scholarly journals Effect of cyanide on NADPH oxidation by granules from human polymorphonuclear leukocytes

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 445-454
Author(s):  
LR DeChatelet ◽  
LC McPhail ◽  
PS Shirley
Keyword(s):  
Horseradish Peroxidase ◽  
Oxygen Uptake ◽  
Polymorphonuclear Leukocytes ◽  
Hexose Monophosphate ◽  
Chain Reaction ◽  
Human Polymorphonuclear Leukocyte ◽  
Nadph Oxidation ◽  
Human Polymorphonuclear Leukocytes ◽  
Generating System ◽  
Stimulation Of

Cyanide has been shown to stimulate both oxygen uptake and hexose monophosphate shunt activity in phagocytizing human polymorphonuclear leukocytes. It also stimulates the oxidation of NADPH by a particulate fraction derived from phagocytizing cells. This stimulation of NADPH oxidase is not observed in the presence of exogenous Mn2+. Studies with purified enzymes have shown that CN- also stimulates NADPH oxidation by horseradish peroxidase or lactoperoxidase, suggesting that the respiratory burst might be initiated by activation of a peroxidase-like enzyme in the human polymorphonuclear leukocyte. Based on studies of others, however, it does not appear as though the enzyme is identical to myeloperoxidase. The mechanism of the CN- stimulation appears to involve an oxidatic chain reaction, since it stimulates markedly NADPH oxidation in the presence of an artificial superoxide-generating system.

scholarly journals Effect of cyanide on NADPH oxidation by granules from human polymorphonuclear leukocytes

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 445-454 ◽  
Author(s):  
LR DeChatelet ◽  
LC McPhail ◽  
PS Shirley
Keyword(s):  
Horseradish Peroxidase ◽  
Oxygen Uptake ◽  
Polymorphonuclear Leukocytes ◽  
Hexose Monophosphate ◽  
Chain Reaction ◽  
Human Polymorphonuclear Leukocyte ◽  
Nadph Oxidation ◽  
Human Polymorphonuclear Leukocytes ◽  
Generating System ◽  
Stimulation Of

Abstract Cyanide has been shown to stimulate both oxygen uptake and hexose monophosphate shunt activity in phagocytizing human polymorphonuclear leukocytes. It also stimulates the oxidation of NADPH by a particulate fraction derived from phagocytizing cells. This stimulation of NADPH oxidase is not observed in the presence of exogenous Mn2+. Studies with purified enzymes have shown that CN- also stimulates NADPH oxidation by horseradish peroxidase or lactoperoxidase, suggesting that the respiratory burst might be initiated by activation of a peroxidase-like enzyme in the human polymorphonuclear leukocyte. Based on studies of others, however, it does not appear as though the enzyme is identical to myeloperoxidase. The mechanism of the CN- stimulation appears to involve an oxidatic chain reaction, since it stimulates markedly NADPH oxidation in the presence of an artificial superoxide-generating system.

scholarly journals Hydrogen peroxide production and killing of Staphylococcus aureus by human polymorphonuclear leukocytes

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 437-444 ◽  
Author(s):  
MF Tsan ◽  
KH Douglass ◽  
PA McIntyre
Keyword(s):  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Horseradish Peroxidase ◽  
Polymorphonuclear Leukocytes ◽  
H2o2 Production ◽  
Intracellular Killing ◽  
Hydrogen Peroxide Production ◽  
Production Of Hydrogen ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

Abstract The effects of bacterial neuraminidase on production of hydrogen peroxide (H2O2) and killing of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) were studied. The concentration of H2O2 was measured by the disappearance of scopoletin fluorescence in the presence of horseradish peroxidase. The results indicated that desialylation of human PMN inhibited the stimulation of H2O2 production during phagocytosis. It also markedly impaired the killing of S. aureus. Impaired killing of S. aureus by desialylated PMN was due to impaired intracellular killing rather than defective phagocytosis.

scholarly journals Hydrogen peroxide production and killing of Staphylococcus aureus by human polymorphonuclear leukocytes

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 437-444
Author(s):  
MF Tsan ◽  
KH Douglass ◽  
PA McIntyre
Keyword(s):  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Horseradish Peroxidase ◽  
Polymorphonuclear Leukocytes ◽  
H2o2 Production ◽  
Intracellular Killing ◽  
Hydrogen Peroxide Production ◽  
Production Of Hydrogen ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

The effects of bacterial neuraminidase on production of hydrogen peroxide (H2O2) and killing of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) were studied. The concentration of H2O2 was measured by the disappearance of scopoletin fluorescence in the presence of horseradish peroxidase. The results indicated that desialylation of human PMN inhibited the stimulation of H2O2 production during phagocytosis. It also markedly impaired the killing of S. aureus. Impaired killing of S. aureus by desialylated PMN was due to impaired intracellular killing rather than defective phagocytosis.

scholarly journals The requirement for membrane sialic acid in the stimulation of superoxide production during phagocytosis by human polymorphonuclear leukocytes.

