Autologous Chondrocytes Grown In Vitro on a Collagen Scaffold for Subsequent Articular Cartilage Repair

2005 ◽  
Vol 28 (2) ◽  
pp. 57-61
Author(s):  
Paola Narducci ◽  
Giovanna Baldini ◽  
Vittorio Grill ◽  
Vanessa Nicolin ◽  
Renato Bareggi ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Collagen Scaffold ◽  
Articular Cartilage Repair

scholarly journals A novel exogenous concentration-gradient collagen scaffold augments full-thickness articular cartilage repair

2008 ◽  
Vol 16 (9) ◽  
pp. 1083-1091 ◽  
Author(s):  
T. Mimura ◽  
S. Imai ◽  
M. Kubo ◽  
E. Isoya ◽  
K. Ando ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Concentration Gradient ◽  
Collagen Scaffold ◽  
Full Thickness ◽  
Articular Cartilage Repair

scholarly journals Reprogrammed Synovial Fluid-Derived Mesenchymal Stem/Stromal Cells Acquire Enhanced Therapeutic Potential for Articular Cartilage Repair

Cartilage ◽  
2021 ◽  
pp. 194760352110408
Author(s):  
Brian E. Walczak ◽  
Hongli Jiao ◽  
Ming-Song Lee ◽  
Wan-Ju Li
Keyword(s):  
Animal Model ◽  
Articular Cartilage ◽  
Synovial Fluid ◽  
Cartilage Repair ◽  
Stromal Cells ◽  
Cartilage Damage ◽  
Cellular Reprogramming ◽  
Human Mscs ◽  
Articular Cartilage Repair

Objectives Functions of mesenchymal stem/stromal cells (MSCs) are affected by patient-dependent factors such as age and health condition. To tackle this problem, we used the cellular reprogramming technique to epigenetically alter human MSCs derived from the synovial fluid of joints with osteoarthritis (OA) to explore the potential of reprogrammed MSCs for repairing articular cartilage. Materials and Methods MSCs isolated from the synovial fluid of three patients’ OA knees (Pa-MSCs) were reprogrammed through overexpression of pluripotency factors and then induced for differentiation to establish reprogrammed MSC (Re-MSC) lines. We compared the in vitro growth characteristics, chondrogenesis for articular cartilage chondrocytes, and immunomodulatory capacity. We also evaluated the capability of Re-MSCs to repair articular cartilage damage in an animal model with spontaneous OA. Results Our results showed that Re-MSCs increased the in vitro proliferative capacity and improved chondrogenic differentiation toward articular cartilage-like chondrocyte phenotypes with increased THBS4 and SIX1 and decreased ALPL and COL10A1, compared to Pa-MSCs. In addition, Re-MSC-derived chondrocytes expressing elevated COL2A and COL2B were more mature than parental cell-derived ones. The enhancement in chondrogenesis of Re-MSC involves the upregulation of sonic hedgehog signaling. Moreover, Re-MSCs improved the repair of articular cartilage in an animal model of spontaneous OA. Conclusions Epigenetic reprogramming promotes MSCs harvested from OA patients to increase phenotypic characteristics and gain robust functions. In addition, Re-MSCs acquire an enhanced potential for articular cartilage repair. Our study here demonstrates that the reprogramming strategy provides a potential solution to the challenge of variation in MSC quality.

scholarly journals Natural Type II Collagen Hydrogel, Fibrin Sealant, and Adipose-Derived Stem Cells as a Promising Combination for Articular Cartilage Repair

Cartilage ◽  
2016 ◽  
Vol 8 (4) ◽  
pp. 439-443 ◽  
Author(s):  
Mariana Lazarini ◽  
Pedro Bordeaux-Rego ◽  
Renata Giardini-Rosa ◽  
Adriana S. S. Duarte ◽  
Mariana Ozello Baratti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Fibrin Sealant ◽  
Type Ii Collagen ◽  
Adipose Derived Stem Cells ◽  
Type Ii ◽  
Articular Cartilage Repair ◽  
Collagen Hydrogel

Objective Articular cartilage is an avascular tissue with limited ability of self-regeneration and the current clinical treatments have restricted capacity to restore damages induced by trauma or diseases. Therefore, new techniques are being tested for cartilage repair, using scaffolds and/or stem cells. Although type II collagen hydrogel, fibrin sealant, and adipose-derived stem cells (ASCs) represent suitable alternatives for cartilage formation, their combination has not yet been investigated in vivo for focal articular cartilage defects. We performed a simple experimental procedure using the combination of these 3 compounds on cartilage lesions of rabbit knees. Design The hydrogel was developed in house and was first tested in vitro for chondrogenic differentiation. Next, implants were performed in chondral defects with or without ASCs and the degree of regeneration was macroscopically and microscopically evaluated. Results Production of proteoglycans and the increased expression of collagen type II (COL2α1), aggrecan (ACAN), and sex-determining region Y-box 9 (SOX9) confirmed the chondrogenic character of ASCs in the hydrogel in vitro. Importantly, the addition of ASC induced a higher overall repair of the chondral lesions and a better cellular organization and collagen fiber alignment compared with the same treatment without ASCs. This regenerating tissue also presented the expression of cartilage glycosaminoglycan and type II collagen. Conclusions Our results indicate that the combination of the 3 compounds is effective for articular cartilage repair and may be of future clinical interest.

scholarly journals Nanopolymers Delivery of the Bone Morphogenetic Protein-4 Plasmid to Mesenchymal Stem Cells Promotes Articular Cartilage Repair In Vitro and In Vivo

