scholarly journals OmpA protein sequence-based typing and virulence-associated gene profiles of Pasteurella multocida isolates associated with bovine haemorrhagic septicaemia and porcine pneumonic pasteurellosis in Thailand

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Teerasak E-kobon ◽  
Ratiporn Leeanan ◽  
Saengtian Pannoi ◽  
Pornchai Anuntasomboon ◽  
Pacharee Thongkamkoon ◽  
...  
Keyword(s):  
Protein Sequence ◽  
Pasteurella Multocida ◽  
Haemorrhagic Septicaemia ◽  
Pneumonic Pasteurellosis ◽  
Gene Profiles ◽  
Ompa Protein

scholarly journals Mass spectrometric analysis of lipid A obtained from the lipopolysaccharide of Pasteurella multocida

RSC Advances ◽  
2020 ◽  
Vol 10 (51) ◽  
pp. 30917-30933
Author(s):  
Abdul Tawab ◽  
Noor Akbar ◽  
Mujtaba Hasssan ◽  
Fazale Habib ◽  
Aamir Ali ◽  
...  
Keyword(s):  
Causative Agent ◽  
Pasteurella Multocida ◽  
Lipid A ◽  
Water Buffalo ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Type B ◽  
Haemorrhagic Septicaemia

LC/MS-based variant profiling of lipid A component of endotoxic lipopolysaccharides of Pasteurella multocida type B:2, a causative agent of haemorrhagic septicaemia in water buffalo and cattle.

scholarly journals First detection of Pasteurella multocida type B:2 in Hungary associated with systemic pasteurellosis in backyard pigs

2015 ◽  
Vol 63 (2) ◽  
pp. 141-156 ◽  
Author(s):  
Barbara Ujvári ◽  
Levente Szeredi ◽  
László Pertl ◽  
Gergely Tóth ◽  
Károly Erdélyi ◽  
...  
Keyword(s):  
Small Area ◽  
Pasteurella Multocida ◽  
Sequence Type ◽  
Type B ◽  
Chain Reaction ◽  
Haemorrhagic Septicaemia ◽  
Gene Typing ◽  
Polymerase Chain ◽  
Serotype B ◽  
Backyard Pigs

This is the first report of Pasteurella multocida type B in Hungarian pigs. This disease was observed in backyard-raised pigs in three households within a small area. Neither the source of the infection nor the epidemiological connection between any of the premises could be determined. The most consistent lesion was dark red discolouration of the skin of the ventral neck and brisket, with accompanying oedema and haemorrhages. The morbidity was low and lethality relatively high, with three dead (50%) and two euthanised (33%) out of six affected animals. A total of three isolates of P. multocida (P55, P56 and P57) were cultured from these cases and examined in detail. These were identified as P. multocida ssp. multocida biovar 3. All were toxA negative and belonged to serotype B:2. Multilocus sequence typing was used to assign these to a new sequence type (ST61) that is closely related to other haemorrhagic septicaemia causing strains of P. multocida regardless of the host. M13 polymerase chain reaction and virulence-associated gene typing also show that type B strains form a highly homogeneous, distinct phylogenic group within P. multocida.

scholarly journals Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector?

2021 ◽  
Vol 9 (9) ◽  
pp. 1999
Author(s):  
Peter Kutzer ◽  
Claudia A. Szentiks ◽  
Sabine Bock ◽  
Guido Fritsch ◽  
Tibor Magyar ◽  
...  
Keyword(s):  
Canis Lupus ◽  
Animal Species ◽  
Virulence Gene ◽  
Capsular Type ◽  
Type B ◽  
Haemorrhagic Septicaemia ◽  
The Core ◽  
Gene Profiles ◽  
First Time

Since 2010, outbreaks of haemorrhagic septicaemia (HS) caused by Pasteurella (P.) multocida capsular type B (PmB) emerged in Germany. In 2017, we noticed a close spatiotemporal relationship between HS outbreak sites and wolf (Canis lupus) territories. Thus, the main objectives of our study were to investigate the molecular epidemiology of German PmB-HS-isolates and to assess the role of wolves as putative vectors of this pathogen. We collected 83 PmB isolates from HS outbreaks that occurred between 2010 and 2019 and sampled 150 wolves, which were found dead in the years 2017 to 2019, revealing another three PmB isolates. A maximum-likelihood-based phylogeny of the core genomes of 65 PmB-HS-isolates and the three PmB-wolf-isolates showed high relatedness. Furthermore, all belonged to capsular:LPS:MLST genotype B:L2:ST122RIRDC and showed highly similar virulence gene profiles, but clustered separately from 35 global ST122RIRDC strains. Our data revealed that German HS outbreaks were caused by a distinct genomic lineage of PmB-ST122 strains, hinting towards an independent, ongoing epidemiologic event. We demonstrated for the first time, that carnivores, i.e., wolves, might harbour PmB as a part of their oropharyngeal microbiota. Furthermore, the results of our study imply that wolves can carry the pathogen over long distances, indicating a major role of that animal species in the ongoing epidemiological event of HS in Germany.

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