Biochemical Effects of Aqueous Extract of Persea americana (Mill) on the Myocardium of Left Ventricle of High Salt–Fed Adult Wistar Rats

Background. The cardioprotective effects of Persea americana extract was investigated on biochemical activities of high salt–fed adult Wistar rats in this study. Method. Forty healthy Wistar rats of both sexes weighing 120 to 150 g were randomly assigned into 8 groups of 5 rats each (groups A, B, C, D, E, F, G, and H). Rats in groups A, F, G, and H were fed with standard laboratory pellets, while groups B, C, D, and E were fed on the high-salt diet for 4 weeks. Concomitantly, daily administration of 50, 100, and 150 mg/kg of the P americana extract were given orally to groups C and F, D and G, and E and H, respectively, while rats in groups A and B were administered distilled water. Blood samples were taken by cardiac puncture; concentration of sodium ion, potassium ion, nitric oxide, and activity of lactate dehydrogenase were determined. One-way analysis of variance was used to analyze data, followed by Student-Newman-Keuls (SNK) test for multiple comparison. Results. Results revealed that concentration of potassium ion and nitric oxide was significantly lower ( P < .05) in high salt–fed groups. Sodium ion concentration and activity of lactate dehydrogenase were higher in high salt–fed group while P americana prevented biochemical perturbations in other experimental groups. Conclusion. In conclusion, high salt–diet induced biochemical alterations which were significantly protected by oral administration of P americana extract.

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Effects of Aqueous Extract of Persea americana (Mill.) Leaf on the Myocardium of Left Ventricle of High Salt Fed Adult Wistar Rat

Journal of Morphological Sciences ◽

10.1055/s-0038-1660490 ◽

2018 ◽

Vol 35 (01) ◽

pp. 31-36

Author(s):

I.O. Ayoola ◽

O.A. Komolafe ◽

O.S. Saka ◽

R.A. Bejide ◽

S.O.A. Odukoya

Keyword(s):

Left Ventricle ◽

Wistar Rats ◽

Wistar Rat ◽

Persea Americana ◽

High Salt ◽

High Salt Diet ◽

Salt Diet ◽

Morphological Alterations ◽

Ketamine Anesthesia ◽

Group B

Introduction This study was designed to show that Persea americana extract possess the ability to protect the myocardium of left ventricle against injury caused by high salt diet in adult Wistar rats. Method Forty healthy Wistar rats of both sexes weighing 120–150 g were randomly assigned into 8 groups of 5 rats each (Groups A, B, C, D, E, F, G and H). Rats in groups A, F, G and H were fed with standard laboratory pellets, while groups B, C, D and E were fed on the high-salt diet for four weeks. Concomitantly, daily administration of 50 mg kg-1, 100 mg kg-1 and 150 mg kg-1 of the Persea americana extract were given orally to groups C&F, D&G and E&H respectively while rats in groups A and B were administered distilled water. The rats were sacrificed under ketamine anesthesia (30mg/kg i.m). The left ventricle of the heart was excised, processed in paraffin wax and stained with haematoxylin and eosin and Verhoeff-Van Gieson stains. One-way ANOVA was used to analyze data, followed by Student Newman-keuls (SNK) test for multiple comparison. Result Results revealed that there was statistically significant (p < 0.05) difference in body weight change across all experimental groups; which was significantly lower in high salt fed groups. It was revealed that there were morphological alterations in the myocardium of left ventricle in group B while Persea americana protected myocardium in other experimental groups. Conclusion In conclusion, high salt diet induced myocardium alterations which were significantly protected by oral administration of Persea americana extract.

