scholarly journals Effects of insulin and insulin-like growth factor I on growth of human leukemia cells in serum-free and protein-free medium

Blood ◽  
1988 ◽  
Vol 72 (1) ◽  
pp. 66-72
Author(s):  
J Sinclair ◽  
D McClain ◽  
R Taetle
Keyword(s):  
Growth Factor ◽  
Culture Conditions ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
Growth Responses ◽  
Free Medium ◽  
Serum Free ◽  
Human Leukemia Cells ◽  
Igf I ◽  
Protein Free Medium

Human myeloid leukemia cells (HL60) and malignant lymphocytes (Namalwa) were grown in protein-free, Fe-supplemented media and used to study growth responses to insulin and insulin-like growth factor 1 (IGF-I). HL60 cells previously grown in serum-free medium containing microgram quantities of insulin showed an 18-fold reduction in cumulative cell production when grown without insulin. However, the same cells showed reduced or absent growth stimulation with 1 to 100 ng/mL insulin or IGF- I for at least four days following insulin deprivation, indicating that culture conditions modified insulin and IGF-I responses. When the same cells were grown in Fe-supplemented, protein-free medium (RPMI-Fe), insulin and IGF-I caused dose-dependent stimulation of HL60 cell growth with half-maximal stimulation at nanogram concentrations. Namalwa cells grown in protein-free medium showed no response to either hormone. Radioligand binding showed the presence of insulin and IGF-I receptors on both HL60 and Namalwa cells grown in RPMI-Fe. HL60 cells grown in fetal bovine serum had higher, and cells grown with microgram quantities of insulin dramatically reduced, insulin binding. Competitive binding studies and cultures with anti-IGF-I receptor antibody showed insulin and IGF-I stimulated growth through their respective specific receptors. Both insulin and IGF-I stimulate growth of some cultured human leukemia cells, but the presence of insulin or IGF-I receptors alone does not predict growth responses. Culture conditions affect both cellular responses and ligand binding by these hormones and must be closely controlled to study growth responses.

scholarly journals Effects of insulin and insulin-like growth factor I on growth of human leukemia cells in serum-free and protein-free medium

Blood ◽  
1988 ◽  
Vol 72 (1) ◽  
pp. 66-72 ◽  
Author(s):  
J Sinclair ◽  
D McClain ◽  
R Taetle
Keyword(s):  
Growth Factor ◽  
Culture Conditions ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
Growth Responses ◽  
Free Medium ◽  
Serum Free ◽  
Human Leukemia Cells ◽  
Igf I ◽  
Protein Free Medium

Abstract Human myeloid leukemia cells (HL60) and malignant lymphocytes (Namalwa) were grown in protein-free, Fe-supplemented media and used to study growth responses to insulin and insulin-like growth factor 1 (IGF-I). HL60 cells previously grown in serum-free medium containing microgram quantities of insulin showed an 18-fold reduction in cumulative cell production when grown without insulin. However, the same cells showed reduced or absent growth stimulation with 1 to 100 ng/mL insulin or IGF- I for at least four days following insulin deprivation, indicating that culture conditions modified insulin and IGF-I responses. When the same cells were grown in Fe-supplemented, protein-free medium (RPMI-Fe), insulin and IGF-I caused dose-dependent stimulation of HL60 cell growth with half-maximal stimulation at nanogram concentrations. Namalwa cells grown in protein-free medium showed no response to either hormone. Radioligand binding showed the presence of insulin and IGF-I receptors on both HL60 and Namalwa cells grown in RPMI-Fe. HL60 cells grown in fetal bovine serum had higher, and cells grown with microgram quantities of insulin dramatically reduced, insulin binding. Competitive binding studies and cultures with anti-IGF-I receptor antibody showed insulin and IGF-I stimulated growth through their respective specific receptors. Both insulin and IGF-I stimulate growth of some cultured human leukemia cells, but the presence of insulin or IGF-I receptors alone does not predict growth responses. Culture conditions affect both cellular responses and ligand binding by these hormones and must be closely controlled to study growth responses.

scholarly journals Isolated porcine ovarian follicles as a model for the study of hormone and growth factor action on ovarian secretory activity

1998 ◽  
Vol 159 (2) ◽  
pp. 313-321 ◽  
Author(s):  
AV Sirotkin ◽  
AV Makarevich ◽  
J Kotwica ◽  
PG Marnet ◽  
HB Kwon ◽  
...  
Keyword(s):  
Growth Factor ◽  
Ovarian Follicles ◽  
Serum Free Medium ◽  
Free Medium ◽  
Ovarian Steroid ◽  
Serum Free ◽  
Factor Binding ◽  
Estradiol Secretion ◽  
Igf I ◽  
Igfbp 3

