scholarly journals Antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to alternative topical agents at Jimma University Specialized Hospital, South-West Ethiopia

2014 ◽  
Vol 13 (1) ◽  
pp. 14 ◽  
Author(s):  
Mohammedaman Mama ◽  
Alemseged Abdissa ◽  
Tsegaye Sewunet
Keyword(s):  
Wound Infection ◽  
Antimicrobial Susceptibility ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
South West ◽  
Specialized Hospital ◽  
South West Ethiopia ◽  
Jimma University

scholarly journals Bacterial profile, antibiotic susceptibility pattern and associated risk factors of urinary tract infection among clinically suspected children attending at Felege-Hiwot Comprehensive and Specialized Hospital, Northwest Ethiopia. A prospective study

2020 ◽  
Author(s):  
Adugna Fenta ◽  
Mulat Dagnew ◽  
Setegn Eshetie ◽  
Teshome Belachew
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Susceptibility ◽  
Northwest Ethiopia ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Gram Negative ◽  
Antimicrobial Susceptibility Pattern ◽  
Specialized Hospital

Abstract Background: Urinary tract infection is one of the most common bacterial infections in children. Understanding the characteristics of uropathogens and their antimicrobial susceptibility pattern in a particular setting can provide evidence for the appropriate management of cases. This study aimed to assess the bacterial profile of urinary tract infection, their antimicrobial susceptibility pattern and associated factors among clinically suspected children attending at Felege-Hiwot Comprehensive Specialized Hospital, Northwest Ethiopia.Methods: A hospital-based cross-sectional study was conducted from February-April, 2019. A systematic sampling technique was employed. A mid-stream urine sample was inoculated on cystine lactose electrolyte deficient media and incubated for 24-48 hours. Sub-culturing was done on Mac-Conkey and blood agar. Antimicrobial susceptibility test was done on Muller-Hinton agar. A binary logistic regression model was used to see the association between dependent and independent factors. A p-value<0.05 at 95% CI was considered as statistically significant.Results: The overall prevalence of urinary tract infection was 16.7% (95% CI 12.4-21.1). Both Gram-negative and Gram-positive bacterial isolates were recovered with a rate of 44/50 (88%) and 6/50 (12%) respectively. Among Gram-negative isolates, E. coli 28/44(63.6%) was predominant while S. saprophyticus 2/6(33.3%) was prevalent among Gram-positive bacterial isolates. Overall, a high level of resistance to ampicillin, augmentin, and tetracycline was shown by Gram-negative bacteria with a rate of 44/44(100%), 39/44(88.6%), and36/44 (81.8%) respectively. About 33/50(66%) of overall multidrug resistance was observed (95% CI 52-78). About six Gram-negative bacterial isolates were extended spectrum beta-lactamase (ESBL) producers. Having a history of urinary tract infection (P-0.003, AOR 1.86-22.15) and male uncircumcision (p-0.00, AOR 5.5-65.35) were the independent variables that associate for urinary tract infections.Conclusion: In the present study, the prevalence of urinary tract infection among children was high and considerably a high proportion of multidrug resistance was observed. This result will have a significant impact on the selection of appropriate antimicrobial agents for the treatment of urinary tract infection.

scholarly journals Bacterial profile, antibiotic susceptibility pattern and associated risk factors of urinary tract infection among clinically suspected children attending at Felege-Hiwot Comprehensive and Specialized Hospital, Northwest Ethiopia. A prospective study

2020 ◽  
Author(s):  
Adugna Fenta ◽  
Mulat Dagnew ◽  
Setegn Eshetie ◽  
Teshome Belachew
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Susceptibility ◽  
Northwest Ethiopia ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Gram Negative ◽  
Antimicrobial Susceptibility Pattern ◽  
Specialized Hospital

Abstract Background: Urinary tract infection is one of the most common bacterial infections in children. Understanding the characteristics of uropathogens and their antimicrobial susceptibility pattern in a particular setting can provide evidence for the appropriate management of cases. This study aimed to assess the bacterial profile of urinary tract infection, their antimicrobial susceptibility pattern and associated factors among clinically suspected children attending at Felege-Hiwot Comprehensive Specialized Hospital, Northwest Ethiopia.Methods: A hospital-based cross-sectional study was conducted from February-April, 2019. A systematic sampling technique was employed. A mid-stream urine sample was inoculated on cystine lactose electrolyte deficient media and incubated for 24-48 hours. Sub-culturing was done on Mac-Conkey and blood agar. Antimicrobial susceptibility test was done on Muller-Hinton agar. A binary logistic regression model was used to see the association between dependent and independent factors. A p-value<0.05 at 95% CI was considered as statistically significant.Results: The overall prevalence of urinary tract infection was 16.7% (95% CI 12.4-21.1). Both Gram-negative and Gram-positive bacterial isolates were recovered with a rate of 44/50 (88%) and 6/50 (12%) respectively. Among Gram-negative isolates, E. coli 28/44(63.6%) was predominant while S. saprophyticus 2/6(33.3%) was prevalent among Gram-positive bacterial isolates. Overall, a high level of resistance to ampicillin, Augmentin, and tetracycline was shown by Gram-negative bacteria with a rate of 44/44(100%), 39/44(88.6%), and36/44 (81.8%) respectively. About 33/50(66%) of overall multidrug resistance was observed (95% CI 52-78). About six Gram-negative bacterial isolates were extended spectrum beta-lactamase (ESBL) producers. Having a history of urinary tract infection (P-0.003, AOR 1.86-22.15) and male uncircumcision (p-0.00, AOR 5.5-65.35) were the independent variables that associate for urinary tract infections.

