Oziella sibirica (Acari: Eriophyoidea: Phytoptidae), a new eriophyoid mite species described using confocal microscopy, COI barcoding and 3D surface reconstruction

Zootaxa ◽  
2012 ◽  
Vol 3560 (1) ◽  
pp. 41 ◽  
Author(s):  
PHILIPP E. CHETVERIKOV ◽  
FRÉDÉRIC BEAULIEU ◽  
TATJANA CVRKOVIĆ ◽  
BILJANA VIDOVIĆ ◽  
RADMILA U. PETANOVIĆ
Keyword(s):  
Laser Scanning ◽  
Gene Sequence ◽  
Sequence Data ◽  
Mite Species ◽  
Laser Scanning Microscopy ◽  
Coi Gene ◽  
Confocal Laser ◽  
Prodorsal Shield ◽  
Mitochondrial Coi ◽  
Internal Genitalia

Oziella sibirica sp. nov., collected from sedges (Cyperaceae: Carex macroura) in Siberia, Russia, is herein describedbased on the external morphology of all active instars using primarily conventional phase contrast microscopy, andon the female internal genitalia and prodorsal shield design using confocal laser scanning microscopy (CLSM)imaging and a 3D modelling technique. A partial mitochondrial COI gene sequence of O. sibirica sp. nov. is alsoprovided, through GenBank, and this represents the first published record of any gene sequence data for the familyPhytoptidae. We present remarks on the phylogenetic significance of the position of setae 3a in immature instars oferiophyoids and on the ontogenic variability of the empodium morphology of O. sibirica sp. nov. Using this speciesas a model, we propose a method for describing the internal genitalia of eriophyoids based on CLSM. We advocatethe use of CLSM imaging as a new, relatively simple technique for observing and describing the internal genitalia oferiophyoids, as these largely unexplored genitalic structures may provide phylogenetically meaningful informationfor improving the classification of this poorly understood group of mites. In addition, CLSM may complementconventional light microscopy techniques in facilitating the interpretation of external structures such as body ornamentation or chaetotaxy.

First pentasetacid mite from Australasian Araucariaceae: morphological description and molecular phylogenetic position of Pentasetacus novozelandicus n. sp. (Eriophyoidea, Pentasetacidae) and remarks on anal lobes in eriophyoid mites

2019 ◽  
Vol 24 (7) ◽  
pp. 1284-1309 ◽  
Author(s):  
Philipp Chetverikov ◽  
C. CRAEMER C. CRAEMER ◽  
T. CVRKOVIĆ T. CVRKOVIĆ ◽  
P.G. EFIMOV P.G. EFIMOV ◽  
P.B. KLIMOV P.B. KLIMOV ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Phylogenetic Analyses ◽  
Mite Species ◽  
Laser Scanning Microscopy ◽  
Coi Gene ◽  
Confocal Laser ◽  
Phylogenetic Position ◽  
Morphological Description ◽  
Eriophyoid Mites ◽  
Molecular Phylogenetic

A new vagrant eriophyoid mite species of the archaic genus Pentasetacus (Schliesske 1985), P. novozelandicus n. sp., is described with the aid of conventional microscopy, confocal laser scanning microscopy and scanning electron microscopy. It was found on Araucaria heterophylla, which is an araucarian that is endemic to Norfolk Island and introduced to New Zealand. Partial sequences of mitochondrial barcode COI gene and D1–D2 domains of nuclear rDNA of two pentasetacid mites, P. araucariae (MK903025 and MK898944) and P. novozelandicus n. sp. (MK903024 and MK898943) are provided. Molecular phylogenetic analyses of full-length D1–D2 eriophyoid sequences, including GenBank sequences and newly generated sequences of pentasetacids, confirmed the monophyly of Pentasetacidae but failed to resolve the basal phylogeny of Eriophyoidea. This may be because the D1–D2 domains of 28S are hypervariable in Eriophyoidea. Moreover, in pentasetacids D1–D2 sequences are about 20% shorter than in other eriophyoids, and thus harder to align. Two types of anal lobes are described in Eriophyoidea: (1) Eriophyidae s.l. and Phytoptidae s.l. have bilaterally symmetric lobes; (2) pentasetacids have non-divided lobes. The presence of an anal secretory apparatus, comprising internal structures that have previously been described in Eriophyidae s.l. and Phytoptidae s.l., is confirmed in pentasetacid genera. The phylogeny of pentasetacids is also discussed in the context of the paleobiography of Araucariaceae.

