Phylogenetic relationship of the Brazilian isolates of the rat lungworm Angiostrongylus cantonensis (Nematoda: Metastrongylidae) employing mitochondrial COI gene sequence data

Zaprionus indianus (Gupta, 1970) (Diptera Drosophilidae), also known as African fig fly, isconsidered as the primary pest only to fig fruits along with secondary pest to other fruits. Its oc-currence has been reported from many countries but not previously reported from Bangladesh.With this note, we report here the first observation of Z. indianus from Bangladesh. Fly sampleswere collected by using yeast-banana traps. The species was identified by morphological char-acteristics and mitochondrial COI gene sequence.

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Genetic identification of Javan hawk-eagle (Nisaetus bartelsi) from Indonesia using mitochondrial COI gene

Asia Pacific Journal of Molecular Biology and Biotechnology ◽

10.35118/apjmbb.2019.027.2.10 ◽

2019 ◽

pp. 70-77

Author(s):

Suhadi ◽

Dwi Listyorini ◽

Riri Wiyanti Retnaningtyas ◽

Fima Rizki Eka Putri ◽

Dina Ayu Valentiningrum

Keyword(s):

Phylogenetic Tree ◽

Gene Sequence ◽

Dna Isolation ◽

Genetic Identification ◽

Coi Gene ◽

Mitochondrial Coi ◽

Mitochondrial Coi Gene ◽

Conservation Effort ◽

Interspecific Distance ◽

Intraspecific Distance

Nisaetus bartelsi is a native hawk from Java which its genetic information is not commonly understood yet. As a part of the conservation effort to maintain genetic diversity of this endangered species, this research aimed to obtain COI gene sequences from five individuals of N. bartelsi to confirm its position in the phylogenetic tree. DNA isolation from 5 N. bartelsi blood sample was performed and its COI gene sequence was amplified, sequenced, and used to reconstruct phylogenetic tree using MEGA6 with several other members of Nisaetus, Aquila, and Saggitaridae family. Furthermore, the intraspecific distance between 5 N. bartelsi samples and interspecific distance with other species were calculated using MEGA6. The result suggested that all five individuals belonged to the species Javan hawk-eagle (N. bartelsi) and were closely related to the Blyth’s hawk-eagle (Nisaetus alboniger). The DNA barcoding of the Javan hawk-eagle conducted in this study is a stepping stone to conservation efforts for the Javan hawk-eagle.

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Oziella sibirica (Acari: Eriophyoidea: Phytoptidae), a new eriophyoid mite species described using confocal microscopy, COI barcoding and 3D surface reconstruction

Zootaxa ◽

10.11646/zootaxa.3560.1.3 ◽

2012 ◽

Vol 3560 (1) ◽

pp. 41 ◽

Cited By ~ 12

Author(s):

PHILIPP E. CHETVERIKOV ◽

FRÉDÉRIC BEAULIEU ◽

TATJANA CVRKOVIĆ ◽

BILJANA VIDOVIĆ ◽

RADMILA U. PETANOVIĆ

Keyword(s):

Laser Scanning ◽

Gene Sequence ◽

Sequence Data ◽

Mite Species ◽

Laser Scanning Microscopy ◽

Coi Gene ◽

Confocal Laser ◽

Prodorsal Shield ◽

Mitochondrial Coi ◽

Internal Genitalia

Oziella sibirica sp. nov., collected from sedges (Cyperaceae: Carex macroura) in Siberia, Russia, is herein describedbased on the external morphology of all active instars using primarily conventional phase contrast microscopy, andon the female internal genitalia and prodorsal shield design using confocal laser scanning microscopy (CLSM)imaging and a 3D modelling technique. A partial mitochondrial COI gene sequence of O. sibirica sp. nov. is alsoprovided, through GenBank, and this represents the first published record of any gene sequence data for the familyPhytoptidae. We present remarks on the phylogenetic significance of the position of setae 3a in immature instars oferiophyoids and on the ontogenic variability of the empodium morphology of O. sibirica sp. nov. Using this speciesas a model, we propose a method for describing the internal genitalia of eriophyoids based on CLSM. We advocatethe use of CLSM imaging as a new, relatively simple technique for observing and describing the internal genitalia oferiophyoids, as these largely unexplored genitalic structures may provide phylogenetically meaningful informationfor improving the classification of this poorly understood group of mites. In addition, CLSM may complementconventional light microscopy techniques in facilitating the interpretation of external structures such as body ornamentation or chaetotaxy.

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