CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells

Abstract CD40 ligand (CD40L)/CD40 interactions play a central role in T-cell–dependent B-cell activation as previously shown by in vitro studies, the phenotype of CD40L knockout mice and the defective expression of CD40L in patients who have X-linked immunodeficiency with hyper-IgM. The distribution of CD40 in cells other than of myeloid and lymphoid lineages has suggested additional functions for this receptor/ligand couple. Here we show that CD40L stimulates myelopoiesis with a noticeable effect on megakaryocytopoiesis in cocultures of hematopoietic progenitor cells and bone marrow stromal cells. These results suggest a mechanism by which T-cell or platelet-associated or soluble CD40L may regulate myelopoiesis.

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CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells

Blood ◽

10.1182/blood.v95.12.3758.012k44_3758_3764 ◽

2000 ◽

Vol 95 (12) ◽

pp. 3758-3764 ◽

Cited By ~ 3

Author(s):

Anne Solanilla ◽

Julie Déchanet ◽

Abdel El Andaloussi ◽

Moryse Dupouy ◽

François Godard ◽

...

Keyword(s):

Bone Marrow ◽

T Cell ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Cell Activation ◽

Cd40 Ligand ◽

Marrow Stromal Cells ◽

B Cell Activation ◽

Noticeable Effect

CD40 ligand (CD40L)/CD40 interactions play a central role in T-cell–dependent B-cell activation as previously shown by in vitro studies, the phenotype of CD40L knockout mice and the defective expression of CD40L in patients who have X-linked immunodeficiency with hyper-IgM. The distribution of CD40 in cells other than of myeloid and lymphoid lineages has suggested additional functions for this receptor/ligand couple. Here we show that CD40L stimulates myelopoiesis with a noticeable effect on megakaryocytopoiesis in cocultures of hematopoietic progenitor cells and bone marrow stromal cells. These results suggest a mechanism by which T-cell or platelet-associated or soluble CD40L may regulate myelopoiesis.

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A Method for Measurement of Drug Sensitivity of Myeloma Cells Co-Cultured with Bone Marrow Stromal Cells

CrossRef Listing of Deleted DOIs ◽

10.1177/1087057113478168 ◽

2013 ◽

Vol 18 (6) ◽

pp. 637-646 ◽

Cited By ~ 18

Author(s):

Kristine Misund ◽

Katarzyna A. Baranowska ◽

Toril Holien ◽

Christoph Rampa ◽

Dionne C. G. Klein ◽

...

Keyword(s):

Bone Marrow ◽

Cell Survival ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Large Scale ◽

Stromal Cell ◽

Marrow Stromal Cells ◽

Cell Nuclei ◽

Myeloma Cells

The tumor microenvironment can profoundly affect tumor cell survival as well as alter antitumor drug activity. However, conventional anticancer drug screening typically is performed in the absence of stromal cells. Here, we analyzed survival of myeloma cells co-cultured with bone marrow stromal cells (BMSC) using an automated fluorescence microscope platform, ScanR. By staining the cell nuclei with DRAQ5, we could distinguish between BMSC and myeloma cells, based on their staining intensity and nuclear shape. Using the apoptotic marker YO-PRO-1, the effects of drug treatment on the viability of the myeloma cells in the presence of stromal cells could be measured. The method does not require cell staining before incubation with drugs, and less than 5000 cells are required per condition. The method can be used for large-scale screening of anticancer drugs on primary myeloma cells. This study shows the importance of stromal cell support for primary myeloma cell survival in vitro, as half of the cell samples had a marked increase in their viability when cultured in the presence of BMSC. Stromal cell–induced protection against common myeloma drugs is also observed with this method.

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In vitro differentiation of human bone marrow stromal cells into neural precursor cells using small molecules

Journal of Neuroscience Methods ◽

10.1016/j.jneumeth.2021.109340 ◽

2021 ◽

Vol 363 ◽

pp. 109340

Author(s):

Abeer Sallam ◽

Thangirala Sudha ◽

Noureldien H.E. Darwish ◽

Samar Eghotny ◽

Abeer E-Dief ◽

...