Redescription of P. dorsipapillatus (von Marenzeller, 1893) and additional comments on the other species in the genus Pholoides Pruvot, 1895 (Polychaeta: Sigalionidae)

Zootaxa ◽  
2018 ◽  
Vol 4450 (1) ◽  
pp. 91
Author(s):  
KATHARINA HINTZE ◽  
KARIN MEIßNER ◽  
ANDREAS BICK
Keyword(s):  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Type Material ◽  
Confocal Laser ◽  
Small Scale ◽  
Sequence Information ◽  
Small Species ◽  
Mitochondrial Coi ◽  
Excellent Tool ◽  
Scanning Electron

Pholoides Pruvot, 1895 is a species-poor genus of small scale-bearing polychaetes. Pholoides species are restricted to the continental shelf, living in sandy and muddy substrates, or on hard bottoms. During the DIVA 3 expedition grab samples were taken on four seamounts of the Meteor Seamount complex. One of the most common polychaetes in these samples was P. dorsipapillatus (von Marenzeller, 1893). Based on this material a comparison with the type material is undertaken leading to a re-description of P. dorsipapillatus. Diagnostic characters of all accepted Pholoides species were evaluated using light, scanning electron and confocal laser scanning microscopy. CLSM in particular proved to be an excellent tool for investigating these small species, and especially the type material. Blossom-like sensory buds, found at certain appendages of P. dorsipapillatus, could be an important diagnostic character to distinguish P. dorsipapillatus from other Pholoides species. Sequence information on three different gene fragments, the mitochondrial COI and 16S and the nuclear H3a, were obtained, and could serve for future phylogenetic and phylogeographic studies. 

The anal secretory apparatus of Eriophyoidea and description of Phyllocoptes bilobospinosus n. sp. (Acariformes: Eriophyidae) from Tamarix (Tamaricaceae) from Ukraine, Crimea and USA 

2019 ◽  
Vol 24 (1) ◽  
pp. 139
Author(s):  
Philipp Chetverikov ◽  
Samuel J. Bolton ◽  
Alexander I. Gubin ◽  
Viktoria Yu. Letukhova ◽  
Andrey E. Vishnyakov ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
New Combination ◽  
Careful Examination ◽  
Confocal Laser ◽  
Prodorsal Shield ◽  
Secretory Structures ◽  
Anal Glands ◽  
Large Spike ◽  
Secretory Apparatus

A new vagrant phyllocoptine species, Phyllocoptes bilobospinosus n. sp. (Eriophyidae, Phyllocoptinae), found on tamarisks (Tamarix tetrandra Pallas, T. smyrnensis Bunge, T. ramossisima Ledeb) in Donbass (Ukraine), Crimea, and USA is described based on conventional light microscopy and confocal laser scanning microscopy. Apart from two distinct areas of ventral cuticle bearing large, spike-like microtubercles, the new species possesses a thin translucent supracapitular plate (situated below the frontal lobe of the prodorsal shield), a short longitudinal ventral ridge anterior to the anal lobes, and unusual internal tube-like structures associated with the rectum. Careful examination of purposefully made slide mounts of partially cleared specimens revealed that adults of P. bilobospinosus possess a complex of structures associated with the rectum, including a hypertrophied, four-lobed putative anal gland and four thin tubes connected with a rectal sac. Similar tubular structures previously described in aberoptine mites of the genus Aberoptus from Brazilian Cesalpiniaceae are discussed. The synonymy of genera Aberoptus Keifer and Aceria Keifer is rejected and a new combination, Aberoptus inusitatus (Britto & Navia (in Britto et al. 2008)) n. comb., is proposed. A brief review of the anal glands of Eriophyoidea is given, including a discussion on homology and the variety of forms of the anal secretory apparatus among eriophyoid genera. Further research is needed on the anatomy of anal glands in Eriophyoidea, including transmission electron microscopy based histological analyses and additional studies of eriophyoids with well-developed secretory structures associated with the rectum. These methods will lead to a much better understanding of the evolution and homology of the anal secretory apparatus, which may render it useful for future phylogenetic studies.

An integrative description of a new Cephalothrix species (Nemertea: Palaeonemertea) from the South China Sea

Zootaxa ◽  
2021 ◽  
Vol 4908 (4) ◽  
pp. 584-594
Author(s):  
ALEXEI V. CHERNYSHEV ◽  
NEONILA E. POLYAKOVA
Keyword(s):  
Laser Scanning ◽  
18S Rrna ◽  
Laser Scanning Microscopy ◽  
Colour Pattern ◽  
Confocal Laser ◽  
28S Rrna ◽  
Body Colour ◽  
Mitochondrial Coi ◽  
The Family ◽  
Molecular Phylogenetic

