scholarly journals In vitro cell growth in neonates with Down's syndrome and transient myeloproliferative disorder

Blood ◽  
1981 ◽  
Vol 58 (4) ◽  
pp. 675-677
Author(s):  
JF Denegri ◽  
PC Rogers ◽  
KW Chan ◽  
J Sadoway ◽  
JW Thomas
Keyword(s):  
Cell Growth ◽  
Down's Syndrome ◽  
Down’S Syndrome ◽  
Myeloproliferative Disorders ◽  
Maternal Serum ◽  
Blast Cell ◽  
Congenital Leukemia ◽  
Myeloproliferative Syndrome ◽  
Serum Inhibitor

Bone marrow and peripheral blood cells from three newborns with Down's syndrome and transient myeloproliferative disorders were cultured in vitro. In the methylcellulose semiliquid system, normal colony formation with maturation and differentiation into granulocytes and monocyte-macrophages were observed in all three patients. This is different from the growth pattern usually seen in acute nonlymphocytic leukemia. A maternal serum inhibitor of both normal allogeneic GM-CFU and blast cell growth was also demonstrated, but its role in pathogenesis is uncertain. Normal in vitro granulopoiesis may help to differentiate the transient myeloproliferative syndrome from congenital leukemia in newborns with Down's syndrome.

scholarly journals In vitro cell growth in neonates with Down's syndrome and transient myeloproliferative disorder

Blood ◽  
1981 ◽  
Vol 58 (4) ◽  
pp. 675-677 ◽  
Author(s):  
JF Denegri ◽  
PC Rogers ◽  
KW Chan ◽  
J Sadoway ◽  
JW Thomas
Keyword(s):  
Cell Growth ◽  
Down's Syndrome ◽  
Down’S Syndrome ◽  
Myeloproliferative Disorders ◽  
Maternal Serum ◽  
Blast Cell ◽  
Congenital Leukemia ◽  
Myeloproliferative Syndrome ◽  
Serum Inhibitor

Abstract Bone marrow and peripheral blood cells from three newborns with Down's syndrome and transient myeloproliferative disorders were cultured in vitro. In the methylcellulose semiliquid system, normal colony formation with maturation and differentiation into granulocytes and monocyte-macrophages were observed in all three patients. This is different from the growth pattern usually seen in acute nonlymphocytic leukemia. A maternal serum inhibitor of both normal allogeneic GM-CFU and blast cell growth was also demonstrated, but its role in pathogenesis is uncertain. Normal in vitro granulopoiesis may help to differentiate the transient myeloproliferative syndrome from congenital leukemia in newborns with Down's syndrome.

Stability of maternal serum free β-hCG following whole blood sample transit: First trimester Down’s syndrome screening in Scotland

2021 ◽  
pp. 000456322110452
Author(s):  
Angela Ballantyne ◽  
Lorna Rashid ◽  
Rebecca Pattenden
Keyword(s):  
Transit Time ◽  
Down's Syndrome ◽  
Down’S Syndrome ◽  
First Trimester ◽  
Maternal Serum ◽  
Dependent Manner ◽  
Serum Free ◽  
Β Hcg ◽  
In Transit ◽  
The Impact

Background Maternal serum free beta human chorionic gonadotrophin (free β-hCG) is used as a biomarker in first trimester screening for fetal Down’s syndrome. Production of free β-hCG can occur in vitro in a time- and temperature-dependent manner; thus, the current Scottish screening protocol states samples must be received by the laboratory within 72 h. To assess the validity of the protocol, an audit was conducted to determine the impact of transit time on maternal serum free β-hCG multiple of median (MoM) values in the Scottish screened population. Methods Corrected MoM values from antenatal screening carried out over one year (April 2017 to March 2018) were stratified according to sample transit time and compared. To investigate possible environmental temperature effects, the data were split according to season and maternal serum free β-hCG concentrations from summer and winter compared. Results Of the 28,368 samples included in the study, 24,368 were received on the day of phlebotomy or after one day in transit. Only 1.5% of samples were received after 3 days in transit. The difference in maternal serum free β-hCG MoM values due to transit time was not significant. No statistical difference was found between maternal serum free β-hCG concentrations from samples collected in summer and winter months. Conclusion The current sample receipt protocol in use by the Scottish Down’s syndrome screening programme is fit for purpose.

Low second trimester maternal serum unconjugated oestriol in pregnancies with Down's syndrome

1988 ◽  
Vol 95 (4) ◽  
pp. 330-333 ◽  
Author(s):  
J. A. CANICK ◽  
G. J. KNIGHT ◽  
G. E. PALOMAK1 ◽  
J. E. HADDOW ◽  
H. S. CUCKLE ◽  
...  
Keyword(s):  
Down's Syndrome ◽  
Down’S Syndrome ◽  
Maternal Serum ◽  
Second Trimester

scholarly journals Differentiation of blast cells from a Down's syndrome patient with transient myeloproliferative disorder

Blood ◽  
1987 ◽  
Vol 69 (2) ◽  
pp. 508-512
Author(s):  
J Suda ◽  
M Eguchi ◽  
Y Akiyama ◽  
Y Iwama ◽  
T Furukawa ◽  
...  
Keyword(s):  
Down's Syndrome ◽  
Down’S Syndrome ◽  
Colony Growth ◽  
Myeloproliferative Disorder ◽  
In Vitro Proliferation ◽  
Gm Csf ◽  
Blast Cells ◽  
Macrophage Colony ◽  
Male Neonate

A male neonate with Down's syndrome and congenital myeloproliferative disorder was studied. His blood picture showed the unique coexistence of leukocytosis with matured cells and a large number of blast cells. The in vitro proliferation and differentiation of blast cells into various lineages in the presence of phytohemagglutinin-stimulated leukocyte conditioned medium (PHA-LCM) was examined by using a liquid culture and a methylcellulose culture system. The differentiation of blast cells into myeloid cells was confirmed by specific cytochemical stainings, electron microscopy, and an immunologic study. No specific factors in the plasma of the patient promoted the proliferation or differentiation of blast cells. The cellular composition of colonies grown in methylcellulose culture from single blast cells was studied by a micromanipulation technique. High plating efficiency was observed. Of 136 cultures, 78 showed colony growth. Half of the blast cells were colony-forming cells that could proliferate and differentiate into basophils, neutrophils, eosinophils, macrophages, and erythrocytes in the presence of PHA-LCM. Using the blast cells with a high differentiation capacity to the basophil pathway, we studied the effect of recombinant granulocyte-macrophage colony-stimulating factor (GM- CSF). Recombinant GM-CSF support neutrophils, eosinophils, and macrophages but not typical basophils. These findings of the cell differentiation of blast cells into various kinds of cells in vitro were in agreement with the finding of neutrophilia, eosinophilia, basophilia, and thrombocythemia in this patient.

scholarly journals Clonal analysis of basophil differentiation in bone marrow cultures from a Down's syndrome patient with megakaryoblastic leukemia

Blood ◽  
1985 ◽  
Vol 66 (6) ◽  
pp. 1278-1283
Author(s):  
T Suda ◽  
J Suda ◽  
Y Miura ◽  
Y Hayashi ◽  
M Eguchi ◽  
...  
Keyword(s):  
Single Cells ◽  
Down's Syndrome ◽  
Calcium Ionophore ◽  
Down’S Syndrome ◽  
Colony Growth ◽  
Leukemic Cells ◽  
Cytologic Examination ◽  
Megakaryoblastic Leukemia

We present the in vitro differentiation of marrow cells from a patient with Down's syndrome accompanied by megakaryoblastic leukemia into basophils in the presence of phytohemagglutinin-stimulated leukocyte conditioned medium, using a liquid culture and methylcellulose culture system. Identification of basophils was established by metachromatic staining with toluidine blue, transmission electron microscopy, and the presence of histamine. However, these basophils did not release histamine in response to calcium ionophore or chemotactic peptide. Samples from suspension cultures that contained 90% basophils showed chromosomal markers characteristic of leukemic cells (48, XY, +11, +21, t(1;15)) in all examined mitoses. The cellular composition of leukemic colonies grown in methylcellulose culture from single cells was studied using the micromanipulation technique. High plating efficiency and extreme predominance of basophil colonies were observed. In a total 137 cultures, 79 revealed colony growth. Of 59 colonies that were analyzed by cytologic examination, 46 were pure basophil colonies. These basophil colonies showed disperse morphology, similar to that of a normal basophil colony. The clonality of the basophil colonies and skewing of lineage expression were documented from leukemic single-cell cultures. These data showed that leukemic cells have the capacity for differentiation into some lineages that are not expressed in vivo.

Low maternal serum alpha-fetoprotein at 10 weeks gestation and fetal Down's syndrome

1989 ◽  
Vol 96 (4) ◽  
pp. 499-500 ◽  
Author(s):  
A. Mantingh ◽  
J. Marrink ◽  
B. Wolf ◽  
A. S. P. M. Breed ◽  
J. R. Beekhuis ◽  
...  
Keyword(s):  
Down's Syndrome ◽  
Down’S Syndrome ◽  
Maternal Serum ◽  
Alpha Fetoprotein ◽  
Serum Alpha Fetoprotein
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