Lymphocyte and monocyte flow cytometry immunophenotyping as a diagnostic tool in uncharacteristic inflammatory disorders

Cytological examination is a valuable diagnostic tool in case of a serous effusion. The firstmanifestation of malignancy may be an effusion of the pleural, pericardial, or peritoneal cavity, especially in carcinoma of the ovary, or lung, and malignant mesothelioma. In other malignancies effusions may occur in the course of the disease. The contribution by Motherby et al. in this issue of ACP focuses on the contribution of image and flow cytometry to establish the presence or absence of malignancy in serous effusions [16]. They point out that the sensitivity of DNA image cytometry in equivocal effusions may be as high as 87.5%, and that for the detection of malignancy, DNA image cytometry is superior to flow cytometry.

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Performance evaluation of anti-fixed Leishmania infantum promastigotes immunoglobulin G (IgG) detected by flow cytometry as a diagnostic tool for visceral Leishmaniasis

Journal of Immunological Methods ◽

10.1016/j.jim.2019.02.009 ◽

2019 ◽

Vol 469 ◽

pp. 18-25 ◽

Cited By ~ 2

Author(s):

Elis D. Silva ◽

Beatriz C. Oliveira ◽

Andresa P. Oliveira ◽

Wagner J.T. Santos ◽

George T. Diniz ◽

...

Keyword(s):

Flow Cytometry ◽

Performance Evaluation ◽

Visceral Leishmaniasis ◽

Immunoglobulin G ◽

Diagnostic Tool ◽

Leishmania Infantum

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Impact of Flow Cytometry in Liver Cytopathology

Acta Cytologica ◽

10.1159/000370042 ◽

2015 ◽

Vol 59 (1) ◽

pp. 51-60 ◽

Cited By ~ 1

Author(s):

Jennifer P. Bynum ◽

Amy Duffield ◽

Syed Z. Ali

Keyword(s):

Flow Cytometry ◽

Fine Needle Aspiration ◽

Diagnostic Tool ◽

Needle Aspiration ◽

Lymphoproliferative Disorders ◽

Johns Hopkins Hospital ◽

Lymphoproliferative Diseases ◽

Fine Needle ◽

Negative Results

Background: Fine needle aspiration (FNA) is commonly used as a diagnostic tool for the evaluation of lymphoproliferative diseases. Cytomorphology alone is often insufficient for the diagnosis and subclassification of lymphoma; therefore, flow cytometry (FC) plays an important role in the characterization of lymphoproliferative disorders. This study reviews our experience with FC on liver FNA at the Johns Hopkins Hospital. Methods: 2,424 liver FNAs performed over a 21-year period were reviewed for clinical FC data (n = 74) or a subsequent diagnosis of lymphoma in the liver without FC data (n = 40). Results: In our study, 114 cases (4.7%) were included out of the 2,424 liver FNAs performed during the study period. Lymphoma was diagnosed 79 times. Cytomorphology alone was diagnostic of lymphoma in 45 cases, and in 33 cases both the cytomorphology and the FC were consistent with a diagnosis of lymphoma. Neither FC nor cytomorphology were diagnostic of lymphoma on 1 specimen. In 39 cases, FC had negative results on a lesion suspicious for lymphoma based on cytomorphology. In several nonlymphoma cases, FC provided information that allowed further subclassification of the neoplasm. Conclusion: FC is a useful adjuvant diagnostic test for liver FNAs performed on patients with lymphoproliferative disorders.

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Flow Cytometry as a Diagnostic Tool for Hereditary Spherocytosis

Acta Haematologica ◽

10.1159/000094679 ◽

2006 ◽

Vol 116 (3) ◽

pp. 186-191 ◽

Cited By ~ 22

Author(s):

Gudrun Stoya ◽

Bernd Gruhn ◽

Heinz Vogelsang ◽

Eckehard Baumann ◽

Werner Linss

Keyword(s):

Flow Cytometry ◽

Diagnostic Tool ◽

Hereditary Spherocytosis

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PRELIMINARY RESULTS ON THE USE OF FLOW CYTOMETRY AS A DIAGNOSTIC TOOL FOR THE IDENTIFICATION OF POLYEMBRYONIC CITRUS ROOTSTOCK HYRBIDS

Acta Horticulturae ◽

10.17660/actahortic.2013.1007.85 ◽

2013 ◽

pp. 733-738

Author(s):

K.F. Hannweg ◽

G. Visser ◽

A. Sippel

Keyword(s):

Flow Cytometry ◽

Diagnostic Tool ◽

Preliminary Results ◽

Citrus Rootstock

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F-18 Fluorodeoxyglucose PET/CT as a Diagnostic Tool in Orbital Inflammatory Disorders

10.21203/rs.3.rs-145990/v1 ◽

2021 ◽

Author(s):

Ghassan Elourimi ◽

Michael Soussan ◽

Matthieu Groh ◽

Antoine Martin ◽

Françoise Héran ◽

...

Keyword(s):

Autoimmune Diseases ◽

Diagnostic Tool ◽

Fdg Pet ◽

Final Diagnosis ◽

Diagnostic Workup ◽

Routine Practice ◽

Inflammatory Disorders ◽

Tertiary Referral Center ◽

Pet Ct ◽

Fdg Pet Ct

Abstract Background: In the last decade, FDG-PET/CT has become routine practice in the management of lymphoma or autoimmune diseases. In the current study, we aimed to assess the usefulness of FDG-PET/CT as a potential diagnostic tool for detecting underlying systemic diseases (SD) in patients with orbital inflammatory disorders (OID).Methods: All consecutive patients managed for new-onset OID between 2011 and 2018 in a tertiary referral center for OID, who underwent FDG-PET/CT as part as the etiological diagnostic workup were enrolled. PET-FDG/CT scans were reviewed blindly and were considered as positive for SD detection if they showed lymphadenopathy and/or other visceral lesions with an uptake above blood pool background. We used the standard diagnostic workup (performed in all patients at presentation) as relevant comparator. To quantify the incremental value of FDG-PET/CT over the standard diagnostic workup, the Net Reclassification Index (NRI) and Integrated Discrimination Index (IDI) were used. The final diagnosis was based on international criteria for autoimmune diseases, or histological confirmation for lymphoma, xanthogranuloma, crystal storing histiocytosis (CSH), or idiopathic orbital inflammation syndrome (IOIS). Results: Among the 22 patients enrolled, 14 (63%) had underlying SD (granulomatosis with polyangiitis (GPA), n=1; IgG4-related disease (IgG4-RD), n=5; CSH, n=1; adult onset asthma and periocular xanthogranuloma (AAPOX), n=3; lymphoma, n=4) while the remaining 8 (37%) patients were diagnosed with IOIS. Eleven (50%) patients had a positive FDG-PET/CT. After clinicobiological evaluation, FDG-PET/CT correctly reclassified 29% of patients with SD (p=0.04) and 13% with IOIS (p=0.3), corresponding to an elevated NRI of 0.41±0.17 (p=0.03). The IDI test used to evaluate the improvement of FDG-PET/CT for SD detection was 0.38±0.08 (p<0.001). After FDG-PET/CT, probability changes for SD and IOIS were measured at 0.14 and -0.24, respectively (relative gain of 3.04 for IDI). FDG-PET/CT successfully detected asymptomatic lesions in all patients with a final diagnosis of lymphoma. Conclusion: FDG-PET/CT enabled accurate reclassification of more than one quarter of patients with SD, suggesting its potential value for detecting SD (especially extraorbital lymphoma).

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