Ultrastructural cytochemistry and radioautography of complex carbohydrates in heterophil granulocytes from rabbit bone marrow.

The subcellular route of incorporation of complex carbohydrates into rabbit heterophil primary granules and their subsequent intragranular distribution during granule maturation were studied with ultrastructural, cytochemical, and radioautographic methods. High iron diamine (HID) staining of sulfated glycoconjugates in primary granules was partially diminished after treatment with chondroitinase ABC or after removal of N-sulfate groups with nitrous acid, but was not altered by exposure to hyaluronidase, trypsin, or HCl. Subsequent thiocarbohydrazide-silver proteinate (TCH-SP) straining of thin sections increased the density of the HID reaction product. Golgi-derived spherules and very immature morular granules stained weakly with HID-TCH-SP and labeled intensely after a 10 min incubation with 35SO4. After a 60 min 35SO4 pulse and a 60 min chase, an increase in radiolabeling was observed in granules with HID stained, fused morular material, and some labeling was present in more mature rim stained granules. Fully mature granules lacked HID or HID-TCH-SP staining, but contained most of the 35SO4 labels after a 60 min pulse and 18 hr chase in vitro. Periodate-thiocarbohydrazide-silver proteinate (PA-TCH-SP) staining of unosmicated thin sections localized vicinal glycol-containing complex carbohydrates in Golgi-associated small vesicles. These vesicles lacked HID-TCH-SP staining and apparently contained neutral glycoprotein. They frequently bordered, in a rosette arrangement, the immature morular granules, but not the more mature primary granules. The PA-TCH-SP method localized complex carbohydrates in the rim of granules precursors and enclosed a spherule or morula, but failed to stain the sulfate-containing material in the morulas or spherules. PA-TCH-SP reactivity was diffusely distributed in moderately mature granules and was decreased in fully mature granules. These results indicate that heterophil primary granule contain several complex carbohydrates including O-sulfated and N-sulfated glycosaminoglycans, as well as vicinal glycol-containing glycoproteins. These complex carbohydrates are transported to immature primary granules by different Golgi-derived organelles. The complex carbohydrates are subsequently distributed differently within primary granules and become masked to staining as the granule matures.

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THE IN VITRO INCORPORATION OF THE METHYLENE CARBON ATOM OF GLYCINE INTO RABBIT BONE MARROW FATS

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)57043-5 ◽

1949 ◽

Vol 177 (2) ◽

pp. 985-986

Author(s):

Kurt I. Altman

Keyword(s):

Bone Marrow ◽

Carbon Atom ◽

Methylene Carbon ◽

Methylene Carbon Atom ◽

Rabbit Bone

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Construction of Tissue Engineering Bone with Osteoactivin Gene Transfected Rabbit Bone Marrow Stromal Cells and Porous Silk Fibroin Scaffold in Vitro

Advances in Clinical Medicine ◽

10.12677/acm.2014.42006 ◽

2014 ◽

Vol 04 (02) ◽

pp. 27-33

Author(s):

涵王

Keyword(s):

Tissue Engineering ◽

Bone Marrow ◽

Silk Fibroin ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Marrow Stromal Cells ◽

Silk Fibroin Scaffold ◽

Rabbit Bone

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Determine exogenous humanDDAH2gene function in rabbit bone marrow-derived endothelial progenitor cells in vitro

Cell Biochemistry and Function ◽

10.1002/cbf.3249 ◽

2017 ◽

Vol 35 (2) ◽

pp. 69-76

Author(s):

Sara Shoeibi ◽

Shabnam Mohammadi ◽

Hamid Reza Sadeghnia ◽

Elahe Mahdipour ◽

Majid Ghayour-Mobarhan

Keyword(s):

Bone Marrow ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Endothelial Progenitor ◽

Rabbit Bone

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In Vitro Production of Cartilage Tissue from Rabbit Bone Marrow-Derived Mesenchymal Stem Cells and Polycaprolactone Scaffold

Advances in Experimental Medicine and Biology - Tissue Engineering and Regenerative Medicine ◽

10.1007/5584_2017_133 ◽

2017 ◽

pp. 45-60 ◽

Cited By ~ 1

Author(s):

Thuy Thi-Thanh Dao ◽

Ngoc Bich Vu ◽

Liem Hieu Pham ◽

Long Van Gia ◽

Ha Thi-Ngan Le ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Cartilage Tissue ◽

In Vitro Production ◽

Rabbit Bone ◽

Vitro Production

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Accelerated myeloblast destruction and abnormal lysosome disruption in cultured bone marrow: association with indolent acute myelogenous leukemia

Blood ◽

10.1182/blood.v48.1.23.23 ◽

1976 ◽

Vol 48 (1) ◽

pp. 23-32 ◽

Cited By ~ 3

Author(s):

RF Branda ◽

HS Jacob ◽

SD Douglas ◽

CF Moldow ◽

RR Puumala

Keyword(s):

