scholarly journals Acute lymphoblastic leukemia with unusual cytoplasmic granulation: a morphologic, cytochemical, and ultrastructural study

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 406-411 ◽  
Author(s):  
J Fradera ◽  
E Velez-Garcia ◽  
JG White
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Acute Leukemias ◽  
Ultrastructural Studies ◽  
Sudan Black B ◽  
Blast Cells ◽  
Chediak Higashi Syndrome ◽  
Schiff Reaction

Abstract The classification of the acute leukemias depends mainly on the morphologic and cytochemical evaluation of the blast forms. One of the main accepted morphologic criteria in the differentiation between acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML) is the absence of granules in the blast cells of ALL. We evaluated a patient with ALL in whom granules were present in the cytoplasm of 35% of the blast cells, as seen in AML. Cytochemical evaluation was performed, including periodic acid-Schiff reaction, Sudan black B, alpha-naphthyl acetate, alpha-naphthyl butyrate, naphthol AS-D chloroacetate, and acid phosphatase stains. The results of these studies confirmed the morphologic impression and diagnosis of ALL. Ultrastructural evaluation revealed that the granules consisted of many tiny vesicles closely packed together in a proteinaceous matrix, resembling to some extent the inclusions described in lymphocytes in the Chediak-Higashi syndrome, but clearly different. The morphologic, cytochemical, and ultrastructural studies of this unique case are presented in detail. To our knowledge, this is the first time that such granules have been described in blast cells of ALL.

scholarly journals Acute lymphoblastic leukemia with unusual cytoplasmic granulation: a morphologic, cytochemical, and ultrastructural study

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 406-411
Author(s):  
J Fradera ◽  
E Velez-Garcia ◽  
JG White
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Acute Leukemias ◽  
Ultrastructural Studies ◽  
Sudan Black B ◽  
Blast Cells ◽  
Chediak Higashi Syndrome ◽  
Schiff Reaction

The classification of the acute leukemias depends mainly on the morphologic and cytochemical evaluation of the blast forms. One of the main accepted morphologic criteria in the differentiation between acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML) is the absence of granules in the blast cells of ALL. We evaluated a patient with ALL in whom granules were present in the cytoplasm of 35% of the blast cells, as seen in AML. Cytochemical evaluation was performed, including periodic acid-Schiff reaction, Sudan black B, alpha-naphthyl acetate, alpha-naphthyl butyrate, naphthol AS-D chloroacetate, and acid phosphatase stains. The results of these studies confirmed the morphologic impression and diagnosis of ALL. Ultrastructural evaluation revealed that the granules consisted of many tiny vesicles closely packed together in a proteinaceous matrix, resembling to some extent the inclusions described in lymphocytes in the Chediak-Higashi syndrome, but clearly different. The morphologic, cytochemical, and ultrastructural studies of this unique case are presented in detail. To our knowledge, this is the first time that such granules have been described in blast cells of ALL.

The periodic acid-schiff reaction and prognosis in children with acute lymphoblastic leukemia

1979 ◽  
Vol 6 (1) ◽  
pp. 27-34 ◽  
Author(s):  
R. Beverly Raney ◽  
Robert S. Festa ◽  
Maria T. G. Waldman ◽  
David Manson ◽  
Hie-Won L. Hann
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Schiff Reaction

Periodic Acid-Schiff Stain in Childhood Acute Lymphoblastic Leukemia: Lack of Independent Prognostic Value

1985 ◽  
Vol 83 (4) ◽  
pp. 494-497
Author(s):  
Gary R. Lanham ◽  
David K. Kalwinsky ◽  
Dorothy L. Williams ◽  
Susan L. Melvin ◽  
Jerry R. Harber ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Prognostic Value ◽  
Lymphoblastic Leukemia ◽  
Periodic Acid ◽  
Childhood Acute Lymphoblastic Leukemia ◽  
Periodic Acid Schiff

scholarly journals LEUKEMIC BLAST CELLS AND CONTROVERSIES IN MODELS OF HEMATOPOIESIS

2015 ◽  
Vol 37 (1) ◽  
pp. 2-4 ◽  
Author(s):  
D F Gluzman ◽  
L M Sklyarenko ◽  
M P Zavelevich ◽  
S V Koval ◽  
T S Ivanivskaya
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Leukemic Blast ◽  
Acute Leukemias ◽  
Cell Lineages ◽  
Hematopoietic Lineage ◽  
Acute Monoblastic Leukemia ◽  
Blast Cells ◽  
The Common ◽  
Insight Into

