scholarly journals Effect of combinations of marketed human anthelmintic drugs against Trichuris muris in vitro and in vivo

2012 ◽  
Vol 5 (1) ◽  
Author(s):  
Jennifer Keiser ◽  
Lucienne Tritten ◽  
Roberto Adelfio ◽  
Mireille Vargas
Keyword(s):  
Trichuris Muris ◽  
Anthelmintic Drugs

scholarly journals Botanicals: a natural approach to control ascaridiosis in poultry

2018 ◽  
Vol 69 (1) ◽  
pp. 711 ◽  
Author(s):  
I. SYMEONIDOU ◽  
E. BONOS ◽  
K. MOUSTAKIDIS ◽  
P. FLOROU-PANERI ◽  
E. CHRISTAKI ◽  
...  
Keyword(s):  
Economic Losses ◽  
Meat Production ◽  
Ascaridia Galli ◽  
Natural Approach ◽  
The Past ◽  
Poultry Products ◽  
Anthelmintic Efficacy ◽  
Anthelmintic Drugs

Parasites (protozoa, helminthes, arthropods) represent a main threat for poultry worldwide. Among helminthes, nematodes constitute the most important group of parasites of poultry. The nematode Ascaridia galli, the cause of ascaridiosis in poultry, is one of the most important and prevalent parasites, resulting in serious economic losses, associated with the treatment cost, the decreased feed efficiency, and the poor egg and meat production. During the past few decades the indiscriminate use of anthelmintic drugs has generated several cases of resistance in helminthes in poultry, situation which is coupled with the severity of residues in poultry products. For this reason, nowadays attention has been drawn to the use of botanicals in poultry diet, due to their anthelmintic properties. Furthermore, the dietary use eco-friend ly of these plant derived substances compared to conventional synthetic anthelmintic drugs is considered as a natural and ecofriendly approach by the consumers. The focus of the present review is to recapitulate the studies, both in vivo and in vitro, that have demonstrated the anthelmintic efficacy of various dietary botanicals in controlling poultry ascaridiosis.

scholarly journals Anthelmintic action of plant cysteine proteinases against the rodent stomach nematode,Protospirura muricola,in vitroandin vivo

Parasitology ◽  
2006 ◽  
Vol 134 (1) ◽  
pp. 103-112 ◽  
Author(s):  
G. STEPEK ◽  
A. E. LOWE ◽  
D. J. BUTTLE ◽  
I. R. DUCE ◽  
J. M. BEHNKE
Keyword(s):  
Detrimental Effect ◽  
Gastrointestinal Nematodes ◽  
Cysteine Proteinases ◽  
Trichuris Muris ◽  
Kiwi Fruit ◽  
Heligmosomoides Polygyrus ◽  
Anthelmintic Efficacy ◽  
Papaya Latex

Cysteine proteinases from the fruit and latex of plants, including papaya, pineapple and fig, were previously shown to have a rapid detrimental effect,in vitro, against the rodent gastrointestinal nematodes,Heligmosomoides polygyrus(which is found in the anterior small intestine) andTrichuris muris(which resides in the caecum). Proteinases in the crude latex of papaya also showed anthelmintic efficacy against both nematodesin vivo. In this paper, we describe thein vitroandin vivoeffects of these plant extracts against the rodent nematode,Protospirura muricola, which is found in the stomach. As in earlier work, all the plant cysteine proteinases examined, with the exception of actinidain from the juice of kiwi fruit, caused rapid loss of motility and digestion of the cuticle, leading to death of the nematodein vitro. In vivo, in contrast to the efficacy againstH. polygyrusandT. muris, papaya latex only showed efficacy againstP. muricolaadult female worms when the stomach acidity had been neutralized prior to administration of papaya latex. Therefore, collectively, our studies have demonstrated that, with the appropriate formulation, plant cysteine proteinases have efficacy against nematodes residing throughout the rodent gastrointestinal tract.

scholarly journals In vitro anthelmintic effects of cysteine proteinases from plants against intestinal helminths of rodents

