scholarly journals Apoptotic cell death of primed CD45RO+ T lymphocytes in Epstein-Barr virus-induced infectious mononucleosis

Blood ◽  
1992 ◽  
Vol 80 (2) ◽  
pp. 452-458 ◽  
Author(s):  
T Uehara ◽  
T Miyawaki ◽  
K Ohta ◽  
Y Tamaru ◽  
T Yokoi ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Central Feature ◽  
Activated T Cells ◽  
Barr Virus ◽  
Epstein Barr

Abstract The expansion of activated T cells, characterized by the expression of CD45RO as well as HLA-DR antigens, is a central feature in acute infectious mononucleosis (IM) induced by primary infection of Epstein- Barr virus (EBV). However, the fate of these activated T cells in this disease is not clearly understood. We found that, on simple culture, a large proportion of T cells isolated from acute IM patients died rapidly, but only a few T cells from normal individuals did. Morphologic observations and DNA fragmentation analysis showed that the loss of viability of IM T cells after incubation was mediated by apoptosis. IM T cells undergoing apoptosis resided exclusively in the CD45RO+ populations of both CD4+ and CD8+ T cells, most of which were shown to coexpress apoptosis-related Fas antigen. Some cytokines such as interleukin-2 (IL-2), IL-5, and IL-6 could rescue IM T cells from apoptotic cell death. The results seemed to imply that most of primed (CD45RO+) T cells in acute IM might be subject to apoptotic cell death, possibly when leaving from the local sites actively producing certain soluble factors required for their survival. Our studies suggest the programmed cell death of peripheral mature T cells as a mechanism of antigen-driven selection.

scholarly journals Apoptotic cell death of primed CD45RO+ T lymphocytes in Epstein-Barr virus-induced infectious mononucleosis

Blood ◽  
1992 ◽  
Vol 80 (2) ◽  
pp. 452-458 ◽  
Author(s):  
T Uehara ◽  
T Miyawaki ◽  
K Ohta ◽  
Y Tamaru ◽  
T Yokoi ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Central Feature ◽  
Activated T Cells ◽  
Barr Virus ◽  
Epstein Barr

The expansion of activated T cells, characterized by the expression of CD45RO as well as HLA-DR antigens, is a central feature in acute infectious mononucleosis (IM) induced by primary infection of Epstein- Barr virus (EBV). However, the fate of these activated T cells in this disease is not clearly understood. We found that, on simple culture, a large proportion of T cells isolated from acute IM patients died rapidly, but only a few T cells from normal individuals did. Morphologic observations and DNA fragmentation analysis showed that the loss of viability of IM T cells after incubation was mediated by apoptosis. IM T cells undergoing apoptosis resided exclusively in the CD45RO+ populations of both CD4+ and CD8+ T cells, most of which were shown to coexpress apoptosis-related Fas antigen. Some cytokines such as interleukin-2 (IL-2), IL-5, and IL-6 could rescue IM T cells from apoptotic cell death. The results seemed to imply that most of primed (CD45RO+) T cells in acute IM might be subject to apoptotic cell death, possibly when leaving from the local sites actively producing certain soluble factors required for their survival. Our studies suggest the programmed cell death of peripheral mature T cells as a mechanism of antigen-driven selection.

Functional Significance of Adhesion Molecules in Fas-Dependent Apoptotic Cell Death Induced by Interleukin-2-Activated T Cells

1998 ◽  
Vol 27 (4-5) ◽  
pp. 309-322 ◽  
Author(s):  
Yan-Wen Zhou ◽  
Yoshihiro Komada ◽  
Hiroto Inaba ◽  
Takao Deguchi ◽  
Kenji Sugiyama ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Adhesion Molecules ◽  
Functional Significance ◽  
Apoptotic Cell ◽  
Interleukin 2 ◽  
Apoptotic Cell Death ◽  
Activated T Cells

scholarly journals Recruitment during Infectious Mononucleosis of CD3+CD4+CD8+Virus-Specific Cytotoxic T Cells Which Recognise Epstein–Barr Virus Lytic Antigen BHRF1

Virology ◽  
1996 ◽  
Vol 219 (2) ◽  
pp. 489-492 ◽  
Author(s):  
CATHERINE A. WHITE ◽  
SIMONE M. CROSS ◽  
MICHAEL G. KURILLA ◽  
BEVERLEY M. KERR ◽  
CHRISTOPHER SCHMIDT ◽  
...  
Keyword(s):  
T Cells ◽  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Cytotoxic T Cells ◽  
Barr Virus ◽  
Epstein Barr

Activation of Suppressor T Cells during Epstein-Barr-Virus-Induced Infectious Mononucleosis

1979 ◽  
Vol 301 (21) ◽  
pp. 1133-1137 ◽  
Author(s):  
Grovanna Tosato ◽  
Ian Magrath ◽  
Irma Koski ◽  
Nancy Dooley ◽  
Michael Blaese
Keyword(s):  
T Cells ◽  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Suppressor T Cells ◽  
Barr Virus ◽  
Epstein Barr

Kinetics of Epstein-Barr virus load and virus-specific CD8+ T cells in acute infectious mononucleosis

