1,25-Dihydroxyvitamin D3-induced calcium-binding protein: localization in organ-cultured embryonic chick duodenum.
1985 ◽
Vol 33
(5)
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pp. 477-479
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Keyword(s):
De Novo
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1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) induces de novo biosynthesis of a specific calcium-binding protein (CaBP) in embryonic chick duodenum in organ culture. Using a highly sensitive and specific, peroxidase-antiperoxidase immunocytochemical procedure, 1,25(OH)2D3-induced CaBP in the organ-cultured duodenum was found only in the cytoplasm of absorptive cells, corresponding to its localization in rachitic chick duodenal cells after a single injection of 1,25(OH)2D3 in vivo. This observation, along with evidence correlating CaBP with calcium transport, strongly supports the use of the embryonic chick duodenal organ culture system as a physiologically relevant model of the vitamin D-dependent calcium absorptive mechanism.
1986 ◽
Vol 261
(34)
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pp. 16106-16114
1979 ◽
Vol 196
(2)
◽
pp. 624-630
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1976 ◽
Vol 174
(2)
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pp. 738-743
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Keyword(s):
1980 ◽
Vol 202
(1)
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pp. 235-241
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1983 ◽
Vol 258
(2)
◽
pp. 1305-1310
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1988 ◽
Vol 263
(26)
◽
pp. 13112-13116
1988 ◽
Vol 260
(2)
◽
pp. 645-652
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1983 ◽
Vol 19
(5)
◽
pp. 1577-1582
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Keyword(s):
1983 ◽
Vol 31
(3)
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pp. 426-432
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