Performance evaluation of anti-fixed Leishmania infantum promastigotes immunoglobulin G (IgG) detected by flow cytometry as a diagnostic tool for visceral Leishmaniasis

2019 ◽  
Vol 469 ◽  
pp. 18-25 ◽  
Author(s):  
Elis D. Silva ◽  
Beatriz C. Oliveira ◽  
Andresa P. Oliveira ◽  
Wagner J.T. Santos ◽  
George T. Diniz ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Performance Evaluation ◽  
Visceral Leishmaniasis ◽  
Immunoglobulin G ◽  
Diagnostic Tool ◽  
Leishmania Infantum

scholarly journals Differentiation between canine cutaneous and visceral leishmaniasis by the detection of immunoglobulin G specific for Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi antigens using flow cytometry

2008 ◽  
Vol 154 (3-4) ◽  
pp. 341-346 ◽  
Author(s):  
Marta de Almeida Santiago ◽  
Flávia Coelho Ribeiro ◽  
Eliame Mouta-Confort ◽  
Lílian Dias Nascimento ◽  
Armando de Oliveira Schubach ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Visceral Leishmaniasis ◽  
Immunoglobulin G ◽  
Leishmania Chagasi

scholarly journals Detection of Anti-Leishmania (Leishmania) chagasi Immunoglobulin G by Flow Cytometry for Cure Assessment following Chemotherapeutic Treatment of American Visceral Leishmaniasis

2007 ◽  
Vol 14 (5) ◽  
pp. 569-576 ◽  
Author(s):  
Elenice Moreira Lemos ◽  
Izabelle Teixeira Gomes ◽  
Sílvio Fernando Guimarães Carvalho ◽  
Roberta Dias Rodrigues Rocha ◽  
Jauber Fornaciari Pissinate ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Visceral Leishmaniasis ◽  
Immunoglobulin G ◽  
Leishmania Chagasi ◽  
Performance Indices ◽  
Negative Results ◽  
Serological Reactivity ◽  
New Interpretation ◽  
Low Efficiency ◽  
Chemotherapeutic Treatment

ABSTRACTThe residual serological reactivity observed in patients cured of visceral leishmaniasis (VL) represents the major factor underlying the low efficiency of most anti-Leishmaniaserological approaches to assess posttherapeutic cure in VL. Herein, we have described a detuned flow cytometry-based methodology to detect anti-live (FC-ALPA-immunoglobulin G [IgG]) and anti-fixed (FC-AFPA-IgG)L. chagasipromastigote IgG, along the titration curve (1:2,000 to 1:128,000), as a tool to assess late (12 months after treatment [12 mAT]) and early (2 and 6 mAT) posttherapeutic cure of pediatric American visceral leishmaniasis. Reactivities were reported as the percentage of positive fluorescent parasite (PPFP), using a PPFP of 50% as a cutoff to segregate positive and negative results. Our data demonstrated that both FC-ALPA-IgG at 1:4,000 and FC-ALPA-IgG at 1:32,000 are useful for late cure assessment in VL, with 100% specificity and outstanding likelihood ratio indices. Cure assessment at 6 mAT also showed promising performance indices, identifying 81% and 71.4% of the treated patients with negative results. However, new interpretation parameters were necessary to monitor cure at 2 mAT. We then introduced the differential PPFP (ΔPPFP) of 25% as a new cutoff for early cure assessment at specific serum dilutions to analyze IgG reactivity by FC-ALPA-IgG and FC-AFPA-IgG. Our data demonstrated that at 2 mAT, ΔPPFP was >25% in 60% and 57.1% of treated patients, whereas at 6 mAT, a ΔPPFP of >25% was observed in 100% and 95.2% of samples assayed by FC-ALPA-IgG and FC-AFPA-IgG, respectively. Together, our findings showed the potential of both FC-ALPA-IgG and FC-AFPA-IgG regarding their applicability to detect differential serological reactivity and further contribution to posttherapeutic cure assessment in VL.

scholarly journals A Flow Cytometry-Based Serological Assay to Detect Visceral Leishmaniasis in HIV-Infected Patients

2021 ◽  
Vol 8 ◽  
Author(s):  
Elis D. da Silva ◽  
Beatriz C. de Oliveira ◽  
Allana M. de S. Pereira ◽  
Diego L. Guedes ◽  
Osvaldo P. de Melo Neto ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Clinical Status ◽  
Humoral Response ◽  
Diagnostic Value ◽  
Roc Curve Analysis ◽  
Serological Tests ◽  
Negative Results ◽  
And Performance