A new nemertean species of the genus Cephalothrix from intertidal calcareous red algae off the Vietnam coast is described based on histological sections, confocal laser scanning microscopy, and nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, H3) and two mitochondrial (COI, 16S rRNA) DNA fragments. Cephalothrix suni sp. nov. is characterized by a unique body colour pattern consisting of transverse brown on ventral body side. A molecular phylogenetic analysis of the family Cephalotrichidae using a multigene approach has identified three clades for Cephalothrix: Cephalothrix, Procephalothrix, and interstitial cephalotrichids. The new species belongs to clade Procephalothrix sensu Chernyshev & Kajihara 2019 and a subclade of species with red, orange, or dark yellow anterior tip. 

scholarly journals Recent speciation and hybridization in Icelandic deep-sea isopods: an integrative approach using genomics and proteomics

2021 ◽  
Author(s):  
Eva Paulus ◽  
Saskia Brix ◽  
Annabelle Siebert ◽  
Pedro Martínez Arbizu ◽  
Sven Rossel ◽  
...  
Keyword(s):  
Gene Flow ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Integrative Approach ◽  
Confocal Laser ◽  
Hybrid Zones ◽  
Genetic Lineages ◽  
Mitochondrial Coi ◽  
Genomics And Proteomics ◽  
Wide Range

The isopod species Haploniscus bicuspis (G.O. Sars, 1877) shows circum-Icelandic distribution in a wide range of environmental conditions and along well-known geographic barriers, such as the Greenland-Iceland-Faroe (GIF) Ridge. We wanted to explore population genetics, phylogeography and cryptic speciation as well as to investigate whether previously described, but unaccepted subspecies have any merit. Using the same set of specimens, we combined mitochondrial COI sequences, thousands of nuclear loci (ddRAD), and proteomic profiles, plus selected morphological characters using Confocal Laser Scanning Microscopy (CLSM). Five divergent genetic lineages were identified by COI and ddRAD, two south and three north of the GIF Ridge. Assignment of populations to the three northern lineages varied and detailed analyses revealed hybridization and gene flow between them, suggesting a single northern species with a complex phylogeographic history. No apparent hybridization was observed among lineages south of the Ridge, inferring the existence of two more species. Differences in proteomic profiles between the three putative species were minimal, implying an ongoing or recent speciation process. Population differentiation was high, even among closely associated populations, and higher in mitochondrial COI than nuclear ddRAD loci. Gene flow is apparently male-biased, leading to hybrid zones and instances of complete exchange of the local nuclear genome through immigrating males. This study did not confirm the existence of subspecies defined by male characters, which probably characterize different male developmental stages present in all species.

Resolving the phylogenetic position of enigmatic New Guinea and Seychelles Scutigeromorpha (Chilopoda): a molecular and morphological assessment of Ballonemini

2010 ◽  
Vol 24 (6) ◽  
pp. 539 ◽  
Author(s):  
Aodhán D. Butler ◽  
Gregory D. Edgecombe ◽  
Alexander D. Ball ◽  
Gonzalo Giribet
Keyword(s):  
Laser Scanning ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
New Guinea ◽  
Sister Group ◽  
Laser Scanning Microscopy ◽  
Morphological Data ◽  
Confocal Laser ◽  
Phylogenetic Position ◽  
Sister Group Relationship

Recent phylogenetic analyses of scutigeromorph centipedes omitted New Guinea endemics for lack of modern data, either from morphology or molecular sequences. Among these is the tribe Ballonemini, originally established for Ballonema Verhoeff, 1904, and Parascutigera Verhoeff, 1904, based on similar tergal prominences. Subsequent systematic revision led to their separation into different subfamilies. Combined analyses of morphology and sequence data including Ballonema gracilipes Verhoeff, 1904, resolve Ballonema either in a grade of Scutigerinae or as sister to all other Scutigerinae + Thereuoneminae. Confocal laser scanning microscopy (CLSM) of the types of B. gracilipes demonstrates the utility of this technique for non-destructive imaging of historical museum material at a resolution comparable to scanning electron microscopy. A possible record of Ballonema in the Seychelles is dismissed; a collection from Silhouette samples a member of Thereuoneminae described as Seychellonema gerlachi, gen. nov. sp. nov. Morphological data, analysed with sequence data for other Scutigeromorpha, ally Seychellonema with the Oriental–Australian genus Thereuopoda Verhoeff, 1904, but it displays a novel patterning of its tergal spinula and tarsal papillae. The phylogenetic analyses include sequence data for African Pselliodidae, corroborating a sister group relationship to remaining Scutigeromorpha and generating a more stable result than in earlier analyses using only Neotropical species.