Keyword(s):

Bone Marrow ◽

Small Molecules ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Precursor Cells ◽

Marrow Stromal Cells ◽

Neural Precursor Cells ◽

Neural Precursor ◽

In Vitro Differentiation

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FGF7 supports hematopoietic stem and progenitor cells and niche-dependent myeloblastoma cells via autocrine action on bone marrow stromal cells in vitro

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2013.09.044 ◽

2013 ◽

Vol 440 (1) ◽

pp. 125-131 ◽

Cited By ~ 9

Author(s):

Ruri Ishino ◽

Kaori Minami ◽

Satowa Tanaka ◽

Mami Nagai ◽

Keiji Matsui ◽

...

Keyword(s):

Bone Marrow ◽

Progenitor Cells ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Marrow Stromal Cells ◽

Hematopoietic Stem ◽

Stem And Progenitor Cells

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Danggui Buxue Tang promotes the adhesion and migration of bone marrow stromal cells via the focal adhesion pathway in vitro

Journal of Ethnopharmacology ◽

10.1016/j.jep.2018.11.018 ◽

2019 ◽

Vol 231 ◽

pp. 90-97 ◽

Cited By ~ 4

Author(s):

Huaben Bo ◽

Junhua He ◽

Xiao Wang ◽

Ruilan Du ◽

Haikang Bei ◽

...

Keyword(s):

Bone Marrow ◽

Focal Adhesion ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Marrow Stromal Cells ◽

Danggui Buxue Tang ◽

And Migration ◽

Focal Adhesion Pathway

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Construction of Tissue Engineering Bone with Osteoactivin Gene Transfected Rabbit Bone Marrow Stromal Cells and Porous Silk Fibroin Scaffold in Vitro

Advances in Clinical Medicine ◽

10.12677/acm.2014.42006 ◽

2014 ◽

Vol 04 (02) ◽

pp. 27-33

Author(s):

涵王

Keyword(s):

Tissue Engineering ◽

Bone Marrow ◽

Silk Fibroin ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Marrow Stromal Cells ◽

Silk Fibroin Scaffold ◽

Rabbit Bone

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Combination of hTERT and bmi-1, E6, or E7 Induces Prolongation of the Life Span of Bone Marrow Stromal Cells from an Elderly Donor without Affecting Their Neurogenic Potential

Molecular and Cellular Biology ◽

10.1128/mcb.25.12.5183-5195.2005 ◽

2005 ◽

Vol 25 (12) ◽

pp. 5183-5195 ◽

Cited By ~ 120

Author(s):

Taisuke Mori ◽

Tohru Kiyono ◽

Hideaki Imabayashi ◽

Yukiji Takeda ◽

Kohei Tsuchiya ◽

...

Keyword(s):

Bone Marrow ◽

Life Span ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Marrow Stromal Cells ◽

Murine Bone Marrow ◽

Differentiated Cells ◽

E6 And E7 ◽

Old Donor

ABSTRACT Murine bone marrow stromal cells differentiate not only into mesodermal derivatives, such as osteocytes, chondrocytes, adipocytes, skeletal myocytes, and cardiomyocytes, but also into neuroectodermal cells in vitro. Human bone marrow stromal cells are easy to isolate but difficult to study because of their limited life span. To overcome this problem, we attempted to prolong the life span of bone marrow stromal cells and investigated whether bone marrow stromal cells modified with bmi-1, hTERT, E6, and E7 retained their differentiated capability, or multipotency. In this study, we demonstrated that the life span of bone marrow stromal cells derived from a 91-year-old donor could be extended and that the stromal cells with an extended life span differentiated into neuronal cells in vitro. We examined the neuronally differentiated cells morphologically, physiologically, and biologically and compared the gene profiles of undifferentiated and differentiated cells. The neuronally differentiated cells exhibited characteristics similar to those of midbrain neuronal progenitors. Thus, the results of this study support the possible use of autologous-cell graft systems to treat central nervous system diseases in geriatric patients.

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