scholarly journals Ectopic expression of a grape nitrate transporter VvNPF6.5 improves nitrate content and nitrogen use efficiency in Arabidopsis

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Yani He ◽  
Xiaojun Xi ◽  
Qian Zha ◽  
Yuting Lu ◽  
Aili Jiang
Keyword(s):  
Nitrogen Use Efficiency ◽  
Nitrate Uptake ◽  
Functional Characterization ◽  
Ectopic Expression ◽  
Nitrate Content ◽  
Nitrate Transporter ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
Ph Dependent ◽  
Nitrate Response

Abstract Background Nitrate plays an important role in grapevines vegetative and reproductive development. However, how grapevines uptake, translocate and utilize nitrate and the molecular mechanism still remains to be investigated. Results In this study, we report the functional characterization of VvNPF6.5, a member of nitrate transporter 1/peptide transporter family (NRT1/PTR/NPF) in Vitis vinifera. Subcellular localization in Arabidopsis protoplasts indicated that VvNPF6.5 is plasma membrane localized. Quantitative RT-PCR analysis indicated that VvNPF6.5 is expressed predominantly in roots and stems and its expression is rapidly induced by nitrate. Functional characterization using cRNA-injected Xenopus laevis oocytes showed that VvNPF6.5 uptake nitrate in a pH dependent way and function as a dual-affinity nitrate transporter involved in both high- and low-affinity nitrate uptake. Further ectopic expression of VvNPF6.5 in Arabidopsis resulted in more 15NO3− accumulation in shoots and roots and significantly improved nitrogen use efficiency (NUE). Moreover, VvNPF6.5 might participate in the nitrate signaling by positively regulating the expression of primary nitrate response genes. Conclusion Our results suggested that VvNPF6.5 encodes a pH-dependent, dual-affinity nitrate transporter. VvNPF6.5 regulates nitrate uptake and allocation in grapevines and is involved in primary nitrate response.

scholarly journals Genome-wide associated study identifies NAC42-activated nitrate transporter conferring high nitrogen use efficiency in rice

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Weijie Tang ◽  
Jian Ye ◽  
Xiangmei Yao ◽  
Pingzhi Zhao ◽  
Wei Xuan ◽  
...  
Keyword(s):  
Nitrogen Use Efficiency ◽  
Negative Impact ◽  
Nitrate Transporter ◽  
High Nitrogen ◽  
Rice Varieties ◽  
Nitrogen Use ◽  
Genome Wide ◽  
Related Transcription Factor ◽  
Use Efficiency ◽  
Natural Variations

AbstractOver-application of nitrogen fertilizer in fields has had a negative impact on both environment and human health. Domesticated rice varieties with high nitrogen use efficiency (NUE) reduce fertilizer for sustainable agriculture. Here, we perform genome-wide association analysis of a diverse rice population displaying extreme nitrogen-related phenotypes over three successive years in the field, and identify an elite haplotype of nitrate transporter OsNPF6.1HapB that enhances nitrate uptake and confers high NUE by increasing yield under low nitrogen supply. OsNPF6.1HapB differs in both the protein and promoter element with natural variations, which are differentially trans-activated by OsNAC42, a NUE-related transcription factor. The rare natural allele OsNPF6.1HapB, derived from variation in wild rice and selected for enhancing both NUE and yield, has been lost in 90.3% of rice varieties due to the increased application of fertilizer. Our discovery highlights this NAC42-NPF6.1 signaling cascade as a strategy for high NUE and yield breeding in rice.

scholarly journals Natural variation of nitrate uptake and nitrogen use efficiency in Arabidopsis thaliana cultivated with limiting and ample nitrogen supply

2010 ◽  
Vol 61 (9) ◽  
pp. 2293-2302 ◽  
Author(s):  
Fabien Chardon ◽  
Julien Barthélémy ◽  
Françoise Daniel-Vedele ◽  
Céline Masclaux-Daubresse
Keyword(s):  
Arabidopsis Thaliana ◽  
Nitrogen Use Efficiency ◽  
Natural Variation ◽  
Nitrate Uptake ◽  
Nitrogen Supply ◽  
Nitrogen Use ◽  
Use Efficiency

scholarly journals Nitrogen and Sulphur Fertilisation for Marketable Yields of Cabbage (Brassica oleracea L. var. Capitata), Leaf Nitrate and Glucosinolates and Nitrogen Losses Studied in a Field Experiment in Central Slovenia

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1304
Author(s):  
Nina Kacjan Maršić ◽  
Ksenija Sinkovič Može ◽  
Rok Mihelič ◽  
Marijan Nečemer ◽  
Metka Hudina ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Nitrogen Use Efficiency ◽  
Temperate Climate ◽  
Nitrate Content ◽  
Nitrogen Use ◽  
Calcium Ammonium Nitrate ◽  
Nitrogen Fertilisation ◽  
Aliphatic Glucosinolates ◽  
Use Efficiency ◽  
Central Slovenia

A field trial of white cabbage (Brassica oleracea var. Capitata L.) was carried out under the humid temperate climate conditions in Central Slovenia to investigate the effects of calcium ammonium nitrate (0, 180 and 240 kg N ha−1) and gypsum (0 and 40 kg S ha−1) fertilisation on yield, yield quality (nitrate, glucosinolate levels and glucosinolate profile) and nitrogen use efficiency. The highest marketable yield, dry matter yield and nitrogen uptake were obtained at the highest nitrogen fertilisation rate when in combination with sulphur. For this treatment, the nitrogen surplus in the soil after harvesting was lower than for the same nitrogen fertilisation without sulphur application. For the combination N240S40, the sulphur addition significantly increased nitrogen use efficiency, which resulted in reduced nitrate content in the cabbage heads. The chemical forms of glucosinolates showed that 80–85% were aliphatic glucosinolates with the remainder as the indole group. For the aliphatic glucosinolates, significant interactions between nitrogen and sulphur fertilisations were reflected in increased levels of progoitrin and glucoiberin when sulphur was applied at the lower nitrogen fertilisation rates. For the indole group, the levels of glucobrassicin and the indole group itself decreased at higher nitrogen fertilisation rates, independent of sulphur fertilisation.

scholarly journals Members of BTB gene family regulate negatively nitrate uptake and nitrogen use efficiency in Arabidopsis thaliana and Oryza sativa

2016 ◽  
pp. pp.01731.2015 ◽  
Author(s):  
Viviana Araus ◽  
Elena A Vidal ◽  
Tomas Puelma ◽  
Simon Alamos ◽  
Delphine Mieulet ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Oryza Sativa ◽  
Gene Family ◽  
Nitrogen Use Efficiency ◽  
Nitrate Uptake ◽  
Nitrogen Use ◽  
Use Efficiency

scholarly journals Nitrate uptake and its regulation in relation to improving nitrogen use efficiency in cereals

2018 ◽  
Vol 74 ◽  
pp. 97-104 ◽  
Author(s):  
Darren C. Plett ◽  
Luke R. Holtham ◽  
Mamoru Okamoto ◽  
Trevor P. Garnett
Keyword(s):  
Nitrogen Use Efficiency ◽  
Nitrate Uptake ◽  
Nitrogen Use ◽  
Use Efficiency

Reducing the nitrate content of burley tobacco by grafting with flue-cured tobacco

2021 ◽  
Author(s):  
Yuqing Feng ◽  
Yuanyuan Zhao ◽  
Geng Li ◽  
Yafei Li ◽  
Jun Zhou ◽  
...  
Keyword(s):  
Nitrogen Metabolism ◽  
Nitrogen Use Efficiency ◽  
Soluble Sugar ◽  
Nitrate Content ◽  
High Nitrogen ◽  
Nitrogen Use ◽  
Chlorophyll Metabolism ◽  
Tobacco Leaves ◽  
Burley Tobacco ◽  
Use Efficiency

Abstract Background: Nitrosition of tobacco pyridine alkaloids by nitrate-derived NOx is the origin of tobacco-specifc nitrosamines (TSNAs) formed in tobacco, which are among the most notable toxicants present in tobacco products and smoke. Burley tobacco is particularly susceptible to TSNA formation because the cultivars exhibit a chloroplast-deficient and nitrogen-use-defciency phenotype which results in high accumulation of nitrate. Strategies to lower nitrate levels in tobacco could produce a corresponding decrease in TSNAs accumulation in leaves. Previous studies have showed that grafting with high nitrogen use efficiency rootstock was able to improve the nitrogen utilization of flue-cured tobacco. In this study, a reciprocativel grafting experiment has been conducted with two varieties of different tobacco types (burley tobacco ‘Eyan No.1’ and flue-cured tobacco ‘K326’) to investigate whether replacing burley tobacco root with flue-cured tobacco by grafting can enhance pigment biosynthesis and photosynthesis parameters and reduce nitrate content of burley tobacco leaves, and to explore the corresponding mechanism. Results: The results showed that compared with the self-rooted burley tobacco seedlings, grafting significantly increased the pigment content, net photosynthesis, biomass, total soluble sugar, reducing sugar, nitrate reductase activity, glutamine synthetase activities, NH4-N and soluble protein content of the leaves of Eyan No.1 while decreased the nitrate content and NO3-N/TN. Interestingly, transcription level analysis suggested that replacing burley tobacco root with flue-cured tobacco by grafting was able to up-regulate the genes involved in starch and sucrose metabolism, porphyrin and chlorophyll metabolism, carbon fixation in photosynthetic organism metabolism, carotenoid biosynthesis and nitrogen metabolism of burley tobacco leaves. In addition, the PPI network revealed gene_68511, gene_35043 and gene_77508 had higher degrees via replacing burley tobacco root with flue-cured tobacco, which might be hub proteins to reduce nitrate accumulation. Conclusions: In conclusion, grafting with high nitrogen use efficiency rootstock provided an exceptionally promising means of nitrate reduction in burley tobacco leaf , which is a principle precursor of TSNAs, due to the improvement of photosynthesis and nitrogen metabolism in the scion.

scholarly journals Genome-wide Analysis of NPF, NRT2, CLC and SLAC1/SLAH Nitrate Transporters in Hexaploid Wheat (Triticum Aestivum)

2021 ◽  
Author(s):  
Aman Kumar ◽  
Nitika Sandhu ◽  
Pankaj Kumar ◽  
Gomsie Pruthi ◽  
Jasneet Singh ◽  
...  
Keyword(s):  
Transport System ◽  
Nitrogen Use Efficiency ◽  
Hexaploid Wheat ◽  
Peptide Transporter ◽  
Cereal Crops ◽  
Synthetic Hexaploid Wheat ◽  
Nitrate Transporter ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
Nitrate Transporters

Abstract Nitrogen transport is one of the most important processes in plants mediated by specialized transmembrane proteins. Plants have two main systems for nitrogen uptake from soil and its transport within the system - a low-affinity transport system and a high-affinity transport system. Nitrate transporters are of special interest in cereal crops because large amount of money is spent on N fertilizers every year to enhance the crop productivity. Till date four gene families of nitrate transporter proteins; NPF (nitrate transporter 1/peptide transporter family), NRT2 (nitrate transporter 2 family), the CLC (chloride channel family), and the SLAC/SLAH (slow anion channel-associated homologues) have been reported in plants. In our study, in silico mining of nitrate transporter genes along with their detailed structure, phylogenetic and expression analysis was carried out. A total of 412 nitrate transporter genes were identified in hexaploid wheat genome using HMMER based homology searches in IWGSC Refseq v2.0. Out of those twenty genes were root specific, 11 leaf/shoot specific and 17 genes were grain/spike specific. The identification of nitrate transporter genes in the close proximity to the previously identified 67 marker-traits associations associated with the nitrogen use efficiency related traits in nested synthetic hexaploid wheat introgression library indicated the robustness of the reported transporter genes. The detailed crosstalk between the genome and proteome and the validation of identified putative candidate genes through expression and gene editing studies may lay down the foundation to improve nitrogen use efficiency of cereal crops.

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