2007 ◽  
Vol 81 (4) ◽  
pp. 353-360 ◽  
Author(s):  
Gillian Stepek ◽  
Ann E. Lowe ◽  
David J. Buttle ◽  
Ian R. Duce ◽  
Jerzy M. Behnke
Keyword(s):  
Rapid Development ◽  
Intestinal Helminths ◽  
Cysteine Proteinases ◽  
Trichuris Muris ◽  
Heligmosomoides Polygyrus ◽  
Development Of Resistance ◽  
Taxonomic Groups ◽  
Gi Helminths

AbstractInfections with gastrointestinal (GI) nematodes are amongst the most prevalent worldwide, especially in tropical climates. Control of these infections is primarily through treatment with anthelmintic drugs, but the rapid development of resistance to all the currently available classes of anthelmintic means that alternative treatments are urgently required. Cysteine proteinases from plants such as papaya, pineapple and fig are known to be substantially effective against three rodent GI nematodes, Heligmosomoides polygyrus, Trichuris muris and Protospirura muricola, both in vitro and in vivo. Here, based on in vitro motility assays and scanning electron microscopy, we extend these earlier reports, demonstrating the potency of this anthelmintic effect of plant cysteine proteinases against two GI helminths from different taxonomic groups – the canine hookworm, Ancylostoma ceylanicum, and the rodent cestode, Rodentolepis microstoma. In the case of hookworms, a mechanism of action targeting the surface layers of the cuticle indistinguishable from that reported earlier appears to be involved, and in the case of cestodes, the surface of the tegumental layers was also the principal location of damage. Hence, plant cysteine proteinases have a broad spectrum of activity against intestinal helminths (both nematodes and cestodes), a quality that reinforces their suitability for development as a much-needed novel treatment against GI helminths of humans and livestock.

scholarly journals Identification of Novel Genes in Intestinal Tissue That Are Regulated after Infection with an Intestinal Nematode Parasite

2005 ◽  
Vol 73 (7) ◽  
pp. 4025-4033 ◽  
Author(s):  
R. Datta ◽  
M. L. deSchoolmeester ◽  
C. Hedeler ◽  
N. W. Paton ◽  
A. M. Brass ◽  
...  
Keyword(s):  
Trna Synthetase ◽  
Tryptophan Metabolism ◽  
Trichuris Muris ◽  
Local Responses ◽  
Intestinal Nematode ◽  
Expression Of Genes ◽  
Regulated Expression ◽  
Microarray Analyses

ABSTRACT Infection of resistant or susceptible mice with Trichuris muris provokes mesenteric lymph node responses which are polarized towards Th2 or Th1, respectively. These responses are well documented in the literature. In contrast, little is known about the local responses occurring within the infected intestine. Through microarray analyses, we demonstrate that the gene expression profile of infected gut tissue differs according to whether the parasite is expelled or not. Genes differentially regulated postinfection in resistant BALB/c mice include several antimicrobial genes, in particular, intelectin (Itln). In contrast, analyses in AKR mice which ultimately progress to chronic infection provide evidence for a Th1-dominated mucosa with up-regulated expression of genes regulated by gamma interferon. Increases in the expression of genes associated with tryptophan metabolism were also apparent with the coinduction of tryptophanyl tRNA synthetase (Wars) and indoleamine-2,3-dioxygenase (Indo). With the emerging literature on the role of these gene products in the suppression of T-cell responses in vitro and in vivo, their up-regulated expression here may suggest a role for tryptophan metabolism in the parasite survival strategy.

