scholarly journals Zonal characterization and differential trilineage potentials of equine intrasynovial deep digital flexor tendon-derived cells

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Vivian G. Quam ◽  
Nadine N. Altmann ◽  
Matthew T. Brokken ◽  
Sushmitha S. Durgam
Keyword(s):  
Chondrogenic Differentiation ◽  
Flexor Tendon ◽  
Adipogenic Differentiation ◽  
Tendon Healing ◽  
Surface Markers ◽  
In Vitro Proliferation ◽  
Tendon Regeneration ◽  
Chondrogenic Marker ◽  
Poor Outcomes

Abstract Background Intrasynovial deep digital flexor tendon (DDFT) injuries occur frequently and are often implicated in cases of navicular disease with poor outcomes and reinjuries. Cell-based approaches to tendon healing are gaining traction in veterinary medicine and ultimately may contribute to improved DDFT healing in horses. However, a better understanding of the innate cellular characteristics of equine DDFT is necessary for developing improved therapeutic strategies. Additionally, fibrocartilaginous, intrasynovial tendons like the DDFT are common sites of injury and share a poor prognosis across species, offering translational applications of this research. The objective of this study is to isolate and characterize tendon-derived cells (TDC) from intrasynovial DDFT harvested from within the equine forelimb podotrochlear bursa. TDC from the fibrocartilaginous and tendinous zones are separately isolated and assessed. Flow cytometry is performed for mesenchymal stem cell (MSC) surface markers (CD 29, CD 44, CD 90). Basal tenogenic, osteogenic and chondrogenic markers are assessed via quantitative real time-PCR, and standard trilineage differentiation is performed with third passage TDC from the fibrocartilaginous (fTDC) and tendinous (tTDC) zones of DDFT. Results Low-density plating isolated homogenous TDC populations from both zones. During monolayer passage, both TDC subpopulations exhibited clonogenicity, high in vitro proliferation rate, and fibroblast-like morphology. fTDC and tTDC were positive for MSC surface markers CD90 and CD29 and negative for CD44. There were no significant differences in basal tenogenic, osteogenic or chondrogenic marker expression between zones. While fTDC were largely restricted to chondrogenic differentiation, tTDC underwent osteogenic and chondrogenic differentiation. Both TDC subpopulations displayed weak adipogenic differentiation potentials. Conclusions TDC at the level of the podotrochlear bursa, that potentially could be targeted for enhancing DDFT injury healing in horses were identified and characterized. Pending further investigation, promoting chondrogenic properties in cells administered exogenously into the intrasynovial space may be beneficial for intrasynovial tendon regeneration.

scholarly journals Zonal Characterization and Differential Trilineage Potentials of Equine Intrasynovial Deep Digital Flexor Tendon-Derived Cells

2020 ◽  
Author(s):  
Vivian G Quam ◽  
Nadine N Altmann ◽  
Matthew T Brokken ◽  
Sushmitha Durgam
Keyword(s):  
Chondrogenic Differentiation ◽  
Flexor Tendon ◽  
Adipogenic Differentiation ◽  
Tendon Repair ◽  
Cell Surface Antigens ◽  
In Vitro Proliferation ◽  
Repair Capacity ◽  
Tendon Regeneration ◽  
Poor Outcomes

Abstract Background: Intrasynovial deep digital flexor tendon (DDFT) injury is a common cause for forelimb lameness in horses and are typically associated with poor outcomes. Intrasynovial tendon contains a superficial fibrocartilage in response to compressive forces from an opposing boney prominence, and is critical for tendon gliding function. Characterization of tendon-derived cells (TDC) from intrasynovial tendon fibrocartilage has not been conducted as for prototypical extrasynovial tendon and is necessary for developing improved therapeutic strategies. Results: In this study, we successfully isolated homogenous TDC from the fibrocartilaginous (fTDC) and tendinous (tTDC) zones of equine forelimb intrasynovial DDFT via low-density plating method. During monolayer passage, both TDC subpopulations exhibited clonogenicity, high in vitro proliferation rate, and fibroblast-like morphology. Third passage fTDC and tTDC were positive for cell surface antigens CD90 and CD29 and negative for CD44 and CD45. There were no significant differences in the basal tenogenic, osteogenic and chondrogenic marker expressions of fTDC and tTDC. Trilineage differentiation demonstrated that fTDC were largely restricted to chondrogenic differentiation; whereas, those from the tendinous zone underwent osteogenic and chondrogenic differentiation. Both TDC subpopulations displayed weak adipogenic differentiation potentials. Conclusions: These results provide a foundation for studies exploring cell-based therapies for intrasynovial tendon repair as these TDC are potential targets to enhance intrinsic repair capacity. Pending further investigation, promoting chondrogenic properties in cells administered exogenously into the intrasynovial space may be beneficial for intrasynovial tendon regeneration.