2011 ◽  
Vol 50 (3) ◽  
pp. 244-246 ◽  
Author(s):  
Yo Hoshino ◽  
Kazuo Nishikawa ◽  
Yoshinori Ito ◽  
Kiyotaka Kuzushima ◽  
Hiroshi Kimura
Keyword(s):  
T Cells ◽  
Infectious Mononucleosis ◽  
Cd8 T Cells ◽  
Epstein Barr Virus ◽  
Virus Load ◽  
Barr Virus ◽  
Epstein Barr ◽  
Acute Infectious Mononucleosis ◽  
Kinetics Of

scholarly journals Absence of bcl-2 expression by activated CD45RO+ T lymphocytes in acute infectious mononucleosis supporting their susceptibility to programmed cell death

Blood ◽  
1993 ◽  
Vol 82 (2) ◽  
pp. 521-527 ◽  
Author(s):  
Y Tamaru ◽  
T Miyawaki ◽  
K Iwai ◽  
T Tsuji ◽  
R Nibu ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Infectious Mononucleosis ◽  
Cd8 T Cells ◽  
Apoptotic Cell ◽  
Flow Cytometric Analysis ◽  
Inner Mitochondrial Membrane ◽  
Activated T Cells ◽  
Acute Infectious Mononucleosis

Abstract bcl-2 proto-oncogene encodes an inner mitochondrial membrane protein that blocks programmed cell death (apoptosis). There is now increasing evidence that regulation of bcl-2 expression is a determinant of life or death in normal lymphocytes. We have recently described that activated (CD45RO+) CD4+ and CD8+ T cells in acute infectious mononucleosis (IM) undergo apoptotic cell death on culturing, indicating an activation-driven cell death of mature T cells. In this work, we examine bcl-2 expression by activated T cells in acute IM using a flow-cytometric analysis with an anti-bcl-2 monoclonal antibody (MoAb). It was consistently observed that most T cells from acute IM patients displayed only much less bcl-2, while normal T cells expressed bcl-2 relatively strongly. Multicolor analysis showed that bcl-2- lacking T cells in acute IM were restricted to the CD45RO+ (activated) populations of CD4+, as well as CD8+ T cells. In contrast, the relatively intense levels of bcl-2 were expressed in both CD45RO+ and CD45RO- T-cell populations from normal subjects. This marked difference in bcl-2 expression of CD45RO+ T cells between acute IM and normal controls was also confirmed by Western blot analysis. Activated (CD45RO+) T cells with low bcl-2 expression, but not bcl-2-expressing CD45RO- T cells, in acute IM patients were found to die easily when cultured without added growth factors. However, in normal individuals, both CD45RO+ and CD45RO- T cells were relatively stable on culturing. These findings suggest that lack of bcl-2 expression by activated (CD45RO+) T cells in acute IM might be associated with their susceptibility to programmed cell death.

scholarly journals Absence of bcl-2 expression by activated CD45RO+ T lymphocytes in acute infectious mononucleosis supporting their susceptibility to programmed cell death

Blood ◽  
1993 ◽  
Vol 82 (2) ◽  
pp. 521-527
Author(s):  
Y Tamaru ◽  
T Miyawaki ◽  
K Iwai ◽  
T Tsuji ◽  
R Nibu ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Infectious Mononucleosis ◽  
Cd8 T Cells ◽  
Apoptotic Cell ◽  
Flow Cytometric Analysis ◽  
Inner Mitochondrial Membrane ◽  
Activated T Cells ◽  
Acute Infectious Mononucleosis

bcl-2 proto-oncogene encodes an inner mitochondrial membrane protein that blocks programmed cell death (apoptosis). There is now increasing evidence that regulation of bcl-2 expression is a determinant of life or death in normal lymphocytes. We have recently described that activated (CD45RO+) CD4+ and CD8+ T cells in acute infectious mononucleosis (IM) undergo apoptotic cell death on culturing, indicating an activation-driven cell death of mature T cells. In this work, we examine bcl-2 expression by activated T cells in acute IM using a flow-cytometric analysis with an anti-bcl-2 monoclonal antibody (MoAb). It was consistently observed that most T cells from acute IM patients displayed only much less bcl-2, while normal T cells expressed bcl-2 relatively strongly. Multicolor analysis showed that bcl-2- lacking T cells in acute IM were restricted to the CD45RO+ (activated) populations of CD4+, as well as CD8+ T cells. In contrast, the relatively intense levels of bcl-2 were expressed in both CD45RO+ and CD45RO- T-cell populations from normal subjects. This marked difference in bcl-2 expression of CD45RO+ T cells between acute IM and normal controls was also confirmed by Western blot analysis. Activated (CD45RO+) T cells with low bcl-2 expression, but not bcl-2-expressing CD45RO- T cells, in acute IM patients were found to die easily when cultured without added growth factors. However, in normal individuals, both CD45RO+ and CD45RO- T cells were relatively stable on culturing. These findings suggest that lack of bcl-2 expression by activated (CD45RO+) T cells in acute IM might be associated with their susceptibility to programmed cell death.

Fas/APO‐1 (CD95)‐mediated cytotoxicity is responsible for the apoptotic cell death of leukaemic cells induced by interleukin‐2‐activated T cells

1997 ◽  
Vol 96 (1) ◽  
pp. 147-157 ◽  
Author(s):  
Yoshihiro Komada ◽  
Yan‐Wen Zhou ◽  
Xao‐Li Zhang ◽  
Tong‐Xin Chen ◽  
Shigeki Tanaka ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Interleukin 2 ◽  
Apoptotic Cell Death ◽  
Activated T Cells ◽  
Leukaemic Cells
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