scholarly journals Biodegradation of high concentrations of benzene vapors in a two phase partition stirred tank bioreactor

2013 ◽  
Vol 10 (1) ◽  
Author(s):  
Ali Karimi ◽  
Farideh Golbabaei ◽  
Masoud Neghab ◽  
Mohammad Reza Pourmand ◽  
Ahmad Nikpey ◽  
...  
Keyword(s):  
Stirred Tank ◽  
Two Phase ◽  
Stirred Tank Bioreactor ◽  
Phase Partition ◽  
High Concentrations ◽  
Two Phase Partition

Selective Release of Serotonin from Human Platelets by X-537 A, Acting as an Ionophore for Serotonin

1975 ◽  
Author(s):  
P. Wörner ◽  
R. Brossmer
Keyword(s):  
Organic Phase ◽  
Adenine Nucleotides ◽  
Human Platelets ◽  
Cytoplasmic Calcium ◽  
Two Phase ◽  
Phase Partition ◽  
High Concentrations ◽  
Significant Uptake ◽  
Two Phase Partition ◽  
Deutsche Forschungsgemeinschaft

The ionophore X-537 A facilitates the permeation of calcium through membranes. Human platelets exposed to X-537 A undergo a release of serotonin, which has been attributed to an increase in the concentration of cytoplasmic calcium, mediated by X-537 A. However, at a low concentration of X-537 A, platelets release serotonin but one cannot observe a release of heparin-neutralizing activity (PF4) and adenine nucleotides, an activation of clot-promoting activity (PF3), or a significant uptake of 45calcium into platelets. Electron micrographs of platelets after exposure to X-537A do not exhibit the alterations known to be coupled with the release reaction, despite a considerable release of serotonin. Two-phase partition studies demonstrate that X-537A is able to extract serotonin into an organic phase. In summary, these results indicate that X-537A causes a selective release of serotonin by transporting it through membranes. Only at high concentrations of X-537A can one establish an uptake of 45calcium by platelets and a release of PF4 and adenine nucleotides in addition to that of serotonin.(Supported by “Deutsche Forschungsgemeinschaft, Bonn”, Sonderforschungsbereich 90, “Cardiovasculares System”.)

The application of aqueous two-phase partition to the study of chick limb mesenchymal diversification

Development ◽  
1986 ◽  
Vol 94 (1) ◽  
pp. 267-275
Author(s):  
C. P. Cottrill ◽  
Paul T. Sharpe ◽  
Lewis Wolpert
Keyword(s):  
Pattern Formation ◽  
Limb Development ◽  
Developmental Stages ◽  
Proximal Region ◽  
Limb Bud ◽  
Cell Populations ◽  
Two Phase ◽  
Phase Partition ◽  
Surface Character ◽  
Two Phase Partition

A technique which identifies cells differing in surface character, aqueous two-phase partition using thin-layer countercurrent distribution (TLCCD), has been used to study differentiation and pattern formation in the developing chick limb bud. The TLCCD profiles of cell populations, derived from various regions of morphologically undifferentiated mesenchyme from three different stages of limb development, have been compared. At no stage, or location, has the population been found to be homogeneous. Cells from progress zones and more proximal regions could all be resolved into several populations. The populations from progress zones at three different developmental stages were qualitatively similar but differed in the proportions of cells in each. The most striking differences in cell populations were those obtained from the most proximal region of the limb, closest to the flank, which represents the developmentally most advanced region.

Isolation of rat liver lysosomes by a single two-phase partition on dextran/polyethylene glycol

1990 ◽  
Vol 185 (2) ◽  
pp. 249-253 ◽  
Author(s):  
Jesús Osada ◽  
Hortensia Aylagas ◽  
José Sánchez-Prieto ◽  
Immaculada Sánchez-Vegazo ◽  
Evangelina Palacios-Alaiz
Keyword(s):  
Polyethylene Glycol ◽  
Rat Liver ◽  
Two Phase ◽  
Phase Partition ◽  
Liver Lysosomes ◽  
Two Phase Partition ◽  
Rat Liver Lysosomes
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