Bone Marrow ◽

Acute Myelogenous Leukemia ◽

Myelogenous Leukemia ◽

Leukemic Cells ◽

Electron Microscopic ◽

Granule Formation ◽

Microscopic Studies ◽

Acute Myelogenous ◽

Primary Granules

Abstract Despite no chemotherapy and a marrow morphologically typical of frank relapse, an acute myelogenous leukemia (AML) patient survived for nearly 1 yr. During this time she remained asymptomatic and maintained nearly normal levels of platelets and hemoglobin. Cytochemical and electron microscopic studies of her bone marrow in liquid culture revealed on several occasions a unique maturational sequence in that leukemic cells differentiated to form morphologically abnormal primary granules which appeared to rupture and cause cytolysis of these cells. In these cultures, blasts rapidly disappeared and were replaced by more mature granulocytes, in contrast to observations in cultures derived from five other patients with AML in relapse which showed persistently elevated blast counts with no evidence of maturation in vitro. These findings support the concept that in AML cell maturation is regularly impaired and in some cases also aberrant. In addition, the abnormal granule formation with autolysis of the leukemic cells observed in one patient may explain both the early cell death in vitro and this patient's relatively indolent clinical course. Similar in vitro studies may help predict atypical clinical courses in patients with AML and facilitate design of appropriate chemotherapy.

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p15s (15-kD Antimicrobial Proteins) Are Stored in the Secondary Granules of Rabbit Granulocytes: Implications for Antibacterial Synergy With the Bactericidal/Permeability-Increasing Protein in Inflammatory Fluids

Blood ◽

10.1182/blood.v89.2.672 ◽

1997 ◽

Vol 89 (2) ◽

pp. 672-679 ◽

Cited By ~ 33

Author(s):

Kol Zarember ◽

Peter Elsbach ◽

Kwang Shin-Kim ◽

Jerrold Weiss

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Extracellular Fluid ◽

Molar Ratio ◽

Immunogold Electron Microscopy ◽

Antimicrobial Proteins ◽

High Molar Ratio ◽

Rabbit Bone ◽

Polymerase Chain ◽

Primary Granules

Abstract The bactericidal potency toward complement-resistant Escherichia coli of bactericidal/permeability-increasing protein (BPI) released from polymorphonuclear leukocytes (PMNs) in glycogen-induced inflammatory peritoneal exudates of rabbits is dependent on synergy with extracellular p15s. This synergy depends on the high molar ratio of p15s to BPI in the extracellular fluid (∼50:1), which greatly exceeds the intracellular ratio (∼5:1). To explore the possible basis of the greater accumulation of p15s in inflammatory fluid, we examined the subcellular localization of BPI and p15 in PMNs. Immunogold electron microscopy confirmed the storage of BPI in primary granules and showed that p15s are stored in secondary granules. Reverse-transcription polymerase chain reaction of density-fractionated rabbit bone marrow cells verified that p15s are expressed later than BPI during myeloid differentiation. As the inflammatory response evolves, p15 mRNA appears earlier in blood and exudate cells than mRNA for BPI, consistent with release of progressively less mature precursors from bone marrow. Finally, Ca2+-ionophore–mediated exocytosis of p15s occurs more readily than release of BPI. We therefore propose that localization of a synergistic partner of BPI (p15s) in more readily released secondary granules allows the neutrophil to mobilize potent BPI-dependent antibacterial activity extracellularly without significant depletion of intracellular BPI stores.

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Influence of albuterol on erythropoietin production and erythroid progenitor cell activation

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1979.236.3.h422 ◽

1979 ◽

Vol 236 (3) ◽

pp. H422-H426 ◽

Cited By ~ 1

Author(s):

F. Przala ◽

D. M. Gross ◽

B. Beckman ◽

J. W. Fisher

Keyword(s):

Bone Marrow ◽

Progenitor Cell ◽

Cell Activation ◽

Plasma Clot ◽

Erythroid Progenitor ◽

Erythroid Progenitor Cell ◽

Adrenergic Blocker ◽

Rabbit Bone

The effect of albuterol, a potent beta2-adrenergic agonist, on kidney production of erythropoietin (Ep) was studied. Its effects on erythroid colony (CFU-E) formation in vitro in rabbit bone marrow cultures were also assessed. Albuterol produced a significant increase in plasma Ep levels in conscious rabbits following 7 h intravenous infusion (50 (microgram/kg)/min). This effect was blocked by pretreatment of the rabbits with butoxamine (5 mg/kg ip), a potent beta2-adrenergic blocker. Albuterol in doses of 10(-10) to 10(-8) M in combination with Ep was also found to produce a significant increase in the numbers of CFU-E in the plasma clot culture system of rabbit bone marrow. This effect was blocked completely by DL-propranolol (10(-8) M) and by butoxamine (10(-8) M). The data presented suggest that albuterol, a potent activator of beta2-adrenergic receptors, increases kidney production of Ep in vivo and also produces a direct effect in combination with Ep on the proliferation of the erythroid progenitor cell compartment.

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