An in vitro model of fibroblast activity and adhesion formation during flexor tendon healing

1994 ◽  
Vol 19 (5) ◽  
pp. 769-776 ◽  
Author(s):  
David L. Packer ◽  
George W. Dombi ◽  
Ping Yang Yu ◽  
Paul Zidel ◽  
Walter G. Sullivan
Keyword(s):  
In Vitro Model ◽  
Flexor Tendon ◽  
Adhesion Formation ◽  
Tendon Healing ◽  
Vitro Model

Flexor tendon healing in vitro: Effects of TGF-β on tendon cell collagen production

2002 ◽  
Vol 27 (4) ◽  
pp. 615-620 ◽  
Author(s):  
Matthew B. Klein ◽  
Naveen Yalamanchi ◽  
Hung Pham ◽  
Michael T. Longaker ◽  
James Chan
Keyword(s):  
Flexor Tendon ◽  
Tendon Healing ◽  
Collagen Production ◽  
Tendon Cell ◽  
In Vitro Effects

Effect of Hyaluronic Acid on Rabbit Profundus Flexor Tendon Healing in Vitro

1993 ◽  
Vol 55 (4) ◽  
pp. 411-415 ◽  
Author(s):  
Nader I. Salti ◽  
Robert J. Tuel ◽  
Daniel P. Mass
Keyword(s):  
Hyaluronic Acid ◽  
Flexor Tendon ◽  
Tendon Healing

scholarly journals Effects of antithrombotic drugs fondaparinux and tinzaparin on in vitro proliferation and osteogenic and chondrogenic differentiation of bone‐derived mesenchymal stem cells

2011 ◽  
Vol 29 (9) ◽  
pp. 1327-1335 ◽  
Author(s):  
Argiris Papathanasopoulos ◽  
Dimitrios Kouroupis ◽  
Karen Henshaw ◽  
Dennis McGonagle ◽  
Elena A. Jones ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Antithrombotic Drugs ◽  
In Vitro Proliferation

scholarly journals In vitro Comparison of Adipogenic Differentiation in Human Adipose-Derived Stem Cells Cultured with Collagen Gel and Platelet-Rich Fibrin

2021 ◽  
Vol 54 (03) ◽  
pp. 278-283
Author(s):  
Pallavi Priyadarshini ◽  
Soumi Samuel ◽  
Basan Gowda Kurkalli ◽  
Chethan Kumar ◽  
Basavarajappa Mohana Kumar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Soft Tissue ◽  
Cell Surface ◽  
Adipogenic Differentiation ◽  
Collagen Gel ◽  
Surface Markers ◽  
Adipose Derived Stem Cells ◽  
Cell Surface Markers ◽  
Platelet Rich Fibrin

Abstract Background: Adipose-derived stem cells (ADSCs) are the most preferred cell type, based on their phenotypic characteristics, plasticity, and favorable immunological properties for applications in soft-tissue augmentation. Hence, the present in vitro study was aimed to evaluate the adipogenic differentiation potential of human ADSCs upon culturing individually with collagen gel and platelet-rich fibrin (PRF). Materials and methods: The collected lipoaspirate was used for establishing ADSCs using enzymatic digestion method. Then, the cells were analyzed for their morphology, viability, proliferation rate, population doubling time (PDT), colony-forming ability, cell surface markers expression, and osteogenic differentiation as biological properties. Further, ADSCs were evaluated for their adipogenicity using induction media alone, and by culturing with collagen gel and PRF individually for prospective tissue augmentation. Results: ADSCs were successfully established in vitro and exhibited a fibroblast-like morphology throughout the culture period. Cells had higher viability, proliferation potential and showed their ability to form colonies. The positive expression of cell surface markers and osteogenic ability confirmed the potency of ADSCs. The ADSCs cultured on collagen gel and PRF, individually, showed higher number of differentiated adipocytes than ADSCs grown with adipogenic induction medium alone. Conclusion: The extent of lipid accumulation by ADSCs was slightly higher when cultured on collagen gel than on PRF. Additional experiments are required to confirm better suitability of scaffold materials for soft-tissue regeneration.

scholarly journals Exosomes derived from M0, M1 and M2 macrophages exert distinct influences on the proliferation and differentiation of mesenchymal stem cells

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8970 ◽  
Author(s):  
Yu Xia ◽  
Xiao-Tao He ◽  
Xin-Yue Xu ◽  
Bei-Min Tian ◽  
Ying An ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Adipogenic Differentiation ◽  
Underlying Mechanism ◽  
Suppressive Effect ◽  
Complete Medium ◽  
Marrow Mesenchymal Stem Cells

Background Different phenotypes of macrophages (M0, M1 and M2 Mφs) have been demonstrated to play distinct roles in regulating mesenchymal stem cells in various in vitro and in vivo systems. Our previous study also found that cell-conditioned medium (CM) derived from M1 Mφs supported the proliferation and adipogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs), whereas CM derived from either M0 or M2 Mφs showed an enhanced effect on cell osteogenic differentiation. However, the underlying mechanism remains incompletely elucidated. Exosomes, as key components of Mφ-derived CM, have received increasing attention. Therefore, it is possible that exosomes may modulate the effect of Mφ-derived CM on the property of BMMSCs. This hypothesis was tested in the present study. Methods In this study, RAW264.7 cells were induced toward M1 or M2 polarization with different cytokines, and exosomes were isolated from the unpolarized (M0) and polarized (M1 and M2) Mφs. Mouse BMMSCs were then cultured with normal complete medium or inductive medium supplemented with M0-Exos, M1-Exos or M2-Exos. Finally, the proliferation ability and the osteogenic, adipogenic and chondrogenic differentiation capacity of the BMMSCs were measured and analyzed. Results We found that only the medium containing M1-Exos, rather than M0-Exos or M2-Exos, supported cell proliferation and osteogenic and adipogenic differentiation. This was inconsistent with CM-based incubation. In addition, all three types of exosomes had a suppressive effect on chondrogenic differentiation. Conclusion Although our data demonstrated that exosomes and CM derived from the same phenotype of Mφs didn’t exert exactly the same cellular influences on the cocultured stem cells, it still confirmed the hypothesis that exosomes are key regulators during the modulation effect of Mφ-derived CM on BMMSC property.

Sign in / Sign up

Export Citation Format

Share Document