Confocal laser scanning microscopy technique for the study of internal genitalia and external morphology of eriophyoid mites (Acari: Eriophyoidea)

Zootaxa ◽  
2012 ◽  
Vol 3453 (1) ◽  
pp. 56 ◽  
Author(s):  
PHILIPP E. CHETVERIKOV
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Blue Laser ◽  
Laser Scanning Microscopy ◽  
External Morphology ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Eriophyoid Mites ◽  
Internal Genitalia

Confocal laser scanning microscopy (CLSM) is a modern powerful technique that can be used for studying the externaland internal anatomy of arthropods. CLSM has seldom been used in acarology and very rarely for studying eriophyoidmites. It allows the capture of precise digital images of the fine details of external and internal chitinous structures, whichcan be further analysed using various computer programs. CLSM can serve as an effective tool for comparing closelyrelated and/or cryptic species, correcting diagnoses of poorly described taxa, studying immature instars, and particularly,for studying the structures and the functioning of the internal genitalia of adult females and males. In this paper, thepotential use of CLSM for the study of eriophyoids is demonstrated using specimens of 13 mite species and eight generafrom the families Phytoptidae Murray 1877 and Eriophyidae Nalepa 1898. This study showed that freshly mountedspecimens on microscope slides appeared to be the most appropriate for CLSM as older specimens tended to have reducedautofluorescence. The best choice for studying the external morphology and internal genital apparatus of eriophyoid mitesappeared to be the blue laser. Green and light blue wavelengths (488 nm and 532 nm) were found to be less useful. Thequality of CLSM images depended on the slide-mounting medium used. Among those compared, Hoyer’s medium wasfound to be the most appropriate whereas Heinze medium and media including Iodium gave poorer results. The empodiaand proximal parts of setae were shown to have very weak autofluorescence signals, but they reflected red (635 nm) and blue (405 nm) laser light, which could be detected with CLSM.

Supplementary description of five species from the genus Cecidophyopsis (Eriophyoidea: Eriophyidae: Cecidophyinae)

2019 ◽  
Vol 24 (8) ◽  
pp. 1555-1578
Author(s):  
Slavica Marinković ◽  
Philipp Chetverikov ◽  
Tatjana Cvrković ◽  
Biljana Vidović ◽  
Radmila Petanović
Keyword(s):  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Taxus Baccata ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Ilex Aquifolium ◽  
Internal Genitalia ◽  
Corylus Avellana L ◽  
First Time ◽  
Mitochondrial Cytochrome Oxidase

Supplementary morphological descriptions of five Cecidophyopsis species collected in Europe (Serbia, Austria, Italy and Montenegro) are given: Cecidophyopsis vermiformis from Corylus avellana L. (Betulaceae), C. hendersoni from Yucca gigantea Lem. (Asparagaceae), C. verilicis from Ilex aquifolium L. (Aquifoliaceae), C. psilaspis from Taxus baccata L. (Taxaceae) and C. malpighianus from Laurus nobilis L. (Lauraceae). Males of C. vermiformis, C. verilicis, C. hendersoni and C. malpighianus, and immatures of C. hendersoni and C. verilicis, are described for the first time. C. verilicis is recorded for the first time in the fauna of Serbia and the European region. Female cuticle-lined internal genitalia of five Cecidophyopsis species are studied under confocal laser scanning microscopy. A several steps of oviposition in cecidophyines is proposed based on CLSM observations on their internal genitalia. Sequences of the barcoding region of the mitochondrial cytochrome oxidase subunit I (mtCOI) gene are given for the following species: C. hendersoni, C. verilicis, C. psilaspis and C. malpighianus.

scholarly journals Size, shape, and direction matters: Matching secondary genital structures in male and female mites using multiple microscopy techniques and 3D modeling

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0254974
Author(s):  
Orlando Cómbita-Heredia ◽  
Connor J. Gulbronson ◽  
Ronald Ochoa ◽  
Edwin Javier Quintero-Gutiérrez ◽  
Gary Bauchan ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Sperm Transfer ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Anatomical Structures ◽  
Internal Structures ◽  
Internal Genitalia ◽  
Temperature Scanning ◽  
Microscopy Techniques ◽  
Quality Surface

Studies of female genital structures have generally lagged behind comparable studies of male genitalia, in part because of an assumption of a lower level of variability, but also because internal genitalia are much more difficult to study. Using multiple microscopy techniques, including video stereomicroscopy, fluorescence microscopy, low-temperature scanning electron microscopy (LT-SEM), and confocal laser scanning microscopy (CLSM) we examined whether the complex sperm transfer structures in males of Megalolaelaps colossus (Acari: Mesostigmata) are matched by similarly complex internal structures in the female. While both LT-SEM and CLSM are well suited for obtaining high-quality surface images, CLSM also proved to be a valuable technique for observing internal anatomical structures. The long and coiled sperm transfer organ on the chelicera of the males (spermatodactyl) largely matches an equally complex, but internal, spiral structure in the females in shape, size, and direction. This result strongly suggests some form of genital coevolution. A hypothesis of sexual conflict appears to provide the best fit for all available data (morphology and life history).

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