Synchronized integrin engagement and chemokine activation is crucial in neutrophil extracellular trap–mediated sterile inflammation

Blood ◽  
2014 ◽  
Vol 123 (16) ◽  
pp. 2573-2584 ◽  
Author(s):  
Jan Rossaint ◽  
Jan M. Herter ◽  
Hugo Van Aken ◽  
Markus Napirei ◽  
Yvonne Döring ◽  
...  
Keyword(s):  
Neutrophil Recruitment ◽  
Sterile Inflammation ◽  
Neutrophil Extracellular Trap ◽  
Key Points ◽  
Extracellular Trap ◽  
Stimulation Of

Key PointsNET formation is required for neutrophil recruitment during sterile inflammation. Platelet-induced NET formation requires stimulation of neutrophils by platelet chemokines and outside-in signaling via the integrin Mac-1.

scholarly journals P-selectin promotes neutrophil extracellular trap formation in mice

Blood ◽  
2015 ◽  
Vol 126 (2) ◽  
pp. 242-246 ◽  
Author(s):  
Julia Etulain ◽  
Kimberly Martinod ◽  
Siu Ling Wong ◽  
Stephen M. Cifuni ◽  
Mirta Schattner ◽  
...  
Keyword(s):  
Neutrophil Extracellular Trap ◽  
Trap Formation ◽  
Key Points ◽  
Extracellular Trap ◽  
Soluble P

Key Points NET formation is stimulated by platelet or soluble P-selectin.

Potent irreversible P2Y12 inhibition does not reduce LPS-induced coagulation activation in a randomized, double-blind, placebo-controlled trial

2016 ◽  
Vol 130 (6) ◽  
pp. 433-440 ◽  
Author(s):  
Christian Schoergenhofer ◽  
Michael Schwameis ◽  
Eva-Luise Hobl ◽  
Cihan Ay ◽  
Nigel S. Key ◽  
...  
Keyword(s):  
Controlled Trial ◽  
Sterile Inflammation ◽  
P2y12 Receptor ◽  
Neutrophil Extracellular Trap ◽  
Coagulation Activation ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Trap Formation ◽  
Extracellular Trap ◽  
Receptor Inhibitor

Intake of prasugrel, a strong P2Y12 receptor inhibitor, does not affect LPS-induced activation of coagulation. Sterile inflammation by LPS increases histone-complexed DNA, a surrogate parameter of neutrophil extracellular trap formation.

scholarly journals Hydroxyethyl starch 130/0.4 decreases inflammation, neutrophil recruitment, and neutrophil extracellular trap formation

2015 ◽  
Vol 114 (3) ◽  
pp. 509-519 ◽  
Author(s):  
J. Rossaint ◽  
C. Berger ◽  
F. Kraft ◽  
H. Van Aken ◽  
N. Giesbrecht ◽  
...  
Keyword(s):  
Hydroxyethyl Starch ◽  
Neutrophil Recruitment ◽  
Neutrophil Extracellular Trap ◽  
Trap Formation ◽  
Extracellular Trap

scholarly journals IgA Complexes Induce Neutrophil Extracellular Trap Formation More Potently Than IgG Complexes

2022 ◽  
Vol 12 ◽  
Author(s):  
Anna-Katharina Gimpel ◽  
Antonio Maccataio ◽  
Harald Unterweger ◽  
Maria V. Sokolova ◽  
Georg Schett ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Immune Complexes ◽  
Driving Force ◽  
Neutrophil Extracellular Trap ◽  
Autologous Serum ◽  
Collateral Damage ◽  
Potent Inducer ◽  
Unstimulated Control ◽  
Extracellular Trap ◽  
Stimulation Of

Neutrophil extracellular trap (NET) formation is a powerful instrument to fight pathogens, but may induce collateral damage in the affected tissues. Besides pathogen-derived factors, immune complexes are potent inducers of NET formation. Neutrophils express IgA and IgG specific Fc receptors (FcRs) and therefore respond to complexed IgA and IgG. Especially in the context of autoimmune diseases, IgA and IgG immune complexes have been shown to trigger NET formation, a process that putatively contributes to disease severity. However, it is of question if both antibody classes stimulate neutrophils to the same extent. In this study, we compared the capability of IgA and IgG complexes formed by heat aggregation to induce NET formation. While stimulation of neutrophils with IgA complexes robustly induced NET formation, complexed IgG only marginally increased the amount of NETs compared to the unstimulated control. Mixing IgA with IgG before heat aggregation did not increase the effect of complexed IgA on neutrophils. By contrast, the presence of IgG complexes seemed to disturb neutrophil stimulation by IgA complexes. The capacity of complexed IgG to induce NET formation could not be increased by the addition of autologous serum or the removal of terminal sialic acid in the Fc glycan. Together, our data show that IgA is a much more potent inducer of NET formation than IgG. IgA may thus be the main driving force in (auto)immune complex-mediated NET formation.

scholarly journals IL-33 exacerbates liver sterile inflammation by amplifying neutrophil extracellular trap formation

2018 ◽  
Vol 68 (1) ◽  
pp. 130-139 ◽  
Author(s):  
Hamza O. Yazdani ◽  
Hui-Wei Chen ◽  
Samer Tohme ◽  
Sheng Tai ◽  
Dirk J. van der Windt ◽  
...  
Keyword(s):  
Sterile Inflammation ◽  
Neutrophil Extracellular Trap ◽  
Trap Formation ◽  
Extracellular Trap
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