Gram positive and Gram negative bacteria and fungi, brine shrimp lethality and antioxidant activities. The maximum zone of inhibition of chloroform extract was found against Pseudomonas sp. (16mm). All fractions showed more activity against Gram negative bacteria then Gram positive bacteria. In the brine shrimp lethality bioassay, among all extracts, the petroleum ether and ethyl acetate soluble fraction showed significant lethality having the LC50 value of 7.72 μg/ml. The antioxidant activity was evaluated in terms of determination of free radical scavenging activity (DPPH assay). Among all the extracts of S. apetala the highest free radical scavenging activity showed by (Methanol soluble fraction) MESF with IC50 value 18.0 μg/ml. Bangladesh J Microbiol, Volume 38, Number 1, June 2021, pp 1-5

scholarly journals Chemical Composition and Antioxidant Activities of the Essential Oil from Tornabenea bischoffii (Apiaceae)

2011 ◽  
Vol 6 (8) ◽  
pp. 1934578X1100600
Author(s):  
Risoleta Ortet ◽  
Erik L. Regalado ◽  
Olivier P. Thomas ◽  
Jorge A. Pino ◽  
Miguel D. Fernández
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
In Vitro Assay ◽  
Cape Verde ◽  
Major Constituent ◽  
Vitro Assay

The chemical composition of Tornabenea bischoffii J. A. Schmidt essential oil from Cape Verde was studied by GC and GC/MS. Sixty volatile compounds were identified of which myristicin was the major constituent (33.6%). Using the 2,2-diphenyl-2-picrylhydrazyl free-radical scavenging method and the in vitro assay for prevention of lipid peroxidation by thiobarbituric reactive species, significant antioxidant activities were evidenced.

Flavonoids and triterpenes from Combretum fragrans with anti-inflammatory, antioxidant and antidiabetic potential

2018 ◽  
Vol 73 (5-6) ◽  
pp. 211-219 ◽  
Author(s):  
Amadou Dawé ◽  
Marius Mbiantcha ◽  
Fawai Yakai ◽  
Almas Jabeen ◽  
Muhammad Shaiq Ali ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Antidiabetic Activity ◽  
Moderate Activity ◽  
Spectroscopic Techniques ◽  
Asiatic Acid ◽  
Anti Inflammatory

Abstract Despite the well-documented benefits of Combretum fragrans in Cameroon, only few scientific works have been done on it. In this study we isolated eight compounds from the leaves extract of C. fragrans: velutin (1), belamcanidin (2), cirsilineol (3), cirsimaritin (4), 3β-acetoxy-20,24-epoxy-11,25-hydroxy-dammarane (5), combretin A (6), combretin B (7) and a mixture of arjunolic acid (8a) and asiatic acid (8b). Compounds 6 and 7 presented potent anti-inflammatory, antioxidant and antidiabetic activities. Compounds 1, 3, 5 and the mixture of 8a and 8b were significantly active, and compounds 2 and 4 presented moderate activity for reactive oxygen species inhibitory and free-radical scavenging. All compounds were isolated using chromatographic techniques; their structures were elucidated by spectroscopic techniques and their spectroscopic data compared with those of the literature. Anti-inflammatory activity was evaluated via the oxidative burst assay using a luminol-amplified chemiluminescence technique, antioxidant activity by free-radical scavenging activity (DPPH) and antidiabetic activity via α-glucosidase inhibition. All of the isolated compounds (1–8) were reported to exhibit significant antioxidant activity. Compounds 1, 3, and 5–8 exhibited potent chemiluminescence inhibition effect, and only compounds 6 and 7 inhibited α-glucosidase. Thus, C. fragrans can be used as an effective natural source of anti-inflammatory, antioxidant and antidiabetic compounds.

scholarly journals Chemical Composition of the Essential Oil of Ligularia hodgsonii and Free Radical Scavenging Activity of the Oil and Crude Extracts

2014 ◽  
Vol 9 (10) ◽  
pp. 1934578X1400901
Author(s):  
Zihao Zhou ◽  
Jun Tang ◽  
Xueping Song
Keyword(s):  
Free Radical ◽  
Chemical Composition ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Total Oil ◽  
Lower Capacity ◽  
Free Radical Scavenging Activities

The chemical composition of the essential oil from roots and rhizomes of Ligularia hodgsonii was analyzed by gas chromatography (GC) and GC-mass spectrometry (MS). Forty-seven components were identified, representing 77.0% of the total oil. The main constituents were l-(+)-ascorbic acid 2,6-dihexadecanoate (15.7%), selina-6-en-4-ol (8.4%) and 9,10-dimethyl-1,2,3,4,5,6,7,8-octahydroanthracene (6.6%). The free radical scavenging activities of the essential oil, aqueous extract, ethanolic extract, and crude polysaccharides of L. hodgsonii, as well as some of their major components, were investigated using DPPH and ABTS assays. The essential oil displayed a lower capacity to quench free radicals than the extracts; the ethanolic and aqueous extracts showed considerably higher antioxidant potential that deserves further study.

scholarly journals Artemisia dracunculus Essential Oil Chemical composition and antioxidant properties

2019 ◽  
Vol 70 (1) ◽  
pp. 59-62 ◽  
Author(s):  
Ariadna Petronela Fildan ◽  
Ioan Pet ◽  
Daniela Stoin ◽  
Gabriel Bujanca ◽  
Alexandra Teodora Lukinich-Gruia ◽  
...  
Keyword(s):  
Free Radical ◽  
Chemical Composition ◽  
Essential Oil ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Artemisia Dracunculus

Free radical scavenging activity, total phenolic content and the chemical composition of the essential oil isolated by steam distillation from Artemisia dracunculus L. was investigated. The isolation yield was 0.24% (v/w) based on the fresh plant material (leaves). GC-MS investigation identified 21 components, accounting 99.93% of the total amount. The major components were sabinene (42.38%), isoelemicin (12.91%), methyl eugenol (9.09%), elemicin (7.95%) and beta-ocimene (6.46%). The free radical scavenging activity of the essential oil of Artemisia dracunculus L. was evaluated in vitro by the DPPH assay (IC50 = 0.730 � 0.213 mg/mL), BHA and alpha-tocopherol were used as a positive control. The total phenolic content of the tarragon essential oil was evaluated by the Folin-Ciocalteu method (GAE = 0.451 � 0.001 mg/g sample). In view of these data, we consider that tarragon essential oil could represent a new antioxidants source as a reliable option to reduce the usage of synthetic additives.

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