Investigation of cell wall proteins of C. sinensis leaves by combining cell wall proteomics and N-glycoproteomics

Abstract Background C. sinensis is an important economic crop with fluoride over-accumulation in its leaves, which poses a serious threat to human health due to its leaf consumption as tea. Recently, our study has indicated that cell wall proteins (CWPs) probably play a vital role in fluoride accumulation/detoxification in C. sinensis. However, there has been a lack in CWP identification and characterization up to now. This study is aimed to characterize cell wall proteome of C. sinensis leaves and to develop more CWPs related to stress response. A strategy of combined cell wall proteomics and N-glycoproteomics was employed to investigate CWPs. CWPs were extracted by sequential salt buffers, while N-glycoproteins were enriched by hydrophilic interaction chromatography method using C. sinensis leaves as a material. Afterwards all the proteins were subjected to UPLC-MS/MS analysis. Results A total of 501 CWPs and 195 CWPs were identified respectively by cell wall proteomics and N-glycoproteomics profiling with 118 CWPs in common. Notably, N-glycoproteomics is a feasible method for CWP identification, and it can enhance CWP coverage. Among identified CWPs, proteins acting on cell wall polysaccharides constitute the largest functional class, most of which might be involved in cell wall structure remodeling. The second largest functional class mainly encompass various proteases related to CWP turnover and maturation. Oxidoreductases represent the third largest functional class, most of which (especially Class III peroxidases) participate in defense response. As expected, identified CWPs are mainly related to plant cell wall formation and defense response. Conclusion This was the first large-scale investigation of CWPs in C. sinensis through cell wall proteomics and N-glycoproteomics. Our results not only provide a database for further research on CWPs, but also an insight into cell wall formation and defense response in C. sinensis.

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A Survey on Cell wall Proteins of C. Sinensis Leaf by Combining Cell Wall Proteomic and N-Glycoproteomic Strategy

10.21203/rs.3.rs-132373/v1 ◽

2020 ◽

Author(s):

yanli liu ◽

Linlong Ma ◽

Dan Cao ◽

Ziming Gong ◽

Jing Fan ◽

...

Keyword(s):

Cell Wall ◽

Defense Response ◽

Large Scale ◽

Plant Cell Wall ◽

Wall Structure ◽

Cell Wall Proteins ◽

Cell Wall Formation ◽

Cell Wall Polysaccharides ◽

Wall Formation ◽

Cell Wall Proteome

Abstract Background: Camellia sinensis is an important economic crop with fluoride over-accumulation in the leaves, which pose a serious threaten to human health due to its leave being used for making tea. Recently, our study found that cell wall proteins (CWPs) probably play a vital role in fluoride accumulation/detoxification in C. sinensis. However, CWPs identification and characterization were lacking up to now in C. sinensis. Herein, we aimed at characterizing cell wall proteome of C. sinensis leaves, to develop more CWPs related to stress response. A strategy of combined cell wall proteome and N-glycoproteome were employed to investigate CWPs. CWPs were extracted by sequential salt buffers, while N-glycoproteins were enriched by hydrophilic interaction chromatography method using C. sinensis leaves as a material, afterwards all proteins were subjected to qualitative analysis via UPLC-MS/MS.Results: 501 and 195 CWPs were identified by cell wall proteomic and N-glycoproteomics profiling, respectively, with 118 CWPs being in common. Notably, N-glycoproteome is a feasible method for CWPs identification and consequently enhance CWP coverage. Among identified CWPs, proteins acting on cell wall polysaccharides constitute the largest functional group with most of them possibly being involved in the remodeling of cell wall structure. The second abundant group encompass mainly various proteases, being considered to be related to CWPs turnover and maturation. Oxidoreductases represent the third abundance with most of them especially Class III peroxidases being known to be implicated in defense response. As expected, identified CWPs emphasized on plant cell wall formation and defense response.Conclusion: This was the first large scale survey of CWPs by cell wall proteome and N-glycoproteome in C. sinensis. The results not only provides a database that will aid deep research on CWPs, but also improve the understanding underlying cell wall formation and defense response in this important economic specie.

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Role of cytoskeleton in plant cell wall formation

Scientia Sinica Vitae ◽

10.1360/ssv-2019-0168 ◽

2020 ◽

Vol 50 (2) ◽

pp. 176-186

Author(s):

Yi MAN ◽

RuiLi LI ◽

YuFen BU ◽

Na SUN ◽

YanPing JING ◽

...

Keyword(s):

Cell Wall ◽

Plant Cell ◽

Plant Cell Wall ◽

Cell Wall Formation ◽

Wall Formation

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WD40-Repeat Proteins in Plant Cell Wall Formation: Current Evidence and Research Prospects

Frontiers in Plant Science ◽

10.3389/fpls.2015.01112 ◽

2015 ◽

Vol 6 ◽

Cited By ~ 8

Author(s):

Gea Guerriero ◽

Jean-Francois Hausman ◽

Inés Ezcurra

Keyword(s):

Cell Wall ◽

Plant Cell ◽

Plant Cell Wall ◽

Current Evidence ◽

Cell Wall Formation ◽

Wd40 Repeat ◽

Wall Formation ◽

Repeat Proteins

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Defensive forwards: stress-responsive proteins in cell walls of crop plants

10.1101/2020.02.15.950535 ◽

2020 ◽

Cited By ~ 3

Author(s):

Liangjie Niu ◽

Wei Wang

Keyword(s):

Cell Wall ◽

Stress Responses ◽

Plant Cell Wall ◽

Strong Interactions ◽

Wall Structure ◽

Cell Wall Proteins ◽

Go Annotation ◽

Gene Differential Expression ◽

Expression Atlas ◽

Specific Proteins

ABSTRACTAs the vital component of plant cell wall, proteins play important roles in stress response through modifying wall structure and involving in wall integrity signaling. However, the potential of cell wall proteins (CWPs) in improvement of crop stress tolerance has probably been underestimated. Recently, we have critically reviewed the predictors, databases and cross-referencing of subcellular locations of possible CWPs in plants (Briefings in Bioinformatics 2018;19:1130-1140). In this study, taking maize (Zea mays) as an example, we retrieved 1873 entries of probable maize CWPs recorded in UniProtKB. As a result, 863 maize CWPs are curated and classified as 59 kinds of protein families. By referring to GO annotation and gene differential expression in Expression Atlas, we highlight the potential of CWPs as defensive forwards in abiotic and biotic stress responses. In particular, several CWPs are found to play key roles in adaptation to many stresses. String analysis also reveals possibly strong interactions among many CWPs, especially those stress-responsive enzymes. The results allow us to narrow down the list of CWPs to a few specific proteins that could be candidates to enhance maize resistance.

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Demonstration of A Distinct Pathway for Polar Exocytosis for Plant Cell Wall Formation

PLANT PHYSIOLOGY ◽

10.1104/pp.16.00754 ◽

2016 ◽

pp. pp.00754.2016 ◽

Cited By ~ 4

Author(s):

Liwen Jiang ◽

Hao Wang ◽

Xiaohoang Zhuang ◽

Xiangfeng Wang ◽

Ho Yin Law ◽

...

Keyword(s):

Cell Wall ◽

Plant Cell ◽

Plant Cell Wall ◽

Cell Wall Formation ◽

Wall Formation

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One, Two, Three: A Bioorthogonal Triple Labelling Strategy for Studying the Dynamics of Plant Cell Wall Formation In Vivo

Angewandte Chemie ◽

10.1002/ange.201808493 ◽

2018 ◽

Vol 130 (51) ◽

pp. 16907-16913 ◽

Cited By ~ 2

Author(s):

Clemence Simon ◽

Cedric Lion ◽

Corentin Spriet ◽

Fabien Baldacci‐Cresp ◽

Simon Hawkins ◽

...

Keyword(s):

Cell Wall ◽

Plant Cell ◽

Plant Cell Wall ◽

Cell Wall Formation ◽

Wall Formation

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Model systems for the understanding of lignified plant cell wall formation

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology ◽

10.1080/11263500500056559 ◽

2005 ◽

Vol 139 (1) ◽

pp. 93-97 ◽

Cited By ~ 6

Author(s):

B Cathala ◽

C Rondeau-Mouro ◽

D Lairez ◽

F Bedos Belval ◽

H Durand ◽

...

Keyword(s):

Cell Wall ◽

Plant Cell ◽

Plant Cell Wall ◽

Model Systems ◽

Cell Wall Formation ◽

Wall Formation

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The origin and early evolution of tracheids in vascular plants: integration of palaeobotanical and neobotanical data

Philosophical Transactions of the Royal Society B Biological Sciences ◽

10.1098/rstb.2000.0620 ◽

2000 ◽

Vol 355 (1398) ◽

pp. 857-868 ◽

Cited By ~ 59

Author(s):

William E. Friedman ◽

Martha E. Cook

Keyword(s):

Cell Wall ◽

Vascular Plants ◽

Secondary Cell Wall ◽

Early Evolution ◽

Land Plants ◽

Primary Cell Wall ◽

Wall Structure ◽

Cell Wall Formation ◽

Wall Formation ◽

Resistant Layer

Although there is clear evidence for the establishment of terrestrial plant life by the end of the Ordovician, the fossil record indicates that land plants remained extremely small and structurally simple until the Late Silurian. Among the events associated with this first major radiation of land plants is the evolution of tracheids, complex water–conducting cells defined by the presence of lignified secondary cell wall thickenings. Recent palaeobotanical analyses indicate that Early Devonian tracheids appear to possess secondary cell wall thickenings composed of two distinct layers: a degradation–prone layer adjacent to the primary cell wall and a degradation–resistant (possibly lignified) layer next to the cell lumen. In order to understand better the early evolution of tracheids, developmental and comparative studies of key basal (and potentially plesiomorphic) extant vascular plants have been initiated. Ultra–structural analysis and enzyme degradation studies of wall structure (to approximate diagenetic alterations of fossil tracheid structure) have been conducted on basal members of each of the two major clades of extant vascular plants: Huperzia (Lycophytina) and Equisetum (Euphyllophytina). This research demonstrates that secondary cell walls of extant basal vascular plants include a degradation–prone layer (‘template layer’) and a degradation–resistant layer (‘resistant layer’). This pattern of secondary cell wall formation in the water–conducting cells of extant vascular plants matches the pattern of wall thickenings in the tracheids of early fossil vascular plants and provides a key evolutionary link between tracheids of living vascular plants and those of their earliest fossil ancestors. Further studies of tracheid development and structure among basal extant vascular plants will lead to a more precise reconstruction of the early evolution of water–conducting tissues in land plants, and will add to the current limited knowledge of spatial, temporal and cytochemical aspects of cell wall formation in tracheary elements of vascular plants.

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