scholarly journals Determination of Suitable Housekeeping Genes for Normalisation of Quantitative Real Time PCR Analysis of Cells Infected with Human Immunodeficiency Virus and Herpes Viruses

2007 ◽  
Vol 4 (1) ◽  
pp. 130 ◽  
Author(s):  
Sarah Watson ◽  
Sarah Mercier ◽  
Chris Bye ◽  
John Wilkinson ◽  
Anthony Cunningham ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Housekeeping Genes ◽  
Pcr Analysis ◽  
Herpes Viruses ◽  
Quantitative Real Time Pcr ◽  
Immunodeficiency Virus

The validation of housekeeping genes as a reference in quantitative Real Time PCR analysis

Gene ◽  
2014 ◽  
Vol 549 (2) ◽  
pp. 280-285 ◽  
Author(s):  
Justyna Jarczak ◽  
Jarosław Kaba ◽  
Emilia Bagnicka
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Housekeeping Genes ◽  
Pcr Analysis ◽  
Quantitative Real Time Pcr

scholarly journals Evaluation of Housekeeping Genes for Quantitative Real-Time PCR Analysis of Bradysia odoriphaga (Diptera: Sciaridae)

2016 ◽  
Vol 17 (7) ◽  
pp. 1034 ◽  
Author(s):  
Caihua Shi ◽  
Fengshan Yang ◽  
Xun Zhu ◽  
Erxia Du ◽  
Yuting Yang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Housekeeping Genes ◽  
Pcr Analysis ◽  
Quantitative Real Time Pcr

scholarly journals Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae pv. oryzae--infection and melatonin supplementation

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Xian Chen ◽  
Pedro Laborda ◽  
Yan Dong ◽  
Fengquan Liu
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Housekeeping Genes ◽  
Suitable Reference Gene ◽  
Pcr Analysis ◽  
Quantitative Real Time Pcr ◽  
Rice Plants ◽  
Qrt Pcr ◽  
Suitable Reference

Abstract Exogenous melatonin (MT) was found to be an interesting tool for enhancing the resistance of rice to Xanthomonasoryzaepv. oryzae (Xoo)-caused bacterial blight (BB). However, the accurate comparison of the expression levels across samples was a challenging task. In this work, the stability of 10 common used housekeeping genes under Xoo-infection and MT supplementation in rice was analyzed using quantitative real-time PCR (qRT-PCR), and algorithms geNorm, NormFinder and BestKeeper. Our results indicated that most reference genes remained stable in Xoo-infected rice plants, while a number of reference genes were affected by MT supplementation. Among all studied genes, the transcript levels of 18S(18S ribosomal RNA) and UBC (Ubiquitin-conjugating enzyme E2) remained unaltered by Xoo infection, while UBC and UBQ5(Ubiquitin 5) were the most stable genes when examining simultaneous Xoo-infection and MT supplementation, demonstrating that UBC is a suitable reference gene for qRT-PCR data normalization in rice under Xoo-infection and MT supplementation.

scholarly journals Identification and Validation of Suitable Housekeeping Genes for Normalizing Quantitative Real-Time PCR Assays in Injured Peripheral Nerves

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e105601 ◽  
Author(s):  
Giovanna Gambarotta ◽  
Giulia Ronchi ◽  
Olivier Friard ◽  
Pantaleo Galletta ◽  
Isabelle Perroteau ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Peripheral Nerves ◽  
Housekeeping Genes ◽  
Quantitative Real Time Pcr ◽  
Pcr Assays
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