The aim of our in vitro experiments with isolated porcine ovarian follicles was to study the effects of gonadotropins, GH, IGF-I and oxytocin (OT) on release of ovarian steroid, OT, IGF-I, insulin-like growth factor-binding protein-3 (IGFBP-3), prostaglandin F (PGF), prostaglandin E (PGE) and cAMP. It was found that quarters of ovarian follicles cultured for 8 days produced significant amounts of progesterone, estradiol-17 beta, OT and IGFBP-3 with peaks of accumulation from the 3rd to the 8th day of culture. Addition of serum promoted progesterone, estradiol and OT release, whilst accumulation of IGFBP-3 was maintained to a greater extent in serum-free medium. GH (10 ng/ml or above) was able to inhibit androstenedione, OT, PGF and IGFBP-3, to stimulate IGF-I and cAMP, and to alter testosterone and PGE release by follicles cultured in serum-supplemented and/or serum-free medium. IGF-I (10 ng/ml or more) inhibited androstenedione and PGF secretion, stimulated testosterone, estradiol, OT and cAMP production, but did not influence progesterone, IGFBP-3 or PGE output in these conditions. OT (100 ng/ml) was able to inhibit androstenedione and to stimulate testosterone, IGF-I, PGF and PGE, but not estradiol or IGFBP-3 release. A stimulatory effect of LH on progesterone and OT and an inhibitory influence of LH on estradiol secretion in the serum-supplemented medium were observed. FSH in these conditions stimulated OT, but not progesterone or estradiol secretion. The use of this experimental model suggests the involvement of gonadotropins, OT, GH and IGF-I in the control of ovarian steroid and nonapeptide hormone, growth factor, growth factor-binding protein, prostaglandin and cyclic nucleotide production. The stimulatory effect of GH on IGF-I, and the stimulatory influence of IGF-I on OT, as well as coincidence of the majority of effects of IGF-I and OT, suggest the existence of a GH-IGF-I-OT axis. On the other hand, the different patterns of action of GH and IGF-I on OT, estrogen and IGFBP-3 suggest that part of the GH effect on ovarian cells is IGF-I independent.

Heptocyte Growth Factor-Pleiotropic Cytokine Produced by Human Leukemia cells

1995 ◽  
Vol 19 (3-4) ◽  
pp. 197-205 ◽  
Author(s):  
Eiichi Godha ◽  
Shuji Nakamura ◽  
Itaru Yamamoto ◽  
Jun Minowada
Keyword(s):  
Growth Factor ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
Human Leukemia Cells

scholarly journals Human leukemia cells synthesize and secrete proteins related to platelet-derived growth factor.

1986 ◽  
Vol 83 (15) ◽  
pp. 5526-5530 ◽  
Author(s):  
P. Pantazis ◽  
L. Lanfrancone ◽  
P. G. Pelicci ◽  
R. Dalla-Favera ◽  
H. N. Antoniades
Keyword(s):  
Growth Factor ◽  
Platelet Derived Growth Factor ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
Human Leukemia Cells

Induction of hepatocyte growth factor/scatter factor by interferon-γ in human leukemia cells

1998 ◽  
Vol 174 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Eiichi Gohda ◽  
Takahiro Takebe ◽  
Tomohiro Sotani ◽  
Shuji Nakamura ◽  
Jun Minowada ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Interferon Γ ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
Scatter Factor ◽  
Human Leukemia Cells ◽  
Hepatocyte Growth

Insulin-like growth factor-I and its autocrine role in growth of MCF-7 human breast cancer cells in culture

1989 ◽  
Vol 3 (3) ◽  
pp. 183-190 ◽  
Author(s):  
K. A. Freed ◽  
A. C. Herington
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Growth Factor ◽  
Breast Cancer Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Factor I ◽  
Igf I ◽  
Mcf 7

ABSTRACT Human MCF-7 breast cancer cells have been studied to determine their suitability as an autocrine model for the synthesis, secretion and action of insulin-like growth factor-I (IGF-I). Secretion of immunoreactive (ir-) IGF-I into serum-free medium was very low (<500 pg/106 cells per day). Northern blot hybridization detected at least two IGF-I messenger RNA transcripts (∼4·6 and ∼1·8 kb) which were similar in size to those reported in other human and rat tissues. IGF-II mRNA was also detected but at low abundance. Cell proliferation was stimulated in a dose-responsive manner by exogenous IGF-I (10–30 ng/ml). Addition of a monoclonal antibody against IGF-I to MCF-7 cells in serum-free medium caused an inhibition of cell proliferation, suggesting that endogenous locally produced IGF-I does play an autocrine/paracrine role in MCF-7 cell growth. Proliferation of MCF-7 cells was sensitive to oestradiol (10 nm) in the absence but not in the presence of the weakly oestrogenic pH indicator phenol red. Neither IGF-I secretion nor IGF-I mRNA synthesis, however, was affected by addition of oestradiol. Similarly, GH, dexamethasone or dexamethasone plus oestradiol had no effect on either parameter. These data indicate that MCF-7 cells synthesize, secrete and respond to IGF-I. The very low levels of ir-IGF-I produced and their apparent lack of hormonal modulation suggest, however, that further studies are required to establish whether IGF-I plays a major physiological role in growth and development of MCF-7 cells.

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