1976 ◽  
Vol 143 (6) ◽  
pp. 1308-1316 ◽  
Author(s):  
M Tsan ◽  
P A McIntyre
Keyword(s):  
Sialic Acid ◽  
Concanavalin A ◽  
Oxidative Metabolism ◽  
Polymorphonuclear Leukocytes ◽  
Superoxide Production ◽  
Hexose Monophosphate ◽  
Latex Particles ◽  
Hexose Monophosphate Shunt ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

The effect of desialylation on phagocytosis of latex particles and oxidative metabolism of human polymorphonuclear leukocytes was studied. Removal of 20% total leukocyte sialic acid by bacterial neuraminidase had no effect on phagocytosis of latex particles and phagocytosis-associated activation of hexose monophosphate shunt in human polymorphonuclear leukocytes. In contrast, desialylation prevented the stimulation of superoxide production either by phagocytosis or by concanavalin A. It is concluded that membrane sialic acid is essential for the stimulation of superoxide production by human polymorphonuclear leukocytes.

scholarly journals Stimulation of the hexose monophosphate shunt independent of hydrogen peroxide and superoxide production in rabbit alveolar macrophages during phagocytosis

Blood ◽  
1977 ◽  
Vol 50 (5) ◽  
pp. 935-945 ◽  
Author(s):  
MF Tsan
Keyword(s):  
Alveolar Macrophages ◽  
Polymorphonuclear Leukocytes ◽  
Light Emission ◽  
Cell Types ◽  
H2o2 Production ◽  
Hexose Monophosphate ◽  
Latex Particles ◽  
Hexose Monophosphate Shunt ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

Abstract Phagocytosis and oxidative metabolism of human polymorphonuclear leukocytes (PMN) and rabbit alveolar macrophages (AM) were studied. Human PMN ingested a mean of 12 polyvinyl toluene latex particles (2 micrometer in diameter) per cell. There was stimulation of O2- and H2O2 production, light emission, and activation of the hexose monophosphate shunt during phagocytosis by human PMN. Rabbit AM ingested 51 latex particles (2 micrometer in diameter) per cell. There was no stimulation of the production of O2- and H2O2 or light emission associated with phagocytosis by rabbit AM, while the hexose monophosphate shunt was activated. Similar metabolic changes were obtained in both cell types when opsonized zymosan was used as phagocytic particles. 1-14C- glucoseoxidation was stimulated by H2O2 and methylene blue in both resting human PMN and rabbit AM. It is concluded that activation of the hexose monophosphate shunt in rabbit AM during phagocytosis is independent of O2- and H2O2 production.

scholarly journals Stimulation of the hexose monophosphate shunt independent of hydrogen peroxide and superoxide production in rabbit alveolar macrophages during phagocytosis

Blood ◽  
1977 ◽  
Vol 50 (5) ◽  
pp. 935-945
Author(s):  
MF Tsan
Keyword(s):  
Alveolar Macrophages ◽  
Polymorphonuclear Leukocytes ◽  
Light Emission ◽  
Cell Types ◽  
H2o2 Production ◽  
Hexose Monophosphate ◽  
Latex Particles ◽  
Hexose Monophosphate Shunt ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

Phagocytosis and oxidative metabolism of human polymorphonuclear leukocytes (PMN) and rabbit alveolar macrophages (AM) were studied. Human PMN ingested a mean of 12 polyvinyl toluene latex particles (2 micrometer in diameter) per cell. There was stimulation of O2- and H2O2 production, light emission, and activation of the hexose monophosphate shunt during phagocytosis by human PMN. Rabbit AM ingested 51 latex particles (2 micrometer in diameter) per cell. There was no stimulation of the production of O2- and H2O2 or light emission associated with phagocytosis by rabbit AM, while the hexose monophosphate shunt was activated. Similar metabolic changes were obtained in both cell types when opsonized zymosan was used as phagocytic particles. 1-14C- glucoseoxidation was stimulated by H2O2 and methylene blue in both resting human PMN and rabbit AM. It is concluded that activation of the hexose monophosphate shunt in rabbit AM during phagocytosis is independent of O2- and H2O2 production.

Gonococci-human polymorphonuclear leukocyte interactions: metabolic studies associated with attachment and ingestion

1980 ◽  
Vol 28 (3) ◽  
pp. 991-1000
Author(s):  
A G Krieger ◽  
N L Schiller ◽  
R B Roberts
Keyword(s):  
Oxidative Metabolism ◽  
Polymorphonuclear Leukocytes ◽  
Surface Membrane ◽  
Optimal Conditions ◽  
Human Polymorphonuclear Leukocyte ◽  
Respiratory Inhibitors ◽  
Phase Type ◽  
Human Polymorphonuclear Leukocytes ◽  
Dose Dependent

Utilizing monolayers of human polymorphonuclear leukocytes, optimal conditions for attachment and ingestion of Neisseria gonorrhoeae were determined. Both attachment and ingestion were optimal at 36 degrees C when a bacteria-leukocyte ratio of 100:1 was employed. After 30 min of incubation, log-phase viable type 2 gonococci were attached to 90% of leukocytes, whereas log-phase viable type 4 gonococci were ingested by 80 to 90% of cells. Respiratory inhibitors had no effect on attachment or ingestion, whereas glycolytic inhibitors blocked ingestion but did not affect attachment of gonocci to the leukocyte surface. Inhibition was dose dependent and partially reversible. The oxidative metabolism of leukocytes with gonococci attached or ingested was also examined. Attachment of log-phase type 2 gonococci stimulated a minimal increase in glucose oxidation and oxygen consumption by leukocytes in contrast to marked increases by leukocytes that had ingested viable type 4 or heat-killed typed 2 organisms. These results demonstrate that attachment of log-phase type 2 gonococci to the surface membrane does not stimulate significant leukocyte oxidative metabolism nor initiate the phagocytic process.

Sign in / Sign up

Export Citation Format

Share Document