In vitro biofilm formation and antimicrobial susceptibility patterns of bacteria isolated from suspected external ocular infected patients attending Jimma University Medical Center eye clinic, southwest Ethiopia

2019 ◽  
Author(s):  
Kuma Diriba ◽  
Tesfaye Kassa ◽  
Yared Alemu ◽  
Sisay Bekele
Keyword(s):  
Antimicrobial Susceptibility ◽  
Biofilm Formation ◽  
Medical Center ◽  
Ocular Infection ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Southwest Ethiopia ◽  
Antimicrobial Susceptibility Pattern ◽  
Ocular Infections ◽  
Jimma University

Abstract Background: Ocular disease with its complications is a major public health problem which significantly impacts on quality of life in developing countries. An ocular infection due to microbial agents, can lead to reduced vision and blindness. This study was aimed to assess the antimicrobial susceptibility pattern and biofilm forming potential of bacteria isolated from suspected external ocular infected patients attending Jimma University Medical Center (JUMC). Method: A cross sectional facility based study was conducted on 319 suspect patients with external ocular infections from March 2017 to June 2017 at JUMC in Southwest Ethiopia. External ocular specimens were collected and standard operating procedures were followed to handle and culture throughout the study period. Antimicrobial susceptibility pattern of the isolates was determined by disk diffusion method according to CLSI 2015. Microtiter (96 wells) plate method was used to screen biofilm formation by measuring optical density at 570nm. Result: Out of 319 study participants with external ocular infection, the prevalence of bacterial pathogens was 46.1%. The predominant bacterial isolates were Coagulase negative staphylococcus (CoNS) (27.7%) followed by Staphylococcus aureus (19.7%). Among Gram negatives, Pseudomonas aeroginosa (6.8%) was the leading isolate. Increased antimicrobial resistance was observed for tetracycline (64%), erthromycin (66.7%) and penicillin (77.1%). Amoxicillin-clavulanic acid, ciprofloxacin and gentamicin were the most effective drugs for both Gram negative and Gram positive ranging from about 70 to 100% with the later two drugs for external ocular infections. Methicillin resistant S. aureus (MRSA) accounted for 13.8% of S. aureus isolates.. Multidrug resistance (MDR) accounted for 68.7%. The overall biofilm formation rate of bacterial ocular pathogens was 66.1%; with P. aeruginosa (40%), CoNS (34.1%) and S. aureus (31%) formed strong biofilm phenotype. Conclusion: The prevalence of bacterial isolates among external ocular infection was high. Almost all bacterial isolates were resistant to atleast one or more drugs. MDR pathogens were observed increasingly among biofilm formers or vice versa. Therefore, antimicrobial susceptibility testing should be practiced to guide treatment of external ocular cases and to control the emergence of drug resistant bacteria.

scholarly journals Antimicrobial Susceptibility Pattern of Bacterial Isolates from Wound Infection

2018 ◽  
Vol 26 ◽  
pp. 61-66
Author(s):  
S Gul Nahar ◽  
M Bulbul Hasan ◽  
M Nawshad Ali ◽  
Mst Rokeya Khatun ◽  
K Khanam
Keyword(s):  
Wound Infection ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Penicillin G ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Coagulase Negative Staphylococci ◽  
Antimicrobial Susceptibility Pattern ◽  
Gram Positive Bacteria

Objective: The present study was done to see the antimicrobial susceptibility pattern of bacterial isolates from wound infection.Methods: Wound swabs collected from 150 patients of wound infection were cultured and microbial isolates identified using standard methods. Antimicrobial susceptibility testing was done on bacterial isolates by Modified Kirby Bauer method.Results: Of the 150 swabs 131 (87.4%) were culture positive for bacterial pathogens, while 19 (12.6%) were bacteriologically sterile showing an isolation rate of 87.4%. The predominant bacteria isolated from the infected wounds were Staphylococcus aureus 47 (32.4%) followed by Escherichia coli 29 (20%), Proteus species 23 (16%), Coagulase negative Staphylococci 21 (14.5%), Klebsiella pneumoniae 14 (10%) and Pseudomonas aeruginosa 11 (8%).In case of gram positive bacteria- rate of isolates resistant to ampicillin was 94%, followed by penicillin G, 86.8%, Tetracycline, 51.5%. In case of gram negative bacteria- rate of isolates resistant to ampicillin was 96%, followed by cephalothin, 92.4%, Tetracycline, 74%.Conclusion: In antimicrobial susceptibility testing ampicillin, penicillin, cephalothin and tetracycline were the least effective. Gentamicin, norfloxacin, ciprofloxacin, vancomycin and amikacin were the most effective antibiotics.TAJ 2013; 26: 61-66

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