Classical and up-to-date models of hematopoietic lineage determination are briefly reviewed with the focus on myeloid-based models challenging the existence of the common progenitor for T cells, B cells and NK cells. The analysis of immunophenotype of leukemic blast cells seems to be a promising approach for interpreting some controversies in the schemes of normal hematopoiesis. The liter ature data as well as our own findings in the patients with various types of acute leukemias are in favor of the concept postulating that common myeloid-lymphoid progenitors giving rise to T and B cell branches retain the myeloid potential. The similarity of some immunophenotypic features of blast cells in pro-B acute lymphoblastic leukemia and acute monoblastic leukemia is consistent with monocyte origin postulated in the studies of normal hematopoiesis. Study of acute leukemias may be the challenging area of research allowing for new insight into the origin of hematopoietic cell lineages.

scholarly journals A VERTICAL MICROSYSTEM FOR DISCONTINUOUS ELECTROPHORESIS OF INSECT TISSUE PROTEINS USING THIN SHEETS OF POLYACRYLAMIDE GEL

1972 ◽  
Vol 20 (5) ◽  
pp. 368-384 ◽  
Author(s):  
ANITA C. BEEN ◽  
ELLEN M. RASCH
Keyword(s):  
Periodic Acid ◽  
Polyacrylamide Gel ◽  
Thin Sheets ◽  
Periodic Acid Schiff ◽  
Sudan Black B ◽  
Tissue Extracts ◽  
Salivary Gland Cells ◽  
Schiff Reaction ◽  
Coomassie Brilliant ◽  
Nonspecific Esterases

Proteins extracted from individual pairs of salivary glands or other larval tissues of Sciara coprophila (Diptera) were separated in a vertical microsystem for discontinuous electrophoresis using thin sheets of polyacrylamide gel cast in multiple layers of varying pore size. After electrophoresis at 150 volts for 40 min, gels were stained ( a) for total proteins with Coomassie brilliant blue, ( b) for glycoproteins with the periodic acid-Schiff reaction, ( c) for lipoproteins with Sudan black B or ( d) for nonspecific esterases with fast blue RR as coupler and α-naphthol acetate as substrate. Sequential application of these reactions to individual gel sectors permitted direct comparisons of protein profiles for 15-20 different samples of tissue extracts carried on a single gel sheet in adjacent lanes and thus subjected to identical conditions of electrophoresis. Representative photographs and densitometric scans are presented to show the suitability of thin gel sheets for autoradiography and for both qualitative and quantitative evaluation of tissue-specific differences in patterns of protein banding found for salivary gland cells, the gastric ceca, or the hemolymph of individual Sciara larvae sacrificed at particular stages of fourth instar development. Innovative details of methodology are presented, including the use of a microspectrophotometer to scan electropherograms of insect proteins and several types of human blood serum.

scholarly journals Near-haploid acute lymphoblastic leukemia: a unique subgroup with a poor prognosis?

Blood ◽  
1981 ◽  
Vol 58 (1) ◽  
pp. 14-19 ◽  
Author(s):  
GM Brodeur ◽  
DL Williams ◽  
AT Look ◽  
WP Bowman ◽  
DK Kalwinsky
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Dna Content ◽  
Poor Prognosis ◽  
Lymphoblastic Leukemia ◽  
Periodic Acid ◽  
Flow Cytometric Analysis ◽  
Chromosome 21 ◽  
Periodic Acid Schiff ◽  
Flow Cytometric ◽  
Block Pattern

We describe two adolescent girls with acute lymphoblastic leukemia (ALL) whose leukemia cells were near-haploid. Their lymphoblasts stained in a block pattern with periodic acid Schiff and had “common ALL” surface markers confirmed by indirect immunofluorescence. Each patient had two populations of blasts, one near-haploid and one hyperdiploid, which was an exact doubling of the near-haploid karyotype. The first patient had a predominant population of cells with 26 chromosomes and a few with 52, while the second had a predominance of cells with 56 and a minority with 28. Flow cytometric analysis of DNA content initially detected the minor near-haploid population in the second patient, which was confirmed later by cytogenetic review of the marrow sample. In addition to our two patients, only four patients have been reported with near-haploid ALL. Of these six, five were girls, five were adolescents, and five had short survivals (median, 10 mo). All six had disomy of chromosome 21 with or without disomy for chromosomes 10, 14, 18, or X (four patients each). Thus, near-haploid ALL may represent a unique subgroup of ALL with a poor prognosis. To detect these and other possible subgroups, we have included cytogenetic analysis and flow cytometric analysis of DNA content in our initial evaluation of patients with ALL.

scholarly journals Near-haploid acute lymphoblastic leukemia: a unique subgroup with a poor prognosis?