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Junjun Shi ◽  
Xin Zhang ◽  
Yanbin Pi ◽  
Jingxian Zhu ◽  
Chunyan Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Viral Vectors ◽  
Control Group ◽  
Articular Cartilage Repair ◽  
Experimental Group

The clinical application of viral vectors for gene therapy is limited for biosafety consideration. In this study, to promote articular cartilage repair, poly (lactic-co glycolic acid) (PLGA) nanopolymers were used as non-viral vectors to transfect rabbit mesenchymal stem cells (MSCs) with the pDC316-BMP4-EGFP plasmid. The cytotoxicity and transfection efficiency in vitro were acceptable measuring by CCK-8 and flow cytometry. After transfection, Chondrogenic markers (mRNA of Col2a1, Sox9, Bmp4, and Agg) of experimental cells (MSCs being transfected with BMP-4 plasmid by PLGA nanopolymers) were increased more than those of control cells (MSCs being transfected with naked BMP-4 plasmid alone). In vivo study, twelve rabbits (24 knees) with large full thickness articular cartilage defects were randomly divided into the experimental group (MSCs being transfected with BMP-4 plasmid by PLGA nanopolymers) and the control group (MSCs being transfected with naked BMP-4 plasmid). The experimental group showed better regeneration than the control group 6 and 12 weeks postoperatively. Hyaline-like cartilage formed at week 12 in the experimental group, indicating the local delivery of BMP-4 plasmid to MSCs by PLGA nanopolymers improved articular cartilage repair significantly. PLGA nanopolymers could be a promising and effective non-viral vector for gene therapy in cartilage repair.

Autologous Chondrocytes GrownIn Vitroon a Collagen Scaffold for Subsequent Articular Cartilage Repair

2005 ◽  
Vol 28 (2) ◽  
pp. 57-61
Author(s):  
Paola Narducci ◽  
Giovanna Baldini ◽  
Vittorio Grill ◽  
Vanessa Nicolin ◽  
Renato Bareggi ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Collagen Scaffold ◽  
Articular Cartilage Repair

scholarly journals A comparison of BMP2 delivery by coacervate and gene therapy for promoting human muscle-derived stem cell-mediated articular cartilage repair

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Xueqin Gao ◽  
Haizi Cheng ◽  
Hassan Awada ◽  
Ying Tang ◽  
Sarah Amra ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Raman Spectroscopy ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Chondrogenic Differentiation ◽  
Human Muscle ◽  
Articular Cartilage Repair ◽  
Sustain Release

Abstract Background Osteoarthritis and cartilage injury treatment is an unmet clinical need. Therefore, development of new approaches to treat these diseases is critically needed. Previous work in our laboratory has shown that murine muscle-derived stem cells (MDSCs) can efficiently repair articular cartilage in an osteochondral and osteoarthritis model. However, the cartilage repair capacity of human muscle-derived stem cells has not been studied which prompt this study. Method In this study, we tested the in vitro chondrogenesis ability of six populations of human muscle-derived stem cells (hMDSCs), before and after lenti-BMP2/GFP transduction using pellet culture and evaluated chondrogenic differentiation of via histology and Raman spectroscopy. We further compared the in vivo articular cartilage repair of hMDSCs stimulated with BMP2 delivered through coacervate sustain release technology and lenti-viral gene therapy-mediated gene delivery in a monoiodoacetate (MIA)-induced osteoarthritis (OA) model. We used microCT and histology to evaluate the cartilage repair. Results We observed that all hMDSCs were able to undergo chondrogenic differentiation in vitro. As expected, lenti-BMP2/GFP transduction further enhanced the chondrogenic differentiation capacities of hMDSCs, as confirmed by Alcian blue and Col2A1staining as well as Raman spectroscopy analysis. We observed through micro-CT scanning, Col2A1 staining, and histological analyses that delivery of BMP2 with coacervate could achieve a similar articular cartilage repair to that mediated by hMDSC-LBMP2/GFP. We also found that the addition of soluble fms-like tyrosine kinase-1 (sFLT-1) protein further improved the regenerative potential of hMDSCs/BMP2 delivered through the coacervate sustain release technology. Donor cells did not primarily contribute to the repaired articular cartilage since most of the repair cells are host derived as indicated by GFP staining. Conclusions We conclude that the delivery of hMDSCs and BMP2 with the coacervate technology can achieve a similar cartilage repair relative to lenti-BMP2/GFP-mediated gene therapy. The use of coacervate technology to deliver BMP2/sFLT1 with hMDSCs for cartilage repair holds promise for possible clinical translation into an effective treatment modality for osteoarthritis and traumatic cartilage injury.

PVA‐Based Hydrogels: Promising Candidates for Articular Cartilage Repair

2021 ◽  
pp. 2100147
Author(s):  
Yuru Chen ◽  
Jian Song ◽  
Song Wang ◽  
Weiqiang Liu
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Articular Cartilage Repair

Articular Cartilage Repair Strategies in the Ankle Joint

2007 ◽  
pp. 283-309 ◽  
Author(s):  
Monika Volesky ◽  
Timothy Charlton ◽  
Jonathan T. Deland
Keyword(s):  
Articular Cartilage ◽  
Ankle Joint ◽  
Cartilage Repair ◽  
Articular Cartilage Repair

BMPs in articular cartilage repair

2002 ◽  
pp. 249-262 ◽  
Author(s):  
Mislav Jelic ◽  
Marko Pecina ◽  
Miroslav Haspl ◽  
Anton Brkic ◽  
Slobodan Vukicevic
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Articular Cartilage Repair
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