scholarly journals Down-regulation of microRNA-144-3p and its clinical value in non-small cell lung cancer: a comprehensive analysis based on microarray, miRNA-sequencing, and quantitative real-time PCR data

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Yu-Ji Chen ◽  
Yi-Nan Guo ◽  
Ke Shi ◽  
Hui-Mei Huang ◽  
Shu-Ping Huang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Real Time ◽  
Real Time Pcr ◽  
Comprehensive Analysis ◽  
Small Cell ◽  
Quantitative Real Time Pcr ◽  
Small Cell Lung ◽  
Clinical Value ◽  
Mirna Sequencing

scholarly journals Determination of EGFR gene somatic mutations in tissues and plasma of patients with advanced non-small cell lung cancer

2016 ◽  
Vol 62 (6) ◽  
pp. 638-644
Author(s):  
O.I. Brovkina ◽  
M.G. Gordiev ◽  
A.N. Toropovskiy ◽  
D.S. Khodyrev ◽  
R.F. Enikeev ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Real Time ◽  
Cell Lung Cancer ◽  
Real Time Pcr ◽  
Test System ◽  
Small Cell ◽  
Egfr Mutations ◽  
Small Cell Lung ◽  
Egfr Gene

The presence of activating mutations in the EGFR gene influences cell proliferation, angiogenesis, and increases metastatic ability; it has a significant impact on the choice of medical therapy of non-small cell lung cancer (NSCLC). The use of targeted therapy with tyrosine kinase inhibitors requires performance of appropriate genetic tests. The aim of this study was to design a real-time PCR-based diagnostic kit for fast and cheap of EGFR mutations testing in paraffin blocks and plasma, and kit validation using samples from patients with NSCLC, and also comparative estimation of diagnostic features of real-time PCR with wild type blocking and digital PCR for mutation testing in blood plasma. The study included 156 patients with various types of adenocarcinoma differentiation. It was designed a simple and efficient real-time PCR-based method of detecting L858R activating mutation and del19 deletion in the EGFR gene for DNA isolated from paraffin blocks. Kit for EGFR mutations was validated using 411 samples of paraffin blocks. The proposed system showed high efficiency for DNA testing from paraffin blocks: a concordance with results of testing with therascreen® EGFR RGQ PCR Kit (`Qiagen`, Germany) was 100%. It has been shown the possibility of using this test system for the detection of mutations in plasma

Comparative Quantification of Plasma hnRNP B1 mRNA in Non-small Cell Lung Cancer Patients by Real-time PCR

2009 ◽  
Vol 29 (3) ◽  
pp. 249-255 ◽  
Author(s):  
Jeong-Man Kim ◽  
Sang Hyun Hwang ◽  
Eun Ju Song ◽  
Lee Sang-Yull ◽  
Yeong-Dae Kim ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Real Time ◽  
Cancer Patients ◽  
Cell Lung Cancer ◽  
Real Time Pcr ◽  
Small Cell ◽  
Small Cell Lung ◽  
Lung Cancer Patients

scholarly journals Real-time PCR quantification of plasma DNA in non-small cell lung cancer patients and healthy controls

2009 ◽  
Vol 14 (Suppl 4) ◽  
pp. 237 ◽  
Author(s):  
A Szpechcinski ◽  
M Dancewicz ◽  
P Kopinski ◽  
J Kowalewski ◽  
J Chorostowska-Wynimko
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Real Time ◽  
Cancer Patients ◽  
Real Time Pcr ◽  
Small Cell ◽  
Healthy Controls ◽  
Plasma Dna ◽  
Small Cell Lung ◽  
Lung Cancer Patients
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