Blood ◽  
1981 ◽  
Vol 58 (1) ◽  
pp. 14-19 ◽  
Author(s):  
GM Brodeur ◽  
DL Williams ◽  
AT Look ◽  
WP Bowman ◽  
DK Kalwinsky
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Dna Content ◽  
Poor Prognosis ◽  
Lymphoblastic Leukemia ◽  
Periodic Acid ◽  
Flow Cytometric Analysis ◽  
Chromosome 21 ◽  
Periodic Acid Schiff ◽  
Flow Cytometric ◽  
Block Pattern

Abstract We describe two adolescent girls with acute lymphoblastic leukemia (ALL) whose leukemia cells were near-haploid. Their lymphoblasts stained in a block pattern with periodic acid Schiff and had “common ALL” surface markers confirmed by indirect immunofluorescence. Each patient had two populations of blasts, one near-haploid and one hyperdiploid, which was an exact doubling of the near-haploid karyotype. The first patient had a predominant population of cells with 26 chromosomes and a few with 52, while the second had a predominance of cells with 56 and a minority with 28. Flow cytometric analysis of DNA content initially detected the minor near-haploid population in the second patient, which was confirmed later by cytogenetic review of the marrow sample. In addition to our two patients, only four patients have been reported with near-haploid ALL. Of these six, five were girls, five were adolescents, and five had short survivals (median, 10 mo). All six had disomy of chromosome 21 with or without disomy for chromosomes 10, 14, 18, or X (four patients each). Thus, near-haploid ALL may represent a unique subgroup of ALL with a poor prognosis. To detect these and other possible subgroups, we have included cytogenetic analysis and flow cytometric analysis of DNA content in our initial evaluation of patients with ALL.

scholarly journals STRUCTURE OF SOME STRIGEATAS' ORGAN OF BRANDES

2019 ◽  
pp. 399-403
Author(s):  
Nacheva ◽  
Manikovskaya
Keyword(s):  
Periodic Acid ◽  
Secretory Activity ◽  
Bromphenol Blue ◽  
Main Function ◽  
Periodic Acid Schiff ◽  
Alaria Alata ◽  
Sudan Black B ◽  
Glandular Cells ◽  
Secondary Function ◽  
Schiff Reaction

The purpose of the research: the analysis of histology and histochemistry of the Brandes organ of Strigea strigis and Alaria alata. The trematode marites of Strigea striges and Alaria alata were the material. Fixation of the material was carried out in 10% neutral formalin. The treatment of the specimens was carried out using the conventional histological and histochemical methods: with hematoxylin-eosin according to the method of Van Gizon, Mallory, with Sudan black B, sulema-Bromphenol blue according to Bonhage, with periodic acid Schiff reaction by Mac-Manus, alcian blue according to Stedman and Mowry and with toluidine blue. Histochemical reactions were performed with appropriate controls. The studies have shown that the structure of the Brandes organ of Strigea strigis and Alaria alata differs by their constituents and morphology of glandular cells. The histochemical reactions are similar. The cells of the glandular complex show bromophenolophilia, toluudinophilia and fuchsinophilia in periodic acid Schiff reaction speaks about the glycoprotein nature of the secreted substances. Bromphenophilia and sudanophilia of glandular cells cytoplasm indicate the presence of lipoprotein substances in them. The Brandes of S. strigis and A. alata is a morphofunctional unit, to which the principle of multi-functionality is inherent. It performs the main function – digestion of food components by means of developed glandular structures and specialized secretory activity. Its ability to fix the helminth tightly in the endostatin can be considered a secondary function of the organ.

scholarly journals The Cytochemistry of Acute Leukemia: Observations on Glycogen and Neutral Fat in Bone Marrow Aspirates

Blood ◽  
1969 ◽  
Vol 33 (2) ◽  
pp. 341-347 ◽  
Author(s):  
JOHN M. BENNETT ◽  
THOMAS F. DUTCHER
Keyword(s):  
Bone Marrow ◽  
Acute Leukemia ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Cytochemical Study ◽  
Blast Cells ◽  
Schiff Reaction ◽  
Granulocytic Leukemia ◽  
Oil Red O

Abstract A cytochemical study of 54 cases of acute leukemia utilizing the periodic acid-Schiff reaction for glycogen and Oil Red O for neutral fat was performed on air dried bone marrow smears. The majority of leukemic lymphoblasts revealed significant amounts of glycogen and neutral fat. "Blast" cells in granulocytic leukemia were devoid of glycogen and of neutral fat. The recommendation is made that both of these histochemical stains be employed to attempt to resolve the problem of specific cellular identification in acute leukemia.

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