Approved oncology drugs lack in vivo activity against Trichuris muris despite in vitro activity

2016 ◽  
Vol 115 (11) ◽  
pp. 4443-4446 ◽  
Author(s):  
Noemi Cowan ◽  
Alessia Raimondo ◽  
Jennifer Keiser
Keyword(s):  
Vitro Activity ◽  
In Vitro Activity ◽  
Trichuris Muris

Anthelmintic Effect of Date Palm Fruit: A Systematic Review

2018 ◽  
Vol 17 (3) ◽  
pp. 276-281
Author(s):  
A. Rauf ◽  
F.H. Addnan ◽  
N.F.M. Manzor ◽  
W.O. Abdullah ◽  
M.A. Elkadi ◽  
...  
Keyword(s):  
Systematic Review ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Economic Losses ◽  
Trichuris Muris ◽  
Palm Fruit ◽  
Parasitic Helminths ◽  
Anthelmintic Effect

Soil-transmitted helminth infections constitute a global affliction affecting people and livestock, resulting in a significant disease burden and economic losses in endemic regions. However, control has not been entirely successful and the problem is compounded by the development of resistance to available chemotherapy. Novel alternative compounds are urgently needed, encouraging the screening of largely plant-based sources for anthelmintic effects. Date palm fruit (Phoenix dactylifera) is a popular and nutritious food much coveted for its nutraceutical effect. There are convincing reports documenting the antibacterial, antiviral and antifungal effects of date palm fruit but evidence for any potential anthelmintic effect remains unclear. Here we conducted a systematic review of the published information through an electronic search using six scientific databases; SCOPUS, MEDLINE COMPLETE, OVID, EMBASE, WEB OF SCIENCE and CINAHL COMPLETE. Out of 58 studies that were initially identified only 2 manuscripts fitted our inclusion criteria. The first article reported on the anthelmintic effect of date palm fruit against the rodent nematodes Trichuris muris and Angiostrongylus cantonensis in vitro and in vivo while the second article was based only on in vivo assessment (with coconut) of the effect on the rodent cestode Hymenolepis microstoma. Our review revealed limited anecdotal reports of the anthelmintic effect of date palm fruit against parasitic helminths, however, the majority of reports were inconclusive due to mixed results, limited focus on date palm fruit and lack of any statistical analyses. Rigorous, systematic and fully controlled studies focusing on date palm fruit are needed to validate the anecdotal evidence.

scholarly journals Benzimidazole and Aminoalcohol Derivatives Show in Vitro Anthelmintic Activity Against Trichuris Muris and Heligmosomoides Polygyrus

2022 ◽  
Author(s):  
Elora Valderas-García ◽  
Cécile Häberli ◽  
María Álvarez Bardón ◽  
Nerea Escala ◽  
Verónica Castilla Gómez de Agüero ◽  
...  
Keyword(s):  
Adult Stage ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Economic Losses ◽  
Parasitic Infections ◽  
Trichuris Muris ◽  
Heligmosomoides Polygyrus ◽  
Derivatives Of

Abstract Background: Infections by gastrointestinal nematodes cause significant economic losses and disease in both human and animals worldwide. The discovery of novel anthelmintic drugs is a crucial point in maintaining control of these parasitic infections.Methods: For this purpose, the aim of the present study was to evaluate the potential anthelmintic activity of three series of compounds against the gastrointestinal nematodes Trichuris muris and Heligmosomoides polygyrusin vitro. The compounds tested are derivatives of benzimidazole, lipidic aminoalcohols and diamines. A primary screening was performed to select those compounds with an ability to inhibit T. muris L1 motility by more than 90% at a single concentration of 100 µM, and then, their respective IC50s were calculated. Those compounds with IC50 lower than 10 µM were also tested against the adult stage of T. muris and H. polygyrus at a single concentration of 10µM.Results: Of the 41 initial compounds screened, only compounds AO14, BZ6 and BZ12 had IC50s lower than 10 µM on T. muris L1 assay, showing IC50 values of 3.30, 8.89 and 4.17 µM, respectively. However, only two of them displayed activity against the adult stage of the parasites: BZ12 killed 81% of adults of T. muris (IC50 of 8.1 µM) and 53% of H. polygyrus while BZ6 killed 100% of H. polygyrus adults (IC50 of 5.3 µM) but only 17% of T. muris. Conclusions: BZ6 and BZ12 could be considered as potential candidates for further in vivo efficacy testing.

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