scholarly journals Acanthoic Acid Inhibits Melanogenesis through Tyrosinase Down-regulation and Melanogenic Gene Expression in B16 Melanoma Cells

2013 ◽  
Vol 8 (10) ◽  
pp. 1934578X1300801
Author(s):  
Weon-Jong Yoon ◽  
Young-Min Ham ◽  
Hun Seok Yoon ◽  
Wook-Jae Lee ◽  
Nam Ho Lee ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Down Regulation ◽  
Melanin Biosynthesis ◽  
Acanthopanax Koreanum ◽  
B16 Melanoma Cells ◽  
Whitening Agent ◽  
Rate Limiting ◽  
Acanthoic Acid

The aim of this study was to investigate the in vitro inhibitory effects of acanthoic acid (ACAN), isolated from Acanthopanax koreanum, on melanogenesis and its related enzymes such as tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 in B16 melanoma cells. We found that ACAN significantly attenuates melanin synthesis and reduces the activity of intracellular tyrosinase, the rate-limiting melanogenic enzyme. Western blot analysis showed that ACAN also decreases tyrosinase, TRP-1, and TRP-2 protein expression. In addition, ACAN significantly decreased the expression of microphthalmia-associated transcription factor (MITF), a key regulator of melanogenesis. These results indicate that ACAN effectively inhibits melanin biosynthesis through down-regulation of MITF and thus could be useful as a new skin-whitening agent.

scholarly journals Elucidation of Melanogenesis-Associated Signaling Pathways Regulated by Argan Press Cake in B16 Melanoma Cells

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2697
Author(s):  
Thouria Bourhim ◽  
Myra O. Villareal ◽  
Chemseddoha Gadhi ◽  
Hiroko Isoda
Keyword(s):  
Signaling Pathways ◽  
Press Cake ◽  
Global Gene Expression ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Dependent Manner ◽  
Melanin Biosynthesis ◽  
B16 Melanoma Cells ◽  
Pigmentary Disorders ◽  
Argan Oil

The beneficial effect on health of argan oil is recognized worldwide. We have previously reported that the cake that remains after argan oil extraction (argan press-cake or APC) inhibits melanogenesis in B16 melanoma cells in a time-dependent manner without cytotoxicity. In this study, the global gene expression profile of B16 melanoma cells treated with APC extract was determined in order to gain an understanding of the possible mechanisms of action of APC. The results suggest that APC extract inhibits melanin biosynthesis by down-regulating microphthalmia-associated transcription factor (Mitf) and its downstream signaling pathway through JNK signaling activation, and the inhibition of Wnt/β-catenin and cAMP/PKA signaling pathways. APC extract also prevented the transport of melanosomes by down-regulating Rab27a expression. These results suggest that APC may be an important natural skin whitening product and pharmacological agent used for clinical treatment of pigmentary disorders.

Exercise effects on lung tumor metastases and in vitro alveolar macrophage antitumor cytotoxicity

1998 ◽  
Vol 274 (5) ◽  
pp. R1454-R1459 ◽  
Author(s):  
J. M. Davis ◽  
M. L. Kohut ◽  
D. A. Jackson ◽  
L. H. Colbert ◽  
E. P. Mayer ◽  
...  
Keyword(s):  
Alveolar Macrophage ◽  
Prolonged Exercise ◽  
Lung Tumor ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Intravenous Injections ◽  
B16 Melanoma Cells ◽  
Strong Trend ◽  
Tumor Metastases

This study examined the effects of moderate and prolonged exercise on 1) lung tumor metastases and 2) alveolar macrophage antitumor response in vitro. C57Bl/6 mice were assigned to either Ex-30 (30-min run), Ex-F (run to fatigue), Ex-F-24 h (run to fatigue 24 h before tumor injection), or Con (rested in lanes above the treadmill). Mice received intravenous injections of syngeneic B16 melanoma cells 30 min postexercise. Lungs were removed 7 or 10 days later, and tumor foci were counted. Ex-F had fewer tumors than either Ex-30 or Con, whereas Ex-F-24 h also showed a strong trend toward fewer tumors. The initial localization of tumor cells in the lungs after injection was not different among groups. For the in vitro experiment, mice were killed immediately after exercise or 8 h later. Alveolar macrophages were removed and cultured in vitro with B16 melanoma cells. The growth of the tumors cultured with macrophages from Ex-F was lower than Con after exercise and, to a lesser extent, 8 h later. In Ex-30, this effect was only found immediately after exercise. The data suggest that prolonged exercise has a protective effect on lung tumor metastases and enhances alveolar macrophage antitumor cytotoxicity.

scholarly journals Down-regulation of Glutathione and Bcl-2 Synthesis in Mouse B16 Melanoma Cells Avoids Their Survival during Interaction with the Vascular Endothelium

2003 ◽  
Vol 278 (41) ◽  
pp. 39591-39599 ◽  
Author(s):  
Angel Ortega ◽  
Paula Ferrer ◽  
Julian Carretero ◽  
Elena Obrador ◽  
Miguel Asensi ◽  
...  
Keyword(s):  
Vascular Endothelium ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Down Regulation ◽  
B16 Melanoma Cells

Cytotoxic effect of RA233 on hypoxic B16 melanoma cells in vitro

1988 ◽  
Vol 42 (1-2) ◽  
pp. 127-131 ◽  
Author(s):  
C.W. Stackpole ◽  
R.A. Omar ◽  
D.M. Fornabaio ◽  
Y.S. Kim
Keyword(s):  
Cytotoxic Effect ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
B16 Melanoma Cells

scholarly journals The Inhibitory Effects of Representative Chalcones Contained in Angelica keiskei on Melanin Biosynthesis in B16 Melanoma Cells

2012 ◽  
Vol 7 (8) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Enos Tangke Arung ◽  
Shoko Furuta ◽  
Kazuhiro Sugamoto ◽  
Kuniyoshi Shimizu ◽  
Hiroya Ishikawa ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Inhibitory Effects ◽  
Melanin Biosynthesis ◽  
Melanin Formation ◽  
B16 Melanoma Cells ◽  
Angelica Keiskei ◽  
Low Cytotoxicity ◽  
Antioxidant Effects

In our effort to find new whitening agents, we evaluated the effects of representative chalcones [4-hydroxyderricin (1), xanthoangelol (2), xanthoangelol H (3), deoxyxanthoangelol H (4), and deoxydihydroxanthoangelol H (5)] contained in the stem of Angelica keiskei on tyrosinase and melanin formation in B16 melanoma cells. In addition, the antioxidant effects of these chalcones in ORAC and DPPH assays were also determined. Interestingly, all chalcones (1–5) inhibit melanin formation in B16 melanoma cells, with low cytotoxicity.

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