scholarly journals The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions.

1987 ◽  
Vol 35 (9) ◽  
pp. 959-969 ◽  
Author(s):  
E Kellenberger ◽  
M Dürrenberger ◽  
W Villiger ◽  
E Carlemalm ◽  
M Wurtz
Keyword(s):  
Experimental Data ◽  
Biological Material ◽  
Biological Membranes ◽  
Simple Theory ◽  
Complex Situation ◽  
Thin Sections ◽  
Thin Sectioning ◽  
Theoretical Predictions

The surface of thin sections of aldehyde-fixed biological material shows a specimen-related relief of 2-6 nm with Lowicryl. Epon sections are about three times smoother. The relief is the consequence of thin-sectioning being in reality a cleavage. Epitopes are supposed to be laid open (or set free) because cleavage follows the interfaces between protein and Lowicryl. We have developed a simple theory on this basis and have theoretically estimated the efficiency of on-section labeling and compared it with experimental data. For randomly dispersed proteins in cytoplasm, Lowicryl sections will yield significant label only when the concentration of the antigen is about 10 microM or more. The complex situation of more compact proteins, as represented by fibers, sheets, and biological membranes is discussed and the difficulty of significant calculations is explained. Pre-embedding labeling and melted cryosections should give 10-30 times more label. The possible reasons for the observed much smaller gain of not more than two to three times are discussed.

Ferritin Labeled Antibody Staining of Thin Sections

1969 ◽  
Vol 27 ◽  
pp. 420-421
Author(s):  
J. D. McLean ◽  
S. J. Singer
Keyword(s):  
Biological Material ◽  
Water Soluble ◽  
Thin Sections ◽  
Antibody Staining ◽  
Thin Sectioning ◽  
Ultrathin Sections

The successful application of ferritin labeled antibodies (F-A) to ultrathin sections of biological material has been hampered by two main difficulties. Firstly the normally used procedures for the preparation of material for thin sectioning often result in a loss of antigenicity. Secondly the polymers employed for embedding may non-specifically absorb the F-A. Our earlier use of cross-linked polyampholytes as embedding media partially overcame these problems. However the water-soluble monomers used for this method still extract many lipids from the material.

Lead aspartate: a new en bloc stain for electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 34-35
Author(s):  
J.R. Walton
Keyword(s):  
Electron Microscopy ◽  
Calcium Ion ◽  
Enzyme Reaction ◽  
Lead Citrate ◽  
Reaction Products ◽  
En Bloc ◽  
Thin Sections ◽  
Lead Salts ◽  
Thin Sectioning ◽  
High Alkalinity

In electron microscopy, lead is the metal most widely used for enhancing specimen contrast. Lead citrate requires a pH of 12 to stain thin sections of epoxy-embedded material rapidly and intensively. However, this high alkalinity tends to leach out enzyme reaction products, making lead citrate unsuitable for many cytochemical studies. Substitution of the chelator aspartate for citrate allows staining to be carried out at pH 6 or 7 without apparent effect on cytochemical products. Moreover, due to the low, controlled level of free lead ions, contamination-free staining can be carried out en bloc, prior to dehydration and embedding. En bloc use of lead aspartate permits the grid-staining step to be bypassed, allowing samples to be examined immediately after thin-sectioning.Procedures. To prevent precipitation of lead salts, double- or glass-distilled H20 used in the stain and rinses should be boiled to drive off carbon dioxide and glassware should be carefully rinsed to remove any persisting traces of calcium ion.

Albumins as Embedding Media for Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 422-423 ◽  
Author(s):  
J. L. Farrant ◽  
J. D. McLean
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
High Temperature ◽  
Biological Material ◽  
Globular Proteins ◽  
The Past ◽  
Antibody Staining ◽  
Thin Sectioning ◽  
Embedding Medium

For electron microscope techniques such as ferritin-labeled antibody staining it would be advantageous to have available a simple means of thin sectioning biological material without subjecting it to lipid solvents, impregnation with plastic monomers and their subsequent polymerization. With this aim in view we have re-examined the use of protein as an embedding medium. Gelatin which has been used in the past is not very satisfactory both because of its fibrous nature and the high temperature necessary to keep its solutions fluid. We have found that globular proteins such as the serum and egg albumins can be cross-linked so as to yield blocks which are suitable for ultrathin sectioning.

scholarly journals An Additive Model to Predict the Rheological and Mechanical Properties of Polypropylene Blends Made by Virgin and Reprocessed Components

Recycling ◽  
2021 ◽  
Vol 6 (1) ◽  
pp. 2
Author(s):  
Francesco Paolo La Mantia ◽  
Maria Chiara Mistretta ◽  
Vincenzo Titone
Keyword(s):  
Experimental Data ◽  
Mechanical Properties ◽  
Additive Model ◽  
Industrial Process ◽  
Theoretical Predictions ◽  
Experimental Values ◽  
Polypropylene Blends ◽  
Polypropylene Sample

In this work, an additive model for the prediction of the rheological and mechanical properties of monopolymer blends made by virgin and reprocessed components is proposed. A polypropylene sample has been reprocessed more times in an extruder and monopolymer blends have been prepared by simulating an industrial process. The scraps are exposed to regrinding and are melt reprocessed before mixing with the virgin polymer. The reprocessed polymer is, then, subjected to some thermomechanical degradation. Rheological and mechanical experimental data have been compared with the theoretical predictions. The results obtained showed that the values of this simple additive model are a very good fit for the experimental values of both rheological and mechanical properties.