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The role of poly-herbal extract in sodium chloride-induced oxidative stress and hyperlipidemia in male Wistar rats

10.21203/rs.3.rs-420441/v1 ◽

2021 ◽

Author(s):

Olumide Fadahunsi ◽

Peter Adegbola ◽

Olayemi Adebola Akintola ◽

Bamidele Stephen Ajilore ◽

olubukola sinbad Olorunnisola

Keyword(s):

Nitric Oxide ◽

Serum Lipid ◽

Antioxidant Status ◽

Wistar Rats ◽

Density Lipoprotein ◽

High Salt ◽

Herbal Extract ◽

High Salt Diet ◽

Salt Diet ◽

Male Wistar Rats

Abstract Consistent consumption of high salt diet (HSD) has been associated with increased cellular generation of free radicals which has been implicated in the derangement of some vital organs and etiology of cardiovascular disorders. This study was designed to investigate the combined effect of some commonly employed medicinal plants on serum lipid profile and antioxidant status of aorta, kidney, and liver of high salt diet-fed animals. Thirty-five male Wistar rats were divided into 5 groups of 7 animals each. Group 1 and 2 animals were fed normal rat and 16 % high salt diet only respectively. Animals in groups 3, 4, and 5 were fed 16% high salt diet with 800, 400, and 200 mg/kg bw poly-herbal extract (PHE) respectively once for 28 consecutive days. Serum low-density lipoprotein (LDL), triacylglycerol (TG), total cholesterol (TC) and high-density lipoprotein (HDL), malondialdehyde, nitric oxide, catalase, superoxide dismutase, glutathione peroxidase, glutathione concentration, and activities were assessed in the aorta, kidney, and liver. PHE (p < 0.05) significantly reduced malondialdehyde and nitric oxide concentration and increased antioxidant enzymes and glutathione activity. Elevated serum TG, TC, LDL, and TC content in HSD-fed animals were significantly (p < 0.05) reduced to normal in PHE-treated rats while HDL was significantly elevated (p < 0.05) in a concentration-dependent manner in PHE treated animals. Feeding with PHE attenuated high salt diet imposed derangement in serum lipid profile and antioxidant status in the organs of the experimental rats.

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The Role of Poly-Herbal Extract in Sodium Chloride-Induced Oxidative Stress and Hyperlipidemia in Male Wistar Rats

Medicines ◽

10.3390/medicines8060025 ◽

2021 ◽

Vol 8 (6) ◽

pp. 25

Author(s):

Olubukola Sinbad Olorunnisola ◽

Peter Ifeoluwa Adegbola ◽

Bamidele Stephen Ajilore ◽

Olayemi Adebola Akintola ◽

Olumide Samuel Fadahunsi

Keyword(s):

Nitric Oxide ◽

Serum Lipid ◽

Antioxidant Status ◽

Wistar Rats ◽

Density Lipoprotein ◽

High Salt ◽

Herbal Extract ◽

High Salt Diet ◽

Salt Diet ◽

Male Wistar Rats

Consistent consumption of high salt diet (HSD) has been associated with increased cellular generation of free radicals, which has been implicated in the derangement of some vital organs and etiology of cardiovascular disorders. This study was designed to investigate the combined effect of some commonly employed medicinal plants on serum lipid profile and antioxidant status of aorta, kidney, and liver of high salt diet-fed animals. Out of the total fifty male Wistar rats obtained, fifteen were used for acute toxicity study, while the remaining thirty-five were divided into 5 groups of 7 animals each. Group 1 and 2 animals were fed normal rat chow (NRC) and 16% high salt diet (HSD) only, respectively. Animals in groups 3, 4 and 5 were fed 16% HSD with 800, 400, and 200 mg/kg bw poly-herbal extract (PHE), respectively, once for 28 consecutive days. Serum low-density lipoprotein (LDL), triacylglycerol (TG), total cholesterol (TC) and high-density lipoprotein (HDL), malondialdehyde, nitric oxide, catalase, superoxide dismutase, glutathione peroxidase, glutathione concentration, and activities were assessed in the aorta, kidney, and liver. Poly-herbal extract (p < 0.05) significantly reduced malondialdehyde and nitric oxide concentrations and also increased antioxidant enzymes and glutathione activity. Elevated serum TG, TC, LDL, and TC content in HSD-fed animals were significantly (p < 0.05) reduced to normal in PHE-treated rats while HDL was significantly elevated (p < 0.05) in a concentration-dependent manner in PHE treated animals. Feeding with PHE attenuated high-salt diet imposed derangement in serum lipid profile and antioxidant status in the organs of the experimental rats.

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