Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection

The viral capsid protein of the Seto virus (SeV), a Japanese strain of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like particles using a baculovirus expression system. An antigen detection enzyme-linked immunosorbent assay based on hyperimmune antisera to recombinant SeV was highly specific to homologous SeV-like strains but not heterologous strains in stools, allowing us type-specific detection of NLVs.

Download Full-text

Detection of Antibodies against Norovirus Genogroup GIV in Carnivores

Clinical and Vaccine Immunology ◽

10.1128/cvi.00312-09 ◽

2009 ◽

Vol 17 (1) ◽

pp. 180-182 ◽

Cited By ~ 20

Author(s):

Barbara Di Martino ◽

Fulvio Marsilio ◽

Federica Di Profio ◽

Eleonora Lorusso ◽

Klaus G. Friedrich ◽

...

Keyword(s):

Capsid Protein ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Specific Antibodies ◽

Capsid Protein Vp1

ABSTRACT Noroviruses (NoVs) resembling human NoV genotype GIV (Alphatron-like) have recently been detected in carnivores. By using an enzyme-linked immunosorbent assay based on baculovirus-expressed capsid protein VP1 of lion strain GGIV.2/Pistoia/387/06/ITA, NoV-specific antibodies were detected in cats (16.11%) and dogs (4.8%), demonstrating that these animals are exposed to infections caused by NoVs.

Download Full-text

Development and Application of a Double-Antigen Sandwich Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Porcine Circovirus 2

Clinical and Vaccine Immunology ◽

10.1128/cvi.00234-12 ◽

2012 ◽

Vol 19 (9) ◽

pp. 1480-1486 ◽

Cited By ~ 8

Author(s):

Meng Ge ◽

Wei Luo ◽

Daliang Jiang ◽

Runcheng Li ◽

Wenwei Zhao ◽

...

Keyword(s):

Capsid Protein ◽

Immunosorbent Assay ◽

Sandwich Elisa ◽

Test Procedure ◽

Porcine Circovirus ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Swine Industry ◽

Elisa Kit ◽

Porcine Circovirus 2

ABSTRACTA double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) is described for detection of porcine circovirus 2 (PCV2) antibodies using the well-characterized recombinant PCV2 capsid protein. In a comparative test of 394 pig sera against an indirect immunofluorescence (IIF) test and a commercial ELISA kit (also based on the recombinant PCV2 capsid protein), the results showed that the diagnostic sensitivity, specificity, and accuracy of the assay were, respectively, 90.61, 94.02, and 91.62% compared with IIF and 94.38, 95.28, and 94.67% compared with the commercial ELISA kit. Assay of 12 PCV-free pigs over a 5-week period produced only PCV2-negative titers by all 3 methods. These results and the seroprofiles of 4 pig farms obtained by both the commercial ELISA kit and the double-antigen sandwich ELISA indicate that the sandwich ELISA is a reliable method for detection of antibodies to PCV2. Additionally, the method described here permits the use of undiluted test serum samples simultaneously loaded with horseradish peroxidase (HRP)-conjugated antigen into the test well, and the complete test procedure can be performed in less than 90 min. This double-antigen sandwich ELISA should be a useful tool to aid swine industry professionals in deciding the intervention strategies for the control of PCV2-associated diseases.

Download Full-text