scholarly journals Inhibition of Urokinase-Type Plasminogen Activator Expression by Macelignan in Porphyromonas gingivalis Supernatant-Induced Human Oral Epithelial Cells

2010 ◽  
Vol 17 (1) ◽  
pp. 31-37
Author(s):  
YANTI
Keyword(s):  
Epithelial Cells ◽  
Plasminogen Activator ◽  
Porphyromonas Gingivalis ◽  
Oral Epithelial Cells ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator ◽  
Oral Epithelial

Polarized secretion of urokinase-type plasminogen activator by epithelial cells

1992 ◽  
Vol 203 (1) ◽  
pp. 236-243 ◽  
Author(s):  
Pia Ragno ◽  
Anne Estreicher ◽  
Arnaud Gos ◽  
Annelise Wohlwend ◽  
Dominique Belin ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Plasminogen Activator ◽  
Polarized Secretion ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator

Retinal Pigment Epithelial Cells Secrete Urokinase-Type Plasminogen Activator and Its Inhibitor PAI-1

1992 ◽  
Vol 24 (4) ◽  
pp. 203-212 ◽  
Author(s):  
Vappu Sirén ◽  
Ross W. Stephens ◽  
Eeva-Marjatta Salonen ◽  
Antti Vaheri ◽  
Paula Summanen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Plasminogen Activator ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Pigment Epithelial ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator ◽  
Pai 1 ◽  
Pigment Epithelial Cells

scholarly journals Identification of bistable populations of Porphyromonas gingivalis that differ in epithelial cell invasion

Microbiology ◽  
2010 ◽  
Vol 156 (10) ◽  
pp. 3052-3064 ◽  
Author(s):  
S. Suwannakul ◽  
G. P. Stafford ◽  
S. A. Whawell ◽  
C. W. I. Douglas
Keyword(s):  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Cell Invasion ◽  
Protease Activity ◽  
Periodontal Pathogen ◽  
Wild Type ◽  
Oral Epithelial Cells ◽  
Specific Protease ◽  
Oral Epithelial ◽  
First Time

Bistable populations of bacteria give rise to two or more subtypes that exhibit different phenotypes. We have explored whether the periodontal pathogen Porphyromonas gingivalis exhibits bistable invasive phenotypes. Using a modified cell invasion assay, we show for the first time that there are two distinct subtypes within a population of P. gingivalis strains NCTC 11834 and W50 that display differences in their ability to invade oral epithelial cells. The highly invasive subtype invades cells at 10–30-fold higher levels than the poorly invasive subtype and remains highly invasive for approximately 12–16 generations. Analysis of the gingipain activity of these subtypes revealed that the highly invasive type had reduced cell-associated arginine-specific protease activity. The role of Arg-gingipain activity in invasion was verified by enhancement of invasion by rgpAB mutations and by inclusion of an Arg-gingipain inhibitor in invasion assays using wild-type bacteria. In addition, a population of ΔrgpAB bacteria did not contain a hyperinvasive subtype. Screening of the protease activity of wild-type populations of both strains identified high and low protease subtypes which also showed a corresponding reduction or enhancement, respectively, of invasive capabilities. Microarray analysis of these bistable populations revealed a putative signature set of genes that includes oxidative stress resistance and iron transport genes, and which might be critical to invasion of or survival within epithelial cells.

scholarly journals A role for fimbriae in Porphyromonas gingivalis invasion of oral epithelial cells.

1997 ◽  
Vol 65 (5) ◽  
pp. 1980-1984 ◽  
Author(s):  
T Njoroge ◽  
R J Genco ◽  
H T Sojar ◽  
N Hamada ◽  
C A Genco
Keyword(s):  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Oral Epithelial Cells ◽  
Oral Epithelial
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