scholarly journals Molecular characterization of lumpy skin disease virus (LSDV) emerged in Bangladesh reveals unique genetic features compared to contemporary field strains

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Shukes Chandra Badhy ◽  
Mohammad Golam Azam Chowdhury ◽  
Tirumala Bharani Kumar Settypalli ◽  
Giovanni Cattoli ◽  
Charles Euloge Lamien ◽  
...  
Keyword(s):  
Middle East ◽  
Molecular Characterization ◽  
Disease Virus ◽  
Skin Disease ◽  
Clinical Signs ◽  
Sequence Alignments ◽  
Multiple Sequence ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

Abstract Background Lumpy skin disease (LSD) is a contagious viral disease of cattle caused by lumpy skin disease virus (LSDV). LSD has recently spread in Asia following outbreaks in the Middle East and Europe. The disease emerged in Bangladesh in July 2019 in the Chattogram district, then rapidly spread throughout the entire country. We investigated six LSD outbreaks in Bangladesh to record the clinical signs and collect samples for diagnostic confirmation. Furthermore, we performed the molecular characterization of Bangladesh isolates, analyzing the full RPO30 and GPCR genes and the partial EEV glycoprotein gene. Results Clinical observations revealed common LSD clinical signs in the affected cattle. PCR and real-time PCR, showed the presence of the LSDV genome in samples from all six districts. Phylogenetic analysis and detailed inspection of multiple sequence alignments revealed that Bangladesh isolates differ from common LSDV field isolates encountered in Africa, the Middle East, and Europe, as well as newly emerged LSDV variants in Russia and China. Instead, they were closely related to LSDV KSGP-0240, LSDV NI2490, and LSDV Kenya. Conclusions These results show the importance of continuous monitoring and characterization of circulating strains and the need to continually refine the strategies for differentiating vaccine strains from field viruses.

Molecular characterization of lumpy skin disease virus in Iran (2014–2018)

2021 ◽  
Author(s):  
Zeinab Hedayati ◽  
Hamid Reza Varshovi ◽  
Ali Mohammadi ◽  
Mohammad Tabatabaei
Keyword(s):  
Molecular Characterization ◽  
Disease Virus ◽  
Skin Disease ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

scholarly journals Molecular characterization of lumpy skin disease virus in Namibia, 2017

2018 ◽  
Vol 163 (9) ◽  
pp. 2525-2529
Author(s):  
Umberto Molini ◽  
Gottlieb Aikukutu ◽  
Siegfried Khaiseb ◽  
Naindji N. Haindongo ◽  
Angela C. Lilungwe ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Disease Virus ◽  
Skin Disease ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

Genomic characterization of lumpy skin disease virus in southern China

2021 ◽  
Author(s):  
Jun Ma ◽  
Yaoxian Yuan ◽  
Jianwei Shao ◽  
Minghui Sun ◽  
Wei He ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Southern China ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Genomic Characterization

scholarly journals Characterization of sheep pox virus vaccine for cattle against lumpy skin disease virus

2014 ◽  
Vol 109 ◽  
pp. 1-6 ◽  
Author(s):  
Eeva S.M. Tuppurainen ◽  
Caroline R. Pearson ◽  
Katarzyna Bachanek-Bankowska ◽  
Nick J. Knowles ◽  
Shadi Amareen ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Vaccine ◽  
Skin Disease ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

Full-length genome characterization of a novel recombinant vaccine-like lumpy skin disease virus strain detected during the climatic winter in Russia, 2019

2020 ◽  
Vol 165 (11) ◽  
pp. 2675-2677
Author(s):  
Alexander Sprygin ◽  
Antoinette Van Schalkwyk ◽  
Irina Shumilova ◽  
Alexander Nesterov ◽  
Svetlana Kononova ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Strain ◽  
Skin Disease ◽  
Recombinant Vaccine ◽  
Full Length ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Genome Characterization ◽  
Full Length Genome

scholarly journals Mechanical Transmission of Lumpy Skin Disease Virus by Stomoxys spp. (Stomoxys calsitrans, Stomoxys sitiens, Stomoxys indica), Diptera: Muscidae

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 477
Author(s):  
Arman Issimov ◽  
Lespek Kutumbetov ◽  
Mukhit B. Orynbayev ◽  
Berik Khairullin ◽  
Balzhan Myrzakhmetova ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Clinical Signs ◽  
Severe Case ◽  
Mechanical Transmission ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
First Time

Samples collected for PCR from recipient animals tested positive in 5 out of 6 cases, while the virus was isolated from 4 of 6 animals. The clinical signs exhibited by recipient animals were mostly moderate in nature with only one severe case. To our knowledge, this is the first time that transmission of LSDV by three Stomoxys species has been demonstrated, and their role as mechanical vectors of LSDV is indicated.

scholarly journals First Molecular Characterization of Poxviruses in Cattle, Sheep, and Goats in Botswana

2021 ◽  
Author(s):  
Boitumelo Magret Modise ◽  
Tirumala Bharani Kumar Settypalli ◽  
Tebogo Kgotlele ◽  
Dingrong Xue ◽  
Kebonyemodisa Ntesang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Clinical Symptoms ◽  
Phylogenetic Analyses ◽  
Small Ruminants ◽  
Virus Diseases ◽  
Sequence Alignments ◽  
Multiple Sequence ◽  
Lumpy Skin Disease ◽  
Multiple Sequence Alignments

Abstract BackgroundPoxviruses within the Capripoxvirus, Orthopoxvirus, and Parapoxvirus genera can infect livestock, with the two former presenting also zoonotic importance. In addition, they induce similar clinical symptoms in common host species, creating a challenge for diagnosis. Although endemic in the country, poxvirus infections of small ruminants and cattle have received little attention in Botswana, and there was no prior attempt of using molecular tools to diagnose the diseases and characterize the pathogens.MethodsA high-resolution melting (HRM) assay was used to detect and differentiate poxviruses in samples from four cattle (from Mahalapye, Kasane, and Molepolole), one sheep (from Jwaneng), and one goat (from Kasane). Molecular characterization of capripoxviruses and parapoxviruses was undertaken by sequence analysis of RPO30 and GPCR genes.ResultsThe HRM assay revealed lumpy skin disease virus (LSDV) in three cattle samples, pseudocowpox virus (PCPV) in one cattle sample, and orf virus (ORFV) in one goat and one sheep sample. The phylogenetic analyses, based on the RPO30 and GPCR, and the inspection of the multiple sequence alignments showed that the LSDV sequences of Botswana were more like those of common LSDV field isolates encountered in Africa, Asia, and Europe. The Botswana PCPV presented unique features and clustered between camel isolates and cattle isolates of the PCPV group. The Botswana ORFV from goat differed from the ORFV collected in sheep.ConclusionsThis study is the first report on the genetic characterization of pox virus diseases circulating in cattle, goats, and sheep in Botswana. It shows the importance of molecular methods to differentially diagnose pox virus diseases of ruminants.

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