Genome sequence of the English grain aphid, Sitobion avenae and its endosymbiont Buchnera aphidicola

The English grain aphid, Sitobion avenae, is a major agricultural pest of wheat, barley and oats, and one of the principal vectors of Barley Yellow Dwarf Virus (BYDV) leading to significant reductions in grain yield, annually. Emerging resistance to and increasing regulation of insecticides has resulted in limited options for their control. Using PacBio HiFi data, we have produced a high quality draft assembly of the S. avenae genome; generating a primary assembly with a total assembly size of 475.7 Mb, and an alternate assembly with a total assembly size of 430.8 Mb. Our primary assembly was highly contiguous with only 326 contigs and a contig N50 of 15.95 Mb. Assembly completeness was estimated at 97.7% using BUSCO analysis and 31,007 and 29,037 protein coding genes were predicted from the primary and alternate assemblies, respectively. This assembly, which is to our knowledge the first for an insecticide resistant clonal lineage of English grain aphid, will provide novel insight into the molecular and mechanistic determinants of resistance and will facilitate future research into mechanisms of viral transmission and aphid behavior.

Impacts of Constitutive and Induced Benzoxazinoids Levels on Wheat Resistance to the Grain Aphid (Sitobion avenae)

Benzoxazinoids are important secondary metabolites in gramineae plants and have inhibitory and toxic effects against a wide range of herbivore pests. However, the relationship between benzoxazinoid level and plant resistance to aphids remains controversial. In this study, we investigated the relationship between benzoxazinoids composition and concentration in wheat leaves and the resistance to the grain aphid Sitobion avenae. Overall, six benzoxazinoids were detected and identified by mass spectrometry based metabolites profiling, including three lactams, two hydroxamic acids, and one methyl derivative. The constitutive levels of these benzoxazinoids were significantly different among the wheat varieties/lines. However, none of these benzoxazinoids exhibited considerable correlation with aphid resistance. S. avenae feeding elevated the level of 2-O-β-D-glucopyranosyl-oxy-4,7-dimethoxy-(2H)-1,4-benzoxazin-3(4H)-one (HDMBOA-Glc) and reduced the level of 2-O-β-D-glucopyranosyloxy-4-hydroxy-7-(2H)-methoxy-1,4-benzoxazin-3(4H)-one (DIMBOA-Glc) in some of the wheat varieties/lines. Moreover, aphid-induced level of DIMBOA-Glc was positively related with callose deposition, which was closely associated with aphid resistance. Wheat leaves infiltrated with DIMBOA-Glc caused a noticeable increase of callose deposition and the effect was in a dose dependent manner. This study suggests that the constitutive level of benzoxazinoids has limited impact on S. avenae. Aphid feeding can affect the balance of benzoxazinoids metabolism and the dynamic level of benzoxazinoids can act as a signal of callose deposition for S. avenae resistance. This study will extend our understanding of aphid–wheat interaction and provides new insights in aphid-resistance wheat breeding.

De novo assembly and comparative transcriptome analysis revealed the genes that were potentially involved in defensive terpenoids emission in the wheat against the wheat aphid Sitobion avenae (Fabricius)

The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.

Wheat–Fusarium graminearum Interactions Under Sitobion avenae Influence: From Nutrients and Hormone Signals

The English grain aphid Sitobion avenae and phytopathogen Fusarium graminearum are wheat spike colonizers. “Synergistic” effects of the coexistence of S. avenae and F. graminearum on the wheat spikes have been shown in agroecosystems. To develop genetic resistance in diverse wheat cultivars, an important question is how to discover wheat–F. graminearum interactions under S. avenae influence. In recent decades, extensive studies have typically focused on the unraveling of more details on the relationship between wheat-aphids and wheat-pathogens that has greatly contributed to the understanding of these tripartite interactions at the ecological level. Based on the scientific production available, the working hypotheses were synthesized from the aspects of environmental nutrients, auxin production, hormone signals, and their potential roles related to the tripartite interaction S. avenae–wheat–F. graminearum. In addition, this review highlights the relevance of preexposure to the herbivore S. avenae to trigger the accumulation of mycotoxins, which stimulates the infection process of F. graminearum and epidemic of Fusarium head blight (FHB) in the agroecosystems.

Nanoselenium Enhanced Wheat Resistance to Aphids by Regulating Biosynthesis of DIMBOA and Volatile Components

Aphid s are one of the most destructive insects in many cultivated plants including wheat, which can cause significant yield loss and damage the quality of agricultural products. Therefore, it is essential to control the occurrence of aphids during wheat growth. Previous studies reported the alterations in the resistance of wheat to pests induced by several external factors such as nutrients in soils and nano-carbonaceous materials. In this study, nanoselenium (nano-Se) was sprayed on wheat leaves at several concentration levels (1.0, 5.0, and 20 mg/L). Nano-Se (5.0 mg/L) could significantly reduce Sitobion avenae number (36%) compared with the control . The foliar application of nano-Se was found to enhance the antioxidation capacity by reducing MDA concentration and increasing GSH-Px, CAT, GSH, Pro and VE concentrations. Phenylpropane pathway was activated after the application of nano-Se, with significantly increasing apigenin and caffeic acid concentrations. The high-level expression of the related genes ( TaBx1A , TaBx3A , TaBx4A, TaASMT2, and TaCOMT ) induced the increasing melatonin concentration by 88.6% and DIMBOA concentration by 64.3%. Different ratios of the secondary metabolites to nano-Se were conducted to examine the effects on wheat resistance to the Sitobion avenae . The results revealed the combination of nano-Se and melatonin can achieve the best overall performance by reducing the Sitobion avenae infection by 52.2%. The results from this study suggest that the coordinative applications nano-Se and melatonin combination could be more effectively improve the wheat resistance to aphids via promotion of volatile organic compound synthesis and modulation in phenylpropane and indole metabolism pathways.