scholarly journals Immunogenicity of recombinant Mycobacterium bovis bacille Calmette–Guèrin clones expressing T and B cell epitopes of Mycobacterium tuberculosis antigens

2013 ◽  
Vol 14 (Suppl 1) ◽  
pp. S5 ◽  
Author(s):  
Rohimah Mohamud ◽  
Maryam Azlan ◽  
Daniel Yero ◽  
Nadine Alvarez ◽  
Maria E Sarmiento ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
B Cell ◽  
Mycobacterium Bovis ◽  
Bacille Calmette Guerin ◽  
B Cell Epitopes

scholarly journals Nicotinamide Limits Replication of Mycobacterium tuberculosis and Bacille Calmette-Guérin Within Macrophages

2019 ◽  
Vol 221 (6) ◽  
pp. 989-999
Author(s):  
Jason D Simmons ◽  
Glenna J Peterson ◽  
Monica Campo ◽  
Jenny Lohmiller ◽  
Shawn J Skerrett ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nicotinic Acid ◽  
Mycobacterium Bovis ◽  
Related Compound ◽  
Antimicrobial Properties ◽  
Broth Culture ◽  
Wild Type ◽  
Bacille Calmette Guerin ◽  
Macrophage Function ◽  
Modest Activity

Abstract Novel antimicrobials for treatment of Mycobacterium tuberculosis are needed. We hypothesized that nicotinamide (NAM) and nicotinic acid (NA) modulate macrophage function to restrict M. tuberculosis replication in addition to their direct antimicrobial properties. Both compounds had modest activity in 7H9 broth, but only NAM inhibited replication in macrophages. Surprisingly, in macrophages NAM and the related compound pyrazinamide restricted growth of bacille Calmette-Guérin but not wild-type Mycobacterium bovis, which both lack a functional nicotinamidase/pyrazinamidase (PncA) rendering each strain resistant to these drugs in broth culture. Interestingly, NAM was not active in macrophages infected with a virulent M. tuberculosis mutant encoding a deletion in pncA. We conclude that the differential activity of NAM and nicotinic acid on infected macrophages suggests host-specific NAM targets rather than PncA-dependent direct antimicrobial properties. These activities are sufficient to restrict attenuated BCG, but not virulent wild-type M. bovis or M. tuberculosis.

scholarly journals Priming by DNA Immunization Augments Protective Efficacy of Mycobacterium bovis Bacille Calmette-Guerin against Tuberculosis

2001 ◽  
Vol 69 (6) ◽  
pp. 4174-4176 ◽  
Author(s):  
Carl G. Feng ◽  
Umaimainthan Palendira ◽  
Caroline Demangel ◽  
Joanne M. Spratt ◽  
Adam S. Malin ◽  
...  
Keyword(s):  
T Cells ◽  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Protective Efficacy ◽  
Mycobacterial Antigen ◽  
Dna Immunization ◽  
Bacille Calmette Guerin

ABSTRACT Sequential immunization with mycobacterial antigen Ag85B-expressing DNA and Mycobacterium bovis bacille Calmette-Guerin (BCG) was more effective than BCG immunization in protecting againstMycobacterium tuberculosis infection. Depletion of the CD8+ T cells in the immunized mice impaired protection in their spleens, indicating that this improved efficacy was partially mediated by CD8+ T cells.

scholarly journals Comparison of the Protective Efficacy of Bacille Calmette-Guérin Vaccination against Aerosol Challenge with Mycobacterium tuberculosis and Mycobacterium bovis

2000 ◽  
Vol 30 (Supplement_3) ◽  
pp. S299-S301 ◽  
Author(s):  
Ann Williams ◽  
Angela Davies ◽  
Philip D. Marsh ◽  
Mark A. Chambers ◽  
R. Glyn Hewinson
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Protective Efficacy ◽  
Bacille Calmette Guerin

scholarly journals A Human Challenge Model for Mycobacterium tuberculosis Using Mycobacterium bovis Bacille Calmette-Guérin

2012 ◽  
Vol 205 (7) ◽  
pp. 1035-1042 ◽  
Author(s):  
Angela M. Minassian ◽  
Iman Satti ◽  
Ian D. Poulton ◽  
Joel Meyer ◽  
Adrian V. S. Hill ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Bacille Calmette Guerin

scholarly journals T- and B-Cell Epitopes in the Secreted Mycobacterium bovis Antigen MPB70 in Mice

2003 ◽  
Vol 57 (2) ◽  
pp. 151-161 ◽  
Author(s):  
S. Tollefsen ◽  
J. M. Pollock ◽  
T. Lea ◽  
M. Harboe ◽  
H. G. Wiker
Keyword(s):  
B Cell ◽  
Mycobacterium Bovis ◽  
B Cell Epitopes

scholarly journals Prediction of B-cell epitope by in silico analysis of Mycobacterium tuberculosis Ag85B antigen

2020 ◽  
pp. 101-109
Author(s):  
Nihayatul Karimah ◽  
Sabar Pambudi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
B Cell ◽  
In Silico ◽  
Cell Epitope ◽  
Docking Simulation ◽  
In Silico Analysis ◽  
Protein Biomarkers ◽  
B Cell Epitopes ◽  
Good Target ◽  
Silico Analysis

Mycobacterium tuberculosis (Mtb) is a causative pathogen of tuberculosis (TB) that emerges as one of the deadliest communicable diseases in Indonesia. The quest for protein biomarkers for TB has been conducted in order to develop a TB diagnostic kit and a TB vaccine. One of the abundant biomarkers in the TB infected human serum is the Ag85B antigen. In this study, we employed immunoinformatic prediction tools such as Ellipro and VaxiJen to predict the B-cell epitopes of Ag85B wildtype and multidrug resistance type (mutant). We then performed molecular docking simulation to evaluate the predicted epitopes using HADDOCK. The screening of both continuous and discontinuous B-cell epitopes using criteria-based analysis resulted in the eight linear epitopes and two conformational epitopes in Ag85B with high antigenicity. The in silico analysis showed no major differences between Ag85B wildtype and Ag85B mutant, implying Ag85B a good target for TB vaccine candidates but not for a specific biomarker that differentiates wild-type and mutant TB.

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