A Technique for the Laboratory Determination of Recirculation in Single Needle Dialysis

1993 ◽  
Vol 16 (2) ◽  
pp. 63-70 ◽  
Author(s):  
N.A. Hoenich ◽  
P.T. Smirthwaite ◽  
C. Woffindin ◽  
P. Lancaster ◽  
T.H. Frost ◽  
...  
Keyword(s):  
Experimental Data ◽  
Flow Rate ◽  
Experimental Results ◽  
New Technique ◽  
Treatment Efficiency ◽  
Single Lumen ◽  
Theoretical Predictions ◽  
Lumen Catheter ◽  
A New Technique

Recirculation is an important factor in single needle dialysis and, if high, can compromise treatment efficiency. To provide information regarding recirculation characteristics of access devices used in single needle dialysis, we have developed a new technique to characterise recirculation and have used this to measure the recirculation of a Terumo 15G fistula needle and a VasCath SC2300 single lumen catheter. The experimentally obtained results agreed well with those established clinically (8.5 ± 2.4% and 18.4 ± 3.4%). The experimental results have also demonstrated a dependence on access type, pump speeds and fistula flow rate. A comparison of experimental data with theoretical predictions showed that the latter exceeded those measured with the largest contribution being due to the experimental fistula.

Wilberforce pendulum: modelling linearly damped coupled oscillations of a spring-mass system

2021 ◽  
Author(s):  
Robert Frederik Diaz Uy ◽  
Chenghao Yuan ◽  
Zhengshan Chai ◽  
Justin Khor
Keyword(s):  
Experimental Data ◽  
Angular Displacement ◽  
Beat Frequency ◽  
Vertical Displacement ◽  
Quantitative Model ◽  
Moment Of Inertia ◽  
Lagrangian Formalism ◽  
Mass System ◽  
Coupled Oscillations ◽  
Theoretical Predictions

Abstract The Wilberforce pendulum is a coupled spring-mass system, where a mass with adjustable moment of inertia is suspended from a helical spring. Energy is converted between the translational and torsional modes, and this energy conversion is most clearly observed at resonance, which occurs when the damped natural frequencies of the two oscillation modes are equal. A theoretical model—with energy losses due to viscous damping accounted for—was formulated using the Lagrangian formalism to predict the pendulum mass’ trajectory. Theoretical predictions were compared with experimental data, showing good agreement. Fourier analysis of both theoretical predictions and experimental data further corroborate the validity of our quantitative model. The dependence of oscillation features like beat frequency and maximum conversion amplitude on relevant parameters such as the initial vertical displacement, initial angular displacement and moment of inertia was also investigated and experimentally verified.

scholarly journals Bioinspired aerofoil adaptations: the next steps for theoretical models

2019 ◽  
Vol 377 (2159) ◽  
pp. 20190070 ◽  
Author(s):  
Lorna J. Ayton
Keyword(s):  
Experimental Data ◽  
Theoretical Models ◽  
Theoretical Modelling ◽  
Experimental Measurements ◽  
Far Field ◽  
Acoustic Analogy ◽  
Trailing Edge ◽  
Trailing Edge Noise ◽  
Theoretical Predictions ◽  
Field Noise

The extended introduction in this paper reviews the theoretical modelling of leading- and trailing-edge noise, various bioinspired aerofoil adaptations to both the leading and trailing edges of blades, and how these adaptations aid in the reduction of aerofoil–turbulence interaction noise. Attention is given to the agreement between current theoretical predictions and experimental measurements, in particular, for turbulent interactions at the trailing edge of an aerofoil. Where there is a poor agreement between theoretical models and experimental data the features neglected from the theoretical models are discussed. Notably, it is known that theoretical predictions for porous trailing-edge adaptations do not agree well with experimental measurements. Previous works propose the reason for this: theoretical models do not account for surface roughness due to the porous material and thus omit a key noise source. The remainder of this paper, therefore, presents an analytical model, based upon the acoustic analogy, to predict the far-field noise due to a rough surface at the trailing edge of an aerofoil. Unlike previous roughness noise models which focus on roughness over an infinite wall, the model presented here includes diffraction by a sharp edge. The new results are seen to be in better agreement with experimental data than previous models which neglect diffraction by an edge. This new model could then be used to improve theoretical predictions for far-field noise generated by turbulent interactions with a (rough) porous trailing edge. This article is part of the theme issue ‘Frontiers of aeroacoustics research: theory, computation and experiment’.

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