Visceral Leishmaniasis (VL) is a severe parasitic disease that has emerged as an important opportunistic condition in HIV-infected patients and whose control is impaired by inaccurate identification. This is mainly due to the serological tests used for VL having a reduced performance in cases of VL-HIV coinfection due to a low humoral response. In this situation, however, a positive test has even greater diagnostic value when combined with the clinical status. This study aimed to evaluate the application and performance of flow cytometry to detect anti-Leishmania infantum antibodies in HIV-infected patients. Sera from VL/HIV coinfected patients, characterized using “gold standard” techniques, were compared with sera from healthy controls plus sera from HIV-infected individuals. The flow cytometry results were expressed as levels of IgG reactivity, based on the percentage of positive fluorescent parasites (PPFP). A ROC curve analysis of a serum titration indicated a PPFP of 1.26% as being the cutoff point to segregate positive and negative results. At the 1:2,048 dilution, with 89% sensitivity and 83% specificity, flow cytometry showed greater sensitivity in relation to the serological tests evaluated. Futhermore, flow cytometry was the only assay that positively identified all VL-HIV patients with quantified HIV load. Together, these findings suggest that flow cytometry may be used as an alternative serological approach for VL identification and as a tool to characterize the humoral response against Leishmania infantum in HIV-infected patients.

scholarly journals Cutaneous Leishmaniasis in Algeria; Highlight on the Focus of M’Sila

2021 ◽  
Vol 9 (5) ◽  
pp. 962
Author(s):  
Razika Beniklef ◽  
Karim Aoun ◽  
Karim Boudrissa ◽  
Meriem Ben Abid ◽  
Kamel Cherif ◽  
...  
Keyword(s):  
Risk Factors ◽  
Visceral Leishmaniasis ◽  
Cutaneous Leishmaniasis ◽  
Leishmania Infantum ◽  
Psammomys Obesus ◽  
Phlebotomus Papatasi ◽  
Wild Rodents ◽  
High Incidence ◽  
Medical Importance ◽  
Underlying Risk

Algeria ranks second after Afghanistan for the incidence of cutaneous leishmaniasis (CL) worldwide. Here, we report a 34-years retrospective analysis of CL in Algeria and focused on the most affected region, the M’Sila province. All 66 cutaneous isolates corresponded to Leishmania (L.) major. Our study of the sandfly and rodent fauna further highlighted the high density of Phlebotomus papatasi and additional phlebotomine species of medical importance, not previously identified in M’Sila. Wild rodents belonging to nine species were trapped in M’Sila, and Psammomys obesus and Meriones shawi were found infected by L. major. In addition, Leishmania infantum was isolated from two visceral leishmaniasis cases, one dog and its proven vectors (P. perniciosus, P. longicuspis, and P. perfiliewi) inventoried during the survey. The high incidence of CL in the M’Sila province is likely a consequence of the increase in minimum temperatures recorded that constitutes suitable conditions for establishing a high endemicity and leads to an explosive rise in leishmaniases cases in this region. A thorough investigation of the underlying risk factors is urgently needed to detect new cases earlier. All these would improve the preparedness to fight the disease.

scholarly journals Experimental Evaluation of Second-Line Oral Treatments of Visceral Leishmaniasis Caused by Leishmania infantum

1999 ◽  
Vol 43 (1) ◽  
pp. 172-174 ◽  
Author(s):  
Jean-Pierre Gangneux ◽  
Michael Dullin ◽  
Annie Sulahian ◽  
Yves Jean-Francois Garin ◽  
Francis Derouin
Keyword(s):  
Visceral Leishmaniasis ◽  
Murine Model ◽  
Experimental Evaluation ◽  
Leishmania Infantum ◽  
Parasite Load ◽  
Second Line ◽  
Meglumine Antimoniate ◽  
Marked Activity

ABSTRACT In a murine model of Leishmania infantum visceral leishmaniasis, metronidazole, ketoconazole, fluconazole, itraconazole, and terbinafine were less effective than antimonial agents in reducing hepatic parasite load. Ketoconazole potentiated the effect of meglumine antimoniate reference therapy through its marked activity against spleen infection.

Flow cytometry as a diagnostic tool in neuroblastoma

2021 ◽  
pp. 113135
Author(s):  
Gislaine Furlanetto ◽  
Fabiane Spagnol ◽  
Ana Paula Alegretti ◽  
Mariela Granero Farias ◽  
Victor Jablonski Soares ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Diagnostic Tool

scholarly journals Sexual and vertical transmission of visceral leishmaniasis

2014 ◽  
Vol 8 (04) ◽  
pp. 403-407 ◽  
Author(s):  
Andreia P Turchetti ◽  
Tayse D Souza ◽  
Tatiane A Paixão ◽  
Renato L. Santos
Keyword(s):  
Visceral Leishmaniasis ◽  
Vertical Transmission ◽  
Leishmania Infantum ◽  
Vertebrate Host ◽  
Domestic Dog ◽  
Sand Flies ◽  
Phlebotomine Sand Flies

Visceral leishmaniasis (VL) is an important zoonosis caused by Leishmania infantum, which has in the domestic dog its principal vertebrate host. VL is usually transmitted by phlebotomine sand flies, however atypical routes of transmission have been described. In this review we discuss the the role of sexual and vertical transmissions, and their role in the maintenance of VL in canine populations.

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