A cell line secreting stimulating factors for CFU-GEMM culture

Blood ◽  
1984 ◽  
Vol 64 (1) ◽  
pp. 152-155 ◽  
Author(s):  
CD Myers ◽  
FE Katz ◽  
G Joshi ◽  
JL Millar
Keyword(s):  
Stem Cell ◽  
Cell Line ◽  
Conditioned Medium ◽  
Carcinoma Cell ◽  
Hemopoietic Stem Cell ◽  
Growth Requirements ◽  
Bladder Carcinoma Cell Line ◽  
A Cell ◽  
Hemopoietic Progenitor

The multipotent hemopoietic stem cell has fastidious growth requirements in vitro. Traditionally, phytohemagglutinin-stimulated leukocyte conditioned medium has been used to supply the undefined growth factors required for culture of the human multipotent hemopoietic progenitor. We describe the use of medium conditioned by the bladder carcinoma cell line, 5637, to replace PHA-LCM in CFU-GEMM cultures and show that the properties of this conditioned medium closely mimic those of PHA-LCM in two separate CFU-GEMM culture systems.

scholarly journals A cell line secreting stimulating factors for CFU-GEMM culture

Blood ◽  
1984 ◽  
Vol 64 (1) ◽  
pp. 152-155 ◽  
Author(s):  
CD Myers ◽  
FE Katz ◽  
G Joshi ◽  
JL Millar
Keyword(s):  
Stem Cell ◽  
Cell Line ◽  
Conditioned Medium ◽  
Carcinoma Cell ◽  
Hemopoietic Stem Cell ◽  
Growth Requirements ◽  
Bladder Carcinoma Cell Line ◽  
A Cell ◽  
Hemopoietic Progenitor

Abstract The multipotent hemopoietic stem cell has fastidious growth requirements in vitro. Traditionally, phytohemagglutinin-stimulated leukocyte conditioned medium has been used to supply the undefined growth factors required for culture of the human multipotent hemopoietic progenitor. We describe the use of medium conditioned by the bladder carcinoma cell line, 5637, to replace PHA-LCM in CFU-GEMM cultures and show that the properties of this conditioned medium closely mimic those of PHA-LCM in two separate CFU-GEMM culture systems.

scholarly journals The effect of strontium ranelate on titanium particle-induced periprosthetic osteolysis regulated by WNT/β-catenin signaling in vivo and in vitro

2021 ◽  
Vol 41 (1) ◽  
Author(s):  
Bolun Wang ◽  
Haohui Guo ◽  
Tianxiang Geng ◽  
Kening Sun ◽  
Liang Zhang ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Aseptic Loosening ◽  
Cell Line ◽  
Conditioned Medium ◽  
Strontium Ranelate ◽  
Cell Model ◽  
Periprosthetic Osteolysis ◽  
A Cell

Abstract Aseptic loosening following periprosthetic osteolysis is the primary complication that limits the lifetime of total joint arthroplasty (TJA). The wear particles trigger a chronic inflammation response in the periprosthetic tissue and turn over the bone balance to bone resorption. The present study aimed to investigate the possible effect and mechanism of strontium ranelate (SR), a clinically safe drug for osteoporosis, on particle-induced periprosthetic osteolysis. Thirty-six female C57BL/6j mice underwent tibial Ti-nail implantation to establish an animal model of aseptic loosening. After 12 weeks, micro-CT results showed that strontium ranelate could inhibit periprosthetic bone resorption. In vitro, Ti particles were used to stimulate RAW264.7 cell line to collect conditioned medium, and co-culture MC3T3-E1 cell line with conditioned medium to establish a cell model of aseptic loosening. The results of alkaline phosphatase (ALP) detection, immunofluorescence, and flow cytometry demonstrated that strontium ranelate could regulate the expression of OPG/RANKL, promote differentiation and mineralization, and inhibit apoptosis in osteoblasts. Moreover, we revealed that SR’s exerted its therapeutic effect by down-regulating sclerostin, thereby activating the Wnt/β-catenin signal pathway. Therefore, this research suggests that strontium ranelate could be a potential drug for the prevention and treatment of particle-induced aseptic loosening post-TJA.

scholarly journals Identification of Cells in Primate Bone Marrow Resembling the Hemopoietic Stem Cell in the Mouse

Blood ◽  
1973 ◽  
Vol 42 (2) ◽  
pp. 195-208 ◽  
Author(s):  
K. A. Dicke ◽  
M. J. van Noord ◽  
B. Maat ◽  
U. W. Schaefer ◽  
D. W. van Bekkum
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Staining Method ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Hemopoietic Stem Cell ◽  
Vitro Assay ◽  
A Cell ◽  
Unit Culture

Abstract The colony-forming unit culture (CFU-C) in the thin-layer agar colony technique is considered to be representative for hemopoietic stem cells (HSC), according to our studies in mouse and monkey bone marrow. Using this in vitro assay as a guide, stem cell concentrates were prepared from monkey and human bone marrow by repeated density gradient centrifugation. The number of CFU-C could be enriched up to 70-100-fold. In such concentrated CFU-C suspensions, a cell, morphologically identical with the hemopoietic stem cell in the mouse (MSCLC, mouse stem cell-like cell) was frequently observed, using a May-Grünwald-Giemsa (MGG) staining method and electron microscope techniques. In MGG-stained preparations, the MSCLC superficially resembles the small lymphocyte; therefore, a staining method has been described, the polychrome procedure, by which both cell populations could be clearly distinguished. Since a fair correlation exists between the number of MSCLC and the number of CFU-C in a variety of primate hemopoietic suspensions, we concluded that the MSCLC might be a good candidate for being the HSC in monkeys and man.

scholarly journals Production of leukemic blast growth factor by a human bladder carcinoma cell line

Blood ◽  
1985 ◽  
Vol 66 (3) ◽  
pp. 748-751
Author(s):  
T Hoang ◽  
EA McCulloch
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Conditioned Medium ◽  
Bladder Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Leukemic Blast ◽  
Human Bladder ◽  
Complete Replacement ◽  
Bladder Carcinoma Cell Line

Circulating blast cells from the peripheral blood of acute myeloblastic leukemia patients include a subpopulation capable of colony formation in the presence of phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM). We describe the complete replacement in the blast assay of PHA-LCM by conditioned medium from a human bladder carcinoma cell line, HTB9. Both conditioned media contain a stimulator of blast cell growth that elutes as a single peak from a Sephadex G100 column with an apparent molecular weight of 30,000. It is shown that this leukemic blast growth factor is distinct from erythroid-potentiating activity (EPA) and possibly granulocyte macrophage colony-stimulating factor.

scholarly journals Production of leukemic blast growth factor by a human bladder carcinoma cell line

Blood ◽  
1985 ◽  
Vol 66 (3) ◽  
pp. 748-751 ◽  
Author(s):  
T Hoang ◽  
EA McCulloch
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Conditioned Medium ◽  
Bladder Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Leukemic Blast ◽  
Human Bladder ◽  
Complete Replacement ◽  
Bladder Carcinoma Cell Line

Abstract Circulating blast cells from the peripheral blood of acute myeloblastic leukemia patients include a subpopulation capable of colony formation in the presence of phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM). We describe the complete replacement in the blast assay of PHA-LCM by conditioned medium from a human bladder carcinoma cell line, HTB9. Both conditioned media contain a stimulator of blast cell growth that elutes as a single peak from a Sephadex G100 column with an apparent molecular weight of 30,000. It is shown that this leukemic blast growth factor is distinct from erythroid-potentiating activity (EPA) and possibly granulocyte macrophage colony-stimulating factor.

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