scholarly journals The activation of pyrin domain-containing-3 inflammasome depends on lipopolysaccharide from Porphyromonas gingivalis and extracellular adenosine triphosphate in cultured oral epithelial cells

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Wei Guo ◽  
Peng Wang ◽  
Zhonghao Liu ◽  
Pishan Yang ◽  
Ping Ye
Keyword(s):  
Epithelial Cells ◽  
Adenosine Triphosphate ◽  
Porphyromonas Gingivalis ◽  
Extracellular Adenosine ◽  
Oral Epithelial Cells ◽  
Pyrin Domain ◽  
Oral Epithelial

scholarly journals Identification of bistable populations of Porphyromonas gingivalis that differ in epithelial cell invasion

Microbiology ◽  
2010 ◽  
Vol 156 (10) ◽  
pp. 3052-3064 ◽  
Author(s):  
S. Suwannakul ◽  
G. P. Stafford ◽  
S. A. Whawell ◽  
C. W. I. Douglas
Keyword(s):  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Cell Invasion ◽  
Protease Activity ◽  
Periodontal Pathogen ◽  
Wild Type ◽  
Oral Epithelial Cells ◽  
Specific Protease ◽  
Oral Epithelial ◽  
First Time

Bistable populations of bacteria give rise to two or more subtypes that exhibit different phenotypes. We have explored whether the periodontal pathogen Porphyromonas gingivalis exhibits bistable invasive phenotypes. Using a modified cell invasion assay, we show for the first time that there are two distinct subtypes within a population of P. gingivalis strains NCTC 11834 and W50 that display differences in their ability to invade oral epithelial cells. The highly invasive subtype invades cells at 10–30-fold higher levels than the poorly invasive subtype and remains highly invasive for approximately 12–16 generations. Analysis of the gingipain activity of these subtypes revealed that the highly invasive type had reduced cell-associated arginine-specific protease activity. The role of Arg-gingipain activity in invasion was verified by enhancement of invasion by rgpAB mutations and by inclusion of an Arg-gingipain inhibitor in invasion assays using wild-type bacteria. In addition, a population of ΔrgpAB bacteria did not contain a hyperinvasive subtype. Screening of the protease activity of wild-type populations of both strains identified high and low protease subtypes which also showed a corresponding reduction or enhancement, respectively, of invasive capabilities. Microarray analysis of these bistable populations revealed a putative signature set of genes that includes oxidative stress resistance and iron transport genes, and which might be critical to invasion of or survival within epithelial cells.

scholarly journals A role for fimbriae in Porphyromonas gingivalis invasion of oral epithelial cells.

1997 ◽  
Vol 65 (5) ◽  
pp. 1980-1984 ◽  
Author(s):  
T Njoroge ◽  
R J Genco ◽  
H T Sojar ◽  
N Hamada ◽  
C A Genco
Keyword(s):  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Oral Epithelial Cells ◽  
Oral Epithelial

scholarly journals Porphyromonas gingivalis activates NFκB and MAPK pathways in human oral epithelial cells

2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Sabine Groeger ◽  
Fabian Jarzina ◽  
Eugen Domann ◽  
Joerg Meyle
Keyword(s):  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Oral Epithelial Cells ◽  
Mapk Pathways ◽  
Oral Epithelial

Porphyromonas gingivalis stimulates IL-6 and IL-8 secretion in GMSM-K, HSC-3 and H413 oral epithelial cells

Anaerobe ◽  
2014 ◽  
Vol 28 ◽  
pp. 62-67 ◽  
Author(s):  
Michael Yee ◽  
Shawn Kim ◽  
Pushpinder Sethi ◽  
Nejat Düzgüneş ◽  
Krystyna Konopka
Keyword(s):  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Oral Epithelial Cells ◽  
Oral Epithelial

Porphyromonas gingivalis FDC381 multiplies and persists within human oral epithelial cells in vitro.

1996 ◽  
Vol 64 (2) ◽  
pp. 660-664 ◽  
Author(s):  
P N Madianos ◽  
P N Papapanou ◽  
U Nannmark ◽  
G Dahlén ◽  
J Sandros
Keyword(s):  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Oral Epithelial Cells ◽  
Oral Epithelial
Sign in / Sign up

